FastQCFastQC Report
Fri 27 May 2016
SRR2140269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6833332
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119900.050.8221471
GTATCAA226000.039.4026451
TCAACGC278150.031.5493074
ATCAACG279000.031.4531883
CAACGCA283250.030.9964965
TATCAAC285750.030.9368972
AACGCAG286000.030.7602066
ACGCAGA325450.026.9810357
CGCAGAG329100.026.6557438
GTGGTAT56500.026.5996511
TGGTATC55150.025.8412552
GCAGAGT367350.023.6803519
GAGTACT259800.021.22173312-13
CAGAGTA367550.019.9951310-11
GTACATG208850.019.8716561
AGAGTAC347050.019.54585310-11
GTACTTT281650.019.49692214-15
TACATGG209250.019.4621752
ACATGGG202450.019.2839413
AGTACTT274350.018.86040712-13
Produced by FastQC (version 0.11.5)