##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140283_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5745398
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.056243797209525	33.0	31.0	34.0	30.0	34.0
2	32.187843905678946	33.0	31.0	34.0	30.0	34.0
3	32.09471110617576	33.0	31.0	34.0	30.0	34.0
4	35.98047654836097	37.0	35.0	37.0	35.0	37.0
5	35.85194045738868	37.0	35.0	37.0	35.0	37.0
6	35.90347822726989	37.0	35.0	37.0	35.0	37.0
7	35.85796493123714	37.0	35.0	37.0	35.0	37.0
8	35.849461603878446	37.0	35.0	37.0	35.0	37.0
9	37.604314270308166	39.0	37.0	39.0	35.0	39.0
10-11	37.48687688825039	39.0	37.0	39.0	34.5	39.0
12-13	37.46262617489685	39.0	37.0	39.0	34.0	39.0
14-15	38.759538677738256	40.0	38.0	41.0	34.5	41.0
16-17	38.6101313607865	40.0	38.0	41.0	34.0	41.0
18-19	38.67385114138307	40.0	38.0	41.0	34.0	41.0
20-21	38.70018582736556	40.0	38.0	41.0	34.0	41.0
22-23	38.62845808319446	40.0	38.0	41.0	34.0	41.0
24-25	38.57620750948169	40.0	38.0	41.0	34.0	41.0
26-27	38.37305218768766	40.0	38.0	41.0	34.0	41.0
28-29	38.265216646862385	40.0	38.0	41.0	34.0	41.0
30-31	38.147699252865735	40.0	38.0	41.0	33.5	41.0
32-33	37.9725389922734	40.0	37.0	41.0	33.0	41.0
34-35	38.06285024222865	40.0	37.0	41.0	33.0	41.0
36-37	38.03910161523547	40.0	37.0	41.0	33.0	41.0
38-39	37.99419742273635	40.0	37.0	41.0	33.0	41.0
40-41	37.92593904415668	40.0	37.0	41.0	33.0	41.0
42-43	37.99497679312222	40.0	37.0	41.0	33.0	41.0
44-45	38.00221642245645	40.0	37.0	41.0	33.0	41.0
46-47	37.94126155086714	40.0	37.0	41.0	33.0	41.0
48-49	37.85636412988161	40.0	37.0	41.0	33.0	41.0
50-51	37.73720196210765	40.0	37.0	41.0	32.5	41.0
52-53	37.56521448094226	40.0	36.0	41.0	32.0	41.0
54-55	37.417597141442855	39.0	36.0	41.0	32.0	41.0
56-57	37.27921016916397	39.0	36.0	41.0	31.0	41.0
58-59	37.11050013389263	39.0	35.0	41.0	31.0	41.0
60-61	36.92627622972242	39.0	35.0	40.5	31.0	41.0
62-63	36.71421722038508	38.5	35.0	40.0	31.0	41.0
64-65	36.419155491304934	38.0	35.0	40.0	30.5	41.0
66-67	36.12794483012124	37.5	34.5	40.0	30.0	41.0
68-69	35.814128443921675	37.0	34.0	39.5	30.0	41.0
70-71	35.50518935473444	36.5	34.0	39.0	29.0	41.0
72-73	35.22307521562335	36.0	34.0	39.0	29.0	40.5
74-75	34.88967865847204	35.5	34.0	38.5	29.0	40.0
76-77	33.6125233155569	34.5	32.0	36.5	28.0	39.0
78-79	34.3113943795493	35.0	33.5	37.0	29.0	39.0
80-81	34.21746528677665	35.0	34.0	37.0	29.0	39.0
82-83	33.988940893513984	35.0	33.5	36.0	29.0	38.5
84-85	33.74702219546169	35.0	33.0	36.0	29.0	37.0
86-87	33.540476795839936	35.0	33.0	35.5	29.0	37.0
88-89	33.374088085707065	35.0	33.0	35.0	29.0	36.5
90-91	33.24705023095056	35.0	33.0	35.0	29.0	36.0
92-93	33.19189865322975	35.0	33.0	35.0	29.0	36.0
94-95	33.12169419690354	35.0	33.0	35.0	29.0	36.0
96-97	33.15932777280245	35.0	33.0	35.0	29.0	35.0
98-99	33.27731119040611	35.0	33.0	35.0	29.0	35.0
100	32.09077409087247	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	20.0
21	215.0
22	858.0
23	3015.0
