##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8053911
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.070324715532614	33.0	31.0	34.0	30.0	34.0
2	32.2046421918494	33.0	31.0	34.0	30.0	34.0
3	32.11424424729799	33.0	31.0	34.0	30.0	34.0
4	35.99119160864827	37.0	35.0	37.0	35.0	37.0
5	35.86208116777054	37.0	35.0	37.0	35.0	37.0
6	35.9146727596071	37.0	35.0	37.0	35.0	37.0
7	35.86579638141023	37.0	35.0	37.0	35.0	37.0
8	35.8557092324462	37.0	35.0	37.0	35.0	37.0
9	37.61732008213153	39.0	37.0	39.0	35.0	39.0
10-11	37.495963824283635	39.0	37.0	39.0	34.5	39.0
12-13	37.47548700252585	39.0	37.0	39.0	34.5	39.0
14-15	38.787255222959374	40.0	38.0	41.0	35.0	41.0
16-17	38.63538931582433	40.0	38.0	41.0	34.0	41.0
18-19	38.698650941139036	40.0	38.0	41.0	34.0	41.0
20-21	38.72634962486541	40.0	38.0	41.0	34.0	41.0
22-23	38.658193573670204	40.0	38.0	41.0	34.0	41.0
24-25	38.608261253019634	40.0	38.0	41.0	34.0	41.0
26-27	38.41338864320622	40.0	38.0	41.0	34.0	41.0
28-29	38.308514326853995	40.0	38.0	41.0	34.0	41.0
30-31	38.19038248478448	40.0	38.0	41.0	33.5	41.0
32-33	38.01855134342787	40.0	37.0	41.0	33.0	41.0
34-35	38.10549227256529	40.0	38.0	41.0	33.0	41.0
36-37	38.082920543448715	40.0	37.5	41.0	33.0	41.0
38-39	38.03972880681112	40.0	37.0	41.0	33.0	41.0
40-41	37.97284012451172	40.0	37.0	41.0	33.0	41.0
42-43	38.03761888187568	40.0	37.0	41.0	33.0	41.0
44-45	38.04669516791513	40.0	37.0	41.0	33.0	41.0
46-47	37.98823270174684	40.0	37.0	41.0	33.0	41.0
48-49	37.90370082862984	40.0	37.0	41.0	33.0	41.0
50-51	37.78639567355261	40.0	37.0	41.0	33.0	41.0
52-53	37.61627520290598	40.0	36.5	41.0	32.0	41.0
54-55	37.471268593172525	39.0	36.0	41.0	32.0	41.0
56-57	37.3367390135278	39.0	36.0	41.0	31.5	41.0
58-59	37.168589968988876	39.0	35.5	41.0	31.0	41.0
60-61	36.9856403884973	39.0	35.0	40.5	31.0	41.0
62-63	36.77570440765676	38.5	35.0	40.0	31.0	41.0
64-65	36.485166893561185	38.0	35.0	40.0	31.0	41.0
66-67	36.198426534570814	37.5	35.0	40.0	30.0	41.0
68-69	35.890169891352144	37.0	34.0	40.0	30.0	41.0
70-71	35.58789566162393	36.5	34.0	39.0	30.0	41.0
72-73	35.303069687856386	36.0	34.0	39.0	29.5	40.5
74-75	34.97203295539076	35.5	34.0	38.5	29.0	40.0
76-77	33.688149365127906	34.5	32.0	36.5	28.0	39.0
78-79	34.371672737449416	35.0	33.5	37.0	29.0	39.0
80-81	34.27812988579565	35.0	34.0	37.0	29.0	39.0
82-83	34.04484213194334	35.0	34.0	36.0	29.0	38.5
84-85	33.802543739061704	35.0	33.0	36.0	29.0	37.0
86-87	33.59743289799021	35.0	33.0	35.5	29.0	37.0
88-89	33.42858173216686	35.0	33.0	35.0	29.0	36.5
90-91	33.29917725736556	35.0	33.0	35.0	29.0	36.0
92-93	33.24565340506085	35.0	33.0	35.0	29.0	36.0
94-95	33.17314523376821	35.0	33.0	35.0	29.0	36.0
96-97	33.20537427657355	35.0	33.0	35.0	29.0	35.0
98-99	33.31940393289494	35.0	33.0	35.0	29.5	35.0
100	32.143432235172874	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	33.0
21	264.0
22	1207.0
23	4220.0
24	11016.0
25	24054.0
26	44187.0
27	69575.0
28	99648.0
29	132917.0
30	169229.0
31	210938.0
32	260164.0
33	328610.0
34	435263.0
35	599939.0
36	920816.0
37	1562517.0
38	2010751.0
39	1121171.0
