##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7090402
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05992537517619	33.0	31.0	34.0	30.0	34.0
2	32.191775163100765	33.0	31.0	34.0	30.0	34.0
3	32.09680565361456	33.0	31.0	34.0	30.0	34.0
4	35.97868611680974	37.0	35.0	37.0	35.0	37.0
5	35.84762232099111	37.0	35.0	37.0	35.0	37.0
6	35.90527532853567	37.0	35.0	37.0	35.0	37.0
7	35.85759749588246	37.0	35.0	37.0	35.0	37.0
8	35.85138275657713	37.0	35.0	37.0	35.0	37.0
9	37.60864348735093	39.0	37.0	39.0	35.0	39.0
10-11	37.48750204854393	39.0	37.0	39.0	34.5	39.0
12-13	37.459971663101754	39.0	37.0	39.0	34.0	39.0
14-15	38.757520024957685	40.0	38.0	41.0	34.5	41.0
16-17	38.606428450742285	40.0	38.0	41.0	34.0	41.0
18-19	38.67236653154504	40.0	38.0	41.0	34.0	41.0
20-21	38.69790869010256	40.0	38.0	41.0	34.0	41.0
22-23	38.62305406251558	40.0	38.0	41.0	34.0	41.0
24-25	38.56753666210221	40.0	38.0	41.0	34.0	41.0
26-27	38.35971287872559	40.0	38.0	41.0	34.0	41.0
28-29	38.2511402720216	40.0	38.0	41.0	34.0	41.0
30-31	38.13405464142366	40.0	38.0	41.0	33.0	41.0
32-33	37.9539162517076	40.0	37.0	41.0	33.0	41.0
34-35	38.04817361102116	40.0	37.0	41.0	33.0	41.0
36-37	38.02427132613191	40.0	37.0	41.0	33.0	41.0
38-39	37.97863341421343	40.0	37.0	41.0	33.0	41.0
40-41	37.90757381728716	40.0	37.0	41.0	33.0	41.0
42-43	37.97499327803841	40.0	37.0	41.0	33.0	41.0
44-45	37.97955978224656	40.0	37.0	41.0	33.0	41.0
46-47	37.915184137757905	40.0	37.0	41.0	33.0	41.0
48-49	37.823525157762234	40.0	37.0	41.0	33.0	41.0
50-51	37.703465362530444	40.0	37.0	41.0	32.5	41.0
52-53	37.52808984187209	40.0	36.0	41.0	32.0	41.0
54-55	37.374312899922174	39.0	36.0	41.0	31.5	41.0
56-57	37.23396315281075	39.0	36.0	41.0	31.0	41.0
58-59	37.057920110089356	39.0	35.0	41.0	31.0	41.0
60-61	36.86882899576531	39.0	35.0	40.0	31.0	41.0
62-63	36.65115573032864	38.0	35.0	40.0	31.0	41.0
64-65	36.34832570689754	38.0	35.0	40.0	30.5	41.0
66-67	36.04961648102588	37.0	34.5	40.0	30.0	41.0
68-69	35.731070075156595	37.0	34.0	39.5	30.0	41.0
70-71	35.41613152477086	36.0	34.0	39.0	29.0	41.0
72-73	35.13114961508805	36.0	34.0	39.0	29.0	40.0
74-75	34.80221784783076	35.0	34.0	38.5	29.0	40.0
76-77	33.532282837314	34.5	32.0	36.5	28.0	39.0
78-79	34.23582565026956	35.0	33.0	37.0	29.0	39.0
80-81	34.147757200021786	35.0	33.5	36.5	29.0	39.0
82-83	33.92386202924668	35.0	33.0	36.0	29.0	38.0
84-85	33.69454885735934	35.0	33.0	36.0	29.0	37.0
86-87	33.4978622824664	35.0	33.0	35.0	29.0	37.0
88-89	33.33417242954037	35.0	33.0	35.0	29.0	36.5
90-91	33.21241904799329	35.0	33.0	35.0	29.0	36.0
92-93	33.16192849641365	35.0	33.0	35.0	29.0	36.0
94-95	33.0942055715238	35.0	33.0	35.0	29.0	36.0
96-97	33.13509422158143	35.0	33.0	35.0	29.0	35.0
98-99	33.25608398287136	35.0	33.0	35.0	29.0	35.0
100	32.0141986718742	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	47.0
21	236.0
22	1198.0
23	3977.0
24	10584.0
25	22489.0
26	40593.0
27	63873.0
28	91633.0
29	121597.0
30	153324.0
31	190099.0
32	234776.0
33	295202.0
34	392917.0
35	539947.0
36	830370.0
37	1400541.0
38	1732333.0
39	928150.0
40	36506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02289235504559	20.09056750237857	13.535156398748619	24.351383743827217
