##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6833332
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63692968525457	33.0	33.0	33.0	33.0	33.0
2	32.67367647291248	33.0	33.0	33.0	33.0	33.0
3	32.707640430759106	33.0	33.0	33.0	33.0	33.0
4	36.643204808430205	37.0	37.0	37.0	37.0	37.0
5	36.657017103808215	37.0	37.0	37.0	37.0	37.0
6	36.63670022179517	37.0	37.0	37.0	37.0	37.0
7	36.61783563274842	37.0	37.0	37.0	37.0	37.0
8	36.60122426365351	37.0	37.0	37.0	37.0	37.0
9	36.629205049600984	37.0	37.0	37.0	37.0	37.0
10-11	36.60965916773837	37.0	37.0	37.0	37.0	37.0
12-13	36.604239630095535	37.0	37.0	37.0	37.0	37.0
14-15	38.72016975027702	40.0	37.0	40.0	37.0	40.0
16-17	38.66516020295809	40.0	37.0	40.0	37.0	40.0
18-19	38.704166137398275	40.0	37.0	40.0	37.0	40.0
20-21	38.7213488557167	40.0	37.0	40.0	37.0	40.0
22-23	38.71241613043595	40.0	37.0	40.0	37.0	40.0
24-25	38.71490792664855	40.0	37.0	40.0	37.0	40.0
26-27	38.64577051754039	40.0	37.0	40.0	37.0	40.0
28-29	38.543667088043605	40.0	37.0	40.0	37.0	40.0
30-31	38.405954968401744	40.0	37.0	40.0	37.0	40.0
32-33	38.292417036259295	40.0	37.0	40.0	37.0	40.0
34-35	38.2501796472947	40.0	37.0	40.0	37.0	40.0
36-37	38.22843187949874	40.0	37.0	40.0	37.0	40.0
38-39	38.189325142192054	40.0	37.0	40.0	37.0	40.0
40-41	38.158199352263324	40.0	37.0	40.0	37.0	40.0
42-43	38.11992615169497	40.0	37.0	40.0	37.0	40.0
44-45	38.022520011362204	40.0	37.0	40.0	33.0	40.0
46-47	37.94140237326967	40.0	37.0	40.0	33.0	40.0
48-49	37.87828202409154	40.0	37.0	40.0	33.0	40.0
50-51	37.854443269513396	40.0	37.0	40.0	33.0	40.0
52-53	37.81631677978173	40.0	37.0	40.0	33.0	40.0
54-55	37.72304293429599	40.0	37.0	40.0	33.0	40.0
56-57	37.56684450533626	40.0	37.0	40.0	33.0	40.0
58-59	37.361216277611724	40.0	37.0	40.0	33.0	40.0
60-61	37.21961956673344	37.0	37.0	40.0	33.0	40.0
62-63	37.104295989348955	37.0	37.0	40.0	33.0	40.0
64-65	36.93972253760714	37.0	37.0	40.0	33.0	40.0
66-67	36.7275080547542	37.0	37.0	40.0	33.0	40.0
68-69	36.52630357718003	37.0	37.0	40.0	33.0	40.0
70-71	36.30182042574833	37.0	37.0	40.0	33.0	40.0
72-73	36.00385590286723	37.0	37.0	37.0	33.0	40.0
74-75	35.663047888709656	37.0	35.0	37.0	33.0	40.0
76-77	33.42550442736865	35.0	33.0	37.0	27.0	37.0
78-79	35.153136684982954	37.0	33.0	37.0	33.0	37.0
80-81	35.37080022580202	37.0	33.0	37.0	33.0	37.0
82-83	35.216025647008586	37.0	33.0	37.0	33.0	37.0
84-85	35.038193914429606	37.0	33.0	37.0	33.0	37.0
86-87	34.913886920721325	37.0	33.0	37.0	33.0	37.0
88-89	34.801897063513614	37.0	33.0	37.0	33.0	37.0
90-91	34.726458071587416	37.0	33.0	37.0	33.0	37.0
92-93	34.644383150377735	37.0	33.0	37.0	33.0	37.0
94-95	34.63587913066061	37.0	33.0	37.0	33.0	37.0
96-97	34.66699602124533	37.0	33.0	37.0	33.0	37.0
98-99	34.675351461290326	37.0	33.0	37.0	33.0	37.0
100	34.60279173149972	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	9.0
20	41.0
21	168.0
22	619.0
23	1735.0
24	4443.0
25	9316.0
26	16759.0
27	27405.0
28	41938.0
29	60359.0
30	83807.0
31	115075.0
32	155152.0
33	212905.0
34	300041.0
35	448633.0
36	804913.0
37	2010706.0
38	2476280.0
