##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6805340
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61845109869602	33.0	33.0	33.0	33.0	33.0
2	32.65257797553098	33.0	33.0	33.0	33.0	33.0
3	32.6869029321092	33.0	33.0	33.0	33.0	33.0
4	36.61588884611202	37.0	37.0	37.0	37.0	37.0
5	36.62892140583718	37.0	37.0	37.0	37.0	37.0
6	36.60838562070374	37.0	37.0	37.0	37.0	37.0
7	36.58907034769754	37.0	37.0	37.0	37.0	37.0
8	36.57000311520071	37.0	37.0	37.0	37.0	37.0
9	36.60269332612331	37.0	37.0	37.0	37.0	37.0
10-11	36.58008005478051	37.0	37.0	37.0	37.0	37.0
12-13	36.57573361507287	37.0	37.0	37.0	37.0	37.0
14-15	38.66167032065995	40.0	37.0	40.0	37.0	40.0
16-17	38.59794000005878	40.0	37.0	40.0	37.0	40.0
18-19	38.63310694248928	40.0	37.0	40.0	37.0	40.0
20-21	38.64755505737272	40.0	37.0	40.0	37.0	40.0
22-23	38.639066819300595	40.0	37.0	40.0	37.0	40.0
24-25	38.642585083383246	40.0	37.0	40.0	37.0	40.0
26-27	38.57146505136144	40.0	37.0	40.0	37.0	40.0
28-29	38.466828885401966	40.0	37.0	40.0	37.0	40.0
30-31	38.32990545942947	40.0	37.0	40.0	37.0	40.0
32-33	38.21301732879802	40.0	37.0	40.0	37.0	40.0
34-35	38.16813343836844	40.0	37.0	40.0	37.0	40.0
36-37	38.145782229150214	40.0	37.0	40.0	37.0	40.0
38-39	38.111474477454095	40.0	37.0	40.0	37.0	40.0
40-41	38.08123568615631	40.0	37.0	40.0	37.0	40.0
42-43	38.04117273254528	40.0	37.0	40.0	35.0	40.0
44-45	37.94299373734747	40.0	37.0	40.0	33.0	40.0
46-47	37.859371736991704	40.0	37.0	40.0	33.0	40.0
48-49	37.79378122837664	40.0	37.0	40.0	33.0	40.0
50-51	37.76760890445502	40.0	37.0	40.0	33.0	40.0
52-53	37.723299838526074	40.0	37.0	40.0	33.0	40.0
54-55	37.62662190472537	40.0	37.0	40.0	33.0	40.0
56-57	37.46890990311464	40.0	37.0	40.0	33.0	40.0
58-59	37.257865416536426	38.5	37.0	40.0	33.0	40.0
60-61	37.112693831354164	37.0	37.0	40.0	33.0	40.0
62-63	36.99635028063223	37.0	37.0	40.0	33.0	40.0
64-65	36.82855913408022	37.0	37.0	40.0	33.0	40.0
66-67	36.61488930030673	37.0	37.0	40.0	33.0	40.0
68-69	36.41125590167961	37.0	37.0	40.0	33.0	40.0
70-71	36.18226518040808	37.0	37.0	40.0	33.0	40.0
72-73	35.877491049801066	37.0	37.0	37.0	33.0	40.0
74-75	35.53498384593354	37.0	33.0	37.0	33.0	40.0
76-77	33.30265195424293	35.0	33.0	37.0	27.0	37.0
78-79	35.03739287847861	37.0	33.0	37.0	33.0	37.0
80-81	35.26222290023989	37.0	33.0	37.0	33.0	37.0
82-83	35.10995324071179	37.0	33.0	37.0	33.0	37.0
84-85	34.93037805185699	37.0	33.0	37.0	33.0	37.0
86-87	34.805744179513894	37.0	33.0	37.0	33.0	37.0
88-89	34.68908281094532	37.0	33.0	37.0	33.0	37.0
90-91	34.611651286061225	37.0	33.0	37.0	33.0	37.0
92-93	34.53089548065307	37.0	33.0	37.0	33.0	37.0
94-95	34.52178468428514	37.0	33.0	37.0	33.0	37.0
96-97	34.560957588805735	37.0	33.0	37.0	33.0	37.0
98-99	34.570619281487396	37.0	33.0	37.0	33.0	37.0
100	34.495183562056944	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	7.0
20	31.0
21	164.0
22	629.0
23	1901.0
24	4798.0
25	9809.0
26	18151.0
27	29879.0
28	44685.0
29	64285.0
30	90240.0
31	122642.0
32	166047.0
33	226152.0
34	317406.0
35	472037.0
36	838075.0
37	2013673.0
38	2332979.0
