FastQCFastQC Report
Fri 27 May 2016
SRR2140273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7696427
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65250.035.9568331
GTATCAA103450.029.8973561
TCAACGC117250.024.8452154
ATCAACG118450.024.697973
CAACGCA118750.024.5652585
GTACATG297750.024.2009831
TACATGG296000.023.8626792
AACGCAG126650.023.3874666
TATCAAC129750.023.0520952
ACATGGG303550.022.702653
CATGGGG213550.020.767654
ACGCAGA154800.018.9246467
CGCAGAG158300.018.4287828
CGTCGAA4301.7743097E-518.30309794
ATGGGGG134950.017.1419055
GTACTAG20200.016.1596781
GAGTACA214750.016.055271
AGTACAT215400.015.1550762
GCAGAGT192950.015.076859
TGCGCGA9252.059096E-914.88981694
Produced by FastQC (version 0.11.5)