##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7336471
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01812901598057	31.0	31.0	34.0	30.0	34.0
2	32.13299118881544	33.0	31.0	34.0	30.0	34.0
3	32.86677273037677	34.0	31.0	34.0	30.0	37.0
4	35.75532132547106	37.0	35.0	37.0	35.0	37.0
5	35.653743196149755	37.0	35.0	37.0	33.0	37.0
6	35.64631891818287	37.0	35.0	37.0	33.0	37.0
7	35.6046428862051	37.0	35.0	37.0	33.0	37.0
8	35.85473465375928	37.0	35.0	37.0	33.0	39.0
9	37.11817711812669	39.0	37.0	39.0	33.0	39.0
10-11	37.01609765785212	39.0	37.0	39.0	33.0	39.0
12-13	37.11746274196409	39.0	37.0	39.0	33.0	39.5
14-15	38.10979352334385	40.0	37.0	41.0	33.0	41.0
16-17	38.105396177535496	40.0	37.0	41.0	33.0	41.0
18-19	38.15266209053372	40.0	37.0	41.0	33.0	41.0
20-21	38.136956857496926	40.0	37.0	41.0	33.0	41.0
22-23	38.04262090032525	40.0	37.0	41.0	33.0	41.0
24-25	37.930961386685865	40.0	37.0	41.0	33.0	41.0
26-27	37.7648857046013	40.0	37.0	41.0	32.0	41.0
28-29	37.6317179585204	39.5	37.0	41.0	32.0	41.0
30-31	37.50565452367154	39.0	36.0	41.0	32.0	41.0
32-33	37.62078640265821	39.5	36.5	41.0	32.0	41.0
34-35	37.5757784103419	40.0	36.0	41.0	32.0	41.0
36-37	37.43079887203827	39.0	36.0	41.0	31.5	41.0
38-39	37.282631960600426	39.0	36.0	41.0	31.0	41.0
40-41	37.218830610383435	39.0	36.0	40.5	31.0	41.0
42-43	37.11710958665273	39.0	36.0	40.0	31.0	41.0
44-45	37.21294422227055	39.0	36.0	40.5	31.0	41.0
46-47	37.328110849174685	39.0	36.0	41.0	31.0	41.0
48-49	37.252003936904984	39.0	36.0	41.0	31.0	41.0
50-51	37.0945135223769	39.0	35.5	41.0	31.0	41.0
52-53	36.952414224828125	39.0	35.0	40.0	31.0	41.0
54-55	36.80675798614452	39.0	35.0	40.0	31.0	41.0
56-57	36.63440659905815	38.5	35.0	40.0	30.0	41.0
58-59	36.47675736244021	38.0	35.0	40.0	30.0	41.0
60-61	36.21925965156922	38.0	34.5	40.0	30.0	41.0
62-63	35.944901666639694	37.5	34.0	40.0	29.0	41.0
64-65	35.70084626586009	37.0	34.0	40.0	29.0	41.0
66-67	35.427316725346785	36.5	34.0	39.0	29.0	41.0
68-69	35.12543863281371	36.0	34.0	39.0	29.0	40.5
70-71	34.807065307011754	36.0	33.0	39.0	28.5	40.0
72-73	34.47179845076691	35.0	33.0	38.0	28.0	40.0
74-75	34.025484372997425	35.0	32.5	37.0	28.0	39.5
76-77	33.14887448780251	34.0	31.5	36.0	26.5	39.0
78-79	33.46506104241267	35.0	32.0	36.0	27.5	39.0
80-81	33.339519537442236	35.0	32.0	36.0	27.0	38.0
82-83	33.11407746890882	35.0	32.0	35.5	27.0	37.0
84-85	32.920313962196154	34.0	32.0	35.0	27.0	37.0
86-87	32.71960658487985	34.0	32.0	35.0	27.0	36.0
88-89	32.51965733566247	34.0	32.0	35.0	27.0	36.0
90-91	32.402103916817836	34.0	31.0	35.0	26.0	36.0
92-93	32.34611999738448	34.0	31.0	35.0	27.0	35.0
94-95	32.30493096944339	34.0	31.0	35.0	27.0	35.0
96-97	32.24360627843739	34.0	31.0	35.0	27.0	35.0
98-99	32.39403415930359	34.0	31.0	35.0	27.0	35.0
100-101	31.461614390279756	33.0	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	12.0
20	56.0
21	325.0
22	1635.0
23	5543.0
24	14258.0
25	30447.0
26	54447.0
27	86194.0
28	122948.0
29	162528.0
30	206810.0
31	256007.0
32	317186.0
33	397941.0
34	516984.0
35	691179.0
36	1003354.0
37	1455337.0
38	1359734.0
39	619953.0
