##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7509857
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06666385258734	33.0	31.0	34.0	30.0	34.0
2	32.201909437157056	33.0	31.0	34.0	30.0	34.0
3	32.108768382673595	33.0	31.0	34.0	30.0	34.0
4	35.986782171751074	37.0	35.0	37.0	35.0	37.0
5	35.859761643930106	37.0	35.0	37.0	35.0	37.0
6	35.9140803879488	37.0	35.0	37.0	35.0	37.0
7	35.86823916886833	37.0	35.0	37.0	35.0	37.0
8	35.8629317442396	37.0	35.0	37.0	35.0	37.0
9	37.62194166946188	39.0	37.0	39.0	35.0	39.0
10-11	37.503579761904916	39.0	37.0	39.0	35.0	39.0
12-13	37.47878887707183	39.0	37.0	39.0	34.5	39.0
14-15	38.78403410344565	40.0	38.0	41.0	35.0	41.0
16-17	38.63275345988612	40.0	38.0	41.0	34.0	41.0
18-19	38.70031959330251	40.0	38.0	41.0	34.0	41.0
20-21	38.72667849583931	40.0	38.0	41.0	34.0	41.0
22-23	38.65483099187094	40.0	38.0	41.0	34.0	41.0
24-25	38.59932818757815	40.0	38.0	41.0	34.0	41.0
26-27	38.400664481054285	40.0	38.0	41.0	34.0	41.0
28-29	38.293525920210676	40.0	38.0	41.0	34.0	41.0
30-31	38.17589747587927	40.0	38.0	41.0	33.5	41.0
32-33	38.00025298834777	40.0	37.0	41.0	33.0	41.0
34-35	38.09101839297776	40.0	38.0	41.0	33.0	41.0
36-37	38.06756215779112	40.0	37.5	41.0	33.0	41.0
38-39	38.020562815411765	40.0	37.0	41.0	33.0	41.0
40-41	37.95315908292794	40.0	37.0	41.0	33.0	41.0
42-43	38.01725831264031	40.0	37.0	41.0	33.0	41.0
44-45	38.022998148990084	40.0	37.0	41.0	33.0	41.0
46-47	37.95990974198488	40.0	37.0	41.0	33.0	41.0
48-49	37.86659620165294	40.0	37.0	41.0	33.0	41.0
50-51	37.74972993085764	40.0	37.0	41.0	33.0	41.0
52-53	37.57455773972616	40.0	36.0	41.0	32.0	41.0
54-55	37.42382933679986	39.0	36.0	41.0	32.0	41.0
56-57	37.28170620226449	39.0	36.0	41.0	31.0	41.0
58-59	37.10857544155425	39.0	35.0	41.0	31.0	41.0
60-61	36.92106145261326	39.0	35.0	40.0	31.0	41.0
62-63	36.71080557934378	38.0	35.0	40.0	31.0	41.0
64-65	36.408874080712806	38.0	35.0	40.0	30.5	41.0
66-67	36.11607416668798	37.0	34.5	40.0	30.0	41.0
68-69	35.798574701356245	37.0	34.0	39.5	30.0	41.0
70-71	35.49193994041531	36.0	34.0	39.0	30.0	41.0
72-73	35.20297804248204	36.0	34.0	39.0	29.0	40.5
74-75	34.875216296077504	35.5	34.0	38.5	29.0	40.0
76-77	33.59959018356451	34.5	32.0	36.5	28.0	39.0
78-79	34.298449457036725	35.0	33.5	37.0	29.0	39.0
80-81	34.210544510079814	35.0	34.0	36.5	29.0	39.0
82-83	33.98030519716836	35.0	33.5	36.0	29.0	38.5
84-85	33.74515815010324	35.0	33.0	36.0	29.0	37.0
86-87	33.54693369381616	35.0	33.0	35.5	29.0	37.0
88-89	33.37967586349451	35.0	33.0	35.0	29.0	36.5
90-91	33.25208622689585	35.0	33.0	35.0	29.0	36.0
92-93	33.19866085255413	35.0	33.0	35.0	29.0	36.0
94-95	33.13069972529364	35.0	33.0	35.0	29.0	36.0
96-97	33.166257605494124	35.0	33.0	35.0	29.0	35.0
98-99	33.28079257445962	35.0	33.0	35.0	29.0	35.0
100	32.05700907200907	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	34.0
21	244.0
22	1197.0
23	4021.0
24	10700.0
25	22896.0
26	41195.0
27	65304.0
28	94038.0
29	124192.0
30	159041.0
31	196438.0
32	243487.0
33	308073.0
34	408721.0
35	565150.0
36	871622.0
37	1475227.0
38	1854073.0
39	1022235.0
40	41965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.802487051351314	20.2258445134175	13.642683209547132	24.328985225684058
2	15.121486334560034	21.080481292786267	40.367785964499724	23.430246408153977
