##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7138908
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06800886073893	33.0	31.0	34.0	30.0	34.0
2	32.20229746622312	33.0	31.0	34.0	30.0	34.0
3	32.106441069138306	33.0	31.0	34.0	30.0	34.0
4	35.98751013460322	37.0	35.0	37.0	35.0	37.0
5	35.86082479281145	37.0	35.0	37.0	35.0	37.0
6	35.9168061838029	37.0	35.0	37.0	35.0	37.0
7	35.86937343918706	37.0	35.0	37.0	35.0	37.0
8	35.861440293109254	37.0	35.0	37.0	35.0	37.0
9	37.616166085905576	39.0	37.0	39.0	35.0	39.0
10-11	37.50067286201195	39.0	37.0	39.0	35.0	39.0
12-13	37.47584399462775	39.0	37.0	39.0	34.5	39.0
14-15	38.77653788226435	40.0	38.0	41.0	35.0	41.0
16-17	38.62618883728436	40.0	38.0	41.0	34.0	41.0
18-19	38.6964743067147	40.0	38.0	41.0	34.0	41.0
20-21	38.725345572708854	40.0	38.0	41.0	34.0	41.0
22-23	38.65357015183075	40.0	38.0	41.0	34.0	41.0
24-25	38.60180065086627	40.0	38.0	41.0	34.0	41.0
26-27	38.402585026863505	40.0	38.0	41.0	34.0	41.0
28-29	38.294285292483366	40.0	38.0	41.0	34.0	41.0
30-31	38.175961200755324	40.0	38.0	41.0	33.5	41.0
32-33	37.99542936757786	40.0	37.0	41.0	33.0	41.0
34-35	38.082620213669756	40.0	37.5	41.0	33.0	41.0
36-37	38.05839155924278	40.0	37.0	41.0	33.0	41.0
38-39	38.01222634656712	40.0	37.0	41.0	33.0	41.0
40-41	37.948091675960924	40.0	37.0	41.0	33.0	41.0
42-43	38.01088703502087	40.0	37.0	41.0	33.0	41.0
44-45	38.017104795172	40.0	37.0	41.0	33.0	41.0
46-47	37.95729786957568	40.0	37.0	41.0	33.0	41.0
48-49	37.87106290890164	40.0	37.0	41.0	33.0	41.0
50-51	37.74792630303536	40.0	37.0	41.0	33.0	41.0
52-53	37.576269294568014	40.0	36.0	41.0	32.0	41.0
54-55	37.42727309131757	39.0	36.0	41.0	32.0	41.0
56-57	37.28741105037321	39.0	36.0	41.0	31.5	41.0
58-59	37.11817524383896	39.0	35.0	41.0	31.0	41.0
60-61	36.92802586448888	39.0	35.0	40.5	31.0	41.0
62-63	36.71855398141466	38.0	35.0	40.0	31.0	41.0
64-65	36.427789339253536	38.0	35.0	40.0	31.0	41.0
66-67	36.135244712638745	37.5	34.5	40.0	30.0	41.0
68-69	35.82779686127694	37.0	34.0	39.5	30.0	41.0
70-71	35.52523185564475	36.5	34.0	39.0	30.0	41.0
72-73	35.24220675585485	36.0	34.0	39.0	29.0	40.5
74-75	34.9135351036816	35.5	34.0	38.5	29.0	40.0
76-77	33.63841202973239	34.5	32.0	36.5	28.0	39.0
78-79	34.332821259851855	35.0	33.5	37.0	29.0	39.0
80-81	34.24177519233921	35.0	34.0	37.0	29.0	39.0
82-83	34.008154072292015	35.0	33.5	36.0	29.0	38.5
84-85	33.769254084169276	35.0	33.0	36.0	29.0	37.0
86-87	33.56342076479365	35.0	33.0	35.5	29.0	37.0
88-89	33.39612869757501	35.0	33.0	35.0	29.0	36.5
90-91	33.270049831187606	35.0	33.0	35.0	29.0	36.0
92-93	33.215756795459384	35.0	33.0	35.0	29.0	36.0
94-95	33.140313956193864	35.0	33.0	35.0	29.0	36.0
96-97	33.17622843281843	35.0	33.0	35.0	29.0	35.0
98-99	33.29438085197164	35.0	33.0	35.0	29.0	35.0
100	32.124562083058635	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	40.0
21	225.0
22	1100.0
23	3665.0
24	9751.0
25	20593.0
26	38000.0
27	60822.0
28	87779.0
29	117799.0
30	150434.0
31	187302.0
32	233432.0
33	295079.0
34	391899.0
35	538689.0
36	824934.0
37	1384242.0
38	1752136.0
39	999085.0
40	41894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.681052620372746	20.395500264186065	13.749651907546646	24.173795207894543
2	15.061112988148887	21.133288172364736	40.639576809226284	23.166022030260088