39	63022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31340922896901	20.657714370926858	15.33130767410506	22.697568725999076
2	14.040221497309282	20.87370236771018	43.20391681280129	21.88215932217925
3	17.928942707443852	26.491165143324686	30.83595687081454	24.743935278416924
4	12.037423622534895	18.369020906494942	39.98379414080775	29.609761330162403
5	12.872726346784608	37.762900699527066	35.97236838080872	13.392004572879609
6	28.654897589477223	38.663866860832584	19.19503094464471	13.486204605045474
7	25.645922077253086	33.30394308369621	23.535868006998637	17.514266832052066
8	23.52098507726538	37.349056068108496	21.35773587468017	17.772222979945948
9	24.85171216618774	17.742061998451124	22.20480433264475	35.20142150271639
10-11	23.46653872517829	27.468846823189626	29.12824226892532	19.93637218270677
12-13	24.428843278162134	24.957336444785447	29.65889279283927	20.954927484213147
14-15	21.49855223726392	26.384447811614354	27.832678842527375	24.28432110859435
16-17	20.45423082033772	29.628305781132834	28.479532386250224	21.437931012279222
18-19	20.392935686426476	28.339520163808814	30.986501168097792	20.28104298166692
20-21	21.148683534255973	27.402656815076632	31.22416269875185	20.224496951915548
22-23	20.843200162635743	27.281860165315603	31.146244613843667	20.728695058204995
24-25	20.612375199886227	27.457997633900437	31.185764580047337	20.743862586165996
26-27	20.589446856691996	27.62289148810576	31.080646713065192	20.707014942137057
28-29	20.52645656354965	27.74988826081819	31.037280264285418	20.686374911346743
30-31	20.774166369759243	27.613739990650533	30.96794558592657	20.644148053663653
32-33	20.587810170827677	27.772594337851412	30.846516690403043	20.793078800917865
34-35	20.670165293816098	27.889547867965607	30.80479547793564	20.63549136028265
36-37	20.683448544172755	27.82581093890532	30.727951692391827	20.762788824530094
38-39	20.600793862712834	27.985703754852054	30.72723150000171	20.686270882433398
40-41	20.885472178542923	27.911685035870132	30.466924082455897	20.73591870313105
42-43	20.798025685387728	28.07863344799698	30.15453894806312	20.968801918552167
44-45	20.947965042819884	28.227511852094565	29.86532864851663	20.95919445656892
46-47	21.03081020385011	28.25658813641404	29.47459131779806	21.23801034193779
48-49	21.03884138289218	28.43640811589494	29.2576480347163	21.267102466496578
50-51	21.11005019914623	28.572808200755695	29.057413235725647	21.259728364372428
52-53	21.239268729878017	28.503506526236254	28.941080041563676	21.316144702322052
54-55	21.21435048508861	28.418595918231617	28.98846582247189	21.378587774207883
56-57	21.222703233633933	28.528711078791186	28.896713214936355	21.35187247263853
58-59	21.19070895755229	28.55025383708983	28.889793594315087	21.36924361104279
60-61	21.262281600300128	28.500406876817387	28.93590807348101	21.301403449401477
62-63	21.251911702918978	28.60407153666418	28.84430696256239	21.29970979785446
64-65	21.275311862859574	28.483354700745974	28.812980660775224	21.428352775619228
66-67	21.340241104556952	28.498849284073195	28.667302169888874	21.49360744148097
68-69	21.26323961625908	28.520321796870345	28.693666549597342	21.52277203727323
