##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8514190
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.631918479620495	33.0	33.0	33.0	33.0	33.0
2	32.662956781561135	33.0	33.0	33.0	33.0	33.0
3	32.699937633527085	33.0	33.0	33.0	33.0	33.0
4	36.62918351598919	37.0	37.0	37.0	37.0	37.0
5	36.641039723097556	37.0	37.0	37.0	37.0	37.0
6	36.62288790830367	37.0	37.0	37.0	37.0	37.0
7	36.60332127894726	37.0	37.0	37.0	37.0	37.0
8	36.585675912799694	37.0	37.0	37.0	37.0	37.0
9	36.61622268236908	37.0	37.0	37.0	37.0	37.0
10-11	36.59487537863261	37.0	37.0	37.0	37.0	37.0
12-13	36.59080352916719	37.0	37.0	37.0	37.0	37.0
14-15	38.706892963394054	40.0	37.0	40.0	37.0	40.0
16-17	38.64789052158808	40.0	37.0	40.0	37.0	40.0
18-19	38.67531838025696	40.0	37.0	40.0	37.0	40.0
20-21	38.68636226185349	40.0	37.0	40.0	37.0	40.0
22-23	38.67807291639176	40.0	37.0	40.0	37.0	40.0
24-25	38.680617774439135	40.0	37.0	40.0	37.0	40.0
26-27	38.614163480259535	40.0	37.0	40.0	37.0	40.0
28-29	38.512638585550036	40.0	37.0	40.0	37.0	40.0
30-31	38.38354930911107	40.0	37.0	40.0	37.0	40.0
32-33	38.27311025227688	40.0	37.0	40.0	37.0	40.0
34-35	38.23249676702058	40.0	37.0	40.0	37.0	40.0
36-37	38.21323197008617	40.0	37.0	40.0	37.0	40.0
38-39	38.174944404864995	40.0	37.0	40.0	37.0	40.0
40-41	38.14504207637741	40.0	37.0	40.0	37.0	40.0
42-43	38.10564998580894	40.0	37.0	40.0	37.0	40.0
44-45	38.00896627597165	40.0	37.0	40.0	33.0	40.0
46-47	37.92357638488765	40.0	37.0	40.0	33.0	40.0
48-49	37.860136430726385	40.0	37.0	40.0	33.0	40.0
50-51	37.831332221284384	40.0	37.0	40.0	33.0	40.0
52-53	37.783101618294246	40.0	37.0	40.0	33.0	40.0
54-55	37.680611897630314	40.0	37.0	40.0	33.0	40.0
56-57	37.51526857657362	40.0	37.0	40.0	33.0	40.0
58-59	37.297921938965004	38.5	37.0	40.0	33.0	40.0
60-61	37.15038143846682	37.0	37.0	40.0	33.0	40.0
62-63	37.02985998409454	37.0	37.0	40.0	33.0	40.0
64-65	36.858798746303776	37.0	37.0	40.0	33.0	40.0
66-67	36.64075146890876	37.0	37.0	40.0	33.0	40.0
68-69	36.435908214599905	37.0	37.0	40.0	33.0	40.0
70-71	36.209567102428565	37.0	37.0	40.0	33.0	40.0
72-73	35.908157725428964	37.0	37.0	37.0	33.0	40.0
74-75	35.56948818904664	37.0	33.0	37.0	33.0	40.0
76-77	33.32540915896857	35.0	33.0	37.0	27.0	37.0
78-79	35.06298863358009	37.0	33.0	37.0	33.0	37.0
80-81	35.28866171430235	37.0	33.0	37.0	33.0	37.0
82-83	35.1394310301321	37.0	33.0	37.0	33.0	37.0
84-85	34.96555464272218	37.0	33.0	37.0	33.0	37.0
86-87	34.84455191462244	37.0	33.0	37.0	33.0	37.0
88-89	34.73303662370313	37.0	33.0	37.0	33.0	37.0
90-91	34.661160412635326	37.0	33.0	37.0	33.0	37.0
92-93	34.58070644635144	37.0	33.0	37.0	33.0	37.0
94-95	34.575330077106514	37.0	33.0	37.0	33.0	37.0
96-97	34.613608679520354	37.0	33.0	37.0	33.0	37.0
98-99	34.622021258309985	37.0	33.0	37.0	33.0	37.0
100	34.550595544662634	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	42.0
21	214.0
22	821.0
23	2518.0
24	6108.0
25	12413.0
26	22654.0
27	36539.0
28	55340.0
29	79279.0
30	109802.0
31	148354.0
32	199737.0
33	270632.0
34	381141.0
35	568269.0
36	1021028.0
37	2521725.0
38	3000865.0
