##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6394945
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05407239624422	33.0	31.0	34.0	30.0	34.0
2	32.19070687863617	33.0	31.0	34.0	30.0	34.0
3	32.09171650420762	33.0	31.0	34.0	30.0	34.0
4	35.9750346562793	37.0	35.0	37.0	35.0	37.0
5	35.8450571506088	37.0	35.0	37.0	35.0	37.0
6	35.90145998128209	37.0	35.0	37.0	35.0	37.0
7	35.85640752187861	37.0	35.0	37.0	35.0	37.0
8	35.84957587594577	37.0	35.0	37.0	35.0	37.0
9	37.60427884837164	39.0	37.0	39.0	35.0	39.0
10-11	37.48677721231379	39.0	37.0	39.0	34.5	39.0
12-13	37.46069215607015	39.0	37.0	39.0	34.0	39.0
14-15	38.754061684658744	40.0	38.0	41.0	34.5	41.0
16-17	38.60074754356762	40.0	38.0	41.0	34.0	41.0
18-19	38.675429890327436	40.0	38.0	41.0	34.0	41.0
20-21	38.70685615329934	40.0	38.0	41.0	34.0	41.0
22-23	38.633858554751605	40.0	38.0	41.0	34.0	41.0
24-25	38.57647254083656	40.0	38.0	41.0	34.0	41.0
26-27	38.36947994232408	40.0	38.0	41.0	34.0	41.0
28-29	38.25866689210311	40.0	38.0	41.0	34.0	41.0
30-31	38.133223895720974	40.0	38.0	41.0	33.0	41.0
32-33	37.950555435690674	40.0	37.0	41.0	33.0	41.0
34-35	38.03799896053619	40.0	37.0	41.0	33.0	41.0
36-37	38.01142289144123	40.0	37.0	41.0	33.0	41.0
38-39	37.966000677327166	40.0	37.0	41.0	33.0	41.0
40-41	37.90047799312079	40.0	37.0	41.0	33.0	41.0
42-43	37.96818064368733	40.0	37.0	41.0	33.0	41.0
44-45	37.97566030335422	40.0	37.0	41.0	33.0	41.0
46-47	37.91419733833632	40.0	37.0	41.0	33.0	41.0
48-49	37.82764059355116	40.0	37.0	41.0	33.0	41.0
50-51	37.70593835143069	40.0	37.0	41.0	32.5	41.0
52-53	37.53153815189377	40.0	36.0	41.0	32.0	41.0
54-55	37.38621536076897	39.0	36.0	41.0	32.0	41.0
56-57	37.2436524263825	39.0	36.0	41.0	31.0	41.0
58-59	37.07389773487621	39.0	35.0	41.0	31.0	41.0
60-61	36.88538673683932	39.0	35.0	40.0	31.0	41.0
62-63	36.67849347939659	38.0	35.0	40.0	31.0	41.0
64-65	36.37905495481643	38.0	35.0	40.0	30.5	41.0
66-67	36.08964172852421	37.5	34.5	40.0	30.0	41.0
68-69	35.782546311318214	37.0	34.0	39.5	30.0	41.0
70-71	35.47737832690933	36.0	34.0	39.0	29.0	41.0
72-73	35.19596053551116	36.0	34.0	39.0	29.0	40.5
74-75	34.8677232349178	35.5	34.0	38.5	29.0	40.0
76-77	33.599601289328035	34.5	32.0	36.5	28.0	39.0
78-79	34.30078012287484	35.0	33.5	37.0	29.0	39.0
80-81	34.21080399173398	35.0	34.0	37.0	29.0	39.0
82-83	33.97912504631204	35.0	33.0	36.0	29.0	38.5
84-85	33.74301079661521	35.0	33.0	36.0	29.0	37.0
86-87	33.54306779126235	35.0	33.0	35.5	29.0	37.0
88-89	33.37290689479258	35.0	33.0	35.0	29.0	36.5
90-91	33.24288313159214	35.0	33.0	35.0	29.0	36.0
92-93	33.18510706188961	35.0	33.0	35.0	29.0	36.0
94-95	33.11528184487574	35.0	33.0	35.0	29.0	36.0
96-97	33.15252827858406	35.0	33.0	35.0	29.0	35.0
98-99	33.27118321537901	35.0	33.0	35.0	29.0	35.0
100	32.05496020509089	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	32.0
21	191.0
22	959.0
23	3407.0
24	9155.0
25	19720.0
26	36276.0
27	56751.0
28	81159.0
29	108608.0
30	138255.0
31	170690.0
32	211765.0
33	267300.0
34	353452.0
35	485267.0
36	741707.0
37	1248611.0
38	1564674.0
39	863207.0
40	33754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35439788770662	20.48697838683523	14.046532065561157	24.112091659896997
2	14.847899395538194	21.281731117312187	40.83999158710513	23.03037790004449
