##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6211486
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.585513836785594	33.0	33.0	33.0	33.0	33.0
2	32.63234304963418	33.0	33.0	33.0	33.0	33.0
3	32.66498419218847	33.0	33.0	33.0	33.0	33.0
4	36.58637691528243	37.0	37.0	37.0	37.0	37.0
5	36.59760128252724	37.0	37.0	37.0	37.0	37.0
6	36.57538131777162	37.0	37.0	37.0	37.0	37.0
7	36.552923728718056	37.0	37.0	37.0	37.0	37.0
8	36.53672728232826	37.0	37.0	37.0	37.0	37.0
9	36.57193576545129	37.0	37.0	37.0	37.0	37.0
10-11	36.54846609973845	37.0	37.0	37.0	37.0	37.0
12-13	36.543148773095524	37.0	37.0	37.0	37.0	37.0
14-15	38.59125626299407	40.0	37.0	40.0	37.0	40.0
16-17	38.51880105662317	40.0	37.0	40.0	37.0	40.0
18-19	38.563781275527305	40.0	37.0	40.0	37.0	40.0
20-21	38.588586026978334	40.0	37.0	40.0	37.0	40.0
22-23	38.57365453661147	40.0	37.0	40.0	37.0	40.0
24-25	38.572973290404754	40.0	37.0	40.0	37.0	40.0
26-27	38.49719371282211	40.0	37.0	40.0	37.0	40.0
28-29	38.3787179071074	40.0	37.0	40.0	37.0	40.0
30-31	38.249355234775834	40.0	37.0	40.0	37.0	40.0
32-33	38.118917689189615	40.0	37.0	40.0	35.0	40.0
34-35	38.05254393889629	40.0	37.0	40.0	35.0	40.0
36-37	38.04055787974873	40.0	37.0	40.0	33.0	40.0
38-39	37.986166491652185	40.0	37.0	40.0	33.0	40.0
40-41	37.976429779736975	40.0	37.0	40.0	33.0	40.0
42-43	37.94248332909372	40.0	37.0	40.0	33.0	40.0
44-45	37.84537101366978	40.0	37.0	40.0	33.0	40.0
46-47	37.76681197606675	40.0	37.0	40.0	33.0	40.0
48-49	37.71956524691166	40.0	37.0	40.0	33.0	40.0
50-51	37.6643818954757	40.0	37.0	40.0	33.0	40.0
52-53	37.6467360759644	40.0	37.0	40.0	33.0	40.0
54-55	37.532918900915085	40.0	37.0	40.0	33.0	40.0
56-57	37.37360829128487	40.0	37.0	40.0	33.0	40.0
58-59	37.17711821334271	38.5	37.0	40.0	33.0	40.0
60-61	37.025517551726296	37.0	37.0	40.0	33.0	40.0
62-63	36.9302894089773	37.0	37.0	40.0	33.0	40.0
64-65	36.796076304950404	37.0	37.0	40.0	33.0	40.0
66-67	36.59667337624822	37.0	37.0	40.0	33.0	40.0
68-69	36.40484087931799	37.0	37.0	40.0	33.0	40.0
70-71	36.18542278444049	37.0	37.0	40.0	33.0	40.0
72-73	35.892186465433355	37.0	37.0	37.0	33.0	40.0
74-75	35.55332577858324	37.0	33.0	37.0	33.0	40.0
76-77	33.33114002740898	35.0	33.0	37.0	27.0	37.0
78-79	35.06666393385437	37.0	33.0	37.0	33.0	37.0
80-81	35.28706746181095	37.0	33.0	37.0	33.0	37.0
82-83	35.13353902130909	37.0	33.0	37.0	33.0	37.0
84-85	34.95628574304696	37.0	33.0	37.0	33.0	37.0
86-87	34.82878897299086	37.0	33.0	37.0	33.0	37.0
88-89	34.71905193612605	37.0	33.0	37.0	33.0	37.0
90-91	34.642417002614295	37.0	33.0	37.0	33.0	37.0
92-93	34.562799358126995	37.0	33.0	37.0	33.0	37.0
94-95	34.55396767935399	37.0	33.0	37.0	33.0	37.0
96-97	34.59351266067986	37.0	33.0	37.0	33.0	37.0
98-99	34.60333879833065	37.0	33.0	37.0	33.0	37.0
100	34.531067994344234	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	3.0
20	36.0
21	199.0
22	759.0
23	2233.0
24	5240.0
25	10847.0
26	19004.0
27	30288.0
28	44692.0
29	63625.0
30	86680.0
31	117176.0
32	155463.0
33	210628.0
34	293107.0
35	430924.0
36	757013.0
37	1814984.0
38	2118939.0
39	49642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08180973119798	20.98860079536523	15.542126312447618	23.387463160989174
2	13.690708192484827	20.6066173481569	43.5375831869507	22.16509127240757