2	15.185542371222393	21.013533506280744	40.342395254881176	23.458528867615687
3	18.59431288831647	25.444521121073432	30.23637792203891	25.724788068571193
4	12.820203988433942	17.384895807036045	38.281355556426846	31.51354464810317
5	13.237980018622359	37.838658513297275	35.722121256312406	13.201240211767965
6	31.08428830974605	37.37568053264117	18.21836053865493	13.321670618957853
7	27.351650865493944	32.19443693037432	22.71450899398934	17.739403210142388
8	25.039623987469255	36.63596224868491	20.70882581833865	17.61558794550718
9	25.65006892415973	16.449814834194168	21.026283135991445	36.873833105654654
10-11	24.74330087349067	26.476827406965075	28.5975181661068	20.182353553437448
12-13	25.16851653827244	23.89846161049825	28.918628309086003	22.01439354214331
14-15	22.152143136595075	25.47783609448378	26.319854642938438	26.050166125982706
16-17	22.00443448472633	28.603131669726935	27.041281100945945	22.351152744600785
18-19	21.979350783650776	28.041050765089814	28.554178276673493	21.42542017458592
20-21	22.718405890997673	27.20735226613749	28.39382541999797	21.68041642286687
22-23	22.49879830878051	27.06434329117745	28.262832351819924	22.174026048222114
24-25	22.28658437000186	27.169427004668123	28.35742081305271	22.186567812277307
26-27	22.306826127879656	27.33113058391542	28.24300474823136	22.119038539973563
28-29	22.164013300021352	27.513435540521574	28.265286134753875	22.0572650247032
30-31	22.406534025747778	27.325589539695137	28.199946485557113	22.06792994899997
32-33	22.178435494691485	27.422638297824037	28.169621927865933	22.229304279618546
34-35	22.297620838717776	27.46373106449661	28.19967988479897	22.03896821198664
36-37	22.32871162684498	27.402968857700543	28.099803665155683	22.168515850298796
38-39	22.217118948230375	27.48235638568492	28.15864235123674	22.141882314847965
40-41	22.40480054043893	27.391593164087425	28.062815118182836	22.140791177290804
42-43	22.279755491026076	27.49452992097642	27.90992723045031	22.315787357547197
44-45	22.33913381891862	27.603208609406966	27.77123882342689	22.286418748247524
46-47	22.344431212610296	27.586350832252894	27.607019172020912	22.4621987831159
48-49	22.32643475841653	27.673375828530578	27.57694943156385	22.42323998148904
50-51	22.29304987296743	27.753057110090424	27.5276953975344	22.42619761940774
52-53	22.392974498468334	27.676199884516116	27.390241998257693	22.540583618757857
54-55	22.385967891310028	27.619361915246866	27.476385757508314	22.51828443593479
56-57	22.445570583298885	27.65818072394235	27.421020234067743	22.475228458691017
58-59	22.306176563369583	27.75480612892998	27.478078079370853	22.46093922832958
60-61	22.4186859380301	27.706271250883326	27.466327654716586	22.408715156369986
62-63	22.381126623056467	27.782578684571458	27.354938500492633	22.48135619187944
64-65	22.340433072261405	27.783185297334363	27.374533412564254	22.501848217839978
66-67	22.441789669428417	27.782043748079378	27.22000088610549	22.556165696386717
68-69	22.331812888534994	27.80786472105842	27.331704303442688	22.5286180869639
70-71	22.31046367241962	27.60855371515704	27.367279019311475	22.713703593111862
72-73	22.39349094682148	27.630871461817318	27.36353497769336	22.61210261366784
