##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7033985
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06289251398745	33.0	31.0	34.0	30.0	34.0
2	32.20074054749904	33.0	31.0	34.0	30.0	34.0
3	32.10584782310454	33.0	31.0	34.0	30.0	34.0
4	35.98377519997555	37.0	35.0	37.0	35.0	37.0
5	35.856527843036346	37.0	35.0	37.0	35.0	37.0
6	35.91027859740958	37.0	35.0	37.0	35.0	37.0
7	35.864712534928636	37.0	35.0	37.0	35.0	37.0
8	35.855396763001345	37.0	35.0	37.0	35.0	37.0
9	37.61180269790169	39.0	37.0	39.0	35.0	39.0
10-11	37.49698385765679	39.0	37.0	39.0	35.0	39.0
12-13	37.474436965674506	39.0	37.0	39.0	34.5	39.0
14-15	38.78000727894643	40.0	38.0	41.0	35.0	41.0
16-17	38.63069732164627	40.0	38.0	41.0	34.0	41.0
18-19	38.698210687114056	40.0	38.0	41.0	34.0	41.0
20-21	38.724828547890354	40.0	38.0	41.0	34.0	41.0
22-23	38.65411481265443	40.0	38.0	41.0	34.0	41.0
24-25	38.60132636138188	40.0	38.0	41.0	34.0	41.0
26-27	38.40445051666391	40.0	38.0	41.0	34.0	41.0
28-29	38.301109976031114	40.0	38.0	41.0	34.0	41.0
30-31	38.185883374521964	40.0	38.0	41.0	33.5	41.0
32-33	38.01138655044746	40.0	37.0	41.0	33.0	41.0
34-35	38.09806378903039	40.0	38.0	41.0	33.0	41.0
36-37	38.07505135699826	40.0	37.5	41.0	33.0	41.0
38-39	38.03064670747999	40.0	37.0	41.0	33.0	41.0
40-41	37.96417252535129	40.0	37.0	41.0	33.0	41.0
42-43	38.02645997414287	40.0	37.0	41.0	33.0	41.0
44-45	38.03341592661939	40.0	37.0	41.0	33.0	41.0
46-47	37.974846623189976	40.0	37.0	41.0	33.0	41.0
48-49	37.88457100540608	40.0	37.0	41.0	33.0	41.0
50-51	37.76564004238189	40.0	37.0	41.0	33.0	41.0
52-53	37.58924704807271	40.0	36.5	41.0	32.0	41.0
54-55	37.43835358053221	39.0	36.0	41.0	32.0	41.0
56-57	37.298293910161064	39.0	36.0	41.0	31.5	41.0
58-59	37.12458144567832	39.0	35.0	41.0	31.0	41.0
60-61	36.93314744449057	39.0	35.0	40.5	31.0	41.0
62-63	36.7210935478127	38.0	35.0	40.0	31.0	41.0
64-65	36.41903953298759	38.0	35.0	40.0	31.0	41.0
66-67	36.123041372861266	37.0	34.5	40.0	30.0	41.0
68-69	35.80798870608708	37.0	34.0	39.5	30.0	41.0
70-71	35.49589870892254	36.0	34.0	39.0	30.0	41.0
72-73	35.20563193612617	36.0	34.0	39.0	29.0	40.5
74-75	34.874989933994854	35.5	34.0	38.5	29.0	40.0
76-77	33.59800157468207	34.5	32.0	36.5	28.0	39.0
78-79	34.283917033793855	35.0	33.5	37.0	29.0	39.0
80-81	34.19539979537682	35.0	34.0	36.5	29.0	39.0
82-83	33.968858187153714	35.0	33.5	36.0	29.0	38.0
84-85	33.736722189056735	35.0	33.0	36.0	29.0	37.0
86-87	33.54232488678173	35.0	33.0	35.0	29.0	37.0
88-89	33.38060420735106	35.0	33.0	35.0	29.0	36.5
90-91	33.25688876594387	35.0	33.0	35.0	29.0	36.0
92-93	33.207907047681005	35.0	33.0	35.0	29.0	36.0
94-95	33.14011844500435	35.0	33.0	35.0	29.0	36.0
96-97	33.17827823731141	35.0	33.0	35.0	29.0	35.0
98-99	33.294265434996944	35.0	33.0	35.0	29.0	35.0
100	32.11467509239262	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	8.0
20	36.0
21	220.0
22	1026.0
23	3648.0
24	9739.0
25	21238.0
26	38065.0
27	60890.0
28	87114.0
29	116100.0
30	147439.0
31	183778.0
32	227594.0
33	286473.0
34	380548.0
35	524443.0
36	806520.0
37	1367842.0
38	1737122.0
39	991088.0
40	43049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60018822900532	20.255943110484314	13.70942644887642	24.434442211633947