3	18.453128147958665	25.570708885837185	30.392311300912787	25.583851665291355
4	12.782254042919858	17.368945906693032	38.41177002438262	31.437030026004493
5	13.11967724551879	37.94672788043767	35.823558291456145	13.110036582587393
6	30.95146818374837	37.54793200456413	18.23874941959614	13.261850392091354
7	27.1367883569554	32.36525009730545	22.855614960444655	17.6423465852945
8	25.013072818829972	36.62847375123122	20.830955902356063	17.52749752758275
9	25.595760345370095	16.502897458633367	21.104636213445875	36.79670598255066
10-11	24.60586133664063	26.640866796797862	28.680066744280218	20.07320512228129
12-13	24.98544246581526	23.935049628774554	29.038216040598375	22.041291864811807
14-15	22.00336171514318	25.58670557908093	26.430902745551617	25.979029960224274
16-17	21.916392441197573	28.775341512659313	27.126080464993187	22.182185581149934
18-19	21.851243417809837	28.144438754662637	28.628554775520577	21.375763052006953
20-21	22.661549378879794	27.303575154765706	28.513689918886243	21.521185547468253
22-23	22.391664230080014	27.225554721052838	28.316714935609188	22.06606611325796
24-25	22.12790129320958	27.243642661417095	28.504231121626354	22.12422492374697
26-27	22.163741614555203	27.501406986248117	28.2920113166606	22.042840082536085
28-29	22.08318442220705	27.655023150123753	28.28971126354869	21.972081164120507
30-31	22.289595364914174	27.441053044913748	28.263599136664514	22.00575245350757
32-33	22.086991532401644	27.54887675856914	28.242563653316743	22.12156805571247
34-35	22.224900310479747	27.573213582011974	28.247695262712057	21.95419084479622
36-37	22.213221907819346	27.544347326187	28.160503985172998	22.081926780820655
38-39	22.105383980520095	27.624950991501745	28.219935775465068	22.049729252513092
40-41	22.3152616355906	27.525001939465337	28.119006966284882	22.04072945865918
42-43	22.192258918416798	27.59148014348392	27.98839153684313	22.22786940125615
44-45	22.30711139169276	27.73001124037464	27.825148483529283	22.137728884403316
46-47	22.281314395455656	27.661869989163616	27.654712283599764	22.40210333178096
48-49	22.265800875967937	27.770988975360396	27.644465683618165	22.31874446505351
50-51	22.18989769890398	27.841819166165315	27.63435474692852	22.333928388002185
52-53	22.291654807069968	27.76072228725564	27.529546929182295	22.418075976492094
54-55	22.331147450509224	27.686663665191162	27.571739912850962	22.41044897144865
56-57	22.358624634696326	27.765435979703717	27.50865597707103	22.36728340852893
58-59	22.259766744431598	27.848025841559416	27.546260906254204	22.345946507754782
60-61	22.362996298095073	27.763630956135888	27.566090761393614	22.30728198437543
62-63	22.2877131886011	27.838988650178187	27.484343843079028	22.38895431814169
64-65	22.23002355852688	27.857614660914226	27.507857784786754	22.40450399577214
66-67	22.365148772999227	27.903913949242366	27.27930815084646	22.451629126911953
68-69	22.315918480491977	27.87406165953115	27.38133757901093	22.428682280965944
70-71	22.233853611769547	27.683306539403546	27.517318570266063	22.565521278560848
72-73	22.336623623362044	27.67344282340412	27.426950466507904	22.562983086725932
74-75	22.221803266175787	27.85481991035617	27.398992957293366	22.524383866174674
76-77	22.360309312909248	27.763856907149204	27.27563366010578	22.600200119835772
