FastQCFastQC Report
Fri 27 May 2016
SRR2140287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14125285
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147250.042.7650221
GTATCAA257800.032.327731
TCAACGC274950.029.1251184
ATCAACG275350.029.0676363
CAACGCA279250.028.6463135
AACGCAG293250.027.423126
TATCAAC306500.026.4174212
ACGCAGA346950.023.0565877
CGCAGAG352800.022.6502658
GTACATG352550.021.4532831
TACATGG355900.020.7515952
ACATGGG362900.019.9195543
GCAGAGT430300.018.3149179
GTGGTAT85550.018.0183241
CATGGGG282400.018.0097734
GAGTACT239450.017.6608512-13
AGAGTAC378150.016.77013410-11
CAGAGTA422250.016.59824210-11
GAGTACA272700.015.6999781
GTACTTT276900.015.34169214-15
Produced by FastQC (version 0.11.5)