##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6724080
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.889099921476248	31.0	31.0	34.0	30.0	34.0
2	32.015836813363315	33.0	31.0	34.0	30.0	34.0
3	32.07640167874267	33.0	31.0	34.0	30.0	34.0
4	35.782694584240524	37.0	35.0	37.0	35.0	37.0
5	35.65269970018203	37.0	35.0	37.0	33.0	37.0
6	35.65837913885617	37.0	35.0	37.0	33.0	37.0
7	35.533856081426755	37.0	35.0	37.0	33.0	37.0
8	35.52854695363529	37.0	35.0	37.0	33.0	37.0
9	37.13282337509369	39.0	37.0	39.0	33.0	39.0
10-11	37.00402657315202	39.0	37.0	39.0	33.0	39.0
12-13	36.96179217082485	39.0	37.0	39.0	33.0	39.0
14-15	38.10294270740384	40.0	37.0	41.0	33.0	41.0
16-17	38.10813791626512	40.0	37.0	41.0	33.0	41.0
18-19	38.15365276736743	40.0	37.0	41.0	33.0	41.0
20-21	38.143786604573855	40.0	37.0	41.0	33.0	41.0
22-23	38.05198175591924	40.0	37.0	41.0	33.0	41.0
24-25	37.95210579972722	40.0	37.0	41.0	33.0	41.0
26-27	37.78305750829615	40.0	37.0	41.0	32.5	41.0
28-29	37.64657071412447	39.5	37.0	41.0	32.0	41.0
30-31	37.49503899192915	39.0	36.0	41.0	32.0	41.0
32-33	37.63048544607712	39.5	36.5	41.0	32.0	41.0
34-35	37.5929231721031	40.0	36.0	41.0	32.0	41.0
36-37	37.459328452747464	39.5	36.0	41.0	32.0	41.0
38-39	37.296569185056356	39.0	36.0	41.0	31.0	41.0
40-41	37.231336730782964	39.0	36.0	40.5	31.0	41.0
42-43	37.11700016156176	39.0	36.0	40.0	31.0	41.0
44-45	37.178360019625	39.0	35.5	40.5	31.0	41.0
46-47	37.330205253527666	39.0	36.0	41.0	31.0	41.0
48-49	37.2545129673989	39.0	36.0	41.0	31.0	41.0
50-51	37.11210804996813	39.0	35.5	41.0	31.0	41.0
52-53	36.96371755717526	39.0	35.0	40.5	31.0	41.0
54-55	36.81964618103741	39.0	35.0	40.0	31.0	41.0
56-57	36.659644906269754	38.5	35.0	40.0	30.0	41.0
58-59	36.5087062219616	38.0	35.0	40.0	30.0	41.0
60-61	36.256312577121506	38.0	34.5	40.0	30.0	41.0
62-63	35.97425680559162	37.5	34.0	40.0	29.0	41.0
64-65	35.737548120165826	37.0	34.0	40.0	29.0	41.0
66-67	35.466977221452304	37.0	34.0	39.5	29.0	41.0
68-69	35.16876678416622	36.0	34.0	39.0	29.0	40.5
70-71	34.85704398671823	36.0	33.0	39.0	28.5	40.0
72-73	34.52862340954802	35.0	33.0	38.0	28.0	40.0
74-75	34.19174350590521	35.0	33.0	37.5	28.0	39.5
76-77	33.092879778992746	34.0	31.5	36.0	26.5	39.0
78-79	33.48641837403608	35.0	32.0	36.0	27.5	39.0
80-81	33.38262538577278	35.0	32.0	36.0	27.0	38.5
82-83	33.161314345582724	35.0	32.0	36.0	27.0	37.0
84-85	32.96321571569114	34.0	32.0	35.0	27.0	37.0
86-87	32.763559216351155	34.0	32.0	35.0	27.0	36.5
88-89	32.54574182471484	34.0	32.0	35.0	27.0	36.0
90-91	32.423651670209715	34.0	31.0	35.0	26.5	36.0
92-93	32.35611878919714	34.0	31.0	35.0	26.5	35.5
94-95	32.326288594287746	34.0	31.0	35.0	27.0	35.0
96-97	32.23770003348449	34.0	31.0	35.0	27.0	35.0
98-99	32.401698744480456	34.0	31.0	35.0	27.0	35.0
100-101	31.645880360271242	33.0	30.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	10.0
20	48.0
21	306.0
22	1430.0
23	5112.0
24	13034.0
25	27946.0
26	50711.0
27	79849.0
28	113999.0
29	151243.0
30	191793.0
31	237702.0
32	293121.0
33	368320.0
34	480806.0
35	640960.0
36	930904.0
37	1340820.0
38	1244089.0
39	533135.0
40	18739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23015490594996	21.627389918025962	15.11061736326754	24.03183781275654
