FastQCFastQC Report
Fri 27 May 2016
SRR2140263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7336471
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134900.039.612061
GTATCAA226900.031.9979211
ATCAACG284250.025.0648923
TCAACGC293800.024.2501544
AACGCAG295600.024.1302576
TATCAAC298600.024.0530822
ACGCAGA297750.023.9697997
CAACGCA297200.023.945115
CGCAGAG347850.020.5410828
ACATGGG277150.020.4500963
GTACATG302900.019.8502861
TACATGG300150.019.6626952
GCAGAGT394350.017.9732699
AGAGTAC353700.017.8574810-11
GAGTACT200900.017.00696412-13
AGTACTT219200.016.8322312-13
CATGGGG193150.016.531094
CAGAGTA386750.015.7849510-11
GCGCGAT6504.1478415E-815.63222894-95
GTACTTT225650.015.09776314-15
Produced by FastQC (version 0.11.5)