FastQCFastQC Report
Fri 27 May 2016
SRR2140266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6805340
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119050.052.8262561
GTATCAA216500.036.629641
TGGTATC50450.030.6506542
TCAACGC256750.030.6010914
ATCAACG257200.030.497173
CAACGCA262800.029.8966145
GTGGTAT55100.029.7101921
TATCAAC265100.029.6700342
AACGCAG268650.029.198016
ACGCAGA302700.025.9156887
CGCAGAG306650.025.5396048
GCAGAGT347600.022.4189939
GAGTACT239100.021.45401612-13
CAGAGTA344550.020.2980310-11
GTACATG202850.020.100871
GTACTTT257500.019.8188114-15
TACATGG202300.019.5149822
ACATGGG200350.018.9717753
AGAGTAC323550.018.6114710-11
ACTTTTT290750.017.6580216-17
Produced by FastQC (version 0.11.5)