FastQCFastQC Report
Fri 27 May 2016
SRR2140286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6394945
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72550.045.736391
GTATCAA132100.034.1651081
ATCAACG151650.028.812913
TCAACGC151850.028.747924
CAACGCA156300.027.9294385
TATCAAC159700.027.6176852
AACGCAG160050.027.3776826
ACGCAGA186150.023.539077
CGCAGAG190850.022.8948278
GTACATG183000.021.0045171
TACATGG181600.020.646332
ACATGGG183850.019.7235453
GCAGAGT228100.019.1379729
GAGTACT127000.018.72399112-13
GTGGTAT41700.018.3174421
CATGGGG136850.018.1851024
CAGAGTA219550.017.1059310-11
TGGTATC40450.017.0560592
AGAGTAC200300.016.90464810-11
GTACTTT146900.016.31377814-15
Produced by FastQC (version 0.11.5)