##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8214916
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.603299412921565	33.0	33.0	33.0	33.0	33.0
2	32.636822214615464	33.0	33.0	33.0	33.0	33.0
3	32.67076766214043	33.0	33.0	33.0	33.0	33.0
4	36.59482458493793	37.0	37.0	37.0	37.0	37.0
5	36.60833646990426	37.0	37.0	37.0	37.0	37.0
6	36.59334130744612	37.0	37.0	37.0	37.0	37.0
7	36.57217286214491	37.0	37.0	37.0	37.0	37.0
8	36.55428698236233	37.0	37.0	37.0	37.0	37.0
9	36.58443932962918	37.0	37.0	37.0	37.0	37.0
10-11	36.564249165785746	37.0	37.0	37.0	37.0	37.0
12-13	36.55737879729993	37.0	37.0	37.0	37.0	37.0
14-15	38.62973814948321	40.0	37.0	40.0	37.0	40.0
16-17	38.56616160165241	40.0	37.0	40.0	37.0	40.0
18-19	38.597907817925346	40.0	37.0	40.0	37.0	40.0
20-21	38.61226870550095	40.0	37.0	40.0	37.0	40.0
22-23	38.60441563638555	40.0	37.0	40.0	37.0	40.0
24-25	38.605950569719454	40.0	37.0	40.0	37.0	40.0
26-27	38.53146188529338	40.0	37.0	40.0	37.0	40.0
28-29	38.42432482267888	40.0	37.0	40.0	37.0	40.0
30-31	38.28904972538923	40.0	37.0	40.0	37.0	40.0
32-33	38.17198490227783	40.0	37.0	40.0	37.0	40.0
34-35	38.13127588078841	40.0	37.0	40.0	37.0	40.0
36-37	38.10940942744325	40.0	37.0	40.0	37.0	40.0
38-39	38.072867843754636	40.0	37.0	40.0	37.0	40.0
40-41	38.04498676548465	40.0	37.0	40.0	37.0	40.0
42-43	38.004916879675946	40.0	37.0	40.0	33.0	40.0
44-45	37.90334317012412	40.0	37.0	40.0	33.0	40.0
46-47	37.81637832376535	40.0	37.0	40.0	33.0	40.0
48-49	37.74958182458667	40.0	37.0	40.0	33.0	40.0
50-51	37.72430628357629	40.0	37.0	40.0	33.0	40.0
52-53	37.678635810263785	40.0	37.0	40.0	33.0	40.0
54-55	37.57430362795391	40.0	37.0	40.0	33.0	40.0
56-57	37.40802822511253	40.0	37.0	40.0	33.0	40.0
58-59	37.18774902491673	38.5	37.0	40.0	33.0	40.0
60-61	37.033750388901495	37.0	37.0	40.0	33.0	40.0
62-63	36.912374510821365	37.0	37.0	40.0	33.0	40.0
64-65	36.73948054492343	37.0	37.0	40.0	33.0	40.0
66-67	36.521701386227754	37.0	37.0	40.0	33.0	40.0
68-69	36.31535811952335	37.0	37.0	40.0	33.0	40.0
70-71	36.08305623641559	37.0	37.0	38.5	33.0	40.0
72-73	35.7837687385628	37.0	35.0	37.0	33.0	40.0
74-75	35.4424869161048	37.0	33.0	37.0	33.0	40.0
76-77	33.19336021621166	35.0	33.0	37.0	27.0	37.0
78-79	34.95210117111766	37.0	33.0	37.0	33.0	37.0
80-81	35.192106869703615	37.0	33.0	37.0	33.0	37.0
82-83	35.04856204954169	37.0	33.0	37.0	33.0	37.0
84-85	34.87810172120475	37.0	33.0	37.0	33.0	37.0
86-87	34.75684971211186	37.0	33.0	37.0	33.0	37.0
88-89	34.64660392565342	37.0	33.0	37.0	33.0	37.0
90-91	34.57748943439541	37.0	33.0	37.0	33.0	37.0
92-93	34.49646698302544	37.0	33.0	37.0	33.0	37.0
94-95	34.49488592712718	37.0	33.0	37.0	33.0	37.0
96-97	34.53537303108689	37.0	33.0	37.0	33.0	37.0
98-99	34.54447592643899	37.0	33.0	37.0	33.0	37.0
100	34.47056044439286	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	34.0
21	207.0
22	852.0
23	2429.0
24	6103.0
25	12917.0
26	22943.0
27	37575.0
28	56515.0
29	80985.0
30	112728.0
31	152341.0
32	205670.0
33	279997.0
34	390690.0
35	577833.0
36	1026056.0
37	2457776.0
38	2729596.0
39	61662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.317062767288185	20.568134841549202	15.159461155780535	22.95534123538208
2	14.381028989790737	20.4555458550155	43.11505251681945	22.048372638374307
3	18.535782958705845	26.000387587651534	30.23374797745954	25.23008147618308