2	14.295604442509708	22.589936103638138	40.42092209872994	22.693537355122217
3	17.00751626988376	27.924920583931183	31.477480934194713	23.59008221199034
4	12.17653567476889	19.218703525240628	38.8578511855897	29.746909614400778
5	12.603166529844975	38.677633222686225	35.82827687951362	12.890923367955171
6	29.129799169551816	39.10420459007031	18.759339567643455	13.006656672734412
7	25.572479803928566	33.09649795957216	24.01802179629035	17.31300044020892
8	24.67431975824202	36.86342220794517	21.121342994134515	17.34091503967829
9	25.901312893362366	15.881176309621539	21.0000029743846	37.2175078226315
10-11	24.249904819692805	26.73143389132788	29.23715214572105	19.781509143258262
12-13	25.246368276403615	24.459494830519564	29.26427109730997	21.029865795766856
14-15	22.30051546085115	25.509645929257236	26.851078511855896	25.338760098035717
16-17	21.632260472808177	28.608694423623753	27.483373190086972	22.2756719134811
18-19	21.811877470135297	27.761959823470146	29.23482412078985	21.19133858560471
20-21	22.779501163303824	26.897929850169323	28.920195558191743	21.402373428335114
22-23	22.451230894235895	26.89236804813196	28.81002548873239	21.84637556889976
24-25	22.096813533453485	27.154484466193228	28.842110949448664	21.906591050904623
26-27	22.061859584096524	27.205703198017744	28.821956701938824	21.91048051594691
28-29	22.062818704195873	27.280736504194415	28.804899956660623	21.851544834949088
30-31	22.274307192639657	27.269625215000946	28.58721846961101	21.868849122748383
32-33	22.08553626168694	27.486673206127065	28.54219358398738	21.885596948198614
34-35	22.19562958380135	27.44490428452154	28.619446129634856	21.74002000204225
36-37	22.185002257988792	27.521889579855525	28.469859794789933	21.823248367365753
38-39	22.12630644558278	27.48472207392238	28.481738128939643	21.907233351555202
40-41	22.239687342727635	27.413720983847607	28.35962151400839	21.986970159416373
42-43	22.13436362211422	27.614231545896594	28.10903382420284	22.142371007786352
44-45	22.226360027415325	27.70063720462495	27.887737583469992	22.18526518448973
46-47	22.40065955003025	27.69289488049454	27.51407945906515	22.392366110410055
48-49	22.358398169757695	27.835228562154313	27.3721846236112	22.434188644476794
50-51	22.33681927731119	27.8937844283355	27.296085384375644	22.47331090997766
52-53	22.47025225499689	27.78788347811243	27.201696592399234	22.54016767449145
54-55	22.417776192920098	27.663623075289813	27.306675811090418	22.611924920699668
56-57	22.430964724884177	27.743232607697593	27.262762249527643	22.563040417890587
58-59	22.447071689878353	27.768252276076606	27.28330898447001	22.501367049575023
60-61	22.568702188545164	27.695741459091867	27.269349395792307	22.466206956570662
62-63	22.558651860596147	27.801765700568616	27.22227703320778	22.41730540562746
64-65	22.46464873577127	27.65919645452636	27.21720248354132	22.658952326161057
66-67	22.577844900609758	27.703758369759	27.07679060785082	22.641606121780416
68-69	22.47523664100395	27.66714627571631	27.143400732275346	22.7142163510044
70-71	22.527270608288276	27.403876337641737	27.233047156719763	22.835805897350227
72-73	22.563187094509097	27.43786434752079	27.142107418974966	22.85684113899515
