FastQCFastQC Report
Fri 27 May 2016
SRR2140275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6271166
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72050.039.037961
GTATCAA121750.030.757681
ATCAACG131900.027.171383
TCAACGC132500.027.0483384
CAACGCA134050.026.6740515
AACGCAG139500.025.7797686
TATCAAC148450.024.5033282
GTACATG176950.022.35141
TACATGG174700.022.1907332
ACGCAGA163000.021.9618457
CGCAGAG166000.021.5400988
ACATGGG178600.021.1981493
CATGGGG134500.018.6430934
GCAGAGT198500.018.0133829
ACCGAAC8150.017.7111648
GAGTACT104000.017.25082812-13
AGAGTAC171850.016.93119610-11
CAGAGTA190800.016.42764910-11
GAGTACA125850.016.090331
ATGGGGG91700.015.6061885
Produced by FastQC (version 0.11.5)