##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14125285
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960206820605745	31.0	31.0	34.0	30.0	34.0
2	32.104488298820165	33.0	31.0	34.0	30.0	34.0
3	32.00542105876094	33.0	31.0	34.0	30.0	34.0
4	35.914191182691184	37.0	35.0	37.0	35.0	37.0
5	35.77234271733278	37.0	35.0	37.0	35.0	37.0
6	35.830216098294656	37.0	35.0	37.0	35.0	37.0
7	35.78008953447665	37.0	35.0	37.0	33.0	37.0
8	35.77140864768392	37.0	35.0	37.0	33.0	37.0
9	37.515409635982564	39.0	37.0	39.0	35.0	39.0
10-11	37.387579294860245	39.0	37.0	39.0	34.0	39.0
12-13	37.361476423307565	39.0	37.0	39.0	34.0	39.0
14-15	38.62866575789444	40.0	38.0	41.0	34.0	41.0
16-17	38.47174722492325	40.0	38.0	41.0	34.0	41.0
18-19	38.530752512250196	40.0	38.0	41.0	34.0	41.0
20-21	38.556220927260576	40.0	38.0	41.0	34.0	41.0
22-23	38.47948198913496	40.0	38.0	41.0	34.0	41.0
24-25	38.419749638403204	40.0	38.0	41.0	34.0	41.0
26-27	38.20294870929747	40.0	38.0	41.0	33.5	41.0
28-29	38.08426228642264	40.0	37.5	41.0	33.0	41.0
30-31	37.960094551762964	40.0	37.0	41.0	33.0	41.0
32-33	37.775027053094405	40.0	37.0	41.0	33.0	41.0
34-35	37.87301185005032	40.0	37.0	41.0	33.0	41.0
36-37	37.84846805176578	40.0	37.0	41.0	33.0	41.0
38-39	37.80342158380184	40.0	37.0	41.0	33.0	41.0
40-41	37.729071081073705	40.0	37.0	41.0	33.0	41.0
42-43	37.79358610197549	40.0	37.0	41.0	33.0	41.0
44-45	37.799208745720534	40.0	37.0	41.0	33.0	41.0
46-47	37.73275412646419	40.0	37.0	41.0	32.5	41.0
48-49	37.63893811975075	40.0	37.0	41.0	32.0	41.0
50-51	37.51869823170499	40.0	36.0	41.0	32.0	41.0
52-53	37.33684719489807	39.0	36.0	41.0	31.0	41.0
54-55	37.1854476261544	39.0	36.0	41.0	31.0	41.0
56-57	37.04776473003922	39.0	35.0	41.0	31.0	41.0
58-59	36.875650523152885	39.0	35.0	40.0	31.0	41.0
60-61	36.6855691384776	38.5	35.0	40.0	31.0	41.0
62-63	36.46951521596	38.0	35.0	40.0	30.5	41.0
64-65	36.16107538321121	38.0	34.5	40.0	30.0	41.0
66-67	35.863407992326636	37.0	34.0	40.0	29.5	41.0
68-69	35.54702508568694	37.0	34.0	39.0	29.0	41.0
70-71	35.234242835040746	36.0	34.0	39.0	29.0	40.5
72-73	34.95003838421816	36.0	34.0	39.0	29.0	40.0
74-75	34.61948602263922	35.0	33.5	38.0	29.0	40.0
76-77	33.36189943190733	34.5	31.5	36.5	27.0	39.0
78-79	34.08865590509626	35.0	33.0	37.0	29.0	39.0
80-81	34.01111500506862	35.0	33.0	36.5	29.0	39.0
82-83	33.787954388601015	35.0	33.0	36.0	29.0	37.5
84-85	33.55214539181566	35.0	33.0	36.0	29.0	37.0
86-87	33.355894867729035	35.0	33.0	35.0	29.0	37.0
88-89	33.190497743431436	35.0	33.0	35.0	29.0	36.0
90-91	33.061763224965276	35.0	33.0	35.0	29.0	36.0
92-93	33.014942025245354	35.0	33.0	35.0	29.0	36.0
94-95	32.94858447128317	34.0	33.0	35.0	29.0	35.5
96-97	32.997761540206014	34.0	32.5	35.0	29.0	35.0
98-99	33.1268856275499	34.0	33.0	35.0	29.0	35.0
100	31.846582303574028	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	10.0
20	95.0
21	571.0
22	2712.0
23	9187.0
24	24491.0
25	51020.0
26	91312.0
27	142365.0
28	202508.0
29	267377.0
30	336163.0
31	414662.0
32	508544.0
33	639995.0
34	843797.0
35	1151554.0
36	1750917.0
37	2857713.0
38	3295867.0
39	1483583.0
40	50838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61146483062112	20.403821940583853	13.639738950399938	24.34497427839509