78-79	22.342888819202667	27.765259715500108	27.243009159026737	22.648842306270495
80-81	22.284293223328262	27.749883849996998	27.339811016398404	22.626011910276333
82-83	22.363879082601716	27.794733142536682	27.437946989784134	22.403440785077464
84-85	22.499967848117365	27.759792570342963	27.344854662773194	22.39538491876648
86-87	22.301605183809343	27.82326158733987	27.382249723568552	22.492883505282233
88-89	22.405957303826604	27.774667450745554	27.371255730719955	22.448119514707884
90-91	22.414056904364664	27.835154737676028	27.37117858049131	22.379609777467994
92-93	22.438094279897882	27.826754839404437	27.440579900311224	22.294570980386457
94-95	22.247602497572903	27.90256920054381	27.494368866815893	22.355459435067395
96-97	22.23642512929647	27.99851196770317	27.658045022040522	22.107017880959834
98-99	23.344783669667134	29.980270916271156	27.786008068292027	18.888937345769683
100	29.87714987714988	41.50362250362251	28.619227619227622	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1088.0
1	987.5
2	1448.5
3	2127.5
4	2847.0
5	4698.5
6	6371.0
7	7415.5
8	7937.5
9	7689.5
10	6975.5
11	6205.0
12	6591.5
13	8835.0
14	14090.5
15	21348.5
16	27695.5
17	31985.0
18	33812.5
19	33589.5
20	32290.0
21	30920.0
22	31045.5
23	33864.5
24	40037.0
25	49913.5
26	63533.0
27	78841.5
28	94091.0
29	111687.0
30	128425.5
31	144754.0
32	163242.5
33	180905.5
34	198865.0
35	218019.0
36	237517.0
37	253594.0
38	265001.0
39	273738.0
40	284928.0
41	295389.0
42	300891.5
43	307449.5
44	316263.5
45	323570.5
46	327139.0
47	328511.0
48	328066.0
49	325606.5
50	322007.0
51	314855.0
52	302284.0
53	288231.5
54	271554.0
55	250830.0
56	229701.0
57	207531.0
58	184871.0
59	160007.0
60	134790.5
61	111336.5
62	88976.0
63	69185.0
64	52511.0
65	40417.5
66	30922.5
67	23785.0
68	18205.5
69	13330.5
70	9862.0
71	7272.5
72	5223.0
73	3627.5
74	2515.0
75	1722.5
76	1156.5
77	752.5
78	492.5
79	333.5
80	244.0
81	171.0
82	115.0
83	74.0
84	48.0
85	33.0
86	23.0
87	17.0
88	11.0
89	9.0
90	8.0
91	6.5
92	6.5
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.994749833452221E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.254503780830981E-4
18-19	0.00390819692039409
20-21	0.0
22-23	0.0013717056673884104
24-25	5.527843815901223E-4
26-27	0.01407787550267776
28-29	0.007880555841872047
30-31	7.21221845969986E-4
32-33	0.007104994134529348
34-35	0.005020603292359744
36-37	0.001993273142340378
38-39	0.0013887959431908934
40-41	0.003631645679896589
42-43	0.008479286986178763
44-45	0.009818615941863156
46-47	0.00896933683187024
48-49	0.007954510225197125
50-51	0.03491508434398934
52-53	0.01266243611242022
54-55	8.116077617739384E-5
56-57	0.008471605319243423
58-59	0.006704767444123042
60-61	1.6018602326602825E-4
62-63	0.0055230604357802675
64-65	0.0025206083140899586
66-67	0.02499821243819085
68-69	0.03370647654218136
70-71	0.016384378160532353
72-73	0.011872888608066087
74-75	0.017484889011317326
76-77	0.0024455681877116942
78-79	0.0028297353376750536
80-81	0.0017868911927255832
82-83	0.025229297429208538
84-85	7.68066852538845E-4
86-87	0.009592010453659483
88-89	0.0010782350784706313
90-91	0.003461478713688244
92-93	0.0013293445346390367
94-95	8.211645384004399E-4
96-97	0.0029405296014655925
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	177.0
