FastQCFastQC Report
Fri 27 May 2016
SRR2140265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8214916
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141250.051.041651
GTATCAA254300.037.0521551
TCAACGC307600.030.1847084
ATCAACG311500.029.7933
CAACGCA314400.029.518195
TATCAAC319000.029.3082162
AACGCAG320550.028.9666776
GTGGTAT67950.028.8966791
TGGTATC65550.028.3160212
ACGCAGA366550.025.3574337
CGCAGAG375000.024.7405098
GCAGAGT419050.021.9962529
GAGTACT270200.021.07207112-13
GTACATG261300.019.9125041
CAGAGTA414400.019.73878510-11
GTACTTT293000.019.31294814-15
TACATGG265650.019.1820952
ACATGGG261600.018.9205283
AGAGTAC387450.018.5552810-11
CATGGGG171350.017.6525344
Produced by FastQC (version 0.11.5)