4	12.566287957174485	17.840133727478165	38.914676668635444	30.67890164671191
5	13.294694674905989	37.43046185743104	35.55409452756425	13.720748940098717
6	29.65991374713996	37.77309469749904	18.65481034742169	13.912181207939314
7	26.357007180596852	32.683036564220494	23.14499624828787	17.814960006894776
8	23.66429553266278	37.68208950645387	20.843317204947684	17.810297755935668
9	24.855214587708506	17.77978009756886	22.014065633781282	35.35093968094135
10-11	24.107245892715152	27.230418424241954	28.433735658404785	20.228600024638112
12-13	24.77853907738636	24.611069260057686	29.179879371575186	21.430512290980772
14-15	21.876568610963666	26.005348957679985	27.107581658459296	25.010500772897053
16-17	20.995747248054634	29.336027294740447	27.886584598065273	21.781640859139642
18-19	21.024128548605976	28.079970629036254	30.15997972468617	20.7359210976716
20-21	21.676545645899477	27.146288202031865	30.36522063682576	20.811945515242893
22-23	21.49266714451592	26.97015877074041	30.311262480768093	21.225911603975582
24-25	21.289968502782582	27.162836936569867	30.324388704839194	21.222805855808353
26-27	21.104142876137143	27.306254363606165	30.356111679813342	21.23349108044335
28-29	21.06431205097212	27.292004054905146	30.37704706743032	21.266636826692416
30-31	21.277815538428445	27.278800365479178	30.213626458467008	21.229757637625372
32-33	21.095635793315985	27.457560195638113	30.122224925156566	21.32457908588934
34-35	21.237766697229308	27.429945876369043	30.133339346593512	21.198948079808137
36-37	21.24884622041923	27.38710736587155	30.027609516333385	21.336436897375837
38-39	21.125987252846066	27.56430180586434	30.044199398946585	21.265511542343006
40-41	21.34172040901782	27.464105090618713	29.909582093873606	21.284592406489867
42-43	21.269537267228092	27.63218329794541	29.55062955277023	21.547649882056266
44-45	21.31390926815668	27.73321777547335	29.355146771728442	21.597726184641527
46-47	21.504626462575207	27.774857308532468	29.016935672761836	21.70358055613049
48-49	21.49272992567569	27.985605988340517	28.72167391409779	21.799990171886005
50-51	21.511238373750192	28.09131325913486	28.6215960688668	21.775852298248143
52-53	21.722241310409522	28.004781683228863	28.472413674243075	21.80056333211854
54-55	21.693038991773463	27.894545244651308	28.50201826341453	21.9103975001607
56-57	21.600055791111423	28.073290817706308	28.493725184000713	21.83292820718156
58-59	21.616408273578852	28.05078978044766	28.442209230980364	21.89059271499313
60-61	21.718063331718767	28.04291241325846	28.464469029090683	21.77455522593209
62-63	21.702335185964056	28.146702350358748	28.405705155840344	21.74525730783685
64-65	21.728018975752143	28.044592582656918	28.317442404769434	21.90994603682151
66-67	21.778042803898305	28.01009026055531	28.222080669676767	21.98978626586961
68-69	21.656899115745617	28.079483549392776	28.292196899215366	21.971420435646237
70-71	21.794842596858448	27.90253928157404	28.283095472655162	22.019522648912346
72-73	21.86488131159356	27.983603887709325	28.10472030576177	22.046794494935344
74-75	21.688514451535916	28.12731372964519	28.121231016630706	22.06294080218819
76-77	21.879313412263183	28.035549451666075	28.05962986613158	22.025507269939165
78-79	21.79359719234773	27.929981770788036	28.06267939371662	22.213741643147614