20-21	552.0
22-23	1123.0
24-25	2547.0
26-27	4063.0
28-29	9514.0
30-31	19608.0
32-33	25294.0
34-35	34948.0
36-37	52562.0
38-39	60811.0
40-41	64393.0
42-43	69942.0
44-45	80600.0
46-47	80899.0
48-49	75454.0
50-51	68208.0
52-53	65237.0
54-55	70520.0
56-57	75050.0
58-59	74989.0
60-61	73056.0
62-63	74220.0
64-65	77431.0
66-67	81450.0
68-69	82500.0
70-71	80177.0
72-73	77034.0
74-75	78990.0
76-77	79282.0
78-79	83400.0
80-81	85397.0
82-83	81452.0
84-85	82440.0
86-87	87937.0
88-89	93684.0
90-91	100311.0
92-93	109723.0
94-95	131250.0
96-97	284012.0
98-99	1555020.0
100-101	3174600.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.84267382584332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84569287924064	76.22748815610308
2	6.949915300684832	11.79298793946482
3	1.7771706498609534	4.523397293370445
4	0.6536691019809786	2.2183613763761634
5	0.2963586754572592	1.25719312186396
6	0.15652851995081266	0.7968178897577285
7	0.09572140752477892	0.568488210974281
8	0.05802899834578276	0.3938668303273319
9	0.03967700093394682	0.3029672563763877
>10	0.12425422237494063	1.6494296879832548
>50	0.0024675174963901293	0.14007834756533363
>100	4.377064123055101E-4	0.0629505491613116
>500	2.6006654130963952E-5	0.012091477924067229
>1k	5.201308225409967E-5	0.05388186275185201
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3315832778174071E-5
2	0.0	2.6631665556348142E-5	0.0	0.0	1.3315832778174071E-5
3	1.3315832778174071E-5	2.6631665556348142E-5	0.0	1.3315832778174071E-5	1.3315832778174071E-5
4	1.3315832778174071E-5	6.657916389087036E-5	0.0	3.9947498334522216E-5	1.3315832778174071E-5
5	1.3315832778174071E-5	6.657916389087036E-5	0.0	5.3263331112696284E-5	1.3315832778174071E-5
6	1.3315832778174071E-5	6.657916389087036E-5	0.0	7.989499666904443E-5	2.6631665556348142E-5
7	1.3315832778174071E-5	6.657916389087036E-5	0.0	7.989499666904443E-5	2.6631665556348142E-5
8	1.3315832778174071E-5	7.989499666904443E-5	0.0	7.989499666904443E-5	3.9947498334522216E-5
9	1.3315832778174071E-5	1.5978999333808886E-4	0.0	1.3315832778174072E-4	3.9947498334522216E-5
10-11	2.6631665556348142E-5	1.5978999333808886E-4	0.0	1.9973749167261106E-4	3.9947498334522216E-5
12-13	2.6631665556348142E-5	1.7310582611626294E-4	0.0	2.3968499000713328E-4	3.9947498334522216E-5
14-15	2.6631665556348142E-5	2.1305332445078514E-4	0.0	2.3968499000713328E-4	3.9947498334522216E-5
16-17	2.6631665556348142E-5	2.263691572289592E-4	0.0	2.8629040473074254E-4	3.9947498334522216E-5
18-19	3.9947498334522216E-5	2.5300082278530736E-4	0.0	3.462116522325259E-4	5.3263331112696284E-5
20-21	6.657916389087036E-5	2.929483211198296E-4	0.0	4.2610664890157027E-4	6.657916389087036E-5
22-23	1.0652666222539257E-4	2.929483211198296E-4	0.0	4.660541472360925E-4	8.655291305813146E-5
24-25	1.5313207694900183E-4	3.5952748501069995E-4	0.0	4.993437291815276E-4	1.0652666222539257E-4
26-27	1.86421658894437E-4	3.9281706695613514E-4	0.0	6.325020569632684E-4	1.1984249500356664E-4
28-29	1.997374916726111E-4	3.994749833452222E-4	0.0	0.001271662030315624	1.3315832778174072E-4
30-31	1.997374916726111E-4	4.527383144579184E-4	0.0	0.0039548023351176995	1.3315832778174072E-4
32-33	2.463429063962203E-4	4.527383144579184E-4	0.0	0.008295763820802448	1.464741605599148E-4
