##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6384969
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.064547690051434	33.0	31.0	34.0	30.0	34.0
2	32.1964578058249	33.0	31.0	34.0	30.0	34.0
3	32.10468053956096	33.0	31.0	34.0	30.0	34.0
4	35.98259004859695	37.0	35.0	37.0	35.0	37.0
5	35.85621621655485	37.0	35.0	37.0	35.0	37.0
6	35.91074349773664	37.0	35.0	37.0	35.0	37.0
7	35.86489801281729	37.0	35.0	37.0	35.0	37.0
8	35.857327263452646	37.0	35.0	37.0	35.0	37.0
9	37.616021001824755	39.0	37.0	39.0	35.0	39.0
10-11	37.497217762529466	39.0	37.0	39.0	35.0	39.0
12-13	37.47153760652557	39.0	37.0	39.0	34.0	39.0
14-15	38.772269215402616	40.0	38.0	41.0	35.0	41.0
16-17	38.62507092830052	40.0	38.0	41.0	34.0	41.0
18-19	38.69187595115967	40.0	38.0	41.0	34.0	41.0
20-21	38.71800797484825	40.0	38.0	41.0	34.0	41.0
22-23	38.64504329517679	40.0	38.0	41.0	34.0	41.0
24-25	38.59271051591216	40.0	38.0	41.0	34.0	41.0
26-27	38.39277581446724	40.0	38.0	41.0	34.0	41.0
28-29	38.285580247837224	40.0	38.0	41.0	34.0	41.0
30-31	38.166186292002706	40.0	38.0	41.0	33.5	41.0
32-33	37.98883478624714	40.0	37.0	41.0	33.0	41.0
34-35	38.07812322240312	40.0	37.5	41.0	33.0	41.0
36-37	38.055953063566335	40.0	37.0	41.0	33.0	41.0
38-39	38.01296202679265	40.0	37.0	41.0	33.0	41.0
40-41	37.94375969503059	40.0	37.0	41.0	33.0	41.0
42-43	38.006484922755774	40.0	37.0	41.0	33.0	41.0
44-45	38.01287463704885	40.0	37.0	41.0	33.0	41.0
46-47	37.95393155088213	40.0	37.0	41.0	33.0	41.0
48-49	37.86615932211794	40.0	37.0	41.0	33.0	41.0
50-51	37.749433891589945	40.0	37.0	41.0	33.0	41.0
52-53	37.57544282887916	40.0	36.5	41.0	32.0	41.0
54-55	37.42688162504159	39.0	36.0	41.0	32.0	41.0
56-57	37.29328597814045	39.0	36.0	41.0	31.0	41.0
58-59	37.123943894348784	39.0	35.0	41.0	31.0	41.0
60-61	36.93835096307866	39.0	35.0	40.5	31.0	41.0
62-63	36.725628524927274	38.5	35.0	40.0	31.0	41.0
64-65	36.42896578867135	38.0	35.0	40.0	30.5	41.0
66-67	36.136293471355145	37.5	34.5	40.0	30.0	41.0
68-69	35.82325560745839	37.0	34.0	39.5	30.0	41.0
70-71	35.51187794433402	36.5	34.0	39.0	29.5	41.0
72-73	35.222332045538	36.0	34.0	39.0	29.0	40.5
74-75	34.88903404777682	35.5	34.0	38.5	29.0	40.0
76-77	33.61277339872592	34.5	32.0	36.5	28.0	39.0
78-79	34.304772657290215	35.0	33.5	37.0	29.0	39.0
80-81	34.21280217482011	35.0	34.0	37.0	29.0	39.0
82-83	33.97912304651801	35.0	33.5	36.0	29.0	38.5
84-85	33.74277641647211	35.0	33.0	36.0	29.0	37.0
86-87	33.54034003438906	35.0	33.0	35.5	29.0	37.0
88-89	33.37703362699658	35.0	33.0	35.0	29.0	36.5
90-91	33.248934332882236	35.0	33.0	35.0	29.0	36.0
92-93	33.19617968331339	35.0	33.0	35.0	29.0	36.0
94-95	33.1275120293525	35.0	33.0	35.0	29.0	36.0
96-97	33.163228253781405	35.0	33.0	35.0	29.0	35.0
98-99	33.28250697393623	35.0	33.0	35.0	29.0	35.0
100	32.087778538688646	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	13.0
20	43.0
21	205.0
22	968.0
23	3454.0
24	9105.0
25	19355.0