70-71	21.360082623921816	28.270494383234794	28.755046086236153	21.614376906607234
72-73	21.442909800668637	28.30671484893345	28.668457075140292	21.581918275257625
74-75	21.272608982248915	28.449745809597253	28.6346940959547	21.64295111219913
76-77	21.4580538683464	28.343028516766207	28.541594350134503	21.65732326475289
78-79	21.39268048966266	28.275265459525723	28.557810473528217	21.7742435772834
80-81	21.419450168129632	28.348911940355	28.528806031750165	21.702831859765208
82-83	21.548205356818865	28.240369662392684	28.599669868461664	21.61175511232678
84-85	21.625745115758463	28.207382505359597	28.541048216232245	21.625824162649693
86-87	21.467568772201957	28.26905474834428	28.56212926827774	21.701247211176014
88-89	21.612929704556425	28.164670813022603	28.51667160907696	21.705727873344017
90-91	21.69666598718963	28.138854179770707	28.508236817122167	21.6562430159175
92-93	21.69870501522942	28.07869269011707	28.518717266604938	21.70388502804857
94-95	21.58827577909515	28.138967124923496	28.525721741430754	21.747035354550597
96-97	21.710134242269127	28.205646913900097	28.541504625045917	21.542714218784866
98-99	22.811377197468424	30.082807361696645	28.804687027504944	18.30112841332998
100	21.83421339534306	31.00435315873547	22.615746411037545	24.545687034883922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1591.0
1	1426.5
2	2143.0
3	3263.5
4	4553.0
5	7788.0
6	10743.5
7	12923.5
8	14820.5
9	14991.5
10	13419.5
11	11672.5
12	12392.0
13	16763.5
14	27315.0
15	42098.5
16	55046.0
17	63224.0
18	64590.5
19	60312.5
20	53402.5
21	46112.0
22	40806.0
23	39912.5
24	44415.0
25	53200.0
26	65051.5
27	78896.0
28	93585.0
29	110050.0
30	126041.0
31	141311.5
32	158642.0
33	175762.5
34	192932.0
35	208995.0
36	225746.0
37	241749.5
38	253410.5
39	262819.0
40	272968.0
41	280650.0
42	283437.5
43	287607.5
44	291503.5
45	294635.5
46	293821.0
47	287687.5
48	281428.5
49	272561.0
50	260004.5
51	243229.5
52	221693.0
53	196937.5
54	172964.5
55	150984.0
56	129803.5
57	110319.0
58	93137.5
59	78560.5
60	64361.0
61	51344.0
62	40113.0
63	30205.0
64	22170.5
65	16325.0
66	12468.0
67	9574.0
68	7374.0
69	5483.0
70	4010.0
71	3051.5
72	2178.5
73	1522.0
74	1101.0
75	746.0
76	518.0
77	349.5
78	230.5
79	165.0
80	126.5
81	95.0
82	71.0
83	49.0
84	33.0
85	20.0
86	11.5
87	10.5
88	10.5
89	9.0
90	6.5
91	5.0
92	4.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.463414919690716E-5
2	3.073171331350504E-4
3	1.463414919690716E-5
4	1.463414919690716E-5
5	1.463414919690716E-5
6	1.463414919690716E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.007456099015824199
14-15	4.390244759072148E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.3908118164649446E-5
24-25	5.1236430214444956E-5
26-27	0.0
28-29	7.325957949587447E-6
30-31	1.4668129176933135E-5
32-33	1.4696685037767908E-5
34-35	0.002968595792313797
36-37	0.03212348898778421
38-39	0.1398141929100516
40-41	0.19015299523205023
42-43	0.05950853121832095
44-45	0.007140671762663112
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.01280127877868412
64-65	0.07773612893931586
66-67	0.20382235197596793
68-69	0.22100564330422004