3	17.63198049548852	26.520191786635277	31.478071430894317	24.369756286981893
4	11.77581338829388	18.860382201618098	38.97548831310254	30.38831609698549
5	12.662686513339963	37.554894271676694	36.13789035345165	13.644528861531684
6	28.351782488119593	38.47953613676341	18.99345824815511	14.175223126961889
7	25.05120352843104	33.4001235775143	22.863095883980098	18.68557701007456
8	23.378560299419497	37.25179449812814	21.51378913194041	17.85585607051195
9	23.99734942653014	17.803002373345123	21.662320417368726	36.537327782756016
10-11	23.146136045384306	27.267919464038076	28.939033268367666	20.64691122220995
12-13	24.29493444735675	24.618698710457355	29.285619925485435	21.800746916700458
14-15	21.55110133169228	26.29147828830834	26.999041690295716	25.158378689703664
16-17	20.812233980725388	29.944203367760952	27.609045564942107	21.634517086571556
18-19	20.63244608456012	28.075173959983168	30.225770451708335	21.066609503748378
20-21	21.03400462464505	26.978464609793058	30.360460693535764	21.62707007202613
22-23	20.934073493767265	27.470723406646552	30.40477902784667	21.190424071739518
24-25	21.041890169668623	27.698960047225412	30.40560404814113	20.853545734964836
26-27	20.655382901711253	27.729818277134417	30.669795454842035	20.945003366312292
28-29	20.199389646888775	27.276114858457355	31.115805156767216	21.40869033788665
30-31	20.69753854546452	27.157948847065732	30.444009212499175	21.700503394970568
32-33	21.045464305079932	27.28029750720053	30.959640254694243	20.714597933025296
34-35	20.87156087918037	27.997107577315422	30.680642979367285	20.450688564136925
36-37	20.589746769670665	27.590087715062484	30.3510043801752	21.469161135091653
38-39	20.71940411049433	27.37397940090328	30.621754529492968	21.284861959109417
40-41	20.78314174665196	27.349068527892616	30.709955978019938	21.157833747435486
42-43	20.97767330561913	27.366677124387245	30.619218968331353	21.03643060166227
44-45	20.64281295755511	27.75057685437603	30.276295668143216	21.330314519925647
46-47	21.428625099274218	27.408725763561932	29.349894978495477	21.812754158668373
48-49	21.118528843567358	27.509006039471924	29.562312318261363	21.81015279869935
50-51	20.907669984349216	27.960357870648	29.582080563325214	21.549891581677567
52-53	20.960782504095796	28.30789042320103	29.67803721451058	21.053289858192585
54-55	20.89696010307406	28.29456668492169	29.184917331243593	21.62355588076066
56-57	21.38708784055131	28.56714527495431	28.624312066242354	21.421454818252034
58-59	20.849735604256015	28.92815454065075	29.2728000977511	20.94930975734214
60-61	21.357615983263553	29.28516215768094	28.45184274623736	20.905379112818146
62-63	20.929289749365225	29.61684426631678	28.463883078437508	20.989982905880492
64-65	21.0428148540348	29.202776832150175	28.555214313101107	21.19919400071392
66-67	21.20872370579434	28.801837095083155	28.52402470445124	21.465414494671265
68-69	21.23086456177317	28.553133469811158	28.74104482026715	21.474957148148523
70-71	21.32833267178063	28.29867211521664	28.75878257474921	21.614212638253523
72-73	21.388787880670243	28.300808156006173	28.70609507217402	21.60430889114956
74-75	21.25849878206999	28.441030323519584	28.674368183757657	21.62610271065277