3	18.225163653286156	25.991888564082565	30.691805154151997	25.091142628479286
4	12.606941889257845	17.911147007519222	38.628229015261276	30.853682087961666
5	12.993372108751522	37.94320357720043	35.88901859202855	13.174405722019502
6	30.196006376911765	37.98131180174341	18.498235715866205	13.324446105478623
7	26.647703772276383	32.567269929608464	22.99871226413988	17.786314033975273
8	24.645653715551894	36.77934681220871	21.09300392732072	17.481995544918682
9	25.40231385883694	16.63007265895172	21.296696062280443	36.6709174199309
10-11	24.328778433590905	26.772270910852242	28.802952957374927	20.09599769818192
12-13	24.855835351203176	24.210810257164056	29.104511453968723	21.828842937664046
14-15	21.938961163856767	25.79269250947428	26.550329987200826	25.718016339468125
16-17	21.706629536925163	29.006031159177738	27.2815951715073	22.005744132389797
18-19	21.666870611361038	28.17263338471697	28.938227432607356	21.222268571314636
20-21	22.393322221007676	27.341614564934307	28.87561122873454	21.389451985323475
22-23	22.165510262968784	27.34475739763423	28.695595396071848	21.79413694332514
24-25	22.06242281615169	27.394852037731805	28.783406577334798	21.759318568781712
26-27	22.000807047747507	27.63791897487878	28.65268331638877	21.70859066098494
28-29	21.90766482666692	27.678012283558246	28.67877099280209	21.735551896972748
30-31	22.02562299330137	27.575637449139844	28.71490341256618	21.683836144992608
32-33	21.889901306409758	27.67602838714735	28.656612904990293	21.7774574014526
34-35	22.0817812837822	27.60795107802285	28.679148824370632	21.631118813824322
36-37	21.95370944649944	27.737177633106157	28.5034567094391	21.805656210955306
38-39	21.85249340362621	27.663189667563294	28.632465195786533	21.851851733023963
40-41	22.01688334114992	27.555455058513484	28.566308496180238	21.861353104156358
42-43	21.96842872783141	27.624621925720206	28.45614207522211	21.95080727122627
44-45	22.017469170265258	27.815227697690148	28.202935437682903	21.964367694361695
46-47	22.112687855846154	27.675180150876383	27.94688050515644	22.265251488121017
48-49	22.073854213849817	27.81454934382037	27.930067245834362	22.18152919649545
50-51	21.993678272717922	27.925516309657223	27.911476973304104	22.169328444320755
52-53	22.060744693271296	27.896818773431114	27.886657182515144	22.155779350782442
54-55	22.087820865930638	27.831463802190765	27.835210083417195	22.245505248461406
56-57	22.177767326110384	27.911866314699708	27.72041719101552	22.18994916817439
58-59	22.033712779702157	28.071867884487762	27.843475091739688	22.05094424407039
60-61	22.168689694053516	28.079378072509254	27.714242334990068	22.03768989844716
62-63	22.049693299623183	28.203744940325393	27.649005624225847	22.097556135825574
64-65	22.013439207015804	28.13666681269678	27.668344534212913	22.1815494460745
66-67	22.114629249362324	28.12353499316268	27.508166594024797	22.253669163450205
68-69	22.074896314091895	28.061843301238653	27.628526226781535	22.234734157887917
70-71	22.052919851794748	27.854836174472062	27.719126755767626	22.373117217965564
72-73	22.146826956875433	27.79398917552836	27.73787878404726	22.32130508354895
74-75	22.09765313868211	27.976808044131275	27.640595571317032	22.284943245869584