3	18.47709076300248	25.760450960926836	30.41285555777657	25.349602718294122
4	12.738740995121384	17.616587859095535	38.499725728360694	31.144945417422388
5	13.102606168898662	37.896748354230084	35.82550720642429	13.175138270446965
6	30.58173603021639	37.700107635509525	18.342259068193624	13.37589726608047
7	27.00858450620179	32.3974479009955	22.817747476224657	17.776220116578052
8	24.79615930055409	36.68306133094866	20.976163301165947	17.544616067331305
9	25.492988563517	16.59984972491591	21.33210009149859	36.5750616200685
10-11	24.553426098221184	26.710555732053137	28.63706185876047	20.098956310965207
12-13	25.0244435143302	24.075551891129567	29.012833615449306	21.887170979090918
14-15	22.016777636019402	25.66173986273531	26.509124364678744	25.812358136566544
16-17	21.8175079114794	28.894236321876303	27.151136904611405	22.13711886203289
18-19	21.738033830134622	28.20747046126672	28.793268685591244	21.261227023007407
20-21	22.502626388673026	27.288940016217456	28.713343063059455	21.495090532050067
22-23	22.241946813932824	27.309164224948702	28.477749380966006	21.971139580152467
24-25	22.04914742524955	27.389250693712587	28.656371397075457	21.905230483962406
26-27	22.05386743904654	27.627265763040725	28.466180068653546	21.85268672925918
28-29	21.89137091295924	27.803060929667545	28.474136369332214	21.831431788040998
30-31	22.22675978026346	27.52009580098097	28.439144632893314	21.813999785862254
32-33	21.934362292075228	27.642969687298187	28.457744273995434	21.96492374663115
34-35	22.068679927569885	27.66565228414634	28.471824914922113	21.793842873361665
36-37	22.06353359621612	27.742556295833065	28.3132006424946	21.880709465456217
38-39	21.91176915050552	27.712999676177454	28.45415752167812	21.921073651638903
40-41	22.14563316275026	27.568318800016073	28.349557728885483	21.936490308348176
42-43	22.087446377494945	27.668181034907462	28.15843135170306	22.08594123589453
44-45	22.126370164058056	27.826231853731148	27.995826482543983	22.05157149966681
46-47	22.181477308343165	27.71636045567997	27.7956704146707	22.306491821306174
48-49	22.13035223094723	27.83440990624704	27.780247583096727	22.254990279709006
50-51	22.055459167725118	27.904868612678307	27.752040271827287	22.287631947769288
52-53	22.15738025164544	27.848573750171017	27.705172141431618	22.288873856751927
54-55	22.201092349390272	27.793172337058564	27.675936242797665	22.329799070753502
56-57	22.244801879788266	27.884603546303616	27.597864665507448	22.272729908400674
58-59	22.115493629295063	28.027148484313546	27.697165308891584	22.160192577499803
60-61	22.248756196255187	27.982874990864097	27.636544030006245	22.13182478287447
62-63	22.142532444487358	28.09583093755441	27.5372110970259	22.22442552093233
64-65	22.08266117539463	28.08552636780066	27.566737637668414	22.26507481913629
66-67	22.181638260094093	28.113171683983428	27.368103410853823	22.337086645068652
68-69	22.146652462850792	28.033597172174236	27.511682103294493	22.308068261680482
70-71	22.12932245370062	27.85567429905677	27.551092805726636	22.463910441515974
72-73	22.24996788916795	27.848993051618976	27.530355469101142	22.37068359011193
74-75	22.141795806296898	27.9964617975165	27.477537851750135	22.38420454443647