76-77	21.386361257759347	28.356244632363893	28.571066579755378	21.686327530121382
78-79	21.385378269650978	28.2439604188261	28.60087867489743	21.769782636625493
80-81	21.465050588297284	28.320802450168053	28.536528229961196	21.677618731573464
82-83	21.54523696768099	28.202490559132887	28.64919516813171	21.60307730505442
84-85	21.599660543941752	28.178099054113975	28.58957495229163	21.632665449652645
86-87	21.48651199227169	28.206232977630936	28.64132574573705	21.665929284360324
88-89	21.580523764570025	28.132795870678734	28.551060917846875	21.735619446904366
90-91	21.58583270713394	28.150769091192384	28.52619807540614	21.737200126267542
92-93	21.691358931880423	28.06785771090712	28.564268063061917	21.676515294150544
94-95	21.570091184478	28.121380775874314	28.58749583825837	21.721032201389313
96-97	21.658354125526742	28.23507233255021	28.622232079904464	21.484341462018584
98-99	22.72309157249085	30.106028244654446	28.88726265924812	18.28361752360658
100	21.76843268828646	30.93790974129858	22.664150370031813	24.62950720038315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1435.0
1	1299.0
2	1804.5
3	2590.0
4	3592.0
5	6181.5
6	8516.5
7	10482.5
8	12275.0
9	12508.5
10	11209.5
11	9822.0
12	10435.0
13	13860.0
14	22621.5
15	35036.5
16	45684.5
17	52461.0
18	54267.5
19	51417.5
20	45693.5
21	39402.0
22	35152.5
23	34907.5
24	38929.5
25	47091.0
26	58068.5
27	70257.0
28	82777.5
29	96899.0
30	111600.5
31	125860.5
32	140553.0
33	154850.5
34	170293.5
35	184740.0
36	199525.0
37	213387.0
38	224478.5
39	233859.0
40	241599.0
41	248384.5
42	252527.0
43	257197.0
44	262448.0
45	266628.0
46	272339.5
47	280299.0
48	296667.0
49	296214.0
50	274985.0
51	258934.0
52	240743.0
53	211115.0
54	176759.0
55	148132.0
56	125354.0
57	106084.5
58	88384.5
59	72710.5
60	58748.5
61	46715.5
62	36237.5
63	27263.0
64	20118.5
65	14864.5
66	11290.5
67	8845.0
68	6872.5
69	5085.0
70	3611.5
71	2634.5
72	1928.5
73	1402.0
74	1035.5
75	716.0
76	496.5
77	330.5
78	210.5
79	144.5
80	99.0
81	76.0
82	65.0
83	44.0
84	26.5
85	18.5
86	12.5
87	10.0
88	7.5
89	4.5
90	3.5
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.024801794610823E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.007816165085134217
14-15	4.588274045856338E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.050705273984822E-6
24-25	4.831406850113574E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0027976278710554665
36-37	0.032121766404100124
38-39	0.13789840442250295
40-41	0.18812136874183832
42-43	0.05923636394981146
44-45	0.007348349380155444
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	2.694558581455024E-5
62-63	0.013659994477913197
64-65	0.0774589641426218
66-67	0.20374090684685434
68-69	0.22058231927627042
70-71	0.08998059316466041
72-73	0.006689190125417537
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	103.0
20-21	531.0
22-23	1000.0
24-25	2048.0
26-27	3517.0
28-29	5565.0
30-31	12366.0
32-33	15596.0
34-35	21708.0
36-37	29902.0
38-39	35917.0
40-41	39500.0
42-43	48377.0
44-45	58700.0
46-47	55844.0
48-49	48547.0
50-51	41885.0
52-53	40932.0
54-55	53381.0
56-57	59431.0
58-59	55529.0
60-61	58397.0
62-63	60204.0
64-65	63134.0
66-67	66862.0
68-69	47882.0
70-71	42314.0
72-73	40981.0
74-75	41685.0
76-77	39014.0
78-79	42727.0
80-81	43595.0
