FastQCFastQC Report
Fri 27 May 2016
SRR2140268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6211486
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC84000.053.3342861
GGTATCA132200.040.6021421
TATACAC119500.040.2619973
TTATACA113650.039.9804462
GTATCAA222100.032.9244041
TACACAT172150.027.677015
TCAACGC263350.027.2673934
ATCAACG264350.027.1803073
CAACGCA268050.026.7734415
TATCAAC270850.026.7475172
AACGCAG272600.026.3907856
ACACATC172650.026.3197636
ACATCTC178100.025.4923578
CACATCT186800.024.1682747
ACGCAGA304400.023.579327
ATACACA212800.023.4476624
CGCAGAG308350.023.2914168
CATCTCC203400.022.0917939
GCAGAGT342700.020.9568379
GAGTACT218950.020.61905912-13
Produced by FastQC (version 0.11.5)