2	15.196493380487544	21.450880460111073	40.0615492006002	23.291076958801185
3	18.48282223877258	25.68659364371862	30.45636746703065	25.37421665047815
4	12.879088811305401	17.616515348185896	38.34260335278191	31.161792487726796
5	13.220887224576353	37.84384527462632	35.76974907054973	13.165518430247602
6	30.85837206116549	37.61289772206366	18.293216738635714	13.23551347813513
7	27.064827364545213	32.3346042221449	22.97974164769065	17.620826765619242
8	24.896495893711172	36.60534990975403	20.984220849349235	17.513933347185564
9	25.58094934013721	16.453480407651952	21.15085111557041	36.81471913664043
10-11	24.577383748363307	26.599470382367507	28.779387460146822	20.043758409122365
12-13	25.019335893045696	24.04684932020841	29.02532586068175	21.908488926064145
14-15	22.031548389997084	25.600318860822984	26.418001477492314	25.950131271687617
16-17	21.965729434373934	28.7245197016797	27.143690863253273	22.16606000069309
18-19	21.93449544301427	28.08700406396789	28.69107525323028	21.28742523978756
20-21	22.565717088074777	27.298911342418563	28.54062212584975	21.5947494436569
22-23	22.392022915517494	27.229697645152385	28.361266555954074	22.01701288337605
24-25	22.212624102857152	27.30139984134834	28.43558358025425	22.05039247554026
26-27	22.18924696484911	27.44670782350086	28.338410887602826	22.0256343240472
28-29	22.109391463614134	27.627913835386593	28.29892787837035	21.963766822628923
30-31	22.327321451312265	27.44799097963993	28.25414822627132	21.970539342776483
32-33	22.123087960542552	27.545792308995836	28.22532956004994	22.10579017041167
34-35	22.264762973870177	27.53038625683305	28.27955427551242	21.92529649378435
36-37	22.27340388265082	27.527079884506257	28.18868978500771	22.010826447835214
38-39	22.178574062696928	27.660893630142574	28.19338473035246	21.96714757680803
40-41	22.339611904786764	27.51442547908428	28.073916629153516	22.072045986975443
42-43	22.242062740500295	27.643101288089532	27.888466389775896	22.22636958163427
44-45	22.331962360632005	27.687989522725836	27.80295586630221	22.177092250339957
46-47	22.299391653535228	27.697533577912164	27.64863626816854	22.35443850038407
48-49	22.281326788823765	27.848311388229558	27.581239403337676	22.289122419609
50-51	22.299681494417197	27.804756126500134	27.59166894922562	22.30389342985704
52-53	22.413361360895358	27.790844790594683	27.44437220599344	22.351421642516524
54-55	22.390309759742273	27.71814916060903	27.496062440670173	22.395478638978524
56-57	22.42335062654494	27.76477946164706	27.46830575970513	22.34356415210287
58-59	22.34273691079745	27.899511699186746	27.477138545149582	22.280612844866226
60-61	22.44619742947183	27.79084955261853	27.519307729783716	22.243645288125926
62-63	22.45099952085738	27.875236405560894	27.382689152259974	22.29107492132175
64-65	22.35228168217875	27.87337000301809	27.41227447524649	22.362073839556675
66-67	22.440971197320557	27.824069630737615	27.308704304979347	22.426254866962484
68-69	22.304016214731924	27.857340564744863	27.43323203872644	22.40541118179677
70-71	22.294366141268252	27.722054659070412	27.44473381456858	22.53884538509276
72-73	22.38593082335297	27.76439657443413	27.393264760109375	22.45640784210352
