FastQCFastQC Report
Fri 27 May 2016
SRR2140277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7033985
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69350.043.2685431
GTATCAA120250.031.913481
TCAACGC137400.027.1575664
ATCAACG138150.027.0692353
CAACGCA141400.026.3027045
TATCAAC147300.025.748052
AACGCAG147650.025.2169656
ACGCAGA171650.021.6911437
TACATGG180000.021.1612132
GTACATG186700.020.9266171
CGCAGAG178650.020.7955198
ACATGGG185950.019.8693563
CATGGGG143300.018.4612124
GCAGAGT209100.017.6109969
GAGTACT114650.017.20100612-13
GTGGTAT44150.016.7370641
TATACCG4151.8189894E-1216.7236945
CAGAGTA202050.016.2052110-11
AGAGTAC186200.015.89635110-11
TGGTATC43000.015.6655942
Produced by FastQC (version 0.11.5)