2	15.122267107478905	21.14715911393044	40.385542476988505	23.345031301602152
3	18.47922869019529	25.542905650404172	30.386239210731596	25.591626448668936
4	12.819418864271107	17.584982054980212	38.31832169104711	31.27727738970157
5	13.312823385321407	37.73573585954477	35.68514860352987	13.266292151603961
6	30.80199915126347	37.53468339781788	18.333917402439724	13.32940004847892
7	27.192125089831727	32.23777133445693	22.779064783334054	17.791038792377297
8	24.889902381082702	36.55577883660543	20.966834589496568	17.58748419281531
9	25.579454605035412	16.640638272615025	21.150130402609616	36.62977671973995
10-11	24.63278639348819	26.63966442919625	28.603515645825233	20.12403353149033
12-13	25.0271076779379	23.95811904631585	28.901156314663734	22.113616961082517
14-15	22.106451748191102	25.522815303131868	26.326001548197787	26.044731400479243
16-17	22.010654960322903	28.757772884969903	26.99511807591428	22.23645407879291
18-19	21.98460643213254	28.04948649483623	28.528976926424537	21.436930146606695
20-21	22.702256589939978	27.222616157210982	28.392862537777773	21.682264715071263
22-23	22.473405484339043	27.183679360039587	28.18135671664817	22.161558438973202
24-25	22.261888813324447	27.278289236219848	28.33254118664995	22.12728076380575
26-27	22.265998952754426	27.49583805257856	28.166772434675213	22.071390559991805
28-29	22.153767200143083	27.609682773484977	28.176351946340223	22.060198080031714
30-31	22.42775695535444	27.405864993935424	28.123947296067755	22.042430754642382
32-33	22.22274724417172	27.490590771402818	28.106906878527344	22.17975510589812
34-35	22.306506704499757	27.514237748977223	28.1173320538201	22.06192349270292
36-37	22.34594241717948	27.48415958821698	28.0491856124099	22.120712382193645
38-39	22.169951172609903	27.54566928155862	28.134798081974193	22.149581463857285
40-41	22.421299589966733	27.44287873465327	27.998047249766056	22.137774425613944
42-43	22.34205809528363	27.502157590193832	27.87703492398443	22.278749390538106
44-45	22.352674121389114	27.641043469966352	27.723216620393178	22.28306578825136
46-47	22.392705243944913	27.51059775853164	27.584934196504445	22.511762801019
48-49	22.342616781117925	27.659237302142415	27.554611267639434	22.44353464910023
50-51	22.271709181009157	27.726907935113047	27.55404353287107	22.447339351006722
52-53	22.405767090475308	27.670311072693394	27.44960445054484	22.474317386286458
54-55	22.42826314123483	27.573620729004183	27.471579757586294	22.526536372174686
56-57	22.441619072683725	27.672061029065915	27.40549102326173	22.480828874988628
58-59	22.34314785205891	27.75754296549713	27.47043059565865	22.428878586785313
60-61	22.47494422608354	27.73218405765502	27.432095101263542	22.3607766149979
62-63	22.398049197186072	27.85272814923373	27.32010896905257	22.42911368452763
64-65	22.32074688275023	27.81771449366119	27.35747057738224	22.504068046206346
66-67	22.39141525318233	27.850004709079606	27.160154678199405	22.598425359538655
68-69	22.396189166378836	27.789938384609954	27.253512908209927	22.560359540801286
70-71	22.327080385377045	27.606202728567155	27.358125433761494	22.708591452294307
72-73	22.430630438938646	27.57793356608665	27.30540012725457	22.686035867720136
