FastQCFastQC Report
Fri 27 May 2016
SRR2140288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6295615
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75600.025.0601921
GTACATG224600.024.798421
TACATGG224800.024.4019282
GTATCAA110500.024.4004821
ACATGGG234050.023.202233
CAACGCA110450.022.3851835
TCAACGC113000.022.2166024
AACGCAG115050.021.709346
ATCAACG115300.021.628083
GTACTAG15550.021.436631
CATGGGG185750.021.1929764
TATCAAC126000.020.7968772
ATGGGGG116800.017.8809725
ACGCAGA138050.017.8482177
GAGTACA163250.017.73091
CGCAGAG142150.017.2736538
AGTACAT164150.016.9660262
AGAGTAC128100.015.06744810-11
CGTCGAA5104.0617124E-715.04667894
GTACTGG43450.014.275371
Produced by FastQC (version 0.11.5)