82-83	47580.0
84-85	47599.0
86-87	49948.0
88-89	56868.0
90-91	61189.0
92-93	61141.0
94-95	67604.0
96-97	195349.0
98-99	379681.0
100-101	4067353.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.70670761482965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.49436695718418	78.46468616545671
2	6.903786328598336	11.972091652580685
3	1.5175852786465354	3.9475446910852483
4	0.5290205998362268	1.8347853788888608
5	0.22517179471289844	0.9761952483638866
6	0.11454650839237257	0.5959170366886247
7	0.06790336090300694	0.4121373801916895
8	0.04066745318212497	0.28209127780018267
9	0.024814114037915676	0.1936395117545935
>10	0.08003447290424709	1.0399357969700957
>50	0.0011753344762128535	0.06640167347620846
>100	8.141115362484256E-4	0.1514135178203171
>500	1.1368558964046751E-4	0.06316066892283326
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.609920717844329E-5	0.0	0.0	0.0
3	0.0	1.609920717844329E-5	0.0	1.609920717844329E-5	0.0
4	0.0	6.439682871377317E-5	0.0	1.609920717844329E-5	0.0
5	0.0	8.049603589221645E-5	0.0	1.609920717844329E-5	0.0
6	0.0	8.049603589221645E-5	0.0	1.609920717844329E-5	1.609920717844329E-5
7	0.0	8.049603589221645E-5	1.609920717844329E-5	1.609920717844329E-5	1.609920717844329E-5
8	0.0	9.659524307065974E-5	1.609920717844329E-5	3.219841435688658E-5	1.609920717844329E-5
9	0.0	1.4489286460598962E-4	1.609920717844329E-5	6.439682871377317E-5	1.609920717844329E-5
10-11	0.0	1.4489286460598962E-4	1.609920717844329E-5	1.046448466598814E-4	1.609920717844329E-5
12-13	0.0	1.4489286460598962E-4	1.609920717844329E-5	1.931904861413195E-4	1.609920717844329E-5
14-15	0.0	2.0928969331976277E-4	1.609920717844329E-5	2.0928969331976277E-4	1.609920717844329E-5
16-17	0.0	2.0928969331976277E-4	1.609920717844329E-5	2.2538890049820606E-4	1.609920717844329E-5
18-19	8.049603589221646E-6	2.2538890049820606E-4	1.609920717844329E-5	2.8978572921197924E-4	1.609920717844329E-5
20-21	1.609920717844329E-5	2.4148810767664935E-4	1.609920717844329E-5	3.380833507473091E-4	1.609920717844329E-5
22-23	1.609920717844329E-5	2.7368652203353596E-4	1.609920717844329E-5	3.62232161514974E-4	1.609920717844329E-5
24-25	1.609920717844329E-5	3.219841435688658E-4	1.609920717844329E-5	3.702817651041957E-4	1.609920717844329E-5
26-27	1.609920717844329E-5	3.380833507473091E-4	1.609920717844329E-5	4.668770081748554E-4	1.609920717844329E-5
28-29	1.609920717844329E-5	3.541825579257524E-4	1.609920717844329E-5	7.647123409760563E-4	1.609920717844329E-5
30-31	1.609920717844329E-5	4.1857938663952555E-4	1.609920717844329E-5	0.0013523334029892364	1.609920717844329E-5
32-33	3.219841435688658E-5	4.427281974071905E-4	1.609920717844329E-5	0.002511476319837153	1.609920717844329E-5
34-35	4.829762153532987E-5	5.232242332994069E-4	1.609920717844329E-5	0.003976504173075493	1.609920717844329E-5
36-37	4.829762153532987E-5	5.956706656024017E-4	1.609920717844329E-5	0.00626259159241444	1.609920717844329E-5
38-39	4.829762153532987E-5	5.956706656024017E-4	1.609920717844329E-5	0.009707821928601305	1.609920717844329E-5
40-41	6.439682871377317E-5	6.842163050838398E-4	1.609920717844329E-5	0.013161101868377389	1.609920717844329E-5
42-43	6.439682871377317E-5	7.003155122622831E-4	1.609920717844329E-5	0.016525836168672037	1.609920717844329E-5