39	76705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50845823266805	20.49264815560846	14.853380063165138	23.14551354855835
2	14.184461053386975	20.737924080545866	42.90075762069166	22.176857245375494
3	18.217810502232158	26.179401681193397	30.42810883947856	25.174678977095883
4	12.310260870382269	18.00198257262288	39.36861874118384	30.319137815811015
5	13.148579019260787	37.680119893965255	35.728190233011006	13.443110853762954
6	29.355640407366995	38.106972007906805	19.014762414275463	13.522625170450741
7	26.164379700241597	32.86131740071575	23.350171889516208	17.62413100952645
8	23.690486117880855	37.36583280382514	21.28573593025291	17.657945148041094
9	24.971183400887227	17.56157661503913	22.048298193956207	35.418941790117444
10-11	23.723812834808715	27.27459100630829	28.972926373501178	20.028669785381815
12-13	24.509911996144968	24.744461694728315	29.497280657681184	21.248345651445536
14-15	21.62556989671021	26.165041088939855	27.358548017550778	24.850840996799153
16-17	20.865132208701002	29.427021243359615	28.134602352073422	21.57324419586596
18-19	20.823031903210993	28.339295928326713	30.26997283358722	20.567699334875073
20-21	21.545470113458286	27.442756994801247	30.400696925498643	20.61107596624182
22-23	21.30024087888461	27.33146920849206	30.268273402966773	21.100016509656562
24-25	21.021747471892223	27.51855621825106	30.350821727546386	21.10887458231033
26-27	20.992442463814264	27.662009132833287	30.27995560328696	21.065592800065485
28-29	20.92746049231304	27.666268435813407	30.31388633454425	21.092384737329304
30-31	21.132815288121876	27.645876816695164	30.18202860250244	21.039279292680522
32-33	20.9462568791421	27.798164131898474	30.068457836846928	21.187121152112496
34-35	21.121681203094013	27.799701497772983	30.06971449649201	21.00890280264099
36-37	21.101728064442867	27.721512727815416	29.997480109595987	21.179279098145734
38-39	21.02749212647206	27.93446618277239	29.99765988024009	21.04038181051546
40-41	21.22892556686909	27.819809188415253	29.825871688810583	21.125393555905077
42-43	21.133149856988634	27.945467703992055	29.559033774237918	21.362348664781393
44-45	21.234222733885204	28.046401509913498	29.384121762748972	21.335253993452326
46-47	21.336035942246614	28.123413037430407	29.036505364073374	21.50404565624961
48-49	21.327986950127322	28.23156828140255	28.869921469087057	21.570523299383073
50-51	21.3668141403197	28.344570270713476	28.758805862825465	21.52980972614136
52-53	21.52053806951959	28.30492039808729	28.56012897879763	21.614412553595493
54-55	21.525162450701803	28.208314937563028	28.60426846071108	21.662254151024086
56-57	21.459765585260662	28.28498712699587	28.6378363595657	21.61741092817777
58-59	21.46491553860953	28.276289010259966	28.61473735075925	21.644058100371254
60-61	21.515828389620317	28.27328904927065	28.602208651007576	21.608673910101455
62-63	21.53407589775612	28.353931155303936	28.540208518834042	21.571784428105904
64-65	21.53914824391629	28.27322608864326	28.468275176007506	21.71935049143294
66-67	21.572084437105637	28.296957823023106	28.343968129232337	21.78698961063892
68-69	21.494910470274515	28.308925985168855	28.40992146206331	21.786242082493324