74-75	22.452459379616638	27.530214555136023	27.195688574698867	22.821637490548472
76-77	22.679453450841123	27.454152772323265	27.125755948468118	22.7406378283675
78-79	22.516081189135143	27.442816228503915	27.113082284601493	22.928020297759456
80-81	22.63526295419077	27.4682770725126	27.052552202013697	22.843907771282936
82-83	22.59668081079389	27.414613687911903	27.21503175118111	22.77367375011309
84-85	22.659422415027578	27.44340278929996	27.161688535644924	22.73548626002754
86-87	22.539842975228368	27.48001345498443	27.263759615078897	22.716383954708302
88-89	22.5505324390306	27.391371021609107	27.219602856243885	22.838493683116404
90-91	22.648722329188967	27.35099378134876	27.328124907998784	22.67215898146349
92-93	22.70062466554218	27.244678637540137	27.347003634468557	22.707693062449124
94-95	22.392819227307932	27.31022770598841	27.483765107760625	22.81318795894303
96-97	22.3407894783497	27.356796343876127	27.539277699129034	22.76313647864514
98-99	22.4930282590802	28.009825037489893	27.61019899281752	21.886947710612386
100-101	22.86527123046687	30.816597691709358	24.805107742395574	21.513023335428194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1266.0
1	1322.0
2	2512.0
3	3782.0
4	5104.0
5	8416.5
6	11004.0
7	12583.0
8	13256.0
9	12560.0
10	11305.0
11	9914.5
12	10915.5
13	15416.5
14	25736.0
15	39884.5
16	50602.5
17	54585.5
18	53633.5
19	50322.5
20	45389.5
21	40473.5
22	37641.5
23	38694.5
24	44146.0
25	53971.0
26	66314.0
27	77915.0
28	90603.5
29	105140.0
30	117764.0
31	130561.5
32	144939.5
33	158654.5
34	170856.5
35	182624.0
36	195715.5
37	209227.5
38	220145.5
39	227025.0
40	234097.5
41	242483.0
42	249047.5
43	255337.0
44	262703.5
45	270275.5
46	276365.0
47	279285.5
48	281427.5
49	281848.5
50	278441.0
51	273100.0
52	263994.0
53	251471.5
54	238964.5
55	224006.0
56	207800.0
57	190661.0
58	171084.0
59	148983.0
60	124894.5
61	102138.5
62	81322.0
63	62639.5
64	46681.0
65	35130.0
66	27243.0
67	20751.5
68	15468.0
69	11266.0
70	8070.5
71	5852.0
72	4159.0
73	2842.0
74	1932.5
75	1285.5
76	864.5
77	576.0
78	383.5
79	263.0
80	177.5
81	125.0
82	97.0
83	69.0
84	48.5
85	32.0
86	21.5
87	16.5
88	9.5
89	5.5
90	5.5
91	5.5
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0033164388288063197
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	7.43596149956574E-5
20-21	0.0019409090608253382
22-23	5.057852911686913E-4
24-25	7.440738055736634E-6
26-27	2.9776959407676725E-4
28-29	0.003776595669399348
30-31	3.580849616252283E-4
32-33	3.740538028516515E-5
34-35	0.002087256637513648
36-37	0.05582016323605718
38-39	0.031475287793609696
40-41	0.04207242252364926
42-43	8.897016033660739E-4
44-45	0.0022829947073615166
46-47	0.0014085987196628986
48-49	4.642825437848457E-4
50-51	8.090789663498394E-6
52-53	8.576000104545525E-4
54-55	0.002183342791739088
56-57	0.0011230092788849634
58-59	0.003933317163029441
60-61	0.001291797181383537
62-63	1.4609875502375608E-4
64-65	6.950225439249904E-4
66-67	3.515908298431351E-4
68-69	1.6911642188103033E-4
70-71	0.0010644011399555803
72-73	0.001782848177677735
74-75	6.947050875013476E-4
76-77	0.001088544560556907
78-79	1.8075399913466402E-4
80-81	0.00221187011203267