76-77	22.243916860175773	27.885784160158856	27.524116415848233	22.346182563817138
78-79	22.17717469415232	27.832830258854685	27.551177202356687	22.438817844636304
80-81	22.11425871252389	27.860477212131546	27.61043604350968	22.414828031834887
82-83	22.17140203617723	27.831187582534366	27.747053517299015	22.250356863989385
84-85	22.264987004871397	27.840473158392232	27.623566814318256	22.270973022418108
86-87	22.137229907011253	27.845469712273648	27.750194882758706	22.2671054979564
88-89	22.230957960398058	27.757808053721973	27.72933561676425	22.28189836911572
90-91	22.262664695992047	27.802998114640904	27.70340942661904	22.23092776274801
92-93	22.263255721026745	27.729794241818368	27.795485296317107	22.211464740837776
94-95	22.062278888429105	27.7925561840971	27.89798245666408	22.24718247080971
96-97	22.068240756602847	27.939627723988824	27.99793245630099	21.99419906310734
98-99	23.194594494331845	29.852507428199047	28.191330328285737	18.761567749183374
100	29.58359508541894	41.23978062167992	29.17662429290114	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	918.0
1	851.0
2	1290.5
3	1928.5
4	2664.0
5	4424.0
6	5962.5
7	7069.5
8	7851.0
9	7755.5
10	7064.0
11	6317.0
12	6752.0
13	9191.5
14	14853.5
15	22448.5
16	29016.5
17	33273.0
18	34793.0
19	34153.0
20	32511.5
21	31067.5
22	31109.5
23	33661.0
24	39155.5
25	47994.5
26	59953.5
27	73299.0
28	87279.0
29	103254.5
30	118892.0
31	133423.0
32	148447.0
33	162313.0
34	175936.5
35	189858.0
36	203718.0
37	216776.0
38	227243.5
39	235351.0
40	243874.5
41	252678.5
42	258139.0
43	262847.5
44	269692.5
45	276600.5
46	280430.0
47	281208.0
48	279747.0
49	274948.0
50	268853.5
51	260601.0
52	247910.5
53	234101.5
54	220768.5
55	206260.5
56	189145.0
57	170338.0
58	151921.0
59	131656.0
60	110706.0
61	90773.0
62	72686.0
63	56768.5
64	42663.5
65	32223.0
66	24349.0
67	18689.5
68	14403.5
69	10735.5
70	7905.0
71	5698.0
72	4091.5
73	2865.0
74	1949.5
75	1332.0
76	898.5
77	589.0
78	383.5
79	253.5
80	170.0
81	107.0
82	76.5
83	57.5
84	39.5
85	25.0
86	17.0
87	14.5
88	10.0
89	7.0
90	7.5
91	6.0
92	4.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.5965907447210255E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001118070601076319
18-19	0.0037138708777010587
20-21	0.0
22-23	0.0013293317840101717
24-25	4.6925995359019056E-4
26-27	0.0138479416208335
28-29	0.007523111452430215
30-31	7.608278151746872E-4
32-33	0.0070592190567750086
34-35	0.004631530527200165
36-37	0.002123294545168837
38-39	0.0016282126420044051
40-41	0.004063974076055089
42-43	0.009051130794481606
44-45	0.010349706839593844
46-47	0.008870651424655908
48-49	0.008096354066097416
50-51	0.03551829350526425
52-53	0.013031082547454716
54-55	8.732582645598787E-5
56-57	0.008085264587635617
58-59	0.006802840730589384
60-61	1.3547947269221556E-4
62-63	0.005490357935709462
64-65	0.002347517638729974
66-67	0.024688395574357085
68-69	0.03260197933273477
70-71	0.015389241172079958
72-73	0.011261659902538988
74-75	0.016714789773619926
76-77	0.002212717724696252
78-79	0.002867129351348328
80-81	0.0018217231064800226
82-83	0.02521517729538273
84-85	9.468127914408124E-4
86-87	0.009657823835103704
88-89	7.478429607818145E-4
90-91	0.0032970917886094415
92-93	0.0011461530552563759