24	8207.0
25	17387.0
26	31695.0
27	51100.0
28	72840.0
29	96710.0
30	123378.0
31	153187.0
32	189640.0
33	239387.0
34	316145.0
35	435070.0
36	663163.0
37	1118137.0
38	1419519.0
39	775760.0
40	29961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.597535975749636	20.380172095997526	13.826022148509121	24.196269779743716
2	14.8613203123613	21.12168730521367	40.77355476504848	23.24343761737655
3	18.30277226175253	25.751764765469865	30.413701023483036	25.531761949294573
4	12.567588877219645	17.597632052644567	38.75111872145324	31.083660348682546
5	13.030481091127195	38.00897692379188	35.907503709925756	13.053038275155176
6	30.595304276570566	37.85226019154809	18.428366494366447	13.124069037514893
7	27.069125585381553	32.3605083581677	22.93653111586003	17.633834940590713
8	24.834658974017117	36.80954739776078	20.924364160672592	17.431429467549506
9	25.5426517014139	16.609763153048753	21.222968365289923	36.62461678024743
10-11	24.457548458783883	26.73847834388497	28.759478455626574	20.044494741704575
12-13	24.985849544278743	24.053425019467756	29.06178127259417	21.89894416365933
14-15	21.98570925808795	25.62195865282092	26.51349828158119	25.878833807509942
16-17	21.828739942824953	28.79452620589718	27.21507183606327	22.161662015214596
18-19	21.793766408865032	28.176882898088735	28.732348571528533	21.2970021215177
20-21	22.542783541042958	27.34952656130335	28.603164380700413	21.504525516953283
22-23	22.307752631792876	27.244484199581503	28.45186805963607	21.995895108989547
24-25	22.083527794267603	27.35859675771051	28.578553468334274	21.97932197968761
26-27	22.080914685476703	27.622750597612338	28.378575058759587	21.91775965815137
28-29	22.00425943042865	27.74853759119491	28.371066378521036	21.876136599855403
30-31	22.25899782684406	27.493316875401447	28.37267192888609	21.875013368868405
32-33	22.016092102798407	27.607010592007477	28.341468827144627	22.035428478049493
34-35	22.153830142061047	27.646798114947686	28.378199959986766	21.8211717830045
36-37	22.148612626702427	27.63368575968517	28.278708265166706	21.938993348445695
38-39	22.007850445975176	27.68843030279273	28.356537821474515	21.947181429757574
40-41	22.244150338196214	27.51813242332601	28.250989776801287	21.986727461676487
42-43	22.175668307090902	27.647543792647276	28.073971201333634	22.102816698928184
44-45	22.203762444301216	27.796384288311977	27.922785263790388	22.077068003596416
46-47	22.165047633979555	27.703521732394353	27.776246002669993	22.355184630956103
48-49	22.16519107124278	27.802446869980514	27.80511126328174	22.22725079549496
50-51	22.12002242346002	27.910435161199448	27.680594695960075	22.288947719380452
52-53	22.21226152913249	27.858317765028602	27.582148743995017	22.347271961843894
54-55	22.231115544235532	27.770508846062143	27.651116159183285	22.34725945051904
56-57	22.276025672580314	27.805311347992202	27.584022280549554	22.33464069887793