26	35507.0
27	56323.0
28	80366.0
29	106834.0
30	135599.0
31	168272.0
32	209541.0
33	264264.0
34	349021.0
35	478432.0
36	731216.0
37	1241555.0
38	1586199.0
39	874376.0
40	34320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.725731166431665	20.228226636652423	13.722274924122576	24.323767272793337
2	15.00666643800463	21.171708116358907	40.60912433560758	23.21250111002888
3	18.455107230317303	25.51803084379074	30.46422617693989	25.56263574895207
4	12.696005258600316	17.55302179227495	38.60535579734217	31.145617151782567
5	13.177777370571414	37.8570514594511	35.78878769810785	13.176383471869638
6	30.76157456676767	37.64040201291502	18.405711914967792	13.192311505349515
7	27.127320430216656	32.391355384810794	22.832060735142175	17.649263449830375
8	24.844897445860738	36.74368661774239	20.910814132378714	17.500601804018157
9	25.579967576976493	16.60656457376692	21.114025768958314	36.699442080298276
10-11	24.556250782110297	26.663841280983508	28.764430962781496	20.0154769741247
12-13	24.989620153206697	24.01755591922216	29.03028973202532	21.96253419554582
14-15	21.989636911314683	25.65836263261419	26.467826233768715	25.88417422230241
16-17	21.902281580167763	28.775182198922867	27.176899871995204	22.14563634891417
18-19	21.813635604367825	28.129310330650448	28.731629910629785	21.32542415435194
20-21	22.58432935592558	27.312126687307586	28.587811414989016	21.51573254177782
22-23	22.308382551863605	27.266114178531627	28.40753295509254	22.017970314512223
24-25	22.112601304865024	27.3489195910321	28.514422491276157	22.02405661282672
26-27	22.136169511673316	27.516332435897507	28.359468548896512	21.98802950353267
28-29	21.99161339901444	27.67445762377233	28.382170719155077	21.951758258058156
30-31	22.30706577151885	27.47427733525491	28.326524903059923	21.892131990166313
32-33	22.072897650712	27.595753691663955	28.27789990385134	22.05344875377271
34-35	22.180196837750117	27.66392769449089	28.33244715104069	21.823428316718303
36-37	22.18684389805532	27.595045070333928	28.234028888493377	21.984082143117373
38-39	22.03186753774675	27.72819673637299	28.27785000371035	21.962085722169913
40-41	22.267119707138956	27.543632922111854	28.18056403198347	22.00868333876572
42-43	22.16272679041098	27.640932827984123	28.03210756192031	22.16423281968459
44-45	22.214075827819418	27.773443973172498	27.890460834140598	22.12201936486749
46-47	22.23652138761148	27.707258331708278	27.720269920981806	22.335950359698437
48-49	22.171801465857605	27.782934210739203	27.764467620485817	22.280796702917375
50-51	22.17153015002731	27.846567362367885	27.69116434468801	22.290738142916805
52-53	22.260237564169273	27.82277538235565	27.54198548560173	22.375001567873344
54-55	22.248444168714116	27.70847248212679	27.644030903305485	22.399052445853613
56-57	22.25007295254972	27.812072466052236	27.578050398844063	22.359804182553987
58-59	22.20286632029017	27.900355566856998	27.610573578339707	22.286204534513125
