##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11701299
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84727994729474	31.0	31.0	34.0	30.0	34.0
2	31.995812174357734	33.0	31.0	34.0	30.0	34.0
3	31.89018073976231	31.0	31.0	34.0	30.0	34.0
4	35.837819630111156	37.0	35.0	37.0	35.0	37.0
5	35.68472808019007	37.0	35.0	37.0	33.0	37.0
6	35.7443591519198	37.0	35.0	37.0	33.0	37.0
7	35.692677966779584	37.0	35.0	37.0	33.0	37.0
8	35.68246944206793	37.0	35.0	37.0	33.0	37.0
9	37.412288157067	39.0	37.0	39.0	34.0	39.0
10-11	37.27421720443175	39.0	37.0	39.0	34.0	39.0
12-13	37.24553825177871	39.0	37.0	39.0	33.5	39.0
14-15	38.47556553336514	40.0	38.0	41.0	34.0	41.0
16-17	38.30894249433332	40.0	38.0	41.0	33.0	41.0
18-19	38.366789789748985	40.0	38.0	41.0	33.5	41.0
20-21	38.39038886531482	40.0	38.0	41.0	34.0	41.0
22-23	38.30811779203144	40.0	38.0	41.0	34.0	41.0
24-25	38.239448913171685	40.0	38.0	41.0	33.5	41.0
26-27	38.00564083047708	40.0	37.0	41.0	33.0	41.0
28-29	37.87662237010396	40.0	37.0	41.0	33.0	41.0
30-31	37.74156392389416	40.0	37.0	41.0	32.5	41.0
32-33	37.54396500386888	39.5	36.5	41.0	32.0	41.0
34-35	37.650748541156155	40.0	37.0	41.0	32.0	41.0
36-37	37.624960797922455	40.0	37.0	41.0	32.0	41.0
38-39	37.574727996623054	40.0	37.0	41.0	32.0	41.0
40-41	37.49316490608845	40.0	36.5	41.0	32.0	41.0
42-43	37.55938596319938	40.0	37.0	41.0	32.0	41.0
44-45	37.55956613001236	40.0	37.0	41.0	32.0	41.0
46-47	37.48621588683058	40.0	36.5	41.0	32.0	41.0
48-49	37.38316764180459	39.5	36.0	41.0	31.0	41.0
50-51	37.255706835991276	39.0	36.0	41.0	31.0	41.0
52-53	37.058142002489774	39.0	35.5	41.0	31.0	41.0
54-55	36.89491269004981	39.0	35.0	40.0	31.0	41.0
56-57	36.74419828199082	39.0	35.0	40.0	30.5	41.0
58-59	36.56026853183411	38.0	35.0	40.0	30.0	41.0
60-61	36.358498515017985	38.0	35.0	40.0	30.0	41.0
62-63	36.13794300880154	38.0	34.5	40.0	29.5	41.0
64-65	35.82062607364452	37.0	34.0	40.0	29.0	41.0
66-67	35.51661157103244	37.0	34.0	39.5	29.0	41.0
68-69	35.19249117495199	36.0	34.0	39.0	28.5	41.0
70-71	34.87506722098146	36.0	33.5	39.0	28.0	40.0
72-73	34.59465058861518	35.5	33.0	38.5	28.0	40.0
74-75	34.26133265883337	35.0	33.0	37.5	28.0	40.0
76-77	33.039963439833286	34.0	31.5	36.0	26.5	39.0
78-79	33.79910839198711	35.0	32.5	37.0	28.0	39.0
80-81	33.73509200891553	35.0	33.0	36.0	28.5	39.0
82-83	33.515626897403635	35.0	33.0	36.0	28.5	37.5
84-85	33.28826335758147	35.0	33.0	35.5	28.0	37.0
86-87	33.09631365071006	35.0	32.0	35.0	28.0	37.0
88-89	32.93248673653868	35.0	32.0	35.0	28.0	36.0
90-91	32.810718576813656	34.0	32.0	35.0	27.5	36.0
92-93	32.767216632923905	34.0	32.0	35.0	28.0	36.0
94-95	32.70957824667659	34.0	32.0	35.0	27.5	35.0
96-97	32.77456584846557	34.0	32.0	35.0	29.0	35.0
98-99	32.93258459735773	34.0	32.0	35.0	29.0	35.0
100	31.578587949191146	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	82.0
21	534.0
22	2619.0
23	8753.0
24	22327.0
25	47477.0
26	84012.0
27	131443.0
28	185942.0
29	244549.0
30	306143.0
31	374582.0
32	458302.0
33	571787.0
34	747122.0
35	1011851.0
36	1507662.0
37	2328798.0
38	2484504.0
39	1151266.0
40	31533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27635743689654	20.5210207858119	14.010265014166375	24.19235676312519
2	15.231360210520217	21.306489134240568	40.2013571313749	23.26079352386432
