FastQCFastQC Report
Fri 27 May 2016
SRR2140279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11701299
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120150.043.4728741
GTATCAA203550.032.2436831
TCAACGC234150.027.5000364
ATCAACG234150.027.4117263
CAACGCA237850.026.9505335
AACGCAG248100.025.9204436
TATCAAC251600.025.6913642
ACGCAGA295100.021.6940447
CGCAGAG300850.021.2794178
GTACATG314950.021.0883561
TACATGG308550.020.8556062
ACATGGG319750.019.5560023
GTGGTAT67350.018.7284161
CATGGGG249050.017.6018434
GAGTACT192650.017.46431212-13
GCAGAGT362150.017.4377069
AGAGTAC319300.016.25501310-11
TGGTATC68550.016.1080362
TATACCG8900.015.7989315
CAGAGTA356500.015.79430810-11
Produced by FastQC (version 0.11.5)