34-35	3.0626415389800366E-4	5.725808094614851E-4	0.0	0.013821834423744687	1.5978999333808886E-4
36-37	3.0626415389800366E-4	7.190549700213999E-4	0.0	0.023662234846815327	1.5978999333808886E-4
38-39	3.0626415389800366E-4	7.32370802799574E-4	0.0	0.05005421541315633	1.5978999333808886E-4
40-41	3.462116522325259E-4	8.122657994686184E-4	0.0	0.0773450146920241	1.664479097271759E-4
42-43	3.5952748501069995E-4	8.189237158577054E-4	0.0	0.09601381224702414	1.7310582611626294E-4
44-45	3.861591505670481E-4	9.587399600285331E-4	0.0	0.11852422755852741	1.7310582611626294E-4
46-47	3.861591505670481E-4	0.0010918982878102739	0.0	0.14236622614784808	1.7310582611626294E-4
48-49	3.861591505670481E-4	0.001098556204199361	0.0	0.1651629318640821	1.7310582611626294E-4
50-51	3.9281706695613514E-4	0.0012450303647592756	0.0	0.1875601625969709	1.7310582611626294E-4
52-53	3.994749833452222E-4	0.0012516882811483628	0.0	0.2388061450437738	1.86421658894437E-4
54-55	3.994749833452222E-4	0.001271662030315624	0.0	0.2992733416894623	1.997374916726111E-4
56-57	3.994749833452222E-4	0.0012916357794828849	0.0	0.36029980331183403	1.997374916726111E-4
58-59	4.127908161233962E-4	0.001311609528650146	0.0	0.40211151823530067	1.997374916726111E-4
60-61	4.327645652906573E-4	0.0013448991105955812	0.0	0.43351690984262414	1.997374916726111E-4
62-63	4.5939623084700546E-4	0.0013448991105955812	0.0	0.4575320142580611	2.1305332445078514E-4
64-65	4.7936998001426657E-4	0.0014114782744864517	0.0	0.46600088390497985	2.1305332445078514E-4
66-67	4.926858127924406E-4	0.0014181361908755386	0.0	0.4736175402540954	2.1305332445078514E-4
68-69	4.926858127924406E-4	0.0014580836892100608	0.0	0.4813806707637709	2.1305332445078514E-4
70-71	4.926858127924406E-4	0.001498031187544583	0.0	0.48804524506924696	2.463429063962203E-4
72-73	4.926858127924406E-4	0.00150468910393367	0.0	0.4935380260902438	2.796324883416555E-4
74-75	4.926858127924406E-4	0.0015512945186572794	0.0	0.49812533048232477	2.929483211198296E-4
76-77	4.926858127924406E-4	0.0016045578497699757	0.0	0.5019802640716061	3.0626415389800366E-4
78-79	4.926858127924406E-4	0.0016245315989372369	0.0	0.504563535630572	3.0626415389800366E-4
80-81	5.060016455706147E-4	0.0016245315989372369	0.0	0.5055489072561568	3.129220702870907E-4
82-83	5.060016455706147E-4	0.0016245315989372369	0.0	0.5061414618147856	3.328958194543518E-4
84-85	5.060016455706147E-4	0.0016311895153263238	0.0	0.5063478572228473	3.328958194543518E-4
86-87	5.060016455706147E-4	0.001651163264493585	0.0	0.5064876734670181	3.328958194543518E-4
88	5.060016455706147E-4	0.0017843215922753255	0.0	0.5064943313834072	3.328958194543518E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7845	0.0	41.314583	1
GTATCAA	13215	0.0	32.358276	1
TCAACGC	15115	0.0	27.417742	4
ATCAACG	15170	0.0	27.372704	3
CAACGCA	15350	0.0	26.863676	5
AACGCAG	16020	0.0	25.997562	6
TATCAAC	16205	0.0	25.904339	2
ACGCAGA	18800	0.0	22.02164	7
CGCAGAG	19240	0.0	21.45373	8
TACATGG	20595	0.0	21.38372	2
GTACATG	21325	0.0	21.019108	1
ACATGGG	20785	0.0	20.553392	3
CATGGGG	16365	0.0	18.091818	4
GCAGAGT	23060	0.0	17.774643	9
GAGTACT	12370	0.0	17.169556	12-13
AGAGTAC	20080	0.0	17.013964	10-11
GTGGTAT	4700	0.0	16.582031	1
CAGAGTA	22225	0.0	16.104774	10-11
GAGTACA	14675	0.0	15.93231	1
TGGTATC	4760	0.0	15.160198	2
>>END_MODULE