40	47385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.421006514723096	20.45662039225415	13.777827939742568	24.34454515328019
2	14.957019018461962	21.18174635900496	40.74246661032137	23.118768012211707
3	18.290067464335536	25.68158100097034	30.49919386730752	25.52915766738661
4	12.553789084582634	17.63697165265422	38.707964366628836	31.101274896134314
5	13.052652307680082	37.93446438630871	35.93130095428172	13.08158235172949
6	30.609923551427375	37.85727207564126	18.43062333318558	13.102181039745783
7	27.032369739372587	32.37787206737199	22.943039723185418	17.64671847007001
8	24.710069430864085	36.90897503088872	20.99381281963508	17.387142718612115
9	25.437989071396494	16.611544378873816	21.2693683851237	36.68109816460599
10-11	24.441491841665496	26.716430067330023	28.867428507715072	19.974649583289413
12-13	24.87154377544028	24.068666763265696	29.110422253238212	21.94936720805581
14-15	21.910659305770825	25.75364316789694	26.469109231527394	25.86658829480485
16-17	21.848461221508078	28.894914034480074	27.19016811795169	22.066456626060155
18-19	21.741567913507307	28.298221834706595	28.681889623772378	21.278320628013724
20-21	22.477130290727228	27.403085750017446	28.63218439900537	21.487599560249954
22-23	22.254646965944293	27.38402686755658	28.394410167351005	21.966915999148124
24-25	22.076083095488272	27.421902363049682	28.54926932506651	21.952745216395535
26-27	22.085367877397584	27.697171247262986	28.3509859471	21.866474928239434
28-29	21.967010801220162	27.819869796524976	28.35643398663426	21.8566854156206
30-31	22.231418156959105	27.61861090331536	28.317433456329738	21.832537483395797
32-33	21.982916396829015	27.70569932711079	28.30022350143254	22.01116077462766
34-35	22.140107240809648	27.724093745990245	28.33179041428755	21.804008598912556
36-37	22.112321483811744	27.678983481742048	28.286276081526214	21.92241895291999
38-39	22.01434969907787	27.740467415287767	28.337679958134338	21.907502927500026
40-41	22.21050264490182	27.610657401326783	28.235204175800483	21.94363577797091
42-43	22.136223180004848	27.662341023931464	28.109809732525097	22.091626063538595
44-45	22.17185330472756	27.84513373602823	27.922541962815234	22.060470996428972
46-47	22.17257553712664	27.73548462424787	27.771929601331642	22.320010237293854
48-49	22.177675312553262	27.772928477046943	27.81288271564574	22.236513494754057
50-51	22.08960609601504	27.929514017643864	27.72507432455739	22.255805561783703
52-53	22.234137466102922	27.844911802149923	27.677110172805484	22.243840558941667
54-55	22.232761272928787	27.76771923090282	27.69332350908928	22.306195987079114
56-57	22.277369325681143	27.854462707716536	27.623370744423458	22.24479722217886
58-59	22.159418580840985	27.969667746951654	27.715472590366918	22.155441081840443
60-61	22.283165317688766	27.985353224478605	27.617864230418537	22.113617227414093
62-63	22.192840371165605	28.055764529038346	27.551013745612007	22.200381354184042
64-65	22.1423402095026	28.047151040875455	27.551712076945318	22.258796672676628
66-67	22.168607273658182	28.061047002411023	27.391675586498383	22.378670137432415
