FastQCFastQC Report
Fri 27 May 2016
SRR2140276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8971086
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.042.9284741
GTATCAA157650.031.817181
CTTATAC130950.028.7205961
ATCAACG178450.027.3755283
TCAACGC178850.027.2685854
CAACGCA179900.027.1321545
TATCAAC188850.026.1926542
AACGCAG189300.025.8712436
TATACAC163100.025.4152933
TTATACA167450.023.4123272
ACGCAGA224900.021.6851337
GTACATG236300.021.5904661
TACATGG231950.021.4313892
CGCAGAG230400.021.1674778
ACATGGG234600.020.4225863
TACACAT200200.020.3787525
ACACATC194200.019.7453466
ACATCTC198100.019.6248878
CATGGGG184850.018.2450734
ATACACA251450.018.0460624
Produced by FastQC (version 0.11.5)