74-75	22.28454986318537	27.81289655620282	27.294212012658203	22.60834156795361
76-77	22.456967622530914	27.66470474054724	27.20824229384667	22.67008534307518
78-79	22.371320769535117	27.670203404054572	27.23739011134747	22.72108571506284
80-81	22.367340450896762	27.73206913908664	27.23613568742161	22.66445472259499
82-83	22.38959767658949	27.782062536674086	27.325213509226803	22.50312627750962
84-85	22.520802849201846	27.72684776728071	27.270860781781952	22.481488601735485
86-87	22.383632752167003	27.727848398992887	27.320367544772562	22.568151304067545
88-89	22.4150409953033	27.717887400187376	27.33725965863616	22.52981194587317
90-91	22.44554842978416	27.76805862420659	27.324100888398934	22.462292057610313
92-93	22.50098277130748	27.72979878254019	27.36081579924172	22.40840264691061
94-95	22.21797215847412	27.84128951090547	27.48242061387341	22.458317716747
96-97	22.250785919248948	27.94927347131494	27.60975212114828	22.19018848828783
98-99	23.35175710189197	29.92141527691114	27.733422536544044	18.993405084652846
100	29.994486887188966	41.439091720472895	28.566421392338132	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1129.0
1	1028.0
2	1465.5
3	2114.0
4	2838.0
5	4655.0
6	6345.5
7	7496.5
8	8187.0
9	7939.5
10	7077.0
11	6228.0
12	6535.0
13	8687.0
14	13685.5
15	20441.0
16	26251.5
17	29972.5
18	31408.0
19	31232.5
20	29923.5
21	28532.5
22	28630.5
23	31075.5
24	36395.5
25	45122.5
26	57007.0
27	70298.0
28	84060.5
29	100158.5
30	115995.0
31	131372.0
32	149028.0
33	166496.5
34	183285.5
35	199514.5
36	217012.5
37	232626.5
38	243967.5
39	253137.5
40	262726.5
41	273557.0
42	282258.0
43	289635.5
44	297973.5
45	305231.0
46	310341.0
47	315173.5
48	315964.0
49	311877.5
50	306877.0
51	299527.0
52	288241.5
53	275453.5
54	260009.0
55	241933.5
56	222532.5
57	201195.0
58	179515.5
59	155167.0
60	130211.0
61	106874.0
62	85685.5
63	67458.5
64	51277.5
65	39069.0
66	30070.0
67	23433.5
68	17993.0
69	13207.5
70	9561.5
71	6819.0
72	4813.5
73	3316.0
74	2186.0
75	1429.0
76	982.5
77	636.5
78	403.0
79	278.0
80	198.0
81	145.0
82	105.0
83	68.5
84	43.5
85	28.0
86	16.0
87	11.0
88	6.0
89	2.5
90	2.0
91	2.0
92	1.5
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.6796788108770137E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.885250793960625E-4
18-19	0.0035823074629619026
20-21	0.0
22-23	0.001565788839832991
24-25	4.6561769584285934E-4
26-27	0.013494771834604348
28-29	0.007621268580285509
30-31	7.923810300019513E-4
32-33	0.006711107535637329
34-35	0.004385922754359489
36-37	0.0021257823254727344
38-39	0.0015071618311049933
40-41	0.003599403153513454
42-43	0.008546841127869033
44-45	0.009832164871549472
46-47	0.009306631244519147
48-49	0.00790168815059663
50-51	0.034274597325157447
52-53	0.01301536477723863
54-55	9.364219442366975E-5
56-57	0.00886266884606317
58-59	0.006281631862732632
60-61	1.1284622936012238E-4
62-63	0.0053783199921117976
64-65	0.002357091829990054
66-67	0.023265284808294553
68-69	0.03237913577358089
70-71	0.015239952669721389
72-73	0.01127425123070021
74-75	0.016951960320479794
76-77	0.0023996164879604128
78-79	0.0026838459223869587
80-81	0.0016991242019597295
82-83	0.02482792597663691
