FastQCFastQC Report
Fri 27 May 2016
SRR2140264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724080
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115950.042.0864071
GTATCAA192500.031.2501541
TCAACGC250550.023.560434
ATCAACG251950.023.4130023
TATCAAC257050.023.2246362
CAACGCA255800.023.0280915
AACGCAG256650.022.9842416
GTACATG263950.020.5527781
TACATGG261400.020.4827182
ACGCAGA293600.020.0349667
ACATGGG257750.020.0294193
CGCAGAG298950.019.704258
AGAGTAC306400.017.93532610-11
CATGGGG175050.017.181914
GCAGAGT344250.017.0509389
GAGTACT175550.016.59978712-13
CAGAGTA331300.016.5371079
AGTACTT192700.016.40692112-13
ATGGGGG99600.014.82737355
GTACTTT197050.014.73718614-15
Produced by FastQC (version 0.11.5)