3	18.498585237466365	25.648432471827597	30.50170592783322	25.35127636287281
4	13.006923419357117	17.617761925406743	38.21537249838672	31.159942156849425
5	13.251486010228437	37.84508882304435	35.719205192517514	13.184219974209702
6	30.995481783689144	37.59854354631909	18.17888766025037	13.227087009741398
7	27.216747473934305	32.34561393568355	22.75623415827593	17.681404432106213
8	24.962083269558363	36.701950783413025	20.76087449778012	17.575091449248497
9	25.618557392645037	16.566972607058414	21.0721561768484	36.74231382344815
10-11	24.680135940462677	26.61597229504177	28.596401989215043	20.107489775280506
12-13	25.08593703998163	23.970697612290735	28.907897319776204	22.035468027951428
14-15	22.122129346493924	25.530357783353796	26.337135731682437	26.010377138469842
16-17	22.026877868004604	28.69573889865974	27.027096435545843	22.250286797789816
18-19	21.96583984010778	28.031609070153856	28.599553435373505	21.402997654364857
20-21	22.687135732036975	27.201014872642876	28.4744856934092	21.637363701910946
22-23	22.47139534116433	27.124440074199196	28.272032609080288	22.13213197555618
24-25	22.261049053727252	27.218270525203987	28.388220874547155	22.132459546521606
26-27	22.26253382230788	27.384875835880436	28.27292581462253	22.07966452718916
28-29	22.18019793139931	27.531180468145642	28.21795610253383	22.070665497921215
30-31	22.425006534145282	27.394399808743337	28.15254502386249	22.028048633248886
32-33	22.20268534568681	27.47218005694624	28.16076463905628	22.164369958310672
34-35	22.33811561227706	27.49394911470993	28.173604065296885	21.99433120771612
36-37	22.351800026519054	27.46102962188984	28.086815088283533	22.10035526330757
38-39	22.224681632220268	27.54095016110667	28.140928043921388	22.093440162751676
40-41	22.45274263788326	27.423875751095515	28.039126121712716	22.08425548930851
42-43	22.315283464397123	27.50119811844165	27.902057626957333	22.28146079020389
44-45	22.420665278342515	27.59000530851005	27.77368524798232	22.21564416516511
46-47	22.398174696700224	27.56720839270902	27.581682568530148	22.45293434206061
48-49	22.331382668015152	27.698647989681568	27.58627817624272	22.383691166060558
50-51	22.299966472520612	27.752190745066212	27.535092754318498	22.412750028094678
52-53	22.439776916475928	27.680643889925022	27.381726395061023	22.497852798538023
54-55	22.45540372876897	27.580205979349927	27.47627066222207	22.488119629659035
56-57	22.428605492261333	27.644185377049713	27.48396836117412	22.443240769514833
58-59	22.355570416067827	27.788081112901548	27.44970163304648	22.406646837984148
60-61	22.461288680305028	27.69482214367403	27.479792017972503	22.36409715804844
62-63	22.425133923173778	27.76016497221605	27.41124046370633	22.40346064090384
64-65	22.36491181380374	27.774577303053665	27.37366365033874	22.486847232803857
66-67	22.434206865319062	27.821818128606086	27.207982971060595	22.53599203501426
68-69	22.368135359813543	27.775791082738543	27.33434488154299	22.521728675904924
70-71	22.317476924294056	27.648263879750246	27.370187911910442	22.664071284045253
72-73	22.45723965022043	27.558554398329793	27.302696067739173	22.6815098837106
74-75	22.322263336256096	27.779302600081586	27.273936638649076	22.624497425013242
76-77	22.49277134641085	27.704535886844244	27.16378846914513	22.638904297599773