74-75	22.301736392033288	27.8420417612869	27.4224579047519	22.433763941927907
76-77	22.379937981004314	27.763886739442945	27.348571917575658	22.50760336197708
78-79	22.263762308156092	27.7861822082343	27.336748374685644	22.613307108923962
80-81	22.323666120157732	27.825007870873208	27.38221637411598	22.46910963485308
82-83	22.284200248540134	27.842922528638503	27.48698495408156	22.385892268739802
84-85	22.43422235256962	27.797634500123646	27.365148486236084	22.40299466107065
86-87	22.286536542682985	27.832649120606156	27.446359160622702	22.434455176088154
88-89	22.321687798844856	27.76558160069658	27.414170583746838	22.498560016711725
90-91	22.34833476758564	27.822442234086747	27.402399311106617	22.426823687220995
92-93	22.371996470656715	27.760766927141116	27.499658534308224	22.367578067893945
94-95	22.169738584174404	27.897029704915198	27.535024377595395	22.398207333315
96-97	22.179060828948945	27.998256932045418	27.63057473684946	22.19210750215617
98-99	23.29016795449455	29.99568478175473	27.781139758482716	18.93300750526801
100	22.316390358087173	30.97408163090676	21.44340847874514	25.266119532260934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2094.0
1	1930.5
2	2868.5
3	4220.0
4	5532.5
5	8896.5
6	12009.5
7	14159.5
8	15468.5
9	15091.0
10	13751.5
11	12191.5
12	12495.5
13	16109.0
14	25287.0
15	38322.5
16	49759.5
17	57085.5
18	60139.5
19	59679.0
20	56928.0
21	53890.5
22	53517.0
23	57642.5
24	66916.5
25	82976.5
26	105083.0
27	129947.5
28	157336.5
29	189221.0
30	223453.0
31	258156.5
32	293904.5
33	329912.0
34	363235.5
35	393238.5
36	427058.5
37	461480.0
38	487817.0
39	506141.5
40	523265.5
41	541094.5
42	554448.0
43	567221.0
44	581009.0
45	590232.5
46	595694.5
47	599164.0
48	597761.0
49	592012.0
50	581625.5
51	565792.0
52	545412.0
53	518407.5
54	488486.0
55	454771.5
56	418788.5
57	380083.0
58	336797.5
59	290939.5
60	241968.0
61	197656.0
62	158541.0
63	123275.5
64	94230.0
65	71537.5
66	54164.0
67	41927.0
68	32560.5
69	24260.0
70	17778.5
71	13004.5
72	9422.0
73	6516.0
74	4367.5
75	2881.0
76	1847.0
77	1146.5
78	719.0
79	470.0
80	312.0
81	218.0
82	163.5
83	111.5
84	75.5
85	55.5
86	46.5
87	42.0
88	31.0
89	19.5
90	12.5
91	9.5
92	6.0
93	2.0
94	0.5
95	0.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.1857764285817946E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.309546674633468E-4
18-19	0.0038406304722347195
20-21	0.0
22-23	0.001246147254318086
24-25	5.665592777162392E-4
26-27	0.013610095559378647
28-29	0.007500088345794906
30-31	5.925634914039617E-4
32-33	0.006810585427046279
34-35	0.004614185173913984
36-37	0.0019592278954581738
38-39	0.0014477857679425744
40-41	0.003832751304219162
42-43	0.00865466236039359
44-45	0.00947437049159719
46-47	0.008337722725432147
48-49	0.007713172383151857
50-51	0.03312191771479314
52-53	0.01199213406654437
54-55	6.089986554832183E-5
56-57	0.00733164902451834
58-59	0.006417667618188204
60-61	7.817521536099204E-5
62-63	0.005080720739683514
64-65	0.0021105058472360915
66-67	0.021882582864139216
68-69	0.029460590938047503
70-71	0.014248665287951991
72-73	0.010591232905205137
74-75	0.01557527610458558
76-77	0.0021457337215124578
78-79	0.0026681768231384983
80-81	0.001301784200087901
82-83	0.0215368640651865
84-85	7.274732845540043E-4