74-75	22.35752987992825	27.769856364972217	27.2748931608023	22.597720594297236
76-77	22.457027388671854	27.720879613210847	27.115948888707962	22.706144109409333
78-79	22.396162632957576	27.669725660526762	27.166309066413852	22.767802640101813
80-81	22.391851293950026	27.70856630282026	27.200341504050535	22.69924089917918
82-83	22.431285733550897	27.675341011560374	27.325732843957457	22.567640410931272
84-85	22.553165370302803	27.702939361153316	27.22818131199785	22.515713956546033
86-87	22.392168756097018	27.7641304861766	27.272006974857394	22.571693782868984
88-89	22.482317879563034	27.76048926355621	27.22974667732964	22.52744617955112
90-91	22.46666174183817	27.815618923046415	27.280084750705115	22.437634584410297
92-93	22.50678898466447	27.72328201746277	27.303267524423504	22.46666147344926
94-95	22.303671236812043	27.835956406467062	27.382181217468656	22.47819113925224
96-97	22.279000059816852	27.948243607142683	27.538865861535516	22.233890471504953
98-99	23.4316557603854	29.882679807049488	27.659798828306215	19.025865604258897
100	30.021150221553295	41.400794121234924	28.578055657211777	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1040.0
1	965.0
2	1394.0
3	2024.5
4	2685.0
5	4328.5
6	5809.0
7	6680.0
8	7105.0
9	6871.5
10	6288.0
11	5695.0
12	6088.5
13	8187.5
14	13090.5
15	19865.0
16	25687.0
17	29605.0
18	31435.0
19	31277.0
20	30046.0
21	28778.0
22	28797.0
23	31518.0
24	37662.0
25	47258.5
26	59749.5
27	73543.0
28	87934.5
29	104389.5
30	119957.0
31	135060.5
32	151600.5
33	167839.5
34	184025.5
35	200307.5
36	218273.5
37	234495.5
38	246254.5
39	256185.5
40	267418.0
41	279727.5
42	289010.0
43	296263.0
44	304177.5
45	312402.0
46	318345.5
47	322080.0
48	323141.0
49	318838.0
50	311754.0
51	302813.0
52	290047.0
53	274084.5
54	256816.5
55	239582.5
56	220995.0
57	200227.5
58	178427.5
59	154383.5
60	129775.0
61	107302.5
62	87073.5
63	68771.0
64	52659.5
65	40163.5
66	30576.0
67	23687.5
68	18438.5
69	13756.0
70	10287.0
71	7601.5
72	5531.5
73	3970.0
74	2781.5
75	1893.5
76	1274.0
77	855.5
78	554.0
79	353.0
80	251.5
81	192.0
82	145.0
83	87.0
84	43.0
85	24.0
86	13.5
87	6.5
88	3.5
89	3.0
90	3.0
91	2.5
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.9806738285623304E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001108901995099506
18-19	0.0038100735216239443
20-21	0.0
22-23	0.0013010046243960547
24-25	6.470815236679312E-4
26-27	0.013906796012910913
28-29	0.007603425813203477
30-31	7.704609825004055E-4
32-33	0.007123085849649913
34-35	0.004717407174946195
36-37	0.0021789208159906435
38-39	0.0017972949396065064
40-41	0.004110495733903184
42-43	0.009106136835181161
44-45	0.010299825272707681
46-47	0.009142148484390775
48-49	0.008197549962093105
50-51	0.03542531446134489
52-53	0.013439117710050325
54-55	7.199498914875526E-5
56-57	0.008682861673759559
58-59	0.006871419122496027
60-61	1.989700976130718E-4
62-63	0.005512761497464045
64-65	0.002573786334451727
66-67	0.025329635992560676
68-69	0.03431840146927783
70-71	0.016301687538664542
72-73	0.012080464857721608
74-75	0.01727542021801526
76-77	0.0023458500532645954
78-79	0.0030217512932675847
80-81	0.0016654763872826874