78-79	22.424833670253914	27.680761947762644	27.15030034655624	22.744104035427203
80-81	22.424019713253614	27.7106291063628	27.213319896403465	22.652031283980126
82-83	22.43771050576972	27.697420266443416	27.35238521969805	22.512484008088812
84-85	22.55652806611728	27.74154849943027	27.217193399090966	22.484730035361483
86-87	22.433367993401678	27.733052353657545	27.34177698066685	22.49180267227393
88-89	22.451913655351706	27.73051462692403	27.309716522133886	22.507855195590377
90-91	22.483109101243237	27.806633293673073	27.35041170771363	22.35984589737006
92-93	22.535730290675197	27.738089659023895	27.36008229548659	22.36609775481432
94-95	22.29735740280042	27.87444539841858	27.433831371134033	22.39436582764697
96-97	22.290194742993194	27.96686982905843	27.59833369359917	22.144601734349205
98-99	23.35351937822545	29.960903595858795	27.71198215113661	18.97359487477914
100	22.370007338894357	31.014332097386532	21.357843239571096	25.257817324148007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2048.0
1	1845.5
2	2464.5
3	3457.0
4	4424.0
5	7070.0
6	9665.5
7	11563.5
8	12850.5
9	12702.0
10	11530.5
11	10306.0
12	10797.0
13	14023.0
14	21685.5
15	32341.0
16	42057.0
17	49037.5
18	52339.0
19	52350.0
20	50624.5
21	48774.5
22	48983.0
23	53025.5
24	61744.5
25	76002.0
26	95872.5
27	117990.5
28	140669.5
29	167553.0
30	193485.5
31	218364.5
32	246341.0
33	272586.5
34	299633.0
35	327450.0
36	355873.0
37	383296.0
38	403573.5
39	418227.0
40	435034.0
41	452207.5
42	464006.5
43	476401.5
44	490902.5
45	502689.0
46	508861.0
47	512656.5
48	514658.5
49	510306.0
50	501673.5
51	489010.5
52	472638.0
53	453386.0
54	430326.5
55	402867.5
56	373419.0
57	341356.5
58	304786.5
59	266056.0
60	223950.0
61	182576.5
62	146613.5
63	115020.0
64	87511.5
65	66461.5
66	50975.0
67	39122.5
68	29562.5
69	21528.0
70	15718.0
71	11408.0
72	8115.0
73	5731.0
74	3919.0
75	2611.5
76	1759.5
77	1182.5
78	793.0
79	539.5
80	369.0
81	252.0
82	176.0
83	114.5
84	77.0
85	56.5
86	43.5
87	36.0
88	31.5
89	26.0
90	19.5
91	15.0
92	10.0
93	6.0
94	3.5
95	2.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.589345080405175E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.443395985351711E-4
18-19	0.003563706901259424
20-21	0.0
22-23	0.0012863832233062553
24-25	5.044209072719626E-4
26-27	0.013514264819717141
28-29	0.007447137202760252
30-31	6.602701636861185E-4
32-33	0.006691520188243301
34-35	0.004515833175969931
36-37	0.0019797724564157002
38-39	0.0014484927360933757
40-41	0.003765516975904046
42-43	0.00838478929719516
44-45	0.00952407143791739
46-47	0.007980876127196317
48-49	0.0074161891219374785
50-51	0.032590835090607034
52-53	0.012129371510252323
54-55	5.688069941645617E-5
56-57	0.006938823709917518
58-59	0.0059975254761715745
60-61	1.2723949463017522E-4
62-63	0.005283232721058768
64-65	0.001975940053480443
66-67	0.02164149992249559
68-69	0.029927330761943996
70-71	0.013370693070601984
72-73	0.010395134950714027
74-75	0.015035478621954224
76-77	0.0022845380257313338
78-79	0.0026799368844844045
80-81	0.0012675143921000976
82-83	0.022359110099558946
84-85	7.516273158449017E-4
86-87	0.008484157338813436
88-89	9.105176715913141E-4
90-91	0.003163868785928452
92-93	0.0012021010774181775
94-95	6.871565076407431E-4
96-97	0.0024849011596678725