68-69	22.19867698397859	28.006569434215073	27.505760280731916	22.28899330107442
70-71	22.1411539615852	27.81684486523307	27.559150677973683	22.48285049520805
72-73	22.231922707147174	27.796793019683815	27.577455125900457	22.393829147268562
74-75	22.148850352114856	27.963293062761952	27.503914965283492	22.3839416198397
76-77	22.23247242587312	27.927509592107334	27.401527688472477	22.43849029354707
78-79	22.253542701513037	27.835448960454368	27.412884405287674	22.498123932744925
80-81	22.167333865160163	27.907175556788076	27.482618358973447	22.442872219078307
82-83	22.27978055946312	27.867061665077554	27.555189866698242	22.297967908761084
84-85	22.363363077368998	27.870246992815883	27.487729032525205	22.27866089728991
86-87	22.18660262872264	27.92497125134036	27.552957460052347	22.335468659884654
88-89	22.31318069966873	27.874603671872883	27.531694936571704	22.280520691886682
90-91	22.341876372032658	27.887688090163554	27.524746436505325	22.245689101298463
92-93	22.342232928051892	27.85747144184129	27.600176216508327	22.20011941359849
94-95	22.132517622832538	27.95662873465715	27.666194216145616	22.244659426364695
96-97	22.0701745271042	28.109847895508132	27.79712126134224	22.022856316045427
98-99	23.236698319367942	30.02634384919588	27.987742604928563	18.749215226507616
100	22.221062678415745	31.080049831395083	21.596713562966492	25.102173927222683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	959.0
1	912.5
2	1409.5
3	2063.5
4	2744.0
5	4590.5
6	6202.5
7	7095.5
8	7565.0
9	7268.0
10	6558.0
11	5899.5
12	6463.0
13	8920.0
14	14409.5
15	21887.0
16	28525.5
17	33053.5
18	34915.5
19	34776.5
20	33590.5
21	32257.5
22	32216.0
23	34862.5
24	40960.5
25	50916.5
26	64374.5
27	79923.0
28	97055.5
29	117035.0
30	137453.0
31	157299.0
32	178945.5
33	199377.0
34	219199.0
35	239177.5
36	258916.5
37	277823.5
38	292132.0
39	302742.5
40	314136.0
41	324271.0
42	331136.0
43	338164.5
44	343663.5
45	351202.0
46	358718.5
47	359990.0
48	357812.0
49	352547.5
50	344433.5
51	333167.5
52	318581.0
53	301153.5
54	281459.0
55	258844.0
56	236461.5
57	213523.5
58	188450.5
59	164349.0
60	139154.0
61	113734.0
62	91028.0
63	71204.5
64	53796.0
65	40491.0
66	30656.5
67	23295.0
68	17686.5
69	13085.0
70	9765.5
71	7194.5
72	5248.5
73	3847.0
74	2763.5
75	1841.5
76	1153.0
77	718.0
78	485.5
79	348.0
80	240.0
81	158.5
82	113.0
83	74.0
84	48.5
85	37.0
86	28.0
87	21.0
88	14.0
89	8.5
90	7.0
91	7.0
92	6.0
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.228245258732062E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.877674461513171E-4
18-19	0.004084971885087879
20-21	0.0
22-23	0.001316289291080116
24-25	5.340617770371569E-4
26-27	0.013536080082903678
28-29	0.00734744037741725
30-31	7.284472083101258E-4
32-33	0.006904536911273517
34-35	0.0045984725302662816
36-37	0.0019522662095088966
38-39	0.0014592873424334493
40-41	0.0035505070827818322
42-43	0.008713397196725854
44-45	0.009837237719294128
46-47	0.008906074338231214
48-49	0.00798487752177662
50-51	0.033661085535660475
52-53	0.013060411303014323
54-55	4.805036007223753E-5
56-57	0.008553347917728032