39	51749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.206861082620414	20.586730420522706	15.359320768690472	22.84708772816641
2	14.189377833446676	20.82552816063207	43.04837571098829	21.936718294932962
3	18.277999335815696	26.117666420781326	30.584834850279343	25.019499393123635
4	12.321544551778455	18.292105905068667	39.58516400356191	29.801185539590968
5	13.168835061877878	37.471617876549885	35.772143640141415	13.58740342143082
6	28.955922848821658	38.27641234677474	19.11676712699145	13.65089767741215
7	25.928080007758613	33.04422703347665	23.214769578007857	17.812923380756875
8	23.445294430550128	37.43989279007368	21.266681752858783	17.84813102651741
9	24.788724736750844	17.880135305510084	22.030317368419507	35.300822589319566
10-11	23.716309545151308	27.392209647130052	28.843209891056144	20.048270916662503
12-13	24.57324916394177	24.800080060556816	29.412371971810103	21.21429880369131
14-15	21.623426651163086	26.29829887224812	27.481727868639027	24.596546607949765
16-17	20.729596170066447	29.560227703538693	28.231212547793348	21.47896357860151
18-19	20.651187155968696	28.28644858302451	30.570720933854883	20.491643327151913
20-21	21.406124716015317	27.37190261895947	30.754576175197528	20.46739648982769
22-23	21.124454617752782	27.233970260781938	30.676338282080735	20.965236839384545
24-25	20.868109571728525	27.449243895865717	30.731509813070957	20.9511367193348
26-27	20.805918392433153	27.63191519644948	30.6380527907807	20.924113620336666
28-29	20.744523569525818	27.67187638834213	30.64196498921102	20.94163505292103
30-31	21.006259413121857	27.566840895931882	30.54778552719078	20.87911416375548
32-33	20.803425571530948	27.716496103053917	30.4854638615303	20.99461446388484
34-35	20.96453470748712	27.8019207594319	30.403433412933705	20.830111120147272
36-37	20.946120756655024	27.73428558857727	30.347022187953776	20.972571466813932
38-39	20.861394152525705	27.895607932766847	30.337940058061086	20.90505785664636
40-41	21.1355938003559	27.800853603771277	30.11757764564337	20.94597495022946
42-43	21.04638466415045	27.904284881034858	29.847428255366417	21.201902199448277
44-45	21.11662962751914	28.06648052120729	29.62147130890649	21.195418542367086
46-47	21.242907916003446	28.1222744315006	29.212957969294933	21.42185968320102
48-49	21.210877563430742	28.27193058078593	29.06638100234312	21.45081085344021
50-51	21.239907066414066	28.415913066408926	28.92981795172863	21.414361915448374
52-53	21.3982663695797	28.355267738960848	28.75679256675821	21.489673324701236
54-55	21.38753902760819	28.253332908886097	28.811995824447784	21.547132239057927
56-57	21.373682986059954	28.410589745984826	28.712136914736902	21.503590353218314
58-59	21.393465159181048	28.420655686810324	28.697161952016437	21.48871720199219
60-61	21.465373425200376	28.3543333391435	28.755050126347037	21.425243109309083
62-63	21.393380868325355	28.440830119254468	28.6851154672328	21.480673545187376
64-65	21.42731201866492	28.378226494322384	28.629153461838996	21.5653080251737
66-67	21.540381197732987	28.386797575510432	28.481081641888384	21.591739584868197
68-69	21.422195880734176	28.40118427674156	28.527996737267525	21.648623105256735