84-85	7.802623335973063E-4
86-87	0.009397750188145822
88-89	9.112569510001628E-4
90-91	0.003344667051714472
92-93	0.00105592118362932
94-95	7.810373182713712E-4
96-97	0.00287945957625153
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	220.0
20-21	791.0
22-23	1333.0
24-25	2944.0
26-27	4354.0
28-29	9152.0
30-31	18271.0
32-33	23633.0
34-35	32638.0
36-37	49369.0
38-39	57114.0
40-41	59878.0
42-43	64208.0
44-45	74731.0
46-47	74084.0
48-49	70324.0
50-51	63223.0
52-53	60764.0
54-55	65146.0
56-57	68630.0
58-59	69254.0
60-61	67598.0
62-63	68376.0
64-65	71689.0
66-67	74941.0
68-69	75780.0
70-71	74435.0
72-73	71169.0
74-75	72654.0
76-77	72763.0
78-79	77509.0
80-81	79120.0
82-83	76464.0
84-85	77186.0
86-87	81463.0
88-89	88142.0
90-91	94081.0
92-93	102899.0
94-95	124820.0
96-97	270926.0
98-99	1485509.0
100-101	3012817.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.93550309523549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.63889605137764	76.13524733025278
2	7.170638920625805	12.180836484752582
3	1.7716659595066957	4.514320187622129
4	0.6626569520054734	2.251324063925608
5	0.30940362973845525	1.3139676475663828
6	0.1468689863675962	0.748463474773144
7	0.08785561851875583	0.5223442811043529
8	0.05483188507458339	0.3725738995575899
9	0.038493928993658034	0.29425511026697554
>10	0.11664372536081509	1.5047938470356526
>50	0.0016510708269572856	0.09342297352807323
>100	3.6650728397081937E-4	0.048605306156830716
>500	2.6764319549378227E-5	0.01984539345795219
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.051786344413194E-5	0.0	0.0	0.0
3	0.0	7.051786344413194E-5	0.0	0.0	0.0
4	0.0	1.128285815106111E-4	0.0	0.0	0.0
5	0.0	1.128285815106111E-4	0.0	0.0	0.0
6	0.0	1.128285815106111E-4	0.0	0.0	1.4103572688826388E-5
7	0.0	1.128285815106111E-4	0.0	7.051786344413194E-5	1.4103572688826388E-5
8	0.0	1.4103572688826388E-4	0.0	7.051786344413194E-5	1.4103572688826388E-5
9	0.0	1.8334644495474306E-4	0.0	8.462143613295832E-5	1.4103572688826388E-5
10-11	0.0	1.8334644495474306E-4	0.0	9.872500882178472E-5	1.4103572688826388E-5
12-13	0.0	1.8334644495474306E-4	0.0	1.198803678550243E-4	2.1155359033239584E-5
14-15	0.0	2.609160947432882E-4	0.0	1.5513929957709026E-4	4.231071806647916E-5
16-17	0.0	2.8207145377652775E-4	0.0	1.8334644495474306E-4	4.231071806647916E-5
18-19	0.0	3.102785991541805E-4	0.0	2.6796788108770137E-4	4.231071806647916E-5
20-21	0.0	3.5258931722065973E-4	0.0	4.513143260424444E-4	4.231071806647916E-5
22-23	1.4103572688826388E-5	3.878482489427257E-4	0.0	6.346607709971875E-4	5.641429075530555E-5
24-25	1.4103572688826388E-5	4.3721075335361804E-4	0.0	7.96851856918691E-4	8.462143613295832E-5
26-27	1.4103572688826388E-5	4.513143260424444E-4	0.0	0.001022509019939913	8.462143613295832E-5
28-29	1.4103572688826388E-5	4.513143260424444E-4	0.0	0.0019392412447136282	8.462143613295832E-5
30-31	1.4103572688826388E-5	5.0772861679775E-4	0.0	0.004308641456436462	8.462143613295832E-5
32-33	1.4103572688826388E-5	5.0772861679775E-4	0.0	0.008723059708039122	1.128285815106111E-4
34-35	1.4103572688826388E-5	6.064536256195348E-4	0.0	0.014724129887134748	1.128285815106111E-4
