##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6806947
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.061105514704316	33.0	31.0	34.0	30.0	34.0
2	32.195021791707795	33.0	31.0	34.0	30.0	34.0
3	32.10143166973388	33.0	31.0	34.0	30.0	34.0
4	35.9841088817057	37.0	35.0	37.0	35.0	37.0
5	35.85363585172618	37.0	35.0	37.0	35.0	37.0
6	35.9086756515072	37.0	35.0	37.0	35.0	37.0
7	35.85890840636779	37.0	35.0	37.0	35.0	37.0
8	35.85289146514583	37.0	35.0	37.0	35.0	37.0
9	37.60954419066286	39.0	37.0	39.0	35.0	39.0
10-11	37.48981349494862	39.0	37.0	39.0	34.5	39.0
12-13	37.46250874290633	39.0	37.0	39.0	34.0	39.0
14-15	38.7620007177961	40.0	38.0	41.0	34.5	41.0
16-17	38.60917794717661	40.0	38.0	41.0	34.0	41.0
18-19	38.67684117417103	40.0	38.0	41.0	34.0	41.0
20-21	38.70244375003212	40.0	38.0	41.0	34.0	41.0
22-23	38.62848490261075	40.0	38.0	41.0	34.0	41.0
24-25	38.573389896558105	40.0	38.0	41.0	34.0	41.0
26-27	38.36818031195576	40.0	38.0	41.0	34.0	41.0
28-29	38.25454763321719	40.0	38.0	41.0	34.0	41.0
30-31	38.13376291532533	40.0	38.0	41.0	33.0	41.0
32-33	37.95634195610124	40.0	37.0	41.0	33.0	41.0
34-35	38.04516566697254	40.0	37.0	41.0	33.0	41.0
36-37	38.01735122987246	40.0	37.0	41.0	33.0	41.0
38-39	37.965678973393146	40.0	37.0	41.0	33.0	41.0
40-41	37.90046515811257	40.0	37.0	41.0	33.0	41.0
42-43	37.97053036636051	40.0	37.0	41.0	33.0	41.0
44-45	37.974933409959206	40.0	37.0	41.0	33.0	41.0
46-47	37.91178692318478	40.0	37.0	41.0	33.0	41.0
48-49	37.82197750409507	40.0	37.0	41.0	33.0	41.0
50-51	37.69040576459766	40.0	37.0	41.0	32.0	41.0
52-53	37.51386291533349	39.5	36.0	41.0	32.0	41.0
54-55	37.36212628878352	39.0	36.0	41.0	31.5	41.0
56-57	37.2113022185809	39.0	35.5	41.0	31.0	41.0
58-59	37.03091574116477	39.0	35.0	41.0	31.0	41.0
60-61	36.83893638641881	39.0	35.0	40.0	31.0	41.0
62-63	36.62272232548822	38.0	35.0	40.0	31.0	41.0
64-65	36.3265350433946	38.0	35.0	40.0	30.5	41.0
66-67	36.03342864660232	37.0	34.5	40.0	30.0	41.0
68-69	35.722895788539034	37.0	34.0	39.5	30.0	41.0
70-71	35.41831733240107	36.0	34.0	39.0	29.0	41.0
72-73	35.13230547275522	36.0	34.0	39.0	29.0	40.0
74-75	34.8053998205529	35.0	34.0	38.5	29.0	40.0
76-77	33.545327162281666	34.5	32.0	36.5	28.0	39.0
78-79	34.24376158911235	35.0	33.0	37.0	29.0	39.0
80-81	34.15839406251324	35.0	33.5	36.5	29.0	39.0
82-83	33.93575930793861	35.0	33.0	36.0	29.0	38.0
84-85	33.70571226984508	35.0	33.0	36.0	29.0	37.0
86-87	33.51214996939938	35.0	33.0	35.0	29.0	37.0
88-89	33.35093481968208	35.0	33.0	35.0	29.0	36.5
90-91	33.22662777299241	35.0	33.0	35.0	29.0	36.0
92-93	33.17808071190001	35.0	33.0	35.0	29.0	36.0
94-95	33.1115885428246	35.0	33.0	35.0	29.0	36.0
96-97	33.150971263028154	35.0	33.0	35.0	29.0	35.0
98-99	33.271616978269805	35.0	33.0	35.0	29.0	35.0
100	32.080482727706006	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	37.0
21	226.0
22	1068.0
23	3785.0
24	10083.0
25	21515.0
26	38268.0
27	60808.0
28	87490.0
29	115704.0
30	147076.0
31	181820.0
32	224655.0
33	284882.0
34	379138.0
35	520029.0
36	799810.0
37	1338888.0
38	1656685.0
39	900172.0
40	34800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.585324522138926	20.26000790075198	13.848822386893861	24.30584519021523
