FastQCFastQC Report
Fri 27 May 2016
SRR2140267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7424670
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144350.053.2670861
GTATCAA260900.038.7808841
GTGGTAT63800.031.7588141
ATCAACG322150.031.081493
TCAACGC322200.031.0766644
TGGTATC61850.030.9911982
TATCAAC328400.030.7165012
CAACGCA328550.030.4627175
AACGCAG335750.029.7723586
ACGCAGA381000.026.2277437
CGCAGAG383850.025.999158
GCAGAGT432150.022.9616379
GAGTACT302550.021.83040812-13
GTACATG244800.020.7729741
CAGAGTA427900.020.55566610-11
GTACTTT327250.020.08664514-15
TACATGG244850.020.0359272
ACATGGG242400.019.6426583
AGAGTAC406150.019.4256110-11
AGTACTT321150.018.5080912-13
Produced by FastQC (version 0.11.5)