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	337.0
20-21	1052.0
22-23	2205.0
24-25	4845.0
26-27	7346.0
28-29	15781.0
30-31	33569.0
32-33	41934.0
34-35	57735.0
36-37	86670.0
38-39	98602.0
40-41	102387.0
42-43	109337.0
44-45	125431.0
46-47	123385.0
48-49	115127.0
50-51	103741.0
52-53	97702.0
54-55	105014.0
56-57	111756.0
58-59	112269.0
60-61	110083.0
62-63	111207.0
64-65	116629.0
66-67	121398.0
68-69	122197.0
70-71	120523.0
72-73	116067.0
74-75	118696.0
76-77	116772.0
78-79	125671.0
80-81	127669.0
82-83	125620.0
84-85	127486.0
86-87	137082.0
88-89	148269.0
90-91	161911.0
92-93	182080.0
94-95	226571.0
96-97	473754.0
98-99	791730.0
100-101	6563659.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.68249752197336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.21218131779571	74.65498142056278
2	7.1792137161538285	12.0154906802352
3	1.9382336534215492	4.865886986983624
4	0.7695642637541992	2.5759623837844012
5	0.34812929883757676	1.456616459365092
6	0.1799935694211993	0.903738685623639
7	0.11217969514478744	0.6571233942678583
8	0.07045096778548507	0.47164103497035836
9	0.043501276053321235	0.3276265882981261
>10	0.14302127659494393	1.8042601107481162
>50	0.002832487570540821	0.15717173176442048
>100	6.455395794807299E-4	0.08444196854796024
>500	5.293788731551493E-5	0.025058554848393644
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7092119430500837E-5
2	0.0	5.127635829150251E-5	0.0	0.0	1.7092119430500837E-5
3	0.0	5.982241800675293E-5	0.0	1.7092119430500837E-5	1.7092119430500837E-5
4	0.0	1.6237513458975793E-4	0.0	2.5638179145751253E-5	1.7092119430500837E-5
5	0.0	1.6237513458975793E-4	0.0	4.273029857625209E-5	1.7092119430500837E-5
6	1.0255271658300501E-4	1.6237513458975793E-4	0.0	5.982241800675293E-5	2.5638179145751253E-5
7	1.0255271658300501E-4	1.7092119430500835E-4	0.0	6.836847772200335E-5	2.5638179145751253E-5
8	1.0255271658300501E-4	2.0510543316601003E-4	0.0	1.0255271658300501E-4	2.5638179145751253E-5
9	1.0255271658300501E-4	3.760266274710184E-4	0.0	1.2819089572875628E-4	5.127635829150251E-5
10-11	1.1109877629825543E-4	3.760266274710184E-4	0.0	1.452830151592571E-4	5.127635829150251E-5
12-13	1.1964483601350585E-4	3.760266274710184E-4	0.0	1.6237513458975793E-4	5.5549388149127714E-5
14-15	1.1964483601350585E-4	5.512208516336519E-4	0.0	1.7946725402025876E-4	1.0682574644063022E-4
16-17	2.0510543316601003E-4	5.640399412065275E-4	0.0	1.9655937345075961E-4	1.1964483601350585E-4
18-19	2.0510543316601003E-4	6.281353890709056E-4	0.0	2.0937846302363525E-4	1.367369554440067E-4
20-21	2.0510543316601003E-4	7.007768966505343E-4	0.0	2.606548213151378E-4	1.367369554440067E-4
22-23	2.0937846302363525E-4	7.819644639454132E-4	0.0	3.332963288947663E-4	1.4955604501688233E-4
24-25	2.1365149288126044E-4	8.631520312402922E-4	0.0	3.589345080405175E-4	1.8374028387788398E-4
26-27	2.1365149288126044E-4	9.1870141938942E-4	0.0	5.597669113489023E-4	1.9228634359313442E-4
28-29	2.1365149288126044E-4	9.315205089622956E-4	0.0	0.0015682019577484517	1.9655937345075961E-4
30-31	2.1365149288126044E-4	0.001063984434548677	0.0	0.004704605873245355	1.9655937345075961E-4
32-33	2.1792452273888563E-4	0.0010896226136944283	0.0	0.010926137345947658	2.0937846302363525E-4