58-59	22.09809811470893	27.98944133483906	27.650316717481527	22.262143832970484
60-61	22.285817980550483	27.914788297532674	27.632762026814017	22.166631695102826
62-63	22.28713095534653	27.948381283141078	27.49635046151755	22.26813729999484
64-65	22.122057837827626	27.97330734584007	27.543676498590276	22.36095831774203
66-67	22.216031866463556	27.96817197359368	27.40623531161008	22.409560848332692
68-69	22.188474489747883	27.947421881292833	27.51256604693347	22.35153758202582
70-71	22.15167023268636	27.719500841261517	27.599710962304265	22.52911796374786
72-73	22.244429469679638	27.767469822430403	27.571474545270156	22.4166261626198
74-75	22.203204991618	27.93968823217724	27.44731361607191	22.409793160132846
76-77	22.255340086303114	27.835406765392186	27.41117113563846	22.498082012666238
78-79	22.244372934581964	27.821504741747276	27.444973516618187	22.48914880705257
80-81	22.20199717490409	27.844278279272057	27.47702707956414	22.47669746625971
82-83	22.2425143230909	27.879510629489236	27.558921591268913	22.31905345615095
84-85	22.431405264357974	27.919065177462105	27.41125968441737	22.238269873762547
86-87	22.22204607719145	27.887410444930605	27.539042546070736	22.351500931807212
88-89	22.28925952507218	27.853367581480303	27.529838692086013	22.32753420136151
90-91	22.322708238785605	27.911543921749924	27.54116120870019	22.224586630764282
92-93	22.403884951919338	27.865250756099698	27.54963234613633	22.18123194584463
94-95	22.10241589227761	28.020054324820094	27.623200931282632	22.254328851619665
96-97	22.09689250090667	28.18908652033984	27.73971221736143	21.974308761392063
98-99	23.26221434997046	30.110380224822546	27.86451909157152	18.762886333635475
100	29.648753680618846	41.55725854674816	28.793987772632995	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	533.0
1	565.0
2	999.5
3	1482.0
4	2076.5
5	3611.5
6	4962.5
7	5754.5
8	6165.5
9	5939.5
10	5271.5
11	4602.5
12	4941.5
13	6763.5
14	10865.5
15	16525.5
16	21514.5
17	24712.5
18	25903.0
19	25607.0
20	24455.5
21	23163.5
22	23085.5
23	25421.5
24	30302.0
25	37652.0
26	47648.0
27	59114.0
28	71079.0
29	85322.0
30	99735.0
31	113559.0
32	128093.0
33	142120.5
34	155534.0
35	169431.5
36	183471.0
37	195488.0
38	205365.5
39	213252.0
40	222084.5
41	230227.5
42	235473.5
43	241063.0
44	247128.0
45	251969.0
46	254592.0
47	255949.0
48	255484.5
49	251449.5
50	245198.0
51	237671.5
52	227510.0
53	216172.0
54	204062.0
55	189472.5
56	172690.5
57	154757.0
58	137139.5
59	117551.0
60	97135.0
61	79445.0
62	64371.0
63	50423.0
64	37830.5
65	28657.0
66	21823.0
67	16769.5
68	12872.0
69	9474.5
70	6920.0
71	5001.0
72	3615.5
73	2585.5
74	1788.0
75	1200.0
76	769.5
77	508.0
78	341.0
79	222.0
80	150.5
81	97.0
82	64.5
83	48.0
84	40.0
85	29.5
86	20.5
87	15.5
88	11.5
89	8.5
90	6.5
91	6.5
92	7.0
93	4.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.9588898802136943E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001035611458074793
18-19	0.0038030437578040724
