FastQCFastQC Report
Fri 27 May 2016
SRR2140274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5729182
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.044.8638841
GTATCAA125900.034.6862071
TCAACGC147800.028.7676354
ATCAACG149200.028.580383
CAACGCA151450.028.1286265
TATCAAC154400.027.8308872
AACGCAG157050.027.282736
ACGCAGA184350.023.1309597
AATATCG5654.5474735E-1122.6829694
GTACATG191300.022.6560231
CGCAGAG189100.022.4846978
TACATGG190750.022.160862
ACATGGG194450.021.0413283
GTGGTAT40500.020.2048721
GGACCGA10300.019.9613916
ACCGAAC8550.019.7185868
GCAGAGT220550.019.2038469
CATGGGG147950.018.871784
GAGTACT117950.018.16390412-13
TGGTATC41500.017.637212
Produced by FastQC (version 0.11.5)