FastQCFastQC Report
Fri 27 May 2016
SRR2140284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6384969
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64000.042.730291
GTATCAA113750.033.296791
ATCAACG129050.028.3294373
TCAACGC129050.028.2975714
CAACGCA131500.027.6765335
AACGCAG137400.026.6377456
TATCAAC140150.026.5552042
ACGCAGA160700.022.698757
CGCAGAG165350.022.035548
TACATGG165700.020.623992
GTACATG167500.020.5987721
ACATGGG172250.019.2428763
GCAGAGT197800.018.2333939
CATGGGG131050.017.2277874
GAGTACT109050.017.21630312-13
GTGGTAT39800.017.048851
AGAGTAC173150.016.77975310-11
TAGACCG4251.8189894E-1216.4495545
CAGAGTA191650.016.21143710-11
ACCGAAC7650.016.1270168
Produced by FastQC (version 0.11.5)