20-21	0.0
22-23	0.0015579577697703972
24-25	6.616198421183536E-4
26-27	0.013629234315712288
28-29	0.007146477070048984
30-31	8.46869716340552E-4
32-33	0.007259371112536714
34-35	0.005089297839509564
36-37	0.0022889851735625215
38-39	0.0017639138504475442
40-41	0.0038384965606801133
42-43	0.008521468883434382
44-45	0.010013498048651662
46-47	0.009304204155203772
48-49	0.007879978915427846
50-51	0.0340883499863694
52-53	0.012878822915801894
54-55	8.70208330775082E-5
56-57	0.009066479865917704
58-59	0.0067458855530391545
60-61	1.5979365844884501E-4
62-63	0.005543295459869368
64-65	0.0024809340726993315
66-67	0.024100069521417865
68-69	0.032961602034249526
70-71	0.0157562774947307
72-73	0.011779983353672295
74-75	0.01731213298495016
76-77	0.002261894683767908
78-79	0.003101991823416391
80-81	0.001972006522270715
82-83	0.025384709151438985
84-85	0.001065371545431438
86-87	0.009675297486043032
88-89	9.774191152902797E-4
90-91	0.002811358907893702
92-93	0.001283286160227655
94-95	6.803154748841385E-4
96-97	0.0028914092988241687
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	125.0
20-21	396.0
22-23	935.0
24-25	2004.0
26-27	3276.0
28-29	7846.0
30-31	16387.0
32-33	19973.0
34-35	27364.0
36-37	42076.0
38-39	50481.0
40-41	51711.0
42-43	55014.0
44-45	61981.0
46-47	61174.0
48-49	57977.0
50-51	52513.0
52-53	49875.0
54-55	52835.0
56-57	55068.0
58-59	55961.0
60-61	54866.0
62-63	54568.0
64-65	56012.0
66-67	58762.0
68-69	59376.0
70-71	58476.0
72-73	54930.0
74-75	56170.0
76-77	55592.0
78-79	59232.0
80-81	60843.0
82-83	59378.0
84-85	58674.0
86-87	62352.0
88-89	67307.0
90-91	72247.0
92-93	80433.0
94-95	98081.0
96-97	217420.0
98-99	1205674.0
100-101	2470033.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.6955936023331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.90386863185323	77.04365389552837
2	7.023925265096374	12.038388900217173
3	1.7898578450478895	4.6014879148551495
4	0.6304413297761222	2.161041759464361
5	0.27422403487525	1.174989572433072
6	0.14112707008252298	0.7256380826447922
7	0.07658641903149926	0.4594183048546944
8	0.04648276428056735	0.3186694461840436
9	0.03014642396388193	0.23250741269152153
>10	0.08098984721891689	0.9975026719218233
>50	0.0014970189735271041	0.08574729670617917
>100	8.533498002320179E-4	0.16095474249888128
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.740523458949232E-5	0.0	1.740523458949232E-5	0.0
3	0.0	1.740523458949232E-5	0.0	1.740523458949232E-5	0.0
4	0.0	3.481046917898464E-5	0.0	3.481046917898464E-5	0.0
5	0.0	3.481046917898464E-5	0.0	3.481046917898464E-5	0.0
6	0.0	5.221570376847696E-5	0.0	3.481046917898464E-5	0.0
7	0.0	5.221570376847696E-5	0.0	3.481046917898464E-5	0.0
8	0.0	5.221570376847696E-5	0.0	3.481046917898464E-5	0.0
9	0.0	1.0443140753695392E-4	0.0	5.221570376847696E-5	0.0
10-11	0.0	1.0443140753695392E-4	0.0	8.70261729474616E-5	0.0
12-13	0.0	1.0443140753695392E-4	0.0	8.70261729474616E-5	0.0