70-71	21.57638661777167	28.173655118594233	28.41762122090173	21.832337042732362
72-73	21.652470026075033	28.19275138812133	28.27996846691275	21.874810118890892
74-75	21.5429147169534	28.32670644721768	28.253065535900955	21.877313299927962
76-77	21.69452046334547	28.249813873479734	28.163098686900568	21.89256697627423
78-79	21.650483930066823	28.1683467966414	28.13800796124511	22.043161312046664
80-81	21.668979832553124	28.282423253514356	28.127963247221814	21.920633666710707
82-83	21.77964749980062	28.237987135310245	28.116174088567806	21.866191276321327
84-85	21.79262583096279	28.24115338430787	28.08626281583844	21.879957968890896
86-87	21.750452350781117	28.252423005575245	28.142485387818166	21.854639255825475
88-89	21.837335742159354	28.23139311227731	28.02840423140079	21.90286691416255
90-91	21.835445657603138	28.29888510827046	28.034333796350918	21.831335437775483
92-93	21.848521710300513	28.247658178048514	28.043354350582188	21.860465761068788
94-95	21.771555845151205	28.264098111757267	28.065602096809318	21.89874394628221
96-97	21.927795854839637	28.35335952556119	28.057593526632107	21.661251092967067
98-99	22.987463968819696	30.237652552768772	28.307836020637584	18.46704745777395
100	21.99089051044849	31.097312143558014	22.121936902002286	24.78986044399121
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2806.0
1	2376.5
2	2771.0
3	3819.0
4	5088.0
5	8373.0
6	11242.5
7	13393.5
8	15111.0
9	15125.5
10	13870.0
11	12329.5
12	13122.0
13	17456.5
14	27778.5
15	42365.5
16	54895.0
17	62625.5
18	64127.5
19	59944.5
20	53049.0
21	45738.5
22	40539.0
23	40730.0
24	46336.0
25	56872.5
26	71464.0
27	88290.0
28	106503.5
29	126721.0
30	146852.5
31	165969.5
32	186944.5
33	208088.0
34	227997.0
35	246579.0
36	267705.0
37	287713.5
38	303295.5
39	317152.0
40	330187.0
41	340388.0
42	345219.5
43	351644.5
44	358450.0
45	363705.5
46	364669.5
47	360114.5
48	354669.5
49	343821.5
50	328155.5
51	309451.5
52	287095.0
53	261069.5
54	231709.0
55	203384.0
56	176982.0
57	151426.5
58	129425.0
59	109367.5
60	89848.5
61	72744.5
62	56641.0
63	42934.0
64	32094.5
65	23673.0
66	17925.5
67	13760.0
68	10513.0
69	7717.0
70	5578.5
71	4166.0
72	3049.0
73	2196.5
74	1532.0
75	1036.5
76	714.0
77	472.0
78	312.5
79	222.5
80	161.5
81	119.5
82	89.0
83	60.0
84	37.0
85	27.0
86	19.5
87	12.0
88	7.0
89	4.0
90	2.5
91	1.0
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.1107844668723624E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.00754622577132998
14-15	4.404411928791817E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.2868364293341176E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0029526907486943053
36-37	0.032366115169850766
38-39	0.1404949001728638
40-41	0.19100459483083318
42-43	0.05947562285971642
44-45	0.007605024029170588
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.8813678422184588E-5
62-63	0.012763490687888426
64-65	0.07615912124872506
66-67	0.2056401239564886
68-69	0.2225960546714775
70-71	0.08738238396951067
72-73	0.005766792363451793
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	123.0