76-77	22.291541965380887	27.911165738158555	27.329620618269274	22.46767167819128
78-79	22.192185185475036	27.89148920022575	27.367302616264627	22.549022998034584
80-81	22.148151380683213	27.967963089889516	27.456470797286762	22.42741473214051
82-83	22.215188742097748	27.93397271660652	27.554482981031786	22.296355560263954
84-85	22.409678168161854	27.927584113037433	27.411205939496842	22.251531779303875
86-87	22.18443261907262	28.023766835090154	27.502442908884912	22.289357636952317
88-89	22.251526259165033	27.9504891243465	27.52182471915209	22.27615989733638
90-91	22.335641409598512	27.9955767975151	27.464003067846065	22.20477872504032
92-93	22.30678058160708	27.944168459183928	27.537663117889284	22.21138784131971
94-95	22.083959137569355	28.027578830645993	27.68059365548287	22.207868376301775
96-97	22.047313766079053	28.19807993127507	27.77131754262806	21.983288760017818
98-99	23.207306111584362	30.1141547787343	27.92578390446962	18.75275520521172
100	29.623116177822467	41.515421441580784	28.86146238059675	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1149.0
1	1079.5
2	1613.0
3	2320.0
4	3179.5
5	5348.5
6	7168.0
7	8190.5
8	8700.5
9	8432.0
10	7636.5
11	6672.5
12	6977.0
13	9391.5
14	15052.5
15	22838.0
16	29488.0
17	33975.0
18	35974.5
19	35635.0
20	33950.0
21	32092.5
22	31844.5
23	34329.5
24	40020.5
25	49140.0
26	61704.5
27	75888.0
28	90822.0
29	108549.0
30	126064.5
31	143093.0
32	161085.0
33	178729.0
34	195866.5
35	212247.5
36	229017.5
37	244487.5
38	257068.0
39	265837.0
40	274220.0
41	283638.5
42	290959.0
43	298494.0
44	307371.5
45	314095.5
46	317440.5
47	319843.5
48	318914.5
49	314304.5
50	308167.5
51	297306.0
52	283536.5
53	270096.5
54	254851.0
55	236250.0
56	216090.5
57	195023.5
58	172661.5
59	148715.0
60	124125.0
61	101228.5
62	81292.5
63	63780.0
64	48860.5
65	37644.0
66	28879.5
67	22244.0
68	16975.0
69	12524.5
70	9071.0
71	6560.0
72	4801.5
73	3387.0
74	2297.5
75	1561.5
76	1066.0
77	680.0
78	438.0
79	304.5
80	216.5
81	150.0
82	110.5
83	77.5
84	52.5
85	37.0
86	27.0
87	22.0
88	17.5
89	12.5
90	9.0
91	6.0
92	3.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.6614714743487377E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0010155614836330712
18-19	0.0038171104040001635
20-21	0.0
22-23	0.0014990527247664945
24-25	6.095763178339329E-4
26-27	0.01380227708831817
28-29	0.0075909177265187375
30-31	7.662782456136898E-4
32-33	0.006899132148432078
34-35	0.004903261902065988
36-37	0.0022963027884889053
38-39	0.0016478697972738766
40-41	0.0036480031579208213
42-43	0.009030034113706923
44-45	0.010149845729027334
46-47	0.009616732964980128
48-49	0.008258786682999527
50-51	0.03481225363613681
52-53	0.013384017880612383
54-55	7.85658773310103E-5
56-57	0.008966984342009201
58-59	0.006491893850143791
60-61	1.6259643086200578E-4
62-63	0.005608912875078572
64-65	0.0023466897984817585
66-67	0.0255418995016001
68-69	0.03366748935563431
70-71	0.016627131811111633
72-73	0.011747767289082242
74-75	0.01724984138127861
76-77	0.002254452611271383
78-79	0.0029301101393822454
80-81	0.0018359505315584212
82-83	0.02574813440638434
84-85	9.201642711407266E-4
86-87	0.010171937500998427
88-89	9.775157687956244E-4
90-91	0.003270962995366964
92-93	0.0012153083890611308
94-95	7.137220511875249E-4
96-97	0.0031409917740087296
