FastQCFastQC Report
Fri 27 May 2016
SRR2140283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745398
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.042.897771
GTATCAA100850.033.666571
ATCAACG116350.028.471543
TCAACGC116400.028.4235744
CAACGCA117850.028.073865
AACGCAG123750.026.8021416
TATCAAC125850.026.6833442
ACGCAGA143500.023.055787
CGCAGAG148300.022.198138
GTACATG154950.020.739151
TACATGG152700.020.5848922
ACATGGG155200.019.8807093
GCAGAGT180150.018.0213579
CATGGGG120200.017.8001824
GAGTACT98400.017.33779312-13
AGAGTAC160350.016.49890110-11
CTAACGC4101.6370905E-1116.1190363
CAGAGTA176300.016.10739310-11
GAGTACA114850.016.0760561
GTACTAG14150.016.054961
Produced by FastQC (version 0.11.5)