86-87	0.008074016679770236
88-89	8.565055112194351E-4
90-91	0.003128152001805283
92-93	0.0010565634103254292
94-95	7.710018068451897E-4
96-97	0.0025019003553863354
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	260.0
20-21	1035.0
22-23	2324.0
24-25	5530.0
26-27	7053.0
28-29	12450.0
30-31	23390.0
32-33	30888.0
34-35	42744.0
36-37	62822.0
38-39	75959.0
40-41	83172.0
42-43	92852.0
44-45	109714.0
46-47	110768.0
48-49	104025.0
50-51	98239.0
52-53	98984.0
54-55	108704.0
56-57	115451.0
58-59	117595.0
60-61	117039.0
62-63	117449.0
64-65	125871.0
66-67	134316.0
68-69	136614.0
70-71	137451.0
72-73	134194.0
74-75	139767.0
76-77	141309.0
78-79	152032.0
80-81	156762.0
82-83	154932.0
84-85	158522.0
86-87	170115.0
88-89	182991.0
90-91	197734.0
92-93	220273.0
94-95	271065.0
96-97	580951.0
98-99	977675.0
100-101	8414264.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.97569537828663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9500592414472	72.0976726487914
2	7.917463752627155	12.980791935079793
3	2.204899561307203	5.422445243323113
4	0.8804959448991224	2.887170694434684
5	0.398350013707636	1.6327509688816737
6	0.2179282378566014	1.071889130451573
7	0.12880524006588215	0.7391229385937486
8	0.08063254560361405	0.5287927196782136
9	0.05370778574696632	0.39624597754520363
>10	0.1646632850819466	2.0102205807741598
>50	0.0023060081607304663	0.12693192336440726
>100	6.572292039321772E-4	0.08388694240150689
>500	3.11542920699314E-5	0.02207829668052802
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.079503174626211E-6	0.0	7.079503174626211E-6	0.0
3	0.0	7.079503174626211E-6	0.0	7.079503174626211E-6	0.0
4	0.0	2.1238509523878634E-5	0.0	1.4159006349252422E-5	0.0
5	0.0	2.1238509523878634E-5	0.0	2.8318012698504844E-5	0.0
6	0.0	2.1238509523878634E-5	0.0	4.247701904775727E-5	7.079503174626211E-6
7	0.0	2.1238509523878634E-5	0.0	4.247701904775727E-5	7.079503174626211E-6
8	0.0	2.8318012698504844E-5	0.0	5.663602539700969E-5	1.4159006349252422E-5
9	0.0	1.2035155396864559E-4	0.0	7.787453492088833E-5	4.247701904775727E-5
10-11	0.0	1.2035155396864559E-4	0.0	9.557329285745385E-5	4.247701904775727E-5
12-13	0.0	1.238913055559587E-4	0.0	1.0973229920670627E-4	4.247701904775727E-5
14-15	0.0	1.7344782777834218E-4	0.0	1.238913055559587E-4	9.557329285745385E-5
16-17	0.0	1.840670825402815E-4	0.0	1.274310571432718E-4	1.3451056031789802E-4
18-19	0.0	1.982260888895339E-4	0.0	1.4512981507983734E-4	1.6636832460371597E-4
20-21	7.079503174626211E-6	2.2654410158803875E-4	0.0	1.6636832460371597E-4	1.7344782777834218E-4
22-23	7.079503174626211E-6	2.548621142865436E-4	0.0	1.840670825402815E-4	2.0530559206416012E-4
24-25	7.079503174626211E-6	2.7964037539773536E-4	0.0	2.2300435000072565E-4	2.584018658738567E-4
26-27	7.079503174626211E-6	2.8318012698504846E-4	0.0	2.7964037539773536E-4	2.7256087222310915E-4
28-29	7.079503174626211E-6	2.8318012698504846E-4	0.0	5.451217444462183E-4	2.7610062381042225E-4
30-31	7.079503174626211E-6	2.973391333343009E-4	0.0	0.001472536660322252	2.8318012698504846E-4
32-33	7.079503174626211E-6	2.973391333343009E-4	0.0	0.003047726116676584	3.114981396835533E-4
34-35	7.079503174626211E-6	3.4689565555668435E-4	0.0	0.00512910005001669	3.185776428581795E-4