2	14.99077339664904	20.97792152634654	40.67162562012015	23.35967945688427
3	18.529378926151622	25.533016400956676	30.407717440487037	25.52988723240467
4	12.776697100770729	17.624362287527727	38.12976654585382	31.469174065847728
5	13.277641209781713	37.76939940916243	35.63903171274875	13.313927668307098
6	30.8283287647164	37.44921181257912	18.24418494811257	13.478274474591915
7	27.160913695963846	32.29199522194017	22.535110086798092	18.01198099529789
8	24.96008856834055	36.60724844779899	20.808344768954424	17.624318214906037
9	25.480468703517158	16.575566109152902	20.971501614453587	36.97246357287636
10-11	24.703512455730888	26.53733751709834	28.46712336676046	20.292026660410315
12-13	25.16339557219999	23.879868610700218	28.76686861231621	22.189867204783585
14-15	22.190902911393316	25.46224467444803	26.165357244591448	26.18149516956721
16-17	22.08315217475139	28.723175216139722	26.828901017301387	22.364771591807497
18-19	22.077214216517493	27.9367426734408	28.428054582899076	21.557988527142623
20-21	22.76969800299662	27.11681410897702	28.300498033290417	21.812989854735946
22-23	22.543653938395828	27.15109192608487	28.15459088246623	22.150663253053075
24-25	22.43881473253635	27.23883654209962	28.24912341060419	22.073225314759835
26-27	22.440786720438506	27.40860861108146	28.170403256425253	21.980201412054782
28-29	22.27320070963899	27.441583985326908	28.137795104103148	22.14742020093096
30-31	22.426953033606523	27.35298943726809	28.23445057267442	21.985606956450972
32-33	22.318516063099533	27.46671613004668	28.1079650685587	22.106802738295084
34-35	22.452131761529788	27.373516334137772	28.241681430545608	21.932670473786835
36-37	22.299636599455496	27.451967473260474	28.081612709455882	22.166783217828144
38-39	22.219025840211877	27.358021993753745	28.185677311924774	22.237274854109607
40-41	22.46317613095365	27.22264979755238	28.113685520203695	22.20048855129028
42-43	22.396771007877426	27.318741639161974	27.944650676315963	22.339836676644637
44-45	22.347908031777298	27.467573539781814	27.89605835726633	22.288460071174555
46-47	22.48080367890704	27.30651392609588	27.587930363433067	22.62475203156401
48-49	22.391872407366826	27.500334123204297	27.593817107711942	22.513976361716935
50-51	22.29658461994324	27.597529374749524	27.61186283202763	22.494023173279608
52-53	22.408708593192685	27.570727629977267	27.559906297317983	22.46065747951207
54-55	22.425049139275202	27.460771116944553	27.53066755274244	22.583512191037805
56-57	22.533057522001492	27.630799322243565	27.353508221084827	22.482634934670116
58-59	22.288897311593374	27.81045764775982	27.589520837480453	22.311124203166354
60-61	22.514570189887777	27.850891375978414	27.35566130823636	22.27887712589744
62-63	22.410451324501267	27.962994025286026	27.231676411851186	22.394878238361517
64-65	22.339024140065803	27.93632627870461	27.29281508097628	22.43183450025331
66-67	22.44733107105872	27.844846672189583	27.150768463514357	22.55705379323734
68-69	22.374852562614382	27.769686205118767	27.299160982687166	22.55630024957969
70-71	22.31415462413984	27.56469421461344	27.3688701046342	22.75228105661252
72-73	22.45840266603068	27.57409118197743	27.3045731710103	22.662932980981594