70-71	21.470191524157535	28.240836632789406	28.562805807131348	21.72616603592171
72-73	21.549185086263105	28.288461478916314	28.458082971680852	21.704270463139732
74-75	21.395365306485488	28.40309035772307	28.456271219020334	21.74527311677111
76-77	21.58000580159973	28.33908184375349	28.34644639275131	21.734465961895467
78-79	21.568018390134196	28.29636943057774	28.295573624629117	21.840038554658946
80-81	21.582047360076423	28.37154267783842	28.267816973397487	21.77859298868767
82-83	21.646732652725913	28.30334093586305	28.335000737974497	21.71492567343654
84-85	21.72844489209811	28.284328693829924	28.30103227611601	21.686194137955958
86-87	21.577731588178786	28.324850805319173	28.35751991117267	21.739897695329375
88-89	21.690743069820254	28.297778464355105	28.267285709319008	21.74419275650563
90-91	21.71776278508741	28.31501182097379	28.241568061871497	21.725657332067307
92-93	21.69529213170124	28.291292262370344	28.26715885362087	21.746256752307538
94-95	21.64760948098009	28.387026426472318	28.234325392730845	21.731038699816743
96-97	21.74671880486983	28.465216912554514	28.28322364219642	21.504840640379232
98-99	22.819313325427522	30.358329438303393	28.485426954180827	18.33693028208826
100	21.880570553561192	31.205775214853276	22.347146534112863	24.566507697472666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2475.0
1	2003.5
2	2256.0
3	3161.5
4	4113.5
5	6514.0
6	8748.5
7	10640.0
8	12443.5
9	12901.5
10	11915.5
11	10618.0
12	11201.0
13	14690.0
14	23467.0
15	36137.0
16	47416.0
17	54965.5
18	57367.5
19	54690.5
20	48941.0
21	41876.0
22	36313.0
23	35056.5
24	38561.5
25	46539.0
26	57766.5
27	70485.0
28	84420.0
29	100766.5
30	117776.5
31	134516.5
32	153052.5
33	171448.0
34	189335.5
35	206161.0
36	223393.5
37	240544.5
38	253638.0
39	263560.0
40	273974.0
41	282695.5
42	288331.0
43	295231.5
44	299732.0
45	301557.5
46	300782.0
47	295425.5
48	288201.0
49	276845.5
50	262390.0
51	244942.0
52	223976.5
53	201648.0
54	178342.0
55	155816.5
56	134756.5
57	115734.5
58	99139.0
59	83608.5
60	67923.0
61	54162.5
62	41990.5
63	31663.5
64	23824.0
65	17806.0
66	13633.0
67	10760.0
68	8346.5
69	6066.0
70	4368.5
71	3185.0
72	2309.5
73	1653.5
74	1146.0
75	771.0
76	537.5
77	360.5
78	250.0
79	192.5
80	144.0
81	99.0
82	70.5
83	53.0
84	37.0
85	25.0
86	17.0
87	13.5
88	7.5
89	3.5
90	2.5
91	2.5
92	2.0
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.526642313242248E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.007214922399174765
14-15	4.5552463212712366E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.9392233998629E-5
24-25	7.349480975978735E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0026171325778741104
36-37	0.03126364307555922
38-39	0.13483817183998367
40-41	0.18223183092014303
42-43	0.05689241680162411
44-45	0.006974269958035863
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.012079872927816981
64-65	0.07480569413954882
66-67	0.19822156748145578
68-69	0.21376213780269301
70-71	0.08458275951730441
72-73	0.005916192241604237
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	96.0
20-21	481.0
22-23	1124.0
