FastQCFastQC Report
Fri 27 May 2016
SRR2140271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8514190
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121850.050.8259121
GTATCAA232150.040.0159651
TCAACGC283850.032.1895524
ATCAACG285000.032.0749663
CAACGCA290250.031.4346965
TATCAAC293650.031.2788472
AACGCAG297700.030.7084436
GTGGTAT63150.026.8657281
ACGCAGA342050.026.7423827
TGGTATC60000.026.6770112
CGCAGAG346650.026.3878238
GCAGAGT394350.023.1738669
GTACATG254900.021.4731751
TACATGG254200.020.9146482
GAGTACT259750.020.49584412-13
ACATGGG255450.020.1634033
AGAGTAC365400.019.63186810-11
CAGAGTA390550.019.41196310-11
GTACTTT288350.018.43054614-15
AGTACTT284200.017.85771412-13
Produced by FastQC (version 0.11.5)