74-75	22.3773702133429	27.697287455005405	27.271861286653355	22.653481044998344
76-77	22.46292405357752	27.64717693903046	27.196213608001525	22.693685399390496
78-79	22.391911878258018	27.663105907839924	27.177696120371543	22.76728609353052
80-81	22.406934193477717	27.710831218283253	27.19881855056255	22.683416037676476
82-83	22.418653998663313	27.697721591008733	27.354245541290194	22.52937886903776
84-85	22.575289301610738	27.66639346835556	27.232557051290527	22.52576017874317
86-87	22.450068364523343	27.651289363421398	27.321587618987063	22.577054653068192
88-89	22.45061762220205	27.68365470413679	27.308586830911825	22.55714084274934
90-91	22.50563100485903	27.73438505375076	27.296670102136932	22.463313839253274
92-93	22.574971409264084	27.656338564837156	27.32869109953155	22.43999892636721
94-95	22.29093243375259	27.799832752610243	27.46621924067192	22.443015572965248
96-97	22.321299008113822	27.86469071024713	27.54625410336498	22.267756178274066
98-99	23.421540901204835	29.868473280477136	27.704442864923244	19.005542953394787
100	29.995876810289378	41.359636495724224	28.644486693986398	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1061.0
1	988.0
2	1390.5
3	1994.5
4	2711.5
5	4437.5
6	5951.5
7	7010.0
8	7748.5
9	7655.0
10	6994.5
11	6255.5
12	6440.0
13	8276.0
14	13096.0
15	19881.5
16	25849.0
17	29836.0
18	31374.0
19	31009.0
20	29622.0
21	28008.5
22	27742.5
23	30100.0
24	35435.0
25	43835.0
26	55037.5
27	67676.5
28	81571.0
29	97854.0
30	113616.0
31	128621.5
32	144640.0
33	160694.5
34	176614.0
35	191801.5
36	207726.0
37	222406.5
38	233653.0
39	242303.5
40	251903.0
41	261956.5
42	269465.5
43	277000.5
44	285214.5
45	294820.5
46	302669.5
47	307714.5
48	306835.5
49	301364.0
50	297369.5
51	290655.5
52	279992.0
53	266957.0
54	252136.0
55	235172.5
56	215568.0
57	194942.5
58	174382.5
59	151446.5
60	127636.0
61	105238.5
62	84997.5
63	67160.0
64	51425.0
65	39698.5
66	30664.5
67	23585.5
68	18114.0
69	13387.5
70	9673.5
71	7120.5
72	5272.5
73	3704.0
74	2507.5
75	1696.0
76	1164.5
77	762.5
78	496.5
79	355.0
80	242.0
81	152.5
82	109.0
83	80.5
84	55.0
85	36.5
86	25.5
87	20.0
88	18.5
89	14.5
90	10.0
91	7.0
92	4.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.9665359521676895E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.622522402480878E-4
18-19	0.004010608573858441
20-21	0.0
22-23	0.001476641080860131
24-25	5.878553925701985E-4
26-27	0.013497014777907946
28-29	0.0074454751375573605
30-31	8.108442008570181E-4
32-33	0.00687765843684579
34-35	0.004864119882806644
36-37	0.0020993710777341824
38-39	0.001574724956203433
40-41	0.004106582547632556
42-43	0.008523296626847771
44-45	0.010252344263320558
46-47	0.008859508691547172
48-49	0.008326562704340443
50-51	0.03512230602154388
52-53	0.013309927543220156
54-55	1.0649937853516495E-4
56-57	0.008712210991862033
58-59	0.006601445280888146
60-61	1.1866859599200207E-4
62-63	0.005728097829050923
64-65	0.0025425660704884354
66-67	0.024911990526059663
68-69	0.0335512142893606
70-71	0.01556854428425039
72-73	0.01179544703053961
74-75	0.017004906178967067
76-77	0.002405753889317723
78-79	0.002824991314099693
