FastQCFastQC Report
Fri 27 May 2016
SRR2140281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7509857
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78450.041.3145831
GTATCAA132150.032.3582761
TCAACGC151150.027.4177424
ATCAACG151700.027.3727043
CAACGCA153500.026.8636765
AACGCAG160200.025.9975626
TATCAAC162050.025.9043392
ACGCAGA188000.022.021647
CGCAGAG192400.021.453738
TACATGG205950.021.383722
GTACATG213250.021.0191081
ACATGGG207850.020.5533923
CATGGGG163650.018.0918184
GCAGAGT230600.017.7746439
GAGTACT123700.017.16955612-13
AGAGTAC200800.017.01396410-11
GTGGTAT47000.016.5820311
CAGAGTA222250.016.10477410-11
GAGTACA146750.015.932311
TGGTATC47600.015.1601982
Produced by FastQC (version 0.11.5)