20-21	611.0
22-23	1218.0
24-25	2291.0
26-27	3549.0
28-29	6087.0
30-31	12957.0
32-33	15740.0
34-35	20605.0
36-37	23129.0
38-39	25241.0
40-41	33499.0
42-43	39937.0
44-45	50931.0
46-47	52171.0
48-49	42506.0
50-51	37496.0
52-53	35274.0
54-55	44259.0
56-57	41837.0
58-59	41341.0
60-61	46611.0
62-63	49969.0
64-65	59335.0
66-67	70635.0
68-69	68625.0
70-71	68349.0
72-73	64783.0
74-75	67208.0
76-77	61425.0
78-79	67348.0
80-81	68949.0
82-83	72424.0
84-85	76947.0
86-87	82587.0
88-89	88834.0
90-91	93585.0
92-93	92951.0
94-95	109923.0
96-97	292974.0
98-99	545351.0
100-101	5834575.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.19752228297092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3728518420575	76.14345536306352
2	7.568040110697553	12.89556531939145
3	1.7777646611450661	4.543834329953551
4	0.616278997661886	2.1002177454330204
5	0.2708484987266537	1.153781050278665
6	0.1412907435662933	0.7222572764020082
7	0.07732112275247144	0.46112976550536106
8	0.04666337683045268	0.31804832698489294
9	0.030001372143544272	0.23004383145473847
>10	0.09661610089684994	1.2430263226576965
>50	0.001783621704664765	0.10248291848331738
>100	5.176935466778084E-4	0.07401574687619396
>500	2.1858270380798703E-5	0.012142003515486163
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.8725492383890894E-5	0.0	0.0	0.0
3	0.0	7.047059086066908E-5	0.0	0.0	0.0
4	0.0	1.057058862910036E-4	0.0	0.0	0.0
5	0.0	1.057058862910036E-4	0.0	0.0	0.0
6	0.0	1.057058862910036E-4	0.0	0.0	1.1745098476778178E-5
7	0.0	1.1745098476778179E-4	0.0	0.0	1.1745098476778178E-5
8	0.0	1.2919608324455997E-4	0.0	0.0	2.3490196953556356E-5
9	1.1745098476778178E-5	2.3490196953556357E-4	0.0	1.1745098476778178E-5	3.523529543033454E-5
10-11	1.7617647715167265E-5	2.3490196953556357E-4	0.0	1.1745098476778178E-5	3.523529543033454E-5
12-13	3.523529543033454E-5	2.3490196953556357E-4	0.0	2.3490196953556356E-5	4.110784466872362E-5
14-15	3.523529543033454E-5	3.5822550354173445E-4	0.0	2.3490196953556356E-5	9.983333705261452E-5
16-17	4.110784466872362E-5	3.640980527801235E-4	0.0	2.3490196953556356E-5	1.1745098476778179E-4
18-19	5.28529431455018E-5	3.758431512569017E-4	0.0	3.523529543033454E-5	1.2332353400617088E-4
20-21	5.8725492383890894E-5	4.1107844668723624E-4	0.0	3.523529543033454E-5	1.2919608324455997E-4
22-23	5.8725492383890894E-5	4.6393138983273803E-4	0.0	3.523529543033454E-5	1.4094118172133815E-4
24-25	5.8725492383890894E-5	5.402745299317962E-4	0.0	4.698039390711271E-5	1.5268628019811633E-4
26-27	5.8725492383890894E-5	5.637647268853526E-4	0.0	7.047059086066908E-5	1.644313786748945E-4
28-29	5.8725492383890894E-5	5.696372761237417E-4	0.0	3.112451096346217E-4	1.644313786748945E-4
30-31	5.8725492383890894E-5	6.459804162227998E-4	0.0	0.001250852987776876	1.644313786748945E-4
32-33	5.8725492383890894E-5	6.459804162227998E-4	0.0	0.00303023540700877	2.114117725820072E-4
34-35	7.634314009905815E-5	7.399412040370253E-4	0.0	0.004803745277002275	2.114117725820072E-4
36-37	9.396078781422542E-5	8.339019918512507E-4	0.0	0.007499245377422867	2.172843218203963E-4
38-39	1.057058862910036E-4	8.51519639566418E-4	0.0	0.01380636325945275	2.231568710587854E-4