80-81	21.792120434689025	28.088784496377688	28.014000161288173	22.105094907645118
82-83	21.956318298389192	27.974266575449715	28.04322073893049	22.026194387230603
84-85	21.935599186438637	28.03985650382864	27.95363949403506	22.070904815697666
86-87	21.902682277263505	28.062189777428532	28.021762245085576	22.01336570022239
88-89	21.96572152301062	28.002007849538664	27.935051862780753	22.097218764669968
90-91	21.966355156757928	28.046110028994743	27.908710207129968	22.07882460711737
92-93	21.991556300213276	28.003982362847967	27.95627084280851	22.048190494130242
94-95	21.91393847642702	28.03536149415401	27.933827962532465	22.116872066886508
96-97	22.085167299397128	28.11214201501252	27.9201822545413	21.88250843104905
98-99	23.173834077757142	30.03842772423941	28.087403753088097	18.700334444915352
100	22.18593057469586	30.81934660866542	21.94681983994257	25.04790297669615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3311.0
1	2771.0
2	3172.5
3	4307.5
4	5438.5
5	8508.0
6	11354.0
7	13658.5
8	15906.0
9	16497.0
10	15264.0
11	13398.0
12	13623.0
13	17510.0
14	27901.0
15	42975.0
16	56325.0
17	65364.0
18	68114.0
19	64141.0
20	56300.0
21	47326.0
22	40155.0
23	37876.5
24	41141.0
25	49397.0
26	61489.0
27	75386.0
28	91422.0
29	111053.5
30	131777.0
31	152163.0
32	174081.5
33	195799.0
34	216439.5
35	236103.0
36	258807.5
37	279342.5
38	295760.5
39	311557.0
40	324013.0
41	333875.0
42	339221.5
43	347199.0
44	355848.0
45	361983.0
46	364301.5
47	360896.0
48	356358.0
49	347051.0
50	333663.0
51	316154.0
52	293795.0
53	266793.5
54	237185.0
55	209543.0
56	183906.0
57	158793.5
58	136222.0
59	115720.0
60	95302.5
61	77416.5
62	61149.0
63	47227.0
64	35627.0
65	26306.0
66	20256.5
67	15708.0
68	11984.5
69	8744.5
70	6260.0
71	4610.5
72	3251.5
73	2341.5
74	1705.5
75	1136.5
76	751.0
77	503.5
78	345.0
79	243.0
80	184.5
81	137.0
82	103.0
83	68.0
84	40.0
85	27.0
86	22.0
87	20.0
88	18.5
89	15.0
90	9.5
91	7.0
92	4.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.9215149613215764E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.007303787403303941
14-15	5.416975657450423E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.6523496387004427E-5
24-25	4.2619737870346495E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002787999762744284
36-37	0.03173539481604386
38-39	0.13908306394280218
40-41	0.18904539749437632
42-43	0.05870891556657044
44-45	0.006755136884239472
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	6.695731946281213E-6
62-63	0.011995176037805234
64-65	0.0766354335574797
66-67	0.20484064112800018
68-69	0.22178131488452968
70-71	0.08776125098611372
72-73	0.005739053649697848
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	146.0
20-21	591.0
22-23	1273.0
24-25	2791.0
26-27	3885.0
28-29	6519.0
30-31	14892.0
32-33	18977.0
34-35	26008.0
36-37	37147.0
38-39	45311.0
40-41	50141.0
42-43	61910.0
44-45	73081.0
46-47	72472.0
48-49	60898.0
50-51	52680.0
52-53	50447.0
54-55	52003.0
56-57	51086.0
58-59	52527.0
60-61	51980.0
62-63	50642.0
64-65	56912.0
66-67	62638.0
68-69	57645.0
70-71	59208.0
72-73	57822.0
74-75	58219.0
76-77	53768.0
78-79	60912.0
80-81	62183.0
82-83	60117.0
84-85	66544.0
86-87	75342.0
88-89	82493.0
90-91	87068.0
92-93	85232.0
94-95	101067.0
96-97	273528.0
98-99	511412.0