58-59	0.00653754271586219
60-61	1.3479828323744326E-4
62-63	0.005280561771416104
64-65	0.0023733474389040854
66-67	0.023786275355860902
68-69	0.032105632219673094
70-71	0.015128240495886527
72-73	0.010646485288947826
74-75	0.016957848572521094
76-77	0.0021067340382982316
78-79	0.002722257222672228
80-81	0.001795084575450021
82-83	0.02516358576034975
84-85	9.440709199325171E-4
86-87	0.009698212469850661
88-89	9.904636283397943E-4
90-91	0.0033796029062152376
92-93	0.0012660089263367836
94-95	8.501685549603673E-4
96-97	0.0031469996105355216
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	147.0
20-21	579.0
22-23	1219.0
24-25	2324.0
26-27	4126.0
28-29	9813.0
30-31	20560.0
32-33	26208.0
34-35	36651.0
36-37	55846.0
38-39	64344.0
40-41	67821.0
42-43	73814.0
44-45	84188.0
46-47	84325.0
48-49	79473.0
50-51	71212.0
52-53	68897.0
54-55	74816.0
56-57	79520.0
58-59	80524.0
60-61	78823.0
62-63	79415.0
64-65	83021.0
66-67	87589.0
68-69	87254.0
70-71	84482.0
72-73	80821.0
74-75	81774.0
76-77	82462.0
78-79	88209.0
80-81	88189.0
82-83	86256.0
84-85	86860.0
86-87	92149.0
88-89	98923.0
90-91	105638.0
92-93	115238.0
94-95	138585.0
96-97	302549.0
98-99	519757.0
100-101	4599510.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.7363228664411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27353859609929	75.6471138990876
2	7.38768402555001	12.5201035764851
3	1.8688387752210038	4.750755775273541
4	0.7084265422321628	2.401178408389641
5	0.31757488960841845	1.345506419006667
6	0.16324299047214405	0.8299566447798581
7	0.0866126744241655	0.5137467681032424
8	0.05362517144882422	0.3635199873324663
9	0.039541693218377985	0.30155559149144184
>10	0.09941812661484781	1.204053437469136
>50	0.0012289280100076635	0.06614155582525977
>100	2.447222129018154E-4	0.04282490607211399
>500	2.2864887706391623E-5	0.013543030683886109
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.966531167280095E-5	0.0	0.0	0.0
3	0.0	6.208163959100119E-5	0.0	2.4832655836400475E-5	0.0
4	0.0	8.691429542740167E-5	0.0	3.7248983754600716E-5	0.0
5	0.0	8.691429542740167E-5	0.0	3.7248983754600716E-5	0.0
6	0.0	8.691429542740167E-5	0.0	4.966531167280095E-5	0.0
7	0.0	8.691429542740167E-5	0.0	4.966531167280095E-5	0.0
8	0.0	1.1174695126380214E-4	0.0	4.966531167280095E-5	0.0
9	0.0	2.855755421186055E-4	0.0	7.449796750920143E-5	0.0
10-11	1.2416327918200238E-5	2.855755421186055E-4	0.0	1.0553878730470203E-4	0.0
12-13	1.2416327918200238E-5	2.9799187003680573E-4	0.0	1.365796071002026E-4	0.0
14-15	1.2416327918200238E-5	4.221551492188081E-4	0.0	1.365796071002026E-4	0.0
16-17	1.2416327918200238E-5	4.5319596901430867E-4	0.0	1.365796071002026E-4	0.0
18-19	1.2416327918200238E-5	4.71820460891609E-4	0.0	1.614122629366031E-4	0.0
20-21	1.2416327918200238E-5	4.842367888098093E-4	0.0	2.359102304458045E-4	0.0
22-23	1.2416327918200238E-5	4.966531167280095E-4	0.0	2.545347223231049E-4	0.0
24-25	1.2416327918200238E-5	5.276939365235101E-4	0.0	3.10408197955006E-4	0.0
26-27	1.2416327918200238E-5	5.463184284008104E-4	0.0	4.1594698525970796E-4	0.0
28-29	1.2416327918200238E-5	5.463184284008104E-4	0.0	9.746817415787187E-4	0.0