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	189.0
20-21	645.0
22-23	1260.0
24-25	3007.0
26-27	4654.0
28-29	11299.0
30-31	23422.0
32-33	28984.0
34-35	40126.0
36-37	60112.0
38-39	67930.0
40-41	69044.0
42-43	73774.0
44-45	83392.0
46-47	82888.0
48-49	75861.0
50-51	67203.0
52-53	64247.0
54-55	67817.0
56-57	71594.0
58-59	72793.0
60-61	71457.0
62-63	70741.0
64-65	73518.0
66-67	77588.0
68-69	77052.0
70-71	73378.0
72-73	69772.0
74-75	70762.0
76-77	71289.0
78-79	74358.0
80-81	76167.0
82-83	73332.0
84-85	74173.0
86-87	79349.0
88-89	84401.0
90-91	90166.0
92-93	99578.0
94-95	120491.0
96-97	263430.0
98-99	1462253.0
100-101	3015412.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.21276901023373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49220700686874	76.25878763892327
2	7.313192111877964	12.463547003138407
3	1.8497120566589635	4.72857258658574
4	0.6539476765863772	2.22898769238936
5	0.2904362345951265	1.2374437885378287
6	0.15284800028238427	0.7814760805043369
7	0.08112014981809378	0.4838730811967345
8	0.043169238429143574	0.2942856274088253
9	0.03102527577125732	0.23793746919974565
>10	0.09079602505484127	1.1340496855783766
>50	0.001210432010706549	0.06702983593963076
>100	2.8537934528671344E-4	0.053745576923992
>500	5.0412701147663507E-5	0.030263933673739068
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4007744601835463E-5
2	0.0	4.202323380550639E-5	0.0	0.0	1.4007744601835463E-5
3	0.0	4.202323380550639E-5	0.0	2.8015489203670925E-5	1.4007744601835463E-5
4	0.0	1.2606970141651916E-4	0.0	2.8015489203670925E-5	1.4007744601835463E-5
5	0.0	1.2606970141651916E-4	0.0	2.8015489203670925E-5	1.4007744601835463E-5
6	4.202323380550639E-5	1.2606970141651916E-4	0.0	2.8015489203670925E-5	2.8015489203670925E-5
7	4.202323380550639E-5	1.2606970141651916E-4	0.0	4.202323380550639E-5	4.202323380550639E-5
8	4.202323380550639E-5	1.2606970141651916E-4	0.0	4.202323380550639E-5	4.202323380550639E-5
9	4.202323380550639E-5	2.6614714743487377E-4	0.0	8.404646761101278E-5	5.603097840734185E-5
10-11	4.902710610642412E-5	2.6614714743487377E-4	0.0	8.404646761101278E-5	5.603097840734185E-5
12-13	7.003872300917732E-5	2.6614714743487377E-4	0.0	1.0505808451376596E-4	8.404646761101278E-5
14-15	7.003872300917732E-5	3.151742535412979E-4	0.0	1.120619568146837E-4	1.4007744601835464E-4
16-17	1.1906582911560144E-4	3.2217812584221567E-4	0.0	1.1906582911560144E-4	1.4007744601835464E-4
18-19	1.2606970141651916E-4	3.7120523194863976E-4	0.0	1.2606970141651916E-4	1.8210067982386103E-4
20-21	1.4007744601835464E-4	4.202323380550639E-4	0.0	1.6809293522202553E-4	2.6614714743487377E-4
22-23	1.4007744601835464E-4	4.6225557186057025E-4	0.0	2.1712004132844968E-4	2.6614714743487377E-4
24-25	1.4708131831927236E-4	5.182865502679121E-4	0.0	3.2217812584221567E-4	2.6614714743487377E-4
26-27	1.5408519062019008E-4	5.46302039471583E-4	0.0	4.1322846575414616E-4	2.6614714743487377E-4
28-29	1.5408519062019008E-4	5.46302039471583E-4	0.0	0.0012326815249615207	2.8015489203670927E-4
30-31	1.6809293522202556E-4	5.603097840734185E-4	0.0	0.004237342742055228	3.0116650893946247E-4
32-33	1.6809293522202556E-4	5.603097840734185E-4	0.0	0.00910503399119305	3.0817038124038017E-4
34-35	1.7509680752294328E-4	6.583639962862667E-4	0.0	0.015226418382195147	3.0817038124038017E-4