60-61	22.317211311508537	27.84017888598583	27.62517585862619	22.217433943879453
62-63	22.250018509387658	27.91333425416206	27.553864143924617	22.28278309252567
64-65	22.18012111896836	27.892372259963544	27.517312271969452	22.410194349098642
66-67	22.268501627592972	27.95345136435205	27.379382800596307	22.39866420745867
68-69	22.205241013603075	27.931738001938566	27.47178794429457	22.39123304016379
70-71	22.154897688153422	27.714137474937377	27.521082693762505	22.609882143146695
72-73	22.26863716736886	27.728253302965694	27.522985536563656	22.48012399310179
74-75	22.19603521741287	27.90087699784765	27.47806768779289	22.42502009694659
76-77	22.321112704857317	27.793718554142306	27.344107791244742	22.541060949755632
78-79	22.23978417232856	27.823315971477324	27.353836800970765	22.583063055223352
80-81	22.251914336406944	27.81695807166243	27.40364193617953	22.527485655751097
82-83	22.28890533442114	27.85317039414804	27.498151948847354	22.359772322583467
84-85	22.460825947275964	27.844163505712288	27.38567947266516	22.30933107434659
86-87	22.262742983033846	27.83911143717966	27.503004095792456	22.39514148399404
88-89	22.289529190484078	27.83347007126086	27.48182582110873	22.395174917146328
90-91	22.358887787925674	27.90367379487505	27.445143570135972	22.292294847063307
92-93	22.362211744350585	27.828986141597316	27.558244738222083	22.250557375830017
94-95	22.131828323146337	27.947812688915057	27.645342702281024	22.275016285657586
96-97	22.14774796583512	28.093269341491734	27.695043285389687	22.063939407283456
98-99	23.235309485281032	30.022772703381655	27.92307461500577	18.818843196331535
100	29.680838713185427	41.483068345307885	28.836092941506692	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1098.0
1	974.5
2	1321.0
3	1886.0
4	2486.0
5	4049.0
6	5472.0
7	6350.0
8	6884.0
9	6779.5
10	6165.5
11	5497.0
12	5817.5
13	7651.0
14	11919.5
15	17822.0
16	23056.0
17	26719.5
18	28429.0
19	28237.0
20	27102.5
21	26130.5
22	26295.0
23	28466.0
24	33290.5
25	41466.0
26	52933.5
27	65794.5
28	79249.0
29	94512.5
30	109518.0
31	124508.5
32	141010.0
33	156893.5
34	172549.5
35	188128.0
36	203803.5
37	217738.0
38	227829.0
39	236957.5
40	247163.5
41	255871.5
42	262063.0
43	268962.0
44	276085.5
45	280984.5
46	283690.0
47	286130.0
48	286090.0
49	281636.5
50	276422.0
51	268068.5
52	255248.0
53	241898.0
54	227235.0
55	210829.5
56	193415.0
57	174385.0
58	154751.5
59	132875.0
60	110266.5
61	90225.0
62	72326.5
63	56617.5
64	43575.0
65	33714.0
66	26017.0
67	20065.5
68	15394.5
69	11479.5
70	8448.0
71	6070.5
72	4280.5
73	2987.5
74	2017.5
75	1368.5
76	931.5
77	627.5
78	446.5
79	322.0
80	224.5
81	152.5
82	99.0
83	66.0
84	40.0
85	23.5
86	18.0
87	14.0
88	9.5
89	6.5
90	6.0
91	6.0
92	5.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.2889744648721084E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.144127246349983E-4