80-81	0.0017775232663383214
82-83	0.025373084173867477
84-85	8.983674885741971E-4
86-87	0.009901987345880924
88-89	8.234691555903288E-4
90-91	0.0031731699078478386
92-93	0.0012104536885681595
94-95	7.845369276158288E-4
96-97	0.002753934614956254
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	179.0
20-21	553.0
22-23	1155.0
24-25	2722.0
26-27	4262.0
28-29	10061.0
30-31	20972.0
32-33	26172.0
34-35	35909.0
36-37	53629.0
38-39	61344.0
40-41	62489.0
42-43	66472.0
44-45	75963.0
46-47	74401.0
48-49	69152.0
50-51	62211.0
52-53	60200.0
54-55	64355.0
56-57	68951.0
58-59	69615.0
60-61	68440.0
62-63	68360.0
64-65	72031.0
66-67	76078.0
68-69	73515.0
70-71	68961.0
72-73	65218.0
74-75	66195.0
76-77	65667.0
78-79	69808.0
80-81	71299.0
82-83	68688.0
84-85	69684.0
86-87	74601.0
88-89	79039.0
90-91	85300.0
92-93	94522.0
94-95	114845.0
96-97	254847.0
98-99	1415691.0
100-101	2893391.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.65899210263723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.98452197063045	77.07983456841822
2	7.027339000741203	12.03909551934091
3	1.7166077400271806	4.411286665389428
4	0.6164195650429047	2.1120751461568505
5	0.25909997299802856	1.1097121270415824
6	0.1376947336809059	0.7076875262968473
7	0.0788134002304935	0.4725753499548081
8	0.0474898221751483	0.32543442421253393
9	0.03212406193111831	0.24765432905360635
>10	0.09749723288870497	1.2470616113659587
>50	0.0016519294499937605	0.0923009986855641
>100	6.606460843571789E-4	0.10829385227692384
>500	7.99241195002009E-5	0.04698788180675491
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.938174779383474E-5	0.0	0.0	0.0
3	0.0	2.938174779383474E-5	0.0	0.0	0.0
4	0.0	4.407262169075211E-5	0.0	0.0	0.0
5	0.0	5.876349558766948E-5	0.0	0.0	0.0
6	1.469087389691737E-5	5.876349558766948E-5	0.0	0.0	0.0
7	1.469087389691737E-5	5.876349558766948E-5	0.0	1.469087389691737E-5	0.0
8	1.469087389691737E-5	7.345436948458685E-5	0.0	1.469087389691737E-5	0.0
9	1.469087389691737E-5	1.3221786507225633E-4	0.0	2.938174779383474E-5	0.0
10-11	2.938174779383474E-5	1.3221786507225633E-4	0.0	4.407262169075211E-5	0.0
12-13	2.938174779383474E-5	1.3221786507225633E-4	0.0	4.407262169075211E-5	0.0
14-15	2.938174779383474E-5	1.6894504981454977E-4	0.0	4.407262169075211E-5	0.0
16-17	3.6727184742293426E-5	1.909813606599258E-4	0.0	5.876349558766948E-5	0.0
18-19	4.407262169075211E-5	2.2036310845376054E-4	0.0	7.345436948458685E-5	0.0
20-21	4.407262169075211E-5	2.497448562475953E-4	0.0	1.1752699117533896E-4	0.0
22-23	4.407262169075211E-5	2.938174779383474E-4	0.0	1.8363592371146712E-4	2.2036310845376054E-5
24-25	4.407262169075211E-5	3.378900996290995E-4	0.0	2.717811670929713E-4	4.407262169075211E-5
26-27	5.14180586392108E-5	3.5258097352601687E-4	0.0	4.407262169075211E-4	5.876349558766948E-5
28-29	5.876349558766948E-5	3.599264104744756E-4	0.0	0.0012707605920833525	5.876349558766948E-5
30-31	7.345436948458685E-5	3.96653595216769E-4	0.0	0.004253007993157578	5.876349558766948E-5
32-33	7.345436948458685E-5	4.260353430106037E-4	0.0	0.009571104343841667	5.876349558766948E-5
34-35	9.54906803299629E-5	4.994897124951905E-4	0.0	0.016615378377413545	5.876349558766948E-5