34-35	2.2219755259651085E-4	0.001307547136433314	0.0	0.018544949582093406	2.307436123117613E-4
36-37	2.2219755259651085E-4	0.0015297446890298247	0.0	0.03151359519998592	2.307436123117613E-4
38-39	2.2219755259651085E-4	0.0015895671070365778	0.0	0.06561664649369271	2.307436123117613E-4
40-41	2.2219755259651085E-4	0.0016921198236195826	0.0	0.10215532480624587	2.3501664216938652E-4
42-43	2.2219755259651085E-4	0.0017305770923382096	0.0	0.12605010777008602	2.435627018846369E-4
44-45	2.2219755259651085E-4	0.0019399555553618448	0.0	0.1558203067881609	2.478357317422621E-4
46-47	2.2219755259651085E-4	0.002110876749666853	0.0	0.1861160884787236	2.64927851172763E-4
48-49	2.2647058245413607E-4	0.0021194228093821036	0.0	0.21296353507418278	2.820199706032638E-4
50-51	2.5638179145751256E-4	0.002311709152975238	0.0	0.23884527692181867	2.820199706032638E-4
52-53	2.734739108880134E-4	0.0023245282425481137	0.0	0.3010093152905502	2.905660303185142E-4
54-55	2.734739108880134E-4	0.0023416203619786146	0.0	0.37621464078475386	3.033851198913898E-4
56-57	2.734739108880134E-4	0.0023587124814091155	0.0	0.44820664782602343	3.1193117960664026E-4
58-59	2.734739108880134E-4	0.0023843506605548664	0.0	0.4968764579043745	3.162042094642655E-4
60-61	3.033851198913898E-4	0.002439900048703994	0.0	0.532248599065796	3.2475026917951587E-4
62-63	3.5038844832526714E-4	0.0024740842875649958	0.0	0.560010474050787	3.418423886100167E-4
64-65	3.5038844832526714E-4	0.0027518312283106344	0.0	0.5683386092432985	3.418423886100167E-4
66-67	3.5038844832526714E-4	0.0027774694074563858	0.0	0.5758377766434308	3.461154184676419E-4
68-69	3.5038844832526714E-4	0.0028415648553207643	0.0	0.5830036477146683	3.5038844832526714E-4
70-71	3.5466147818289236E-4	0.0028928412136122665	0.0	0.5891354455603603	3.760266274710184E-4
72-73	3.845726871862688E-4	0.0029142063629003924	0.0	0.5938528705231787	4.5294116490827215E-4
74-75	3.845726871862688E-4	0.0029996669600528968	0.0	0.5975404952903092	4.5294116490827215E-4
76-77	3.845726871862688E-4	0.0031022196766359016	0.0	0.6007281755640976	4.5721419476589737E-4
78-79	3.845726871862688E-4	0.0031534960349274043	0.0	0.6030740689559339	4.614872246235226E-4
80-81	3.845726871862688E-4	0.0031534960349274043	0.0	0.6038859446288827	4.657602544811478E-4
82-83	3.845726871862688E-4	0.00316631512450028	0.0	0.604531172137384	4.956714634845242E-4
84-85	3.88845717043894E-4	0.003209045423076532	0.0	0.604749096660123	4.956714634845242E-4
86-87	3.9311874690151923E-4	0.0032389566320799082	0.0	0.6048901066454246	4.956714634845242E-4
88	3.9311874690151923E-4	0.003315871169517162	0.0	0.6048986527051399	4.956714634845242E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12015	0.0	43.472874	1
GTATCAA	20355	0.0	32.243683	1
TCAACGC	23415	0.0	27.500036	4
ATCAACG	23415	0.0	27.411726	3
CAACGCA	23785	0.0	26.950533	5
AACGCAG	24810	0.0	25.920443	6
TATCAAC	25160	0.0	25.691364	2
ACGCAGA	29510	0.0	21.694044	7
CGCAGAG	30085	0.0	21.279417	8
GTACATG	31495	0.0	21.088356	1
TACATGG	30855	0.0	20.855606	2
ACATGGG	31975	0.0	19.556002	3
GTGGTAT	6735	0.0	18.728416	1
CATGGGG	24905	0.0	17.601843	4
GAGTACT	19265	0.0	17.464312	12-13
GCAGAGT	36215	0.0	17.437706	9
AGAGTAC	31930	0.0	16.255013	10-11
TGGTATC	6855	0.0	16.108036	2
TATACCG	890	0.0	15.798931	5
CAGAGTA	35650	0.0	15.794308	10-11
>>END_MODULE