FastQCFastQC Report
Fri 27 May 2016
SRR2140280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7090402
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89900.035.754151
GTATCAA142250.028.8405481
TCAACGC151300.026.1870274
ATCAACG151650.026.0176143
CAACGCA153800.025.7076345
AACGCAG158250.025.0369516
TATCAAC170650.023.5324152
GTACATG189400.021.8790021
TACATGG188300.021.3266432
ACGCAGA189650.020.8698447
ACATGGG190500.020.5598473
CGCAGAG195050.020.3767838
GGACCGA11500.019.4000326
CATGGGG156000.018.0355364
ACCGAAC10450.017.7910988
GCAGAGT231050.017.0051759
GAGTACT124050.016.30361412-13
AGAGTAC195150.016.2487310-11
CAGAGTA214450.016.08681310-11
AGGACCG15800.015.9506835
Produced by FastQC (version 0.11.5)