100-101	5505399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.51401347534024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27672780618177	75.45134576845928
2	7.504492369656946	12.684695385095502
3	1.778387556639668	4.5089600977866695
4	0.6713653697879967	2.2695912763655812
5	0.30395637247255197	1.2844286479530396
6	0.15316680458120271	0.7766844831810341
7	0.09358826736442742	0.5536664062419675
8	0.05247058544201979	0.35475998120846813
9	0.03706861099822882	0.28195353804748097
>10	0.1259956406230334	1.621383604581175
>50	0.0022187931874212315	0.12880312892198587
>100	5.376568534648063E-4	0.07249457288434938
>500	2.4166211262310687E-5	0.011233109273489644
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2172979005506569E-5
2	0.0	3.6518937016519705E-5	0.0	0.0	1.2172979005506569E-5
3	0.0	3.6518937016519705E-5	0.0	2.4345958011013138E-5	1.2172979005506569E-5
4	0.0	8.521085303854597E-5	0.0	2.4345958011013138E-5	1.2172979005506569E-5
5	0.0	9.738383204405255E-5	0.0	2.4345958011013138E-5	1.2172979005506569E-5
6	2.4345958011013138E-5	9.738383204405255E-5	0.0	2.4345958011013138E-5	2.4345958011013138E-5
7	2.4345958011013138E-5	1.0955681104955912E-4	0.0	2.4345958011013138E-5	2.4345958011013138E-5
8	2.4345958011013138E-5	1.217297900550657E-4	0.0	2.4345958011013138E-5	2.4345958011013138E-5
9	2.4345958011013138E-5	2.1911362209911823E-4	0.0	4.8691916022026276E-5	2.4345958011013138E-5
10-11	6.695138453028613E-5	2.1911362209911823E-4	1.2172979005506569E-5	4.8691916022026276E-5	2.4345958011013138E-5
12-13	7.303787403303941E-5	2.1911362209911823E-4	2.4345958011013138E-5	6.086489502753285E-5	3.6518937016519705E-5
14-15	7.303787403303941E-5	3.8344883867345695E-4	3.6518937016519705E-5	6.086489502753285E-5	7.303787403303941E-5
16-17	7.303787403303941E-5	4.017083071817168E-4	3.6518937016519705E-5	7.303787403303941E-5	7.303787403303941E-5
18-19	7.303787403303941E-5	4.5040022320374303E-4	3.6518937016519705E-5	7.303787403303941E-5	8.521085303854597E-5
20-21	7.303787403303941E-5	5.234380972367824E-4	3.6518937016519705E-5	7.303787403303941E-5	8.521085303854597E-5
22-23	7.303787403303941E-5	6.695138453028613E-4	3.6518937016519705E-5	8.521085303854597E-5	8.521085303854597E-5
24-25	8.521085303854597E-5	7.668976773469139E-4	3.6518937016519705E-5	1.0347032154680583E-4	9.738383204405255E-5
26-27	8.521085303854597E-5	8.642815093909664E-4	3.6518937016519705E-5	1.3390276906057226E-4	9.738383204405255E-5
28-29	8.521085303854597E-5	8.703679988937196E-4	3.6518937016519705E-5	3.6518937016519703E-4	9.738383204405255E-5
30-31	8.521085303854597E-5	0.0010529626839763182	3.6518937016519705E-5	9.068869359102394E-4	1.0347032154680583E-4
32-33	9.738383204405254E-5	0.0010833951314900847	3.6518937016519705E-5	0.0023493849480627675	1.3390276906057226E-4
34-35	1.0955681104955912E-4	0.0012477303480644235	3.6518937016519705E-5	0.004266629141430053	1.3390276906057226E-4
36-37	1.0955681104955912E-4	0.0014485845016552817	3.6518937016519705E-5	0.006865560159105704	1.3390276906057226E-4
38-39	1.0955681104955912E-4	0.0015094493966828146	3.6518937016519705E-5	0.011308697496115603	1.4607574806607882E-4
40-41	1.0955681104955912E-4	0.0016616116342516468	3.6518937016519705E-5	0.015891824091688826	1.5824872707158539E-4
42-43	1.0955681104955912E-4	0.0016981305712681664	3.6518937016519705E-5	0.02037148036571524	1.5824872707158539E-4