14-15	0.0	1.9145758048441552E-4	0.0	8.70261729474616E-5	0.0
16-17	0.0	2.0886281507390784E-4	0.0	8.70261729474616E-5	0.0
18-19	0.0	2.4367328425289246E-4	0.0	1.0443140753695392E-4	0.0
20-21	0.0	2.784837534318771E-4	0.0	2.0016019777916168E-4	0.0
22-23	0.0	2.9588898802136943E-4	0.0	2.3497066695814633E-4	0.0
24-25	0.0	3.3940207449510023E-4	0.0	3.481046917898464E-4	0.0
26-27	0.0	3.655099263793387E-4	0.0	4.87346568505785E-4	0.0
28-29	0.0	3.655099263793387E-4	0.0	0.0012531768904434472	0.0
30-31	0.0	4.35130864737308E-4	0.0	0.004272985091720365	0.0
32-33	0.0	4.35130864737308E-4	0.0	0.009738228752820953	0.0
34-35	0.0	5.221570376847696E-4	0.0	0.01625648910658583	0.0
36-37	0.0	6.091832106322312E-4	0.0	0.02742194709574515	0.0
38-39	0.0	6.613989144007081E-4	0.0	0.05537475384646982	0.0
40-41	0.0	7.832355565271544E-4	0.0	0.08565115941489171	0.0
42-43	0.0	7.919381738219005E-4	0.0	0.10590214985976602	0.0
44-45	0.0	8.70261729474616E-4	0.0	0.13002580500080238	0.0
46-47	0.0	9.311800505378391E-4	0.0	0.15533301609392422	0.0
48-49	0.0	9.398826678325853E-4	0.0	0.17729842214586353	0.0
50-51	0.0	9.920983716010623E-4	0.0	0.19924642296321332	0.0
52-53	0.0	9.920983716010623E-4	0.0	0.2508964566075318	0.0
54-55	0.0	0.0010008009888958086	0.0	0.3112839180157754	0.0
56-57	0.0	0.001026908840780047	0.0	0.3702354475703859	0.0
58-59	0.0	0.001026908840780047	0.0	0.4102326766570392	0.0
60-61	0.0	0.0011052323964327623	0.0	0.43963882049598657	0.0
62-63	0.0	0.001148745482906493	0.0	0.4640931750942232	0.0
64-65	0.0	0.0013227978288014163	0.0	0.4721692039437477	0.0
66-67	0.0	0.0013315004460961624	0.0	0.47988842548418753	0.0
68-69	0.0	0.0013837161498646395	0.0	0.4877642941359328	0.0
70-71	0.0	0.0014272292363383702	0.0	0.49416942046486595	0.0
72-73	0.0	0.0014446344709278627	0.0	0.4992604515822925	0.0
74-75	0.0	0.001462039705517355	0.0	0.5031940345995178	0.0
76-77	0.0	0.0015055527919910858	0.0	0.5069274574189638	0.0
78-79	0.0	0.001514255409285832	0.0	0.5092945693231348	0.0
80-81	0.0	0.001514255409285832	0.0	0.5099994813240092	0.0
82-83	0.0	0.001522958026580578	0.0	0.5106086645346415	0.0
84-85	0.0	0.0015316606438753242	0.0	0.5109132561399576	0.0
86-87	0.0	0.0015490658784648166	0.0	0.511035092782084	0.0
88	0.0	0.0016883077551807552	0.0	0.5110524980166735	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5835	0.0	42.89777	1
GTATCAA	10085	0.0	33.66657	1
ATCAACG	11635	0.0	28.47154	3
TCAACGC	11640	0.0	28.423574	4
CAACGCA	11785	0.0	28.07386	5
AACGCAG	12375	0.0	26.802141	6
TATCAAC	12585	0.0	26.683344	2
ACGCAGA	14350	0.0	23.05578	7
CGCAGAG	14830	0.0	22.19813	8
GTACATG	15495	0.0	20.73915	1
TACATGG	15270	0.0	20.584892	2
ACATGGG	15520	0.0	19.880709	3
GCAGAGT	18015	0.0	18.021357	9
CATGGGG	12020	0.0	17.800182	4
GAGTACT	9840	0.0	17.337793	12-13
AGAGTAC	16035	0.0	16.498901	10-11
CTAACGC	410	1.6370905E-11	16.119036	3
CAGAGTA	17630	0.0	16.107393	10-11
GAGTACA	11485	0.0	16.076056	1
GTACTAG	1415	0.0	16.05496	1
>>END_MODULE