FastQCFastQC Report
Fri 27 May 2016
SRR2140272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9626106
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142050.048.9323431
GTATCAA256500.037.696831
TCAACGC311500.030.51574
ATCAACG316750.029.9691773
CAACGCA319250.029.721025
TATCAAC322500.029.6220252
AACGCAG325000.029.2774856
ACGCAGA376100.025.2790977
CGCAGAG384350.024.7479368
GTGGTAT71000.021.9909571
GTACATG315700.021.8537351
GCAGAGT438650.021.6059559
TACATGG320750.021.0671332
TGGTATC69450.020.9953612
ACATGGG316300.020.656073
GAGTACT268600.019.87565412-13
CAGAGTA431950.018.93327310-11
AGAGTAC401600.018.73048610-11
CATGGGG219850.018.1164744
GTACTTT297200.017.93175914-15
Produced by FastQC (version 0.11.5)