36-37	7.079503174626211E-6	4.0707143254100714E-4	0.0	0.008573278344472341	3.256571460328057E-4
38-39	7.079503174626211E-6	4.2477019047757267E-4	0.0	0.013815650445283052	3.5397515873131056E-4
40-41	7.079503174626211E-6	4.530882031760775E-4	0.0	0.019663320067524304	3.610546619059368E-4
42-43	7.079503174626211E-6	4.743267126999562E-4	0.0	0.025344621365161836	3.610546619059368E-4
44-45	7.079503174626211E-6	5.097242285730872E-4	0.0	0.03182944627311944	3.68134165080563E-4
46-47	7.079503174626211E-6	5.309627380969659E-4	0.0	0.03901160224377774	3.822931714298154E-4
48-49	7.079503174626211E-6	5.380422412715921E-4	0.0	0.0465654321311039	4.1061118412832025E-4
50-51	7.079503174626211E-6	5.840590119066625E-4	0.0	0.054108642763668134	4.1415093571563335E-4
52-53	7.079503174626211E-6	5.875987634939756E-4	0.0	0.06205892482877337	4.6724720952533E-4
54-55	7.079503174626211E-6	5.875987634939756E-4	0.0	0.06966231123832192	5.203434833350266E-4
56-57	7.079503174626211E-6	5.875987634939756E-4	0.0	0.07604802310183476	5.274229865096528E-4
58-59	7.079503174626211E-6	5.911385150812887E-4	0.0	0.0825434672645543	5.309627380969659E-4
60-61	7.079503174626211E-6	6.123770246051673E-4	0.0	0.08860352198203433	5.34502489684279E-4
62-63	7.079503174626211E-6	6.300757825417328E-4	0.0	0.09505648912570613	5.875987634939756E-4
64-65	7.079503174626211E-6	6.796323047641163E-4	0.0	0.10172892086779134	5.875987634939756E-4
66-67	7.079503174626211E-6	6.796323047641163E-4	0.0	0.10827746130432059	5.946782666686018E-4
68-69	7.079503174626211E-6	7.008708142879949E-4	0.0	0.11466317316783342	6.01757769843228E-4
70-71	7.079503174626211E-6	7.256490753991867E-4	0.0	0.12006483409007322	6.229962793671066E-4
72-73	7.079503174626211E-6	7.504273365103784E-4	0.0	0.1245143018353258	7.150298206372473E-4
74-75	7.079503174626211E-6	7.71665846034257E-4	0.0	0.1282947565305762	7.150298206372473E-4
76-77	7.079503174626211E-6	7.858248523835094E-4	0.0	0.1318026503536035	7.256490753991867E-4
78-79	7.079503174626211E-6	7.929043555581357E-4	0.0	0.1341070286369443	7.36268330161126E-4
80-81	7.079503174626211E-6	7.929043555581357E-4	0.0	0.13492471125361366	7.681260944469439E-4
82-83	7.079503174626211E-6	8.247621198439536E-4	0.0	0.1355335485266315	8.318416230185798E-4
84-85	7.079503174626211E-6	8.353813746058929E-4	0.0	0.13576363237980685	8.353813746058929E-4
86-87	7.079503174626211E-6	8.566198841297715E-4	0.0	0.13585212616948966	8.353813746058929E-4
88	7.079503174626211E-6	8.920174000029026E-4	0.0	0.13585566592107698	8.495403809551453E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14725	0.0	42.765022	1
GTATCAA	25780	0.0	32.32773	1
TCAACGC	27495	0.0	29.125118	4
ATCAACG	27535	0.0	29.067636	3
CAACGCA	27925	0.0	28.646313	5
AACGCAG	29325	0.0	27.42312	6
TATCAAC	30650	0.0	26.417421	2
ACGCAGA	34695	0.0	23.056587	7
CGCAGAG	35280	0.0	22.650265	8
GTACATG	35255	0.0	21.453283	1
TACATGG	35590	0.0	20.751595	2
ACATGGG	36290	0.0	19.919554	3
GCAGAGT	43030	0.0	18.314917	9
GTGGTAT	8555	0.0	18.018324	1
CATGGGG	28240	0.0	18.009773	4
GAGTACT	23945	0.0	17.66085	12-13
AGAGTAC	37815	0.0	16.770134	10-11
CAGAGTA	42225	0.0	16.598242	10-11
GAGTACA	27270	0.0	15.699978	1
GTACTTT	27690	0.0	15.341692	14-15
>>END_MODULE