18-19	0.003688349935606579
20-21	0.0
22-23	0.0014332437360199996
24-25	4.93522787592179E-4
26-27	0.014090910195212398
28-29	0.008000195142014836
30-31	6.915122295902327E-4
32-33	0.00705534440762264
34-35	0.004701809127921266
36-37	0.0019907874320793098
38-39	0.0014616301995285842
40-41	0.0038583202122076114
42-43	0.008487064183729798
44-45	0.009960001988691427
46-47	0.00914244302989604
48-49	0.007801102216969912
50-51	0.035428583956238116
52-53	0.013414870838406074
54-55	6.988372919845285E-5
56-57	0.008334216931014668
58-59	0.0066869668338051256
60-61	1.5348951644030725E-4
62-63	0.005175970206859885
64-65	0.0022249765154190413
66-67	0.024133314559863187
68-69	0.03272456559061597
70-71	0.0150626162154621
72-73	0.011039794384314645
74-75	0.016693515403941338
76-77	0.002346483784106788
78-79	0.002779753484209489
80-81	0.0017868726532618544
82-83	0.025448886046970074
84-85	9.76225446355467E-4
86-87	0.010210487224433962
88-89	8.541542440383548E-4
90-91	0.0028809193959437756
92-93	0.0010528037058690446
94-95	7.435012273489492E-4
96-97	0.0029787768624154774
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	164.0
20-21	471.0
22-23	1066.0
24-25	2515.0
26-27	3979.0
28-29	9375.0
30-31	19244.0
32-33	24067.0
34-35	33473.0
36-37	50352.0
38-39	57551.0
40-41	58538.0
42-43	62495.0
44-45	71351.0
46-47	70595.0
48-49	65990.0
50-51	58981.0
52-53	56351.0
54-55	59492.0
56-57	62609.0
58-59	62854.0
60-61	61143.0
62-63	60989.0
64-65	63259.0
66-67	66348.0
68-69	67051.0
70-71	65076.0
72-73	62259.0
74-75	63164.0
76-77	63095.0
78-79	66969.0
80-81	68312.0
82-83	66177.0
84-85	66055.0
86-87	70567.0
88-89	75721.0
90-91	81300.0
92-93	89651.0
94-95	109159.0
96-97	239802.0
98-99	1325475.0
100-101	2721884.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.84074421790018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.18081352974207	77.4118814756874
2	6.832016193262917	11.729307090768684
3	1.7328003414797053	4.462346126809483
4	0.6008289859753743	2.0630242921524973
5	0.24640764690651226	1.0575907895718288
6	0.13807390035680805	0.7111419818217958
7	0.08357734907908879	0.5022039291295751
8	0.051381669905302234	0.3528512627063702
9	0.03236728158755698	0.2500588385807605
>10	0.09985447095169925	1.2811745004211659
>50	0.0012677761351731554	0.07314441957246308
>100	5.827480341458485E-4	0.09137819806129598
>500	2.8106583615492222E-5	0.013897094716664593
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1323566332115316E-5	0.0	0.0	0.0
3	0.0	3.1323566332115316E-5	0.0	0.0	0.0
4	0.0	7.83089158302883E-5	0.0	0.0	0.0
5	0.0	7.83089158302883E-5	0.0	0.0	0.0
6	0.0	7.83089158302883E-5	0.0	0.0	0.0
7	0.0	7.83089158302883E-5	0.0	0.0	0.0
8	0.0	7.83089158302883E-5	0.0	0.0	0.0
9	0.0	1.7227961482663425E-4	0.0	0.0	0.0
10-11	0.0	1.7227961482663425E-4	0.0	1.5661783166057658E-5	0.0
12-13	0.0	1.7227961482663425E-4	0.0	2.3492674749086487E-5	0.0
14-15	0.0	2.5841942223995136E-4	0.0	3.1323566332115316E-5	0.0