36-37	1.4103572688826388E-5	6.910750617524931E-4	0.0	0.025337068335476606	1.128285815106111E-4
38-39	1.4103572688826388E-5	6.910750617524931E-4	0.0	0.051097243851618	1.128285815106111E-4
40-41	1.4103572688826388E-5	7.898000705742778E-4	0.0	0.0784581748679412	1.128285815106111E-4
42-43	1.4103572688826388E-5	8.250590022963437E-4	0.0	0.09704668367181438	1.269321541994375E-4
44-45	1.4103572688826388E-5	9.731465155290208E-4	0.0	0.11923865529768268	1.4103572688826388E-4
46-47	1.4103572688826388E-5	0.0010859750970396318	0.0	0.1420088734037929	1.4103572688826388E-4
48-49	1.4103572688826388E-5	0.0010859750970396318	0.0	0.1637565824899632	1.4103572688826388E-4
50-51	1.4103572688826388E-5	0.0011494411741393507	0.0	0.18533504870386758	1.4103572688826388E-4
52-53	1.4103572688826388E-5	0.0011564929604837638	0.0	0.23674257115463976	1.5513929957709026E-4
54-55	1.4103572688826388E-5	0.0011564929604837638	0.0	0.2964641497054751	1.6924287226591664E-4
56-57	1.4103572688826388E-5	0.0011564929604837638	0.0	0.35509270137292637	1.6924287226591664E-4
58-59	1.4103572688826388E-5	0.0011847001058614165	0.0	0.3954571264083475	1.6924287226591664E-4
60-61	1.4103572688826388E-5	0.0012199590375834824	0.0	0.4253284933632818	1.6924287226591664E-4
62-63	1.4103572688826388E-5	0.001241114396616722	0.0	0.4490648061985766	1.7629465861032986E-4
64-65	1.4103572688826388E-5	0.0013680465508161595	0.0	0.4573859140849842	1.8334644495474306E-4
66-67	1.4103572688826388E-5	0.0013680465508161595	0.0	0.46495248083253954	1.9039823129915625E-4
68-69	1.4103572688826388E-5	0.0013821501235049861	0.0	0.47240621899858426	2.0450180398798263E-4
70-71	1.4103572688826388E-5	0.0014033054825382256	0.0	0.47906310530771035	2.256571630212222E-4
72-73	1.4103572688826388E-5	0.001410357268882639	0.0	0.4845070843655973	2.53864308398875E-4
74-75	1.4103572688826388E-5	0.0014385644142602917	0.0	0.488717000813212	2.53864308398875E-4
76-77	1.4103572688826388E-5	0.0014667715596379445	0.0	0.4927717779612496	2.53864308398875E-4
78-79	1.4103572688826388E-5	0.0014808751323267708	0.0	0.49500719423242856	2.53864308398875E-4
80-81	1.4103572688826388E-5	0.0014808751323267708	0.0	0.4957899425166584	2.53864308398875E-4
82-83	1.4103572688826388E-5	0.0014808751323267708	0.0	0.4962624122017341	2.53864308398875E-4
84-85	1.4103572688826388E-5	0.0014808751323267708	0.0	0.49644575864668883	2.53864308398875E-4
86-87	1.4103572688826388E-5	0.0015090822777044236	0.0	0.4966220533052992	2.53864308398875E-4
88	1.4103572688826388E-5	0.0015796001411485555	0.0	0.4966291050916436	2.53864308398875E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8990	0.0	35.75415	1
GTATCAA	14225	0.0	28.840548	1
TCAACGC	15130	0.0	26.187027	4
ATCAACG	15165	0.0	26.017614	3
CAACGCA	15380	0.0	25.707634	5
AACGCAG	15825	0.0	25.036951	6
TATCAAC	17065	0.0	23.532415	2
GTACATG	18940	0.0	21.879002	1
TACATGG	18830	0.0	21.326643	2
ACGCAGA	18965	0.0	20.869844	7
ACATGGG	19050	0.0	20.559847	3
CGCAGAG	19505	0.0	20.376783	8
GGACCGA	1150	0.0	19.400032	6
CATGGGG	15600	0.0	18.035536	4
ACCGAAC	1045	0.0	17.791098	8
GCAGAGT	23105	0.0	17.005175	9
GAGTACT	12405	0.0	16.303614	12-13
AGAGTAC	19515	0.0	16.24873	10-11
CAGAGTA	21445	0.0	16.086813	10-11
AGGACCG	1580	0.0	15.950683	5
>>END_MODULE