44-45	6.439682871377317E-5	7.72761944565278E-4	1.609920717844329E-5	0.020824324485316396	1.609920717844329E-5
46-47	6.439682871377317E-5	8.371587732790511E-4	1.609920717844329E-5	0.026193410079327233	1.609920717844329E-5
48-49	6.439682871377317E-5	8.452083768682728E-4	1.609920717844329E-5	0.030701188089291356	1.609920717844329E-5
50-51	6.439682871377317E-5	9.659524307065974E-4	1.609920717844329E-5	0.0352170157028447	1.609920717844329E-5
52-53	6.439682871377317E-5	9.901012414742622E-4	1.609920717844329E-5	0.039555752037435166	1.609920717844329E-5
54-55	6.439682871377317E-5	0.0010222996558311489	1.609920717844329E-5	0.04358055383204598	1.609920717844329E-5
56-57	6.439682871377317E-5	0.0010786468809557005	1.609920717844329E-5	0.04752485959076459	1.609920717844329E-5
58-59	7.244643230299481E-5	0.0011027956917233654	1.609920717844329E-5	0.0516543062320353	1.609920717844329E-5
60-61	8.049603589221645E-5	0.0011430437096694736	1.609920717844329E-5	0.05593669534150121	2.414881076766494E-5
62-63	8.049603589221645E-5	0.0011430437096694736	1.609920717844329E-5	0.059961497136112034	3.219841435688658E-5
64-65	8.049603589221645E-5	0.0013201349886323497	1.609920717844329E-5	0.06335038024717435	3.219841435688658E-5
66-67	8.049603589221645E-5	0.001336234195810793	1.609920717844329E-5	0.06758447173510493	4.024801794610823E-5
68-69	8.049603589221645E-5	0.0014086806281137878	1.609920717844329E-5	0.0718507616373924	7.244643230299481E-5
70-71	8.049603589221645E-5	0.0014569782496491178	1.609920717844329E-5	0.07569042254945113	8.049603589221645E-5
72-73	8.85456394814381E-5	0.0014811270604167827	1.609920717844329E-5	0.07825824609441284	8.85456394814381E-5
74-75	9.659524307065974E-5	0.0015052758711844476	1.609920717844329E-5	0.0805845815316979	9.659524307065974E-5
76-77	9.659524307065974E-5	0.0015777223034874424	1.609920717844329E-5	0.0828223713295015	1.1269445024910303E-4
78-79	9.659524307065974E-5	0.001609920717844329	1.609920717844329E-5	0.08417470473249075	1.1269445024910303E-4
80-81	9.659524307065974E-5	0.0016179703214335506	1.609920717844329E-5	0.08453693689400571	1.1269445024910303E-4
82-83	9.659524307065974E-5	0.0016260199250227724	1.609920717844329E-5	0.08488306984834225	1.1269445024910303E-4
84-85	9.659524307065974E-5	0.0016260199250227724	1.609920717844329E-5	0.08511650835242968	1.1269445024910303E-4
86-87	9.659524307065974E-5	0.0016582183393796589	1.609920717844329E-5	0.0851970043883219	1.1269445024910303E-4
88	9.659524307065974E-5	0.001770912789628762	1.609920717844329E-5	0.0851970043883219	1.1269445024910303E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	8400	0.0	53.334286	1
GGTATCA	13220	0.0	40.602142	1
TATACAC	11950	0.0	40.261997	3
TTATACA	11365	0.0	39.980446	2
GTATCAA	22210	0.0	32.924404	1
TACACAT	17215	0.0	27.67701	5
TCAACGC	26335	0.0	27.267393	4
ATCAACG	26435	0.0	27.180307	3
CAACGCA	26805	0.0	26.773441	5
TATCAAC	27085	0.0	26.747517	2
AACGCAG	27260	0.0	26.390785	6
ACACATC	17265	0.0	26.319763	6
ACATCTC	17810	0.0	25.492357	8
CACATCT	18680	0.0	24.168274	7
ACGCAGA	30440	0.0	23.57932	7
ATACACA	21280	0.0	23.447662	4
CGCAGAG	30835	0.0	23.291416	8
CATCTCC	20340	0.0	22.091793	9
GCAGAGT	34270	0.0	20.956837	9
GAGTACT	21895	0.0	20.619059	12-13
>>END_MODULE