16-17	0.0	2.662503138229802E-4	0.0	4.6985349498172975E-5	0.0
18-19	0.0	2.74081205406009E-4	0.0	7.83089158302883E-5	0.0
20-21	0.0	2.8191209698903783E-4	0.0	1.4095604849451892E-4	0.0
22-23	0.0	3.523901212362973E-4	0.0	1.8011050640966307E-4	0.0
24-25	0.0	3.758827959853838E-4	0.0	2.1926496432480723E-4	0.0
26-27	0.0	3.9154457915144145E-4	0.0	3.602210128193261E-4	0.0
28-29	0.0	3.993754707344703E-4	0.0	0.0013469133522809587	0.0
30-31	0.0	4.6985349498172976E-4	0.0	0.004494931768658548	0.0
32-33	0.0	4.855152781477874E-4	0.0	0.010344607781181084	0.0
34-35	0.0	6.656257845574505E-4	0.0	0.017494211796486404	0.0
36-37	0.0	8.22243616218027E-4	0.0	0.028989960640372722	0.0
38-39	0.0	8.535671825501423E-4	0.0	0.05998462952600083	0.0
40-41	0.0	8.770598572992289E-4	0.0	0.09259246207773288	0.0
42-43	0.0	9.005525320483153E-4	0.0	0.11382984005090706	0.0
44-45	0.0	0.0010023541226276901	0.0	0.13852064121219698	0.0
46-47	0.0	0.001096324821624036	0.0	0.16541192290831797	0.0
48-49	0.0	0.001096324821624036	0.0	0.19041595973292902	0.0
50-51	0.0	0.0011981264122034108	0.0	0.21433933351908208	0.0
52-53	0.0	0.0012216190869524972	0.0	0.2673857931025194	0.0
54-55	0.0	0.0012216190869524972	0.0	0.3281456808952401	0.0
56-57	0.0	0.001237280870118555	0.0	0.38803633972224455	0.0
58-59	0.0	0.0012451117617015839	0.0	0.42968885205237484	0.0
60-61	0.0	0.0012607735448676416	0.0	0.4598456155386189	0.0
62-63	0.0	0.0012686044364506703	0.0	0.4833304593961224	0.0
64-65	0.0	0.0014252222681112468	0.0	0.4916233735825499	0.0
66-67	0.0	0.0014330531596942757	0.0	0.5001825380828004	0.0
68-69	0.0	0.0014800385091924485	0.0	0.5083266653291504	0.0
70-71	0.0	0.001511362075524564	0.0	0.5147949817767322	0.0
72-73	0.0	0.001519192967107593	0.0	0.5201513116195239	0.0
74-75	0.0	0.0015740092081887947	0.0	0.5241607281100347	0.0
76-77	0.0	0.0016366563408530253	0.0	0.5284285640227855	0.0
78-79	0.0	0.0016601490156021117	0.0	0.5312946703421739	0.0
80-81	0.0	0.0016601490156021117	0.0	0.5320620977173107	0.0
82-83	0.0	0.0016679799071851406	0.0	0.5325319512122926	0.0
84-85	0.0	0.0016758107987681695	0.0	0.5327198926102852	0.0
86-87	0.0	0.0017227961482663423	0.0	0.5328295250924475	0.0
88	0.0	0.0017854432809305731	0.0	0.5328451868756137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6400	0.0	42.73029	1
GTATCAA	11375	0.0	33.29679	1
ATCAACG	12905	0.0	28.329437	3
TCAACGC	12905	0.0	28.297571	4
CAACGCA	13150	0.0	27.676533	5
AACGCAG	13740	0.0	26.637745	6
TATCAAC	14015	0.0	26.555204	2
ACGCAGA	16070	0.0	22.69875	7
CGCAGAG	16535	0.0	22.03554	8
TACATGG	16570	0.0	20.62399	2
GTACATG	16750	0.0	20.598772	1
ACATGGG	17225	0.0	19.242876	3
GCAGAGT	19780	0.0	18.233393	9
CATGGGG	13105	0.0	17.227787	4
GAGTACT	10905	0.0	17.216303	12-13
GTGGTAT	3980	0.0	17.04885	1
AGAGTAC	17315	0.0	16.779753	10-11
TAGACCG	425	1.8189894E-12	16.449554	5
CAGAGTA	19165	0.0	16.211437	10-11
ACCGAAC	765	0.0	16.127016	8
>>END_MODULE