40-41	1.5855882943650542E-4	9.86588272049367E-4	0.0	0.020142843887674577	2.231568710587854E-4
42-43	1.644313786748945E-4	0.0010100784690029235	0.0	0.024611853858088673	2.231568710587854E-4
44-45	1.644313786748945E-4	0.001104039256817149	0.0	0.02965050110462651	2.231568710587854E-4
46-47	1.703039279132836E-4	0.001174509847677818	0.0	0.035094354248613194	2.231568710587854E-4
48-49	1.9966667410522903E-4	0.001186254946154596	0.0	0.04156590350931798	2.231568710587854E-4
50-51	2.3490196953556357E-4	0.0013448137755911016	0.0	0.047978727277638855	2.231568710587854E-4
52-53	2.3490196953556357E-4	0.0013506863248294906	0.0	0.06674739464353038	2.4077451877395267E-4
54-55	2.3490196953556357E-4	0.0013565588740678796	0.0	0.09149431713410201	2.64264715727509E-4
56-57	2.3490196953556357E-4	0.0013976667187366033	0.0	0.11943003386111892	2.8775491268106536E-4
58-59	2.3490196953556357E-4	0.0014035392679749923	0.0	0.13426409323728974	3.0537256039623266E-4
60-61	2.3490196953556357E-4	0.0014446471126437158	0.0	0.14543955443794418	3.0537256039623266E-4
62-63	2.5839216648911994E-4	0.0014798824080740505	0.0	0.15774254509236932	3.0537256039623266E-4
64-65	2.8775491268106536E-4	0.0016208235897953887	0.0	0.1621175942749692	3.0537256039623266E-4
66-67	3.0537256039623266E-4	0.001638441237510556	0.0	0.16773762389610755	3.0537256039623266E-4
68-69	3.0537256039623266E-4	0.0016795490821792797	0.0	0.1741152123689981	3.112451096346217E-4
70-71	3.0537256039623266E-4	0.0016912941806560577	0.0	0.17911275177086722	3.347353065881781E-4
72-73	3.0537256039623266E-4	0.0016912941806560577	0.0	0.18256581072304	3.640980527801235E-4
74-75	3.0537256039623266E-4	0.0017324020253247814	0.0	0.18536114416051322	3.640980527801235E-4
76-77	3.1711765887301084E-4	0.0018028726161854504	0.0	0.18809775210560253	3.640980527801235E-4
78-79	3.1711765887301084E-4	0.0018322353623773959	0.0	0.18959525216139173	3.640980527801235E-4
80-81	3.1711765887301084E-4	0.0018381079116157849	0.0	0.1901531443390387	3.699706020185126E-4
82-83	3.1711765887301084E-4	0.001843980460854174	0.0	0.19052311494105723	3.758431512569017E-4
84-85	3.1711765887301084E-4	0.0018557255593309521	0.0	0.19072865416440082	3.758431512569017E-4
86-87	3.1711765887301084E-4	0.0018792157562845086	0.0	0.1908637227968838	3.758431512569017E-4
88	3.1711765887301084E-4	0.001973176544098734	0.0	0.1908695953461222	3.758431512569017E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12185	0.0	50.825912	1
GTATCAA	23215	0.0	40.015965	1
TCAACGC	28385	0.0	32.189552	4
ATCAACG	28500	0.0	32.074966	3
CAACGCA	29025	0.0	31.434696	5
TATCAAC	29365	0.0	31.278847	2
AACGCAG	29770	0.0	30.708443	6
GTGGTAT	6315	0.0	26.865728	1
ACGCAGA	34205	0.0	26.742382	7
TGGTATC	6000	0.0	26.677011	2
CGCAGAG	34665	0.0	26.387823	8
GCAGAGT	39435	0.0	23.173866	9
GTACATG	25490	0.0	21.473175	1
TACATGG	25420	0.0	20.914648	2
GAGTACT	25975	0.0	20.495844	12-13
ACATGGG	25545	0.0	20.163403	3
AGAGTAC	36540	0.0	19.631868	10-11
CAGAGTA	39055	0.0	19.411963	10-11
GTACTTT	28835	0.0	18.430546	14-15
AGTACTT	28420	0.0	17.857714	12-13
>>END_MODULE