FastQCFastQC Report
Fri 27 May 2016
SRR2140285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6806947
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.041.7361451
GTATCAA132300.033.6724171
TCAACGC147750.029.2600124
ATCAACG148250.029.1617553
CAACGCA149950.028.7209975
TATCAAC158400.027.760562
AACGCAG156050.027.651016
ACGCAGA183800.023.45397
CGCAGAG188800.022.810988
TACATGG188000.022.5364272
GTACATG195100.022.454221
ACATGGG195250.021.2150523
GCAGAGT221350.019.2707449
GTGGTAT42350.019.134561
CATGGGG153850.018.9560574
GAGTACT120700.017.82483912-13
TGGTATC44200.016.8446482
AGAGTAC197750.016.83847810-11
CAGAGTA217700.016.60859510-11
CTTATAC81250.016.605661
Produced by FastQC (version 0.11.5)