36-37	1.8910455212477873E-4	7.56418208499115E-4	0.0	0.025459075813835955	3.0817038124038017E-4
38-39	1.9610842442569648E-4	7.56418208499115E-4	0.0	0.05517650598662989	3.0817038124038017E-4
40-41	1.9610842442569648E-4	8.544724207119632E-4	0.0	0.08728926048633769	3.0817038124038017E-4
42-43	2.0311229672661423E-4	9.034995268183874E-4	0.0	0.10600360727438987	3.151742535412979E-4
44-45	2.1011616902753195E-4	0.0010575847174385774	0.0	0.1285700838279468	3.2217812584221567E-4
46-47	2.1011616902753195E-4	0.0011486350573505079	0.0	0.15159181208106337	3.361858704440511E-4
48-49	2.1011616902753195E-4	0.0011486350573505079	0.0	0.17330381621390833	3.361858704440511E-4
50-51	2.3112778593028512E-4	0.0013097241202716158	0.0	0.19401426660772209	3.5019361504588656E-4
52-53	2.3813165823120287E-4	0.0013167279925725335	0.0	0.2502343495671887	3.5019361504588656E-4
54-55	2.3813165823120287E-4	0.0013167279925725335	0.0	0.31705129131794385	3.5019361504588656E-4
56-57	2.3813165823120287E-4	0.0013447434817762045	0.0	0.3802682427060273	3.6420135964772206E-4
58-59	2.3813165823120287E-4	0.0013447434817762045	0.0	0.41836930802301975	3.782091042495575E-4
60-61	2.3813165823120287E-4	0.0014077783324844642	0.0	0.4436953102631383	3.782091042495575E-4
62-63	2.6614714743487377E-4	0.0014287899493872172	0.0	0.4623746376896859	3.9221684885139296E-4
64-65	2.6614714743487377E-4	0.0016248983738129136	0.0	0.47040807921883854	4.062245934532284E-4
66-67	2.731510197357915E-4	0.0016319022461138313	0.0	0.47711778888311773	4.062245934532284E-4
68-69	2.8015489203670927E-4	0.0016599177353175023	0.0	0.4837784714412905	4.2023233805506385E-4
70-71	3.0116650893946247E-4	0.00166692160761842	0.0	0.4897877938754779	4.3424008265689935E-4
72-73	3.361858704440511E-4	0.001673925479919338	0.0	0.49476054320912943	4.3424008265689935E-4
74-75	3.361858704440511E-4	0.0017369603306275974	0.0	0.4990259014403884	4.482478272587348E-4
76-77	3.571974873468043E-4	0.0018069990536367748	0.0	0.5030951512472216	4.6225557186057025E-4
78-79	3.782091042495575E-4	0.001849022287442281	0.0	0.5051753013205942	4.6225557186057025E-4
80-81	3.782091042495575E-4	0.001849022287442281	0.0	0.5059667388905978	4.902710610642411E-4
82-83	3.782091042495575E-4	0.001849022287442281	0.0	0.5063869712286528	5.182865502679121E-4
84-85	3.782091042495575E-4	0.001849022287442281	0.0	0.5065970873976804	5.392981671706653E-4
86-87	3.8521297655047526E-4	0.001877037776645952	0.0	0.5066531183760878	5.673136563743362E-4
88	3.9221684885139296E-4	0.0019330687550532938	0.0	0.5066601222483887	5.743175286752539E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8180	0.0	42.973553	1
GTATCAA	14240	0.0	33.201912	1
ATCAACG	16565	0.0	27.824562	3
TCAACGC	16670	0.0	27.575571	4
CAACGCA	16835	0.0	27.32964	5
AACGCAG	17415	0.0	26.584116	6
TATCAAC	17620	0.0	26.36783	2
ACGCAGA	20625	0.0	22.327475	7
CGCAGAG	21040	0.0	21.867609	8
TACATGG	20680	0.0	20.643553	2
GTACATG	21265	0.0	20.634377	1
ACATGGG	20965	0.0	19.796198	3
CGTCGAA	360	1.0461337E-4	18.87472	94
GTGGTAT	4805	0.0	18.758432	1
GCAGAGT	24590	0.0	18.477373	9
CATGGGG	15880	0.0	17.44068	4
GAGTACT	13615	0.0	17.44015	12-13
GGACCGA	1170	0.0	16.808256	6
TACACCG	830	0.0	16.782942	5
AGAGTAC	22020	0.0	16.726732	10-11
>>END_MODULE