82-83	0.026071177290823453
84-85	9.255596713191466E-4
86-87	0.0102171141706626
88-89	9.751397666822286E-4
90-91	0.0035604833008217552
92-93	0.0013243571810087598
94-95	8.417973033076667E-4
96-97	0.003248662862438554
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	162.0
20-21	556.0
22-23	1218.0
24-25	2305.0
26-27	4427.0
28-29	11029.0
30-31	23245.0
32-33	29183.0
34-35	40678.0
36-37	60605.0
38-39	68334.0
40-41	69909.0
42-43	75160.0
44-45	84667.0
46-47	83571.0
48-49	77130.0
50-51	68317.0
52-53	65570.0
54-55	70675.0
56-57	73827.0
58-59	73514.0
60-61	73132.0
62-63	72045.0
64-65	75425.0
66-67	79425.0
68-69	78230.0
70-71	74441.0
72-73	71593.0
74-75	72565.0
76-77	72974.0
78-79	76506.0
80-81	77898.0
82-83	74867.0
84-85	75769.0
86-87	80490.0
88-89	85186.0
90-91	90650.0
92-93	98713.0
94-95	119956.0
96-97	260618.0
98-99	1429285.0
100-101	2910135.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.22627926389472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22275337749137	76.89349575307581
2	6.6655477303078	11.361596646200644
3	1.6885498490535025	4.317264629593232
4	0.6344821575730077	2.1629821419710247
5	0.2941785998546477	1.2535873752336875
6	0.15760011120512954	0.8059002653753542
7	0.09567685847993422	0.5707927861932105
8	0.05834992753746787	0.3978357775469007
9	0.041262606878979396	0.3164992611321782
>10	0.14009622650845696	1.7972134697933253
>50	0.0011927695007389414	0.06605178586519998
>100	2.82528656977496E-4	0.041750242377055186
>500	2.7256951965030914E-5	0.015029865642351248
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	9.951684571405825E-5	0.0	1.4216692244865464E-5	0.0
5	0.0	9.951684571405825E-5	0.0	1.4216692244865464E-5	0.0
6	0.0	9.951684571405825E-5	0.0	1.4216692244865464E-5	0.0
7	0.0	9.951684571405825E-5	0.0	1.4216692244865464E-5	0.0
8	0.0	9.951684571405825E-5	0.0	1.4216692244865464E-5	0.0
9	0.0	2.2746707591784742E-4	0.0	4.265007673459639E-5	0.0
10-11	0.0	2.2746707591784742E-4	0.0	5.6866768979461854E-5	0.0
12-13	1.4216692244865464E-5	2.2746707591784742E-4	0.0	6.397511510189459E-5	2.1325038367298196E-5
14-15	1.4216692244865464E-5	2.91442191019742E-4	0.0	7.108346122432732E-5	4.975842285702912E-5
16-17	1.4216692244865464E-5	2.985505371421747E-4	0.0	9.240849959162552E-5	5.6866768979461854E-5
18-19	1.4216692244865464E-5	3.4830895999920386E-4	0.0	1.563836146935201E-4	7.108346122432732E-5
20-21	1.4216692244865464E-5	3.98067382856233E-4	0.0	2.0614203755054924E-4	8.530015346919278E-5
22-23	1.4216692244865464E-5	4.407174595908294E-4	0.0	2.701171526524438E-4	8.530015346919278E-5
24-25	1.4216692244865464E-5	4.975842285702912E-4	0.0	2.985505371421748E-4	1.1373353795892371E-4
26-27	1.4216692244865464E-5	5.402343053048876E-4	0.0	4.265007673459639E-4	1.1373353795892371E-4
28-29	1.4216692244865464E-5	5.402343053048876E-4	0.0	0.0014856443395884409	1.2795023020378918E-4
30-31	1.4216692244865464E-5	6.539678432638113E-4	0.0	0.004528016479989651	1.350585763262219E-4
32-33	1.4216692244865464E-5	6.539678432638113E-4	0.0	0.009617592303651487	1.4216692244865465E-4
34-35	1.4216692244865464E-5	7.67701381222735E-4	0.0	0.01575920335343337	1.4216692244865465E-4
36-37	1.4216692244865464E-5	8.885432653040915E-4	0.0	0.02804242545299713	1.4216692244865465E-4