94-95	8.152041311330476E-4
96-97	0.002953546618779831
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	157.0
20-21	424.0
22-23	866.0
24-25	2005.0
26-27	2644.0
28-29	9415.0
30-31	20274.0
32-33	25411.0
34-35	34452.0
36-37	51299.0
38-39	57586.0
40-41	59745.0
42-43	64181.0
44-45	73270.0
46-47	72254.0
48-49	66547.0
50-51	58288.0
52-53	55652.0
54-55	59937.0
56-57	63814.0
58-59	64671.0
60-61	63349.0
62-63	62638.0
64-65	65879.0
66-67	70060.0
68-69	68820.0
70-71	64565.0
72-73	61810.0
74-75	63308.0
76-77	62454.0
78-79	66644.0
80-81	67386.0
82-83	65792.0
84-85	66724.0
86-87	70702.0
88-89	76626.0
90-91	83435.0
92-93	92468.0
94-95	106976.0
96-97	237099.0
98-99	1319644.0
100-101	2715674.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.73573549093426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.13801566791409	77.2804906898197
2	6.863378126594666	11.768735432719685
3	1.7121627583048165	4.403806000903505
4	0.615505045375781	2.110831110546938
5	0.2633224510800733	1.128807200731282
6	0.14651269895984415	0.7536824402450446
7	0.0766247531661143	0.4598635698655672
8	0.04707799568431532	0.32290132683470424
9	0.03059654897564538	0.23608958669202135
>10	0.10423951962907672	1.336826136466087
>50	0.002152861265659693	0.12006505320728894
>100	3.83093734930576E-4	0.05897831555822155
>500	2.847931503298027E-5	0.01892313640988703
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.127470212800892E-5
2	0.0	4.6912053192013376E-5	0.0	0.0	3.127470212800892E-5
3	0.0	6.254940425601783E-5	0.0	0.0	3.127470212800892E-5
4	0.0	1.2509880851203567E-4	0.0	0.0	6.254940425601783E-5
5	3.127470212800892E-5	1.4073615957604013E-4	0.0	0.0	6.254940425601783E-5
6	3.127470212800892E-5	1.4073615957604013E-4	0.0	1.563735106400446E-5	7.81867553200223E-5
7	3.127470212800892E-5	1.4073615957604013E-4	0.0	1.563735106400446E-5	9.382410638402675E-5
8	3.127470212800892E-5	1.4073615957604013E-4	0.0	3.127470212800892E-5	9.382410638402675E-5
9	3.127470212800892E-5	3.5965907447210255E-4	0.0	6.254940425601783E-5	9.382410638402675E-5
10-11	6.254940425601783E-5	3.5965907447210255E-4	0.0	6.254940425601783E-5	1.0946145744803122E-4
12-13	6.254940425601783E-5	3.5965907447210255E-4	0.0	1.0164278191602898E-4	1.6419218617204682E-4
14-15	7.036807978802006E-5	4.06571127664116E-4	0.0	1.0946145744803122E-4	2.423789414920691E-4
16-17	7.81867553200223E-5	4.06571127664116E-4	0.0	1.2509880851203567E-4	3.2838437234409363E-4
18-19	7.81867553200223E-5	4.3002715426012267E-4	0.0	2.8147231915208026E-4	3.5965907447210255E-4
20-21	7.81867553200223E-5	4.5348318085612936E-4	0.0	9.147850372442608E-4	3.75296425536107E-4
22-23	1.0164278191602898E-4	5.160325851121471E-4	0.0	0.0021188610691726044	4.222084787281204E-4
24-25	1.4073615957604013E-4	5.78581989368165E-4	0.0	0.00462865591494532	4.847578829841383E-4
26-27	1.563735106400446E-4	5.942193404321694E-4	0.0	0.009491872095850708	4.847578829841383E-4
28-29	1.563735106400446E-4	5.942193404321694E-4	0.0	0.029468588080116404	4.847578829841383E-4
30-31	1.876482127680535E-4	6.724060957521917E-4	0.0	0.03247877815993726	4.847578829841383E-4
32-33	2.03285563832058E-4	6.724060957521917E-4	0.0	0.03885881739405108	5.316699361761516E-4
34-35	2.423789414920691E-4	7.271368244762073E-4	0.0	0.04694332789414139	5.316699361761516E-4