24-25	1947.0
26-27	2950.0
28-29	5069.0
30-31	11321.0
32-33	14775.0
34-35	20101.0
36-37	28554.0
38-39	35104.0
40-41	38816.0
42-43	49094.0
44-45	59069.0
46-47	57397.0
48-49	47455.0
50-51	39038.0
52-53	37620.0
54-55	39620.0
56-57	40702.0
58-59	40357.0
60-61	37799.0
62-63	35985.0
64-65	34950.0
66-67	47865.0
68-69	45451.0
70-71	46559.0
72-73	44380.0
74-75	45258.0
76-77	41706.0
78-79	47244.0
80-81	49608.0
82-83	51212.0
84-85	54344.0
86-87	57228.0
88-89	61733.0
90-91	67943.0
92-93	67880.0
94-95	81722.0
96-97	226449.0
98-99	433715.0
100-101	4655619.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.30024996939717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.79630806476608	79.2654039035247
2	6.761286209428678	11.80523952395523
3	1.4448321906053623	3.784026342110395
4	0.482416277101291	1.6846024632099472
5	0.202636120336317	0.884509197909466
6	0.11624513247699567	0.6088937473780447
7	0.057368689425456636	0.3505810649081354
8	0.034483839231967246	0.24083582278841964
9	0.025744432028591172	0.2022745816274548
>10	0.07695898217106838	1.005060557060541
>50	0.0012909989830790104	0.07500185715813698
>100	4.0466975147154495E-4	0.07587416548953102
>500	2.4393693693879583E-5	0.017696772879938066
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.93886859436854E-5	0.0	0.0	0.0
3	0.0	2.93886859436854E-5	0.0	1.46943429718427E-5	0.0
4	0.0	7.34717148592135E-5	0.0	1.46943429718427E-5	0.0
5	0.0	7.34717148592135E-5	0.0	1.46943429718427E-5	0.0
6	7.34717148592135E-5	7.34717148592135E-5	0.0	1.46943429718427E-5	0.0
7	7.34717148592135E-5	7.34717148592135E-5	0.0	1.46943429718427E-5	0.0
8	7.34717148592135E-5	8.81660578310562E-5	0.0	4.40830289155281E-5	0.0
9	7.34717148592135E-5	1.175547437747416E-4	0.0	5.87773718873708E-5	0.0
10-11	7.34717148592135E-5	1.175547437747416E-4	0.0	8.81660578310562E-5	0.0
12-13	7.34717148592135E-5	1.175547437747416E-4	0.0	8.81660578310562E-5	0.0
14-15	7.34717148592135E-5	2.1306797309171915E-4	0.0	9.551322931697755E-5	7.34717148592135E-6
16-17	1.175547437747416E-4	2.204151445776405E-4	0.0	1.028604008028989E-4	1.46943429718427E-5
18-19	1.175547437747416E-4	2.571510020072473E-4	0.0	1.4694342971842699E-4	2.93886859436854E-5
20-21	1.175547437747416E-4	3.0123403092277535E-4	0.0	1.616377726902697E-4	4.40830289155281E-5
22-23	1.2490191526066296E-4	3.15928373894618E-4	0.0	1.6898494417619104E-4	5.143020040144945E-5
24-25	1.322490867465843E-4	3.6001140281014617E-4	0.0	1.9837363011987645E-4	7.34717148592135E-5
26-27	1.46943429718427E-4	4.040944317256742E-4	0.0	2.644981734931686E-4	7.34717148592135E-5
28-29	1.46943429718427E-4	4.114416032115956E-4	0.0	3.232755453805394E-4	7.34717148592135E-5
30-31	1.46943429718427E-4	4.849133180708091E-4	0.0	5.87773718873708E-4	7.34717148592135E-5
32-33	1.46943429718427E-4	4.996076610426518E-4	0.0	0.0012416719811207083	7.34717148592135E-5
34-35	1.616377726902697E-4	5.583850329300226E-4	0.0	0.0022114986172623264	7.34717148592135E-5
36-37	1.763321156621124E-4	6.31856747789236E-4	0.0	0.003680932914446596	7.34717148592135E-5
38-39	1.763321156621124E-4	6.759397767047643E-4	0.0	0.00576752961644826	7.34717148592135E-5