82-83	0.0026297533036275878
84-85	0.003906178767666366
86-87	0.003756480815368968
88-89	0.0699123776546273
90-91	4.836611890284613E-4
92-93	7.531010819803245E-5
94-95	7.73142103629096E-5
96-97	0.008314711962137067
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	256.0
20-21	1127.0
22-23	2147.0
24-25	3025.0
26-27	3830.0
28-29	7332.0
30-31	17645.0
32-33	22322.0
34-35	30552.0
36-37	45639.0
38-39	54278.0
40-41	57804.0
42-43	64741.0
44-45	75819.0
46-47	74131.0
48-49	67815.0
50-51	60767.0
52-53	52199.0
54-55	57065.0
56-57	60730.0
58-59	64724.0
60-61	66702.0
62-63	62308.0
64-65	65106.0
66-67	70474.0
68-69	73488.0
70-71	75350.0
72-73	71144.0
74-75	70419.0
76-77	70158.0
78-79	78138.0
80-81	81527.0
82-83	78554.0
84-85	78197.0
86-87	83947.0
88-89	93701.0
90-91	105761.0
92-93	114935.0
94-95	147745.0
96-97	186239.0
98-99	579715.0
100-101	3646524.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.19472427184336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.92774812958562	76.61369706287824
2	6.985166790956352	11.90198717496727
3	1.7662624093159127	4.514287168601728
4	0.6146988410917623	2.094763931081374
5	0.2830001794574633	1.2055061128880387
6	0.1390499901085377	0.7107795340379649
7	0.09044678338894425	0.5393912140467374
8	0.04417271916496575	0.3010626103677463
9	0.03461625009658415	0.26542096940732923
>10	0.10977062803005092	1.478862709588857
>50	0.003959709443789067	0.22358428333884733
>100	0.0010786653423662841	0.13797550925815724
>500	2.8904017639520344E-5	0.012681719537709277
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.487192299913148E-5	0.0	0.0	0.0
3	0.0	1.487192299913148E-5	0.0	0.0	0.0
4	0.0	7.43596149956574E-5	0.0	0.0	0.0
5	0.0	8.923153799478888E-5	0.0	1.487192299913148E-5	0.0
6	0.0	8.923153799478888E-5	0.0	1.487192299913148E-5	0.0
7	0.0	1.0410346099392035E-4	0.0	1.487192299913148E-5	0.0
8	0.0	1.338473069921833E-4	0.0	1.487192299913148E-5	0.0
9	0.0	2.974384599826296E-4	0.0	1.487192299913148E-5	0.0
10-11	2.974384599826296E-5	2.974384599826296E-4	0.0	1.487192299913148E-5	0.0
12-13	2.974384599826296E-5	2.974384599826296E-4	0.0	1.487192299913148E-5	0.0
14-15	5.948769199652592E-5	4.982094204709045E-4	0.0	2.230788449869722E-5	7.43596149956574E-6
16-17	8.923153799478888E-5	5.056453819704703E-4	0.0	2.974384599826296E-5	1.487192299913148E-5
18-19	8.923153799478888E-5	5.72569035466562E-4	0.0	3.71798074978287E-5	1.487192299913148E-5
20-21	8.923153799478888E-5	6.543646119617851E-4	0.0	6.692365349609167E-5	4.461576899739444E-5
22-23	1.0410346099392037E-4	7.287242269574425E-4	0.0	2.3795076798610368E-4	4.461576899739444E-5
24-25	1.1897538399305184E-4	8.253917264517971E-4	0.0	0.0012789853779253072	4.461576899739444E-5
26-27	1.1897538399305184E-4	8.476996109504943E-4	0.0	0.003539517673793292	4.461576899739444E-5
28-29	1.1897538399305184E-4	8.551355724500602E-4	0.0	0.018247849519934325	4.461576899739444E-5
30-31	1.2641134549261758E-4	9.592390334439804E-4	0.0	0.028070754660860668	4.461576899739444E-5
32-33	1.487192299913148E-4	0.0010112907639409406	0.0	0.03401208789901369	4.461576899739444E-5
34-35	1.6359115299044628E-4	0.00120462576292965	0.0	0.04219908151003557	4.461576899739444E-5