36-37	1.1752699117533896E-4	5.655986450313188E-4	0.0	0.028110987201751387	5.876349558766948E-5
38-39	1.1752699117533896E-4	5.729440819797774E-4	0.0	0.0579775338341844	7.345436948458685E-5
40-41	1.1752699117533896E-4	6.096712667220709E-4	0.0	0.08908545931090693	7.345436948458685E-5
42-43	1.1752699117533896E-4	6.390530145159056E-4	0.0	0.10907973868461147	7.345436948458685E-5
44-45	1.1752699117533896E-4	6.97816510103575E-4	0.0	0.13272470022169996	7.345436948458685E-5
46-47	1.2487242812379764E-4	7.345436948458685E-4	0.0	0.1583455842979239	7.345436948458685E-5
48-49	1.3221786507225633E-4	7.418891317943271E-4	0.0	0.18124865670321805	7.345436948458685E-5
50-51	1.3221786507225633E-4	8.079980643304553E-4	0.0	0.20432801959527525	7.345436948458685E-5
52-53	1.3221786507225633E-4	8.079980643304553E-4	0.0	0.2557020056127953	8.814524338150422E-5
54-55	1.3221786507225633E-4	8.15343501278914E-4	0.0	0.31470790061976395	1.0283611727842158E-4
56-57	1.3221786507225633E-4	8.373798121242901E-4	0.0	0.3725238348410822	1.0283611727842158E-4
58-59	1.3221786507225633E-4	8.373798121242901E-4	0.0	0.41239486659731595	1.0283611727842158E-4
60-61	1.469087389691737E-4	8.520706860212074E-4	0.0	0.4412624338047586	1.0283611727842158E-4
62-63	1.469087389691737E-4	8.520706860212074E-4	0.0	0.46468703223339336	1.0283611727842158E-4
64-65	1.469087389691737E-4	9.402159294027117E-4	0.0	0.4725833769529864	1.0283611727842158E-4
66-67	1.469087389691737E-4	9.54906803299629E-4	0.0	0.48022997681633195	1.0283611727842158E-4
68-69	1.469087389691737E-4	9.695976771965464E-4	0.0	0.4872522145390584	1.0283611727842158E-4
70-71	1.469087389691737E-4	9.916339880419226E-4	0.0	0.4935986720625267	1.0283611727842158E-4
72-73	1.469087389691737E-4	9.98979424990381E-4	0.0	0.4989241138501593	1.1752699117533896E-4
74-75	1.469087389691737E-4	0.0010136702988872985	0.0	0.5030375585412961	1.1752699117533896E-4
76-77	1.469087389691737E-4	0.001050397483629592	0.0	0.5065560228396078	1.1752699117533896E-4
78-79	1.469087389691737E-4	0.001072433794474968	0.0	0.5086127451851763	1.1752699117533896E-4
80-81	1.469087389691737E-4	0.001072433794474968	0.0	0.509376670627816	1.3221786507225633E-4
82-83	1.469087389691737E-4	0.0010871246683718855	0.0	0.5100891780118164	1.469087389691737E-4
84-85	1.469087389691737E-4	0.0011018155422688027	0.0	0.510258123061631	1.469087389691737E-4
86-87	1.469087389691737E-4	0.0011458881639595548	0.0	0.510338922868064	1.469087389691737E-4
88	1.469087389691737E-4	0.0012046516595472243	0.0	0.5103462683050125	1.469087389691737E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7520	0.0	41.736145	1
GTATCAA	13230	0.0	33.672417	1
TCAACGC	14775	0.0	29.260012	4
ATCAACG	14825	0.0	29.161755	3
CAACGCA	14995	0.0	28.720997	5
TATCAAC	15840	0.0	27.76056	2
AACGCAG	15605	0.0	27.65101	6
ACGCAGA	18380	0.0	23.4539	7
CGCAGAG	18880	0.0	22.81098	8
TACATGG	18800	0.0	22.536427	2
GTACATG	19510	0.0	22.45422	1
ACATGGG	19525	0.0	21.215052	3
GCAGAGT	22135	0.0	19.270744	9
GTGGTAT	4235	0.0	19.13456	1
CATGGGG	15385	0.0	18.956057	4
GAGTACT	12070	0.0	17.824839	12-13
TGGTATC	4420	0.0	16.844648	2
AGAGTAC	19775	0.0	16.838478	10-11
CAGAGTA	21770	0.0	16.608595	10-11
CTTATAC	8125	0.0	16.60566	1
>>END_MODULE