40-41	1.763321156621124E-4	8.008416919654271E-4	0.0	0.008192096206802306	7.34717148592135E-5
42-43	1.763321156621124E-4	8.302303779091126E-4	0.0	0.010521149567839373	7.34717148592135E-5
44-45	1.763321156621124E-4	9.257436072260901E-4	0.0	0.013364504932890936	7.34717148592135E-5
46-47	1.763321156621124E-4	0.0010139096650571462	0.0	0.016788286845330284	8.081888634513484E-5
48-49	1.763321156621124E-4	0.001028604008028989	0.0	0.019881446040903174	8.81660578310562E-5
50-51	1.910264586339551E-4	0.0010873813799163598	0.0	0.022981952407961984	8.81660578310562E-5
52-53	2.1306797309171915E-4	0.0011020757228882025	0.0	0.026280832405140668	9.551322931697755E-5
54-55	2.2776231606356186E-4	0.001109422894374124	0.0	0.029300519885854345	1.028604008028989E-4
56-57	2.351094875494832E-4	0.0011461587518037306	0.0	0.03279777351315291	1.1020757228882025E-4
58-59	2.351094875494832E-4	0.0011461587518037306	0.0	0.03615543088221897	1.175547437747416E-4
60-61	2.9388685943685397E-4	0.0011902417807192586	0.0	0.03938818633602435	1.175547437747416E-4
62-63	3.673585742960675E-4	0.0012049361236911014	0.0	0.04248869270308317	1.175547437747416E-4
64-65	3.673585742960675E-4	0.0014106569252968991	0.0	0.045310006553676965	1.175547437747416E-4
66-67	3.673585742960675E-4	0.0014180040967828205	0.0	0.049057064011496856	1.2490191526066296E-4
68-69	3.673585742960675E-4	0.0014620871256983486	0.0	0.05317882721509873	1.322490867465843E-4
70-71	3.967472602397529E-4	0.00146943429718427	0.0	0.056778941243200196	1.3959625823250566E-4
72-73	4.261359461834383E-4	0.0014767814686701913	0.0	0.05993087781066045	1.910264586339551E-4
74-75	4.261359461834383E-4	0.001513517326099798	0.0	0.06203951602711988	1.910264586339551E-4
76-77	4.261359461834383E-4	0.0015429060120434835	0.0	0.06429509767329775	1.910264586339551E-4
78-79	4.261359461834383E-4	0.0015429060120434835	0.0	0.0657277961130524	1.9837363011987645E-4
80-81	4.261359461834383E-4	0.0015429060120434835	0.0	0.06605841882991886	2.057208016057978E-4
82-83	4.261359461834383E-4	0.0015429060120434835	1.46943429718427E-5	0.06641108306124308	2.204151445776405E-4
84-85	4.261359461834383E-4	0.0015429060120434835	1.46943429718427E-5	0.06660945669136295	2.204151445776405E-4
86-87	4.261359461834383E-4	0.001550253183529405	1.46943429718427E-5	0.06670496992067992	2.204151445776405E-4
88	4.261359461834383E-4	0.0016016833839308544	1.46943429718427E-5	0.06671231709216585	2.204151445776405E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11905	0.0	52.826256	1
GTATCAA	21650	0.0	36.62964	1
TGGTATC	5045	0.0	30.650654	2
TCAACGC	25675	0.0	30.601091	4
ATCAACG	25720	0.0	30.49717	3
CAACGCA	26280	0.0	29.896614	5
GTGGTAT	5510	0.0	29.710192	1
TATCAAC	26510	0.0	29.670034	2
AACGCAG	26865	0.0	29.19801	6
ACGCAGA	30270	0.0	25.915688	7
CGCAGAG	30665	0.0	25.539604	8
GCAGAGT	34760	0.0	22.418993	9
GAGTACT	23910	0.0	21.454016	12-13
CAGAGTA	34455	0.0	20.29803	10-11
GTACATG	20285	0.0	20.10087	1
GTACTTT	25750	0.0	19.81881	14-15
TACATGG	20230	0.0	19.514982	2
ACATGGG	20035	0.0	18.971775	3
AGAGTAC	32355	0.0	18.61147	10-11
ACTTTTT	29075	0.0	17.65802	16-17
>>END_MODULE