36-37	1.6359115299044628E-4	0.0013830888389192277	0.0	0.05283250645441458	4.461576899739444E-5
38-39	1.6359115299044628E-4	0.001397960761918359	0.0	0.07410679230467217	4.461576899739444E-5
40-41	1.6359115299044628E-4	0.0014723203769140165	0.0	0.12034360090897192	4.461576899739444E-5
42-43	1.6359115299044628E-4	0.0015318080689105426	0.0	0.140599160033789	4.461576899739444E-5
44-45	1.6359115299044628E-4	0.0017028351834005545	0.0	0.1675991362387122	4.461576899739444E-5
46-47	1.6359115299044628E-4	0.0018143746058940406	0.0	0.19904581742037572	5.2051730496960184E-5
48-49	1.6359115299044628E-4	0.0018218105673936062	0.0	0.2253839930518376	5.948769199652592E-5
50-51	1.6359115299044628E-4	0.0020151455663823155	0.0	0.2530532057917217	5.948769199652592E-5
52-53	1.6359115299044628E-4	0.002030017489381447	0.0	0.2875560671497067	8.923153799478888E-5
54-55	1.6359115299044628E-4	0.0020671972968792757	0.0	0.35583306563871936	1.1897538399305184E-4
56-57	1.71027114490012E-4	0.002141556911874933	0.0	0.4237754458602515	1.2641134549261758E-4
58-59	1.7846307598957776E-4	0.0021564288348740646	0.0	0.48059362767843333	1.338473069921833E-4
60-61	1.7846307598957776E-4	0.002193608642371893	0.0	0.516130087684858	1.338473069921833E-4
62-63	1.7846307598957776E-4	0.002230788449869722	0.0	0.5475024092515259	1.338473069921833E-4
64-65	1.7846307598957776E-4	0.0025356628713519174	0.0	0.5611176547572307	1.338473069921833E-4
66-67	1.7846307598957776E-4	0.0025951505633484435	0.0	0.5686131039487929	1.338473069921833E-4
68-69	1.7846307598957776E-4	0.0026992540243423635	0.0	0.5771049719812971	1.338473069921833E-4
70-71	1.7846307598957776E-4	0.0027587417163388896	0.0	0.5854481207838098	1.487192299913148E-4
72-73	1.7846307598957776E-4	0.002795921523836718	0.0	0.5928022867068803	1.7846307598957776E-4
74-75	1.7846307598957776E-4	0.00286284517733281	0.0	0.5994574722489916	1.8589903748914352E-4
76-77	1.7846307598957776E-4	0.0029892565228254275	0.0	0.6057111158701265	1.9333499898870925E-4
78-79	1.7846307598957776E-4	0.003048744214821953	0.0	0.6114368062247921	1.9333499898870925E-4
80-81	1.7846307598957776E-4	0.003048744214821953	0.0	0.6153704298580625	2.0077096048827498E-4
82-83	1.7846307598957776E-4	0.003056180176321519	0.0	0.6184637898418817	2.082069219878407E-4
84-85	1.7846307598957776E-4	0.0030710520993206506	0.0	0.6212894552117167	2.082069219878407E-4
86-87	1.7846307598957776E-4	0.0031007959453189134	0.0	0.6217653567476888	2.082069219878407E-4
88-89	1.7846307598957776E-4	0.0031677195988150052	0.0	0.6218099725166863	2.082069219878407E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11595	0.0	42.086407	1
GTATCAA	19250	0.0	31.250154	1
TCAACGC	25055	0.0	23.56043	4
ATCAACG	25195	0.0	23.413002	3
TATCAAC	25705	0.0	23.224636	2
CAACGCA	25580	0.0	23.028091	5
AACGCAG	25665	0.0	22.984241	6
GTACATG	26395	0.0	20.552778	1
TACATGG	26140	0.0	20.482718	2
ACGCAGA	29360	0.0	20.034966	7
ACATGGG	25775	0.0	20.029419	3
CGCAGAG	29895	0.0	19.70425	8
AGAGTAC	30640	0.0	17.935326	10-11
CATGGGG	17505	0.0	17.18191	4
GCAGAGT	34425	0.0	17.050938	9
GAGTACT	17555	0.0	16.599787	12-13
CAGAGTA	33130	0.0	16.537107	9
AGTACTT	19270	0.0	16.406921	12-13
ATGGGGG	9960	0.0	14.8273735	5
GTACTTT	19705	0.0	14.737186	14-15
>>END_MODULE