36-37	2.8147231915208026E-4	7.896862287322252E-4	0.0	0.05903100026661684	5.316699361761516E-4
38-39	2.8147231915208026E-4	8.13142255328232E-4	0.0	0.08832757748502919	5.394886117081538E-4
40-41	2.8147231915208026E-4	9.069663617122587E-4	0.0	0.11910188437898997	5.551259627721583E-4
42-43	2.8147231915208026E-4	9.460597393722698E-4	0.0	0.1395868142728358	5.864006649001672E-4
44-45	2.8147231915208026E-4	0.0011102519255443166	0.0	0.16361360418267867	6.098566914961739E-4
46-47	2.8147231915208026E-4	0.00122753205852435	0.0	0.18878973939572585	6.176753670281761E-4
48-49	2.8147231915208026E-4	0.0012353507340563522	0.0	0.2122066726140725	6.645874202201895E-4
50-51	2.8147231915208026E-4	0.0013526308670363857	0.0	0.2345133539068749	6.724060957521917E-4
52-53	3.2838437234409363E-4	0.001360449542568388	0.0	0.28617134314681364	6.880434468161962E-4
54-55	3.2838437234409363E-4	0.001360449542568388	0.0	0.3461171284506747	6.958621223481984E-4
56-57	3.2838437234409363E-4	0.001360449542568388	0.0	0.4042724370577073	7.036807978802006E-4
58-59	3.2838437234409363E-4	0.0013760868936323924	0.0	0.44370983644112655	7.036807978802006E-4
60-61	3.440217234080981E-4	0.0014229989468244057	0.0	0.47299859498400687	7.193181489442051E-4
62-63	3.440217234080981E-4	0.0014699110000164191	0.0	0.49514108409063723	7.50592851072214E-4
64-65	3.440217234080981E-4	0.0016575592127844728	0.0	0.5036556217449877	7.584115266042162E-4
66-67	3.440217234080981E-4	0.0016575592127844728	0.0	0.5112631930376258	7.662302021362185E-4
68-69	3.440217234080981E-4	0.0016888339149124818	0.0	0.5185032865802599	7.662302021362185E-4
70-71	3.440217234080981E-4	0.0017044712659764862	0.0	0.5248598697877778	7.975049042642275E-4
72-73	3.440217234080981E-4	0.0017122899415084884	0.0	0.5300123769633671	8.13142255328232E-4
74-75	3.440217234080981E-4	0.0017748393457645063	0.0	0.5339764454580923	8.209609308602342E-4
76-77	3.440217234080981E-4	0.0018139327234245174	0.0	0.5373541132879173	8.287796063922364E-4
78-79	3.440217234080981E-4	0.0018452074255525263	0.0	0.539848270782626	8.287796063922364E-4
80-81	3.440217234080981E-4	0.0018608447766165308	0.0	0.5406535943624222	8.522356329882431E-4
82-83	3.440217234080981E-4	0.001868663452148533	0.0	0.5412009016496624	9.069663617122587E-4
84-85	3.440217234080981E-4	0.0018764821276805353	0.0	0.5414198245645584	9.147850372442609E-4
86-87	3.440217234080981E-4	0.0018764821276805353	0.0	0.5415214673464744	9.226037127762632E-4
88	3.440217234080981E-4	0.0019859435851285663	0.0	0.5415371046975385	9.226037127762632E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7255	0.0	45.73639	1
GTATCAA	13210	0.0	34.165108	1
ATCAACG	15165	0.0	28.81291	3
TCAACGC	15185	0.0	28.74792	4
CAACGCA	15630	0.0	27.929438	5
TATCAAC	15970	0.0	27.617685	2
AACGCAG	16005	0.0	27.377682	6
ACGCAGA	18615	0.0	23.53907	7
CGCAGAG	19085	0.0	22.894827	8
GTACATG	18300	0.0	21.004517	1
TACATGG	18160	0.0	20.64633	2
ACATGGG	18385	0.0	19.723545	3
GCAGAGT	22810	0.0	19.137972	9
GAGTACT	12700	0.0	18.723991	12-13
GTGGTAT	4170	0.0	18.317442	1
CATGGGG	13685	0.0	18.185102	4
CAGAGTA	21955	0.0	17.10593	10-11
TGGTATC	4045	0.0	17.056059	2
AGAGTAC	20030	0.0	16.904648	10-11
GTACTTT	14690	0.0	16.313778	14-15
>>END_MODULE