38-39	1.4216692244865464E-5	9.525183804059861E-4	0.0	0.059447098621904934	1.4216692244865465E-4
40-41	1.4216692244865464E-5	0.001165768764078968	0.0	0.09262885832142093	1.4216692244865465E-4
42-43	1.4216692244865464E-5	0.0011942021485686991	0.0	0.11297294492382341	1.4216692244865465E-4
44-45	1.4216692244865464E-5	0.0013861274938743828	0.0	0.13832130719641855	1.563836146935201E-4
46-47	1.4216692244865464E-5	0.0015567278008127685	0.0	0.16349196081595285	1.563836146935201E-4
48-49	1.4216692244865464E-5	0.0015780528391800665	0.0	0.18702058648120518	1.563836146935201E-4
50-51	1.4216692244865464E-5	0.0016633529926492593	0.0	0.20994500272605074	1.8481699918325103E-4
52-53	1.4216692244865464E-5	0.0016775696848941248	0.0	0.2693423429250986	1.8481699918325103E-4
54-55	1.4216692244865464E-5	0.0016775696848941248	0.0	0.3411082053771795	1.8481699918325103E-4
56-57	1.4216692244865464E-5	0.0016775696848941248	0.0	0.4078982255435575	1.990336914281165E-4
58-59	1.4216692244865464E-5	0.0016775696848941248	0.0	0.44907687463081025	2.2035872979541467E-4
60-61	1.4216692244865464E-5	0.0017131114155062885	0.0	0.47655774074013524	2.2746707591784742E-4
62-63	1.4216692244865464E-5	0.0017344364538735865	0.0	0.49596352565437657	2.3457542204028016E-4
64-65	1.4216692244865464E-5	0.0018766033763222413	0.0	0.5037187312739506	2.4168376816271288E-4
66-67	1.4216692244865464E-5	0.0018908200685671067	0.0	0.5113815283939331	2.4168376816271288E-4
68-69	1.4216692244865464E-5	0.001912145106934405	0.0	0.5185396329392229	2.5590046040757837E-4
70-71	1.4216692244865464E-5	0.0019476868375465685	0.0	0.5251361781408406	2.701171526524438E-4
72-73	1.4216692244865464E-5	0.001961903529791434	0.0	0.5301973205800126	2.701171526524438E-4
74-75	1.4216692244865464E-5	0.001990336914281165	0.0	0.534704012021635	2.701171526524438E-4
76-77	1.4216692244865464E-5	0.002075637067750358	0.0	0.5388339611187685	2.701171526524438E-4
78-79	1.4216692244865464E-5	0.0021182871444849543	0.0	0.5414924825685582	2.701171526524438E-4
80-81	1.4216692244865464E-5	0.0021253954906073867	0.0	0.5423810258338624	2.701171526524438E-4
82-83	1.4216692244865464E-5	0.0021325038367298195	0.0	0.5428075266012082	2.701171526524438E-4
84-85	1.4216692244865464E-5	0.002146720528974685	0.0	0.5429710185620242	2.701171526524438E-4
86-87	1.4216692244865464E-5	0.0021609372212195504	0.0	0.5430989687922281	2.701171526524438E-4
88	1.4216692244865464E-5	0.0022604540669336087	0.0	0.5431060771383505	2.701171526524438E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6935	0.0	43.268543	1
GTATCAA	12025	0.0	31.91348	1
TCAACGC	13740	0.0	27.157566	4
ATCAACG	13815	0.0	27.069235	3
CAACGCA	14140	0.0	26.302704	5
TATCAAC	14730	0.0	25.74805	2
AACGCAG	14765	0.0	25.216965	6
ACGCAGA	17165	0.0	21.691143	7
TACATGG	18000	0.0	21.161213	2
GTACATG	18670	0.0	20.926617	1
CGCAGAG	17865	0.0	20.795519	8
ACATGGG	18595	0.0	19.869356	3
CATGGGG	14330	0.0	18.461212	4
GCAGAGT	20910	0.0	17.610996	9
GAGTACT	11465	0.0	17.201006	12-13
GTGGTAT	4415	0.0	16.737064	1
TATACCG	415	1.8189894E-12	16.723694	5
CAGAGTA	20205	0.0	16.20521	10-11
AGAGTAC	18620	0.0	15.896351	10-11
TGGTATC	4300	0.0	15.665594	2
>>END_MODULE