30-31	1.2416327918200238E-5	6.580653796646127E-4	0.0	0.003395865685627765	0.0
32-33	1.2416327918200238E-5	6.704817075828129E-4	0.0	0.00779745393262975	0.0
34-35	1.2416327918200238E-5	7.387715111329143E-4	0.0	0.013695209693774864	0.0
36-37	1.2416327918200238E-5	8.070613146830155E-4	0.0	0.023758643471476156	0.0
38-39	1.2416327918200238E-5	8.31893970519416E-4	0.0	0.04901345445709544	0.0
40-41	1.2416327918200238E-5	8.815592821922169E-4	0.0	0.07662115958321367	0.0
42-43	1.2416327918200238E-5	9.746817415787188E-4	0.0	0.09446963096562652	0.0
44-45	1.2416327918200238E-5	0.0011174695126380213	0.0	0.11637203341333174	0.0
46-47	1.2416327918200238E-5	0.0012168001359836233	0.0	0.13972093806350727	0.0
48-49	1.2416327918200238E-5	0.0012230082999427235	0.0	0.1613936384447258	0.0
50-51	1.2416327918200238E-5	0.0013906287268384268	0.0	0.1825262285615026	0.0
52-53	1.2416327918200238E-5	0.0013906287268384268	0.0	0.23171971977341194	0.0
54-55	1.2416327918200238E-5	0.001403045054756627	0.0	0.290008171185403	0.0
56-57	1.2416327918200238E-5	0.0014154613826748272	0.0	0.3482159164659257	0.0
58-59	1.2416327918200238E-5	0.0014154613826748272	0.0	0.3873459739001337	0.0
60-61	1.2416327918200238E-5	0.0014837511862249284	0.0	0.4167602547383501	0.0
62-63	1.2416327918200238E-5	0.0015272083339386293	0.0	0.4408168900798631	0.0
64-65	1.2416327918200238E-5	0.0016389552852024314	0.0	0.4486391766683292	0.0
66-67	1.2416327918200238E-5	0.001657579777079732	0.0	0.45638696528928613	0.0
68-69	1.2416327918200238E-5	0.0016699961049979322	0.0	0.46383055387624716	0.0
70-71	1.2416327918200238E-5	0.0016824124329161323	0.0	0.47064711790333913	0.0
72-73	1.2416327918200238E-5	0.0016886205968752325	0.0	0.47630275527007937	0.0
74-75	1.2416327918200238E-5	0.0017010369247934328	0.0	0.48026356387598523	0.0
76-77	1.2416327918200238E-5	0.0017507022364662336	0.0	0.48447890720421416	0.0
78-79	1.2416327918200238E-5	0.0017755348923026342	0.0	0.4872291238380956	0.0
80-81	1.2416327918200238E-5	0.0017755348923026342	0.0	0.4881293076121651	0.0
82-83	1.2416327918200238E-5	0.0017755348923026342	0.0	0.48861354440097493	0.0
84-85	1.2416327918200238E-5	0.0018065757120981347	0.0	0.4887997893197479	0.0
86-87	1.2416327918200238E-5	0.0018376165318936353	0.0	0.4889115362710117	6.208163959100119E-6
88	1.2416327918200238E-5	0.0018996981714846364	0.0	0.48891774443497077	1.2416327918200238E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7750	0.0	41.975914	1
GTATCAA	13745	0.0	33.13486	1
TCAACGC	15525	0.0	28.641802	4
ATCAACG	15545	0.0	28.551636	3
CAACGCA	15685	0.0	28.243948	5
AACGCAG	16340	0.0	27.137133	6
TATCAAC	16885	0.0	26.580286	2
ACGCAGA	19080	0.0	23.109762	7
CGCAGAG	19400	0.0	22.707209	8
TACATGG	20365	0.0	21.061443	2
GTACATG	20885	0.0	20.953745	1
ACATGGG	20835	0.0	19.850399	3
CATGGGG	15720	0.0	18.400732	4
GCAGAGT	23920	0.0	18.295109	9
AGAGTAC	20825	0.0	16.805428	10-11
GAGTACT	13095	0.0	16.726046	12-13
CAGAGTA	22790	0.0	16.456564	10-11
ATGGGGG	10330	0.0	15.1242285	5
ATTCGCG	250	0.0042224857	14.738393	90-91
GTACTTT	15140	0.0	14.617867	14-15
>>END_MODULE