70-71	0.08884497481926575
72-73	0.005786473459597408
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	100.0
20-21	545.0
22-23	1112.0
24-25	2068.0
26-27	3220.0
28-29	5905.0
30-31	12436.0
32-33	15627.0
34-35	20223.0
36-37	25712.0
38-39	29773.0
40-41	37580.0
42-43	47479.0
44-45	58479.0
46-47	59963.0
48-49	47271.0
50-51	39841.0
52-53	40615.0
54-55	39516.0
56-57	38201.0
58-59	39284.0
60-61	36444.0
62-63	26268.0
64-65	41631.0
66-67	51906.0
68-69	50548.0
70-71	52467.0
72-73	49434.0
74-75	49909.0
76-77	45796.0
78-79	51105.0
80-81	54226.0
82-83	51343.0
84-85	59174.0
86-87	64009.0
88-89	68758.0
90-91	73289.0
92-93	71311.0
94-95	79375.0
96-97	223228.0
98-99	430609.0
100-101	4637552.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.76106549500236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71865949801595	74.31169448842128
2	7.817717453132446	13.096406872264998
3	1.9374456539803833	4.868475369481756
4	0.7076080328076135	2.370800111231532
5	0.31567692474830544	1.3220717784551872
6	0.16684276394398498	0.8384956606847608
7	0.10170505320917173	0.5963246536118293
8	0.06304904863706577	0.4224844393829485
9	0.039211805227018615	0.2955980327217824
>10	0.12911427526517535	1.618832716265871
>50	0.00216149051131036	0.11805029222772785
>100	7.499921713236107E-4	0.11204983940768344
>500	5.8008350273459635E-5	0.028715745842691733
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.390244759072148E-5	0.0	0.0	0.0
3	0.0	4.390244759072148E-5	0.0	0.0	0.0
4	0.0	1.0243904437835013E-4	0.0	0.0	0.0
5	0.0	1.0243904437835013E-4	0.0	1.463414919690716E-5	0.0
6	0.0	1.0243904437835013E-4	0.0	1.463414919690716E-5	0.0
7	0.0	1.0243904437835013E-4	0.0	1.463414919690716E-5	0.0
8	0.0	1.3170734277216444E-4	0.0	2.926829839381432E-5	0.0
9	0.0	2.7804883474123607E-4	0.0	7.31707459845358E-5	1.463414919690716E-5
10-11	0.0	2.7804883474123607E-4	0.0	1.0243904437835013E-4	1.463414919690716E-5
12-13	0.0	2.7804883474123607E-4	0.0	1.3170734277216444E-4	1.463414919690716E-5
14-15	0.0	3.5121958072577183E-4	0.0	1.463414919690716E-4	1.463414919690716E-5
16-17	0.0	3.5853665532422545E-4	0.0	1.463414919690716E-4	1.463414919690716E-5
18-19	0.0	3.8048787911958615E-4	0.0	1.6829271576443232E-4	1.463414919690716E-5
20-21	7.31707459845358E-6	3.9512202831649333E-4	0.0	1.829268649613395E-4	2.926829839381432E-5
22-23	1.463414919690716E-5	4.609756997025756E-4	0.0	1.9756101415824667E-4	5.853659678762864E-5
24-25	1.463414919690716E-5	5.195122964902042E-4	0.0	2.3414638715051457E-4	7.31707459845358E-5
26-27	1.463414919690716E-5	5.634147440809257E-4	0.0	2.560976109458753E-4	7.31707459845358E-5
28-29	2.1951223795360743E-5	5.707318186793793E-4	0.0	3.512195807257719E-4	7.31707459845358E-5
30-31	2.926829839381432E-5	6.000001170731935E-4	0.0	6.365854900654615E-4	7.31707459845358E-5
32-33	2.926829839381432E-5	6.292684154670079E-4	0.0	0.0011780490103510265	1.1707319357525729E-4
34-35	2.926829839381432E-5	6.878050122546365E-4	0.0	0.0019975613653778275	1.1707319357525729E-4
36-37	2.926829839381432E-5	7.463416090422652E-4	0.0	0.0030878054805474108	1.1707319357525729E-4
