FastQCFastQC Report
Fri 27 May 2016
SRR2140282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8053911
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77500.041.9759141
GTATCAA137450.033.134861
TCAACGC155250.028.6418024
ATCAACG155450.028.5516363
CAACGCA156850.028.2439485
AACGCAG163400.027.1371336
TATCAAC168850.026.5802862
ACGCAGA190800.023.1097627
CGCAGAG194000.022.7072098
TACATGG203650.021.0614432
GTACATG208850.020.9537451
ACATGGG208350.019.8503993
CATGGGG157200.018.4007324
GCAGAGT239200.018.2951099
AGAGTAC208250.016.80542810-11
GAGTACT130950.016.72604612-13
CAGAGTA227900.016.45656410-11
ATGGGGG103300.015.12422855
ATTCGCG2500.004222485714.73839390-91
GTACTTT151400.014.61786714-15
Produced by FastQC (version 0.11.5)