44-45	1.0955681104955912E-4	0.0018137738718204787	3.6518937016519705E-5	0.025325882820956415	1.6433521657433867E-4
46-47	1.0955681104955912E-4	0.0019111577038645312	3.6518937016519705E-5	0.031083701890561026	1.7042170607709195E-4
48-49	1.0955681104955912E-4	0.0019111577038645312	3.6518937016519705E-5	0.03628156392591233	1.8259468508259854E-4
50-51	1.0955681104955912E-4	0.0020572334519306104	3.6518937016519705E-5	0.041582896282810436	1.8259468508259854E-4
52-53	1.217297900550657E-4	0.0020815794099416233	3.6518937016519705E-5	0.0470729098142939	1.947676640881051E-4
54-55	1.217297900550657E-4	0.0021302713259636497	4.8691916022026276E-5	0.052282944828650715	2.0694064309361167E-4
56-57	1.217297900550657E-4	0.0021667902629801694	4.8691916022026276E-5	0.05616003864190455	2.1302713259636495E-4
58-59	1.217297900550657E-4	0.0021728767524829226	4.8691916022026276E-5	0.06011625681869419	2.1911362209911823E-4
60-61	1.217297900550657E-4	0.002227655158007702	4.8691916022026276E-5	0.06412116691150585	2.1911362209911823E-4
62-63	1.3390276906057226E-4	0.002252001116018715	4.8691916022026276E-5	0.06835736360542213	2.1911362209911823E-4
64-65	1.3390276906057226E-4	0.0025137201646371066	6.086489502753285E-5	0.07222837092917322	2.1911362209911823E-4
66-67	1.3390276906057226E-4	0.0025806715491673926	6.086489502753285E-5	0.07667150826618313	2.1911362209911823E-4
68-69	1.3390276906057226E-4	0.0026476229336976787	6.086489502753285E-5	0.08090161847059665	2.1911362209911823E-4
70-71	1.3390276906057226E-4	0.0026658824022059383	6.086489502753285E-5	0.08448047429821559	2.617190486183912E-4
72-73	1.3390276906057226E-4	0.0026902283602169516	6.086489502753285E-5	0.08740807574903992	2.678055381211445E-4
74-75	1.3390276906057226E-4	0.002726747297233471	6.086489502753285E-5	0.09013482304627339	2.678055381211445E-4
76-77	1.4607574806607882E-4	0.0028606500662940433	6.086489502753285E-5	0.0923868241622921	2.678055381211445E-4
78-79	1.4607574806607882E-4	0.002982379856349109	6.086489502753285E-5	0.09399365739101898	2.678055381211445E-4
80-81	1.4607574806607882E-4	0.002994552835354616	6.086489502753285E-5	0.09445014410372547	2.738920276238978E-4
82-83	1.4607574806607882E-4	0.003012812303862876	6.086489502753285E-5	0.09477881453687415	2.799785171266511E-4
84-85	1.4607574806607882E-4	0.003061504219884902	6.086489502753285E-5	0.09496749571145949	2.799785171266511E-4
86-87	1.4607574806607882E-4	0.0030919366673986684	6.086489502753285E-5	0.09507705252250906	2.799785171266511E-4
88	1.4607574806607882E-4	0.003359742205519813	6.086489502753285E-5	0.09509531199101731	2.799785171266511E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14125	0.0	51.04165	1
GTATCAA	25430	0.0	37.052155	1
TCAACGC	30760	0.0	30.184708	4
ATCAACG	31150	0.0	29.793	3
CAACGCA	31440	0.0	29.51819	5
TATCAAC	31900	0.0	29.308216	2
AACGCAG	32055	0.0	28.966677	6
GTGGTAT	6795	0.0	28.896679	1
TGGTATC	6555	0.0	28.316021	2
ACGCAGA	36655	0.0	25.357433	7
CGCAGAG	37500	0.0	24.740509	8
GCAGAGT	41905	0.0	21.996252	9
GAGTACT	27020	0.0	21.072071	12-13
GTACATG	26130	0.0	19.912504	1
CAGAGTA	41440	0.0	19.738785	10-11
GTACTTT	29300	0.0	19.312948	14-15
TACATGG	26565	0.0	19.182095	2
ACATGGG	26160	0.0	18.920528	3
AGAGTAC	38745	0.0	18.55528	10-11
CATGGGG	17135	0.0	17.652534	4
>>END_MODULE