38-39	2.926829839381432E-5	7.756099074360795E-4	0.0	0.004507317952647406	1.1707319357525729E-4
40-41	2.926829839381432E-5	8.634148026175224E-4	0.0	0.006043903618322657	1.3170734277216444E-4
42-43	2.926829839381432E-5	8.634148026175224E-4	0.0	0.00793170886472368	1.3170734277216444E-4
44-45	2.926829839381432E-5	9.000001756097904E-4	0.0	0.010580489869363878	1.3170734277216444E-4
46-47	2.926829839381432E-5	9.365855486020583E-4	0.0	0.01344878311195768	1.3170734277216444E-4
48-49	2.926829839381432E-5	9.365855486020583E-4	0.0	0.01637561295133911	1.3170734277216444E-4
50-51	2.926829839381432E-5	0.0010170733691850478	0.0	0.019265857417728276	1.39024417370618E-4
52-53	2.926829839381432E-5	0.0010243904437835013	0.0	0.022726833702796823	1.6829271576443232E-4
54-55	3.65853729922679E-5	0.0010317075183819547	0.0	0.02573415136276124	1.9756101415824667E-4
56-57	4.390244759072148E-5	0.0010682928913742227	0.0	0.028763420246521024	2.0487808875670026E-4
58-59	4.390244759072148E-5	0.0010682928913742227	0.0	0.03191707939845452	2.1219516335515382E-4
60-61	4.390244759072148E-5	0.0010756099659726764	0.0	0.035195128818561724	2.1951223795360742E-4
62-63	4.390244759072148E-5	0.001097561189768037	0.0	0.037500007317074605	2.1951223795360742E-4
64-65	4.390244759072148E-5	0.0012146343833432944	0.0	0.039929276083761185	2.1951223795360742E-4
66-67	4.390244759072148E-5	0.0012292685325402015	0.0	0.04335366699583747	2.26829312552061E-4
68-69	4.390244759072148E-5	0.0012512197563355624	0.0	0.047634155635932804	2.3414638715051457E-4
70-71	4.390244759072148E-5	0.0012585368309340158	0.0	0.051702449112672993	2.487805363474217E-4
72-73	4.390244759072148E-5	0.001273170980130923	0.0	0.054775620444023496	2.487805363474217E-4
74-75	4.390244759072148E-5	0.001324390502320098	0.0	0.057475620970852875	2.487805363474217E-4
76-77	4.390244759072148E-5	0.0013536588007139125	0.0	0.059978060483524	2.487805363474217E-4
78-79	4.390244759072148E-5	0.001375610024509273	0.0	0.06164635349197141	2.487805363474217E-4
80-81	4.390244759072148E-5	0.001375610024509273	0.0	0.062202451161453884	2.487805363474217E-4
82-83	4.390244759072148E-5	0.001375610024509273	0.0	0.06266342686115646	2.634146855443289E-4
84-85	4.390244759072148E-5	0.0014268295466984481	0.0	0.06289757324830697	2.634146855443289E-4
86-87	4.390244759072148E-5	0.0014341466212969018	0.0	0.06299269521808687	2.634146855443289E-4
88	4.390244759072148E-5	0.0015219515164783446	0.0	0.06300001229268533	2.634146855443289E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11990	0.0	50.822147	1
GTATCAA	22600	0.0	39.402645	1
TCAACGC	27815	0.0	31.549307	4
ATCAACG	27900	0.0	31.453188	3
CAACGCA	28325	0.0	30.996496	5
TATCAAC	28575	0.0	30.936897	2
AACGCAG	28600	0.0	30.760206	6
ACGCAGA	32545	0.0	26.981035	7
CGCAGAG	32910	0.0	26.655743	8
GTGGTAT	5650	0.0	26.599651	1
TGGTATC	5515	0.0	25.841255	2
GCAGAGT	36735	0.0	23.680351	9
GAGTACT	25980	0.0	21.221733	12-13
CAGAGTA	36755	0.0	19.99513	10-11
GTACATG	20885	0.0	19.871656	1
AGAGTAC	34705	0.0	19.545853	10-11
GTACTTT	28165	0.0	19.496922	14-15
TACATGG	20925	0.0	19.462175	2
ACATGGG	20245	0.0	19.283941	3
AGTACTT	27435	0.0	18.860407	12-13
>>END_MODULE