FastQCFastQC Report
Fri 27 May 2016
SRR2140270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7785713
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132400.048.9155461
GTATCAA235900.036.7623941
TCAACGC282750.030.0512314
ATCAACG285050.029.8237423
CAACGCA288800.029.3921095
TATCAAC292050.029.3433282
GTGGTAT63200.029.1337171
AACGCAG294050.028.898446
TGGTATC62150.027.5637872
ACGCAGA335900.025.2488737
CGCAGAG341050.024.8427078
GCAGAGT381300.022.1194659
GAGTACT265800.020.52649712-13
GTACATG226250.019.5239471
CAGAGTA378950.019.51119810-11
TACATGG221300.019.3429162
AGAGTAC355250.018.72616210-11
ACATGGG219450.018.649193
GTACTTT293800.018.50369314-15
AGTACTT285050.017.32669412-13
Produced by FastQC (version 0.11.5)