FastQCFastQC Report
Fri 27 May 2016
SRR2140278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7138908
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81800.042.9735531
GTATCAA142400.033.2019121
ATCAACG165650.027.8245623
TCAACGC166700.027.5755714
CAACGCA168350.027.329645
AACGCAG174150.026.5841166
TATCAAC176200.026.367832
ACGCAGA206250.022.3274757
CGCAGAG210400.021.8676098
TACATGG206800.020.6435532
GTACATG212650.020.6343771
ACATGGG209650.019.7961983
CGTCGAA3601.0461337E-418.8747294
GTGGTAT48050.018.7584321
GCAGAGT245900.018.4773739
CATGGGG158800.017.440684
GAGTACT136150.017.4401512-13
GGACCGA11700.016.8082566
TACACCG8300.016.7829425
AGAGTAC220200.016.72673210-11
Produced by FastQC (version 0.11.5)