40	33592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00052600823812	0.010263907833651633	26.496816484735607	30.49239359919263
2	14.723560354581867	25.97531754072862	36.92661003778061	22.374512066908906
3	15.37472171565866	26.508317145941145	34.0520394614795	24.06492167692069
4	12.83750729744587	24.595326554142993	37.19033306340337	25.376833085007767
5	16.786885683866263	37.47140825609479	32.37158573924711	13.370120320791838
6	26.908386879740952	38.43392824697324	20.626606443343128	14.031078429942678
7	25.32672725074494	33.07643416023862	23.275536698775202	18.321301890241234
8	24.336046581524005	31.85400719228632	22.41132010199454	21.398626124195133
9	23.802179549268306	20.20452340096485	23.655092482475567	32.33820456729128
10-11	24.812740348868005	25.857847730877694	29.015994202117067	20.313417718137234
12-13	25.190156139102847	24.130532240909833	28.795411308788655	21.883900311198666
14-15	22.547824597672683	26.375571195985863	26.588984444222874	24.48761976211858
16-17	21.546693647857026	28.594525513567902	27.590622954993744	22.268157883581324
18-19	21.781938805163705	27.736321832562844	29.212011118205076	21.26972824406837
20-21	22.635536535307356	27.106073386871632	28.863596211896496	21.394793865924516
22-23	22.505358124804257	26.896559387820524	28.633649882761407	21.964432604613815
24-25	22.11192751397372	27.330364912529564	28.821188223066663	21.736519350430054
26-27	22.07813350653182	27.52891866869423	28.631339606115358	21.76160821865859
28-29	22.01411046769561	27.299134171672556	28.703570991306073	21.983184369325762
30-31	22.280747863429117	27.31932147439467	28.696391450597076	21.70353921157914
32-33	21.98721681021365	27.804957714739782	28.51935199541345	21.688473479633117
34-35	22.16472933372372	27.36414419575675	28.565166815932574	21.905959654586958
36-37	22.12396653747417	27.60933725927604	28.5105475147587	21.756148688491095
38-39	21.938603286647577	27.749602872740294	28.540193753356004	21.77160008725613
40-41	22.209011674237765	27.375522784585343	28.295117088437244	22.120348452739652
42-43	22.123859639750336	27.54820453918138	28.237440678117014	22.09049514295127
44-45	22.123294861857843	27.940347104328367	27.83254665642439	22.103811377389402
46-47	22.44065751454898	27.543609712764106	27.515969676851036	22.499763095835874
48-49	22.214408860012714	27.765902502101735	27.55567353215051	22.464015105735044
50-51	22.285447740968664	28.02782510969204	27.203064790356567	22.483662358982734
52-53	22.4541519367519	27.687907698390752	27.234180762503506	22.623759602353847
54-55	22.362461927589333	27.650821251103913	27.385028085251705	22.601688736055046
56-57	22.477351759104394	27.983908722740424	27.056496629434	22.482242888721178
58-59	22.432872580306537	27.665926984531335	27.33227152527598	22.56892890988615
60-61	22.51315995920454	27.768742534157205	27.22585748677851	22.492240019859743
62-63	22.361556890991462	28.077310971624676	27.153725883754454	22.407406253629407
64-65	22.502039826763674	27.61513551862853	27.098333595124235	22.784491059483557
66-67	22.42640335468407	27.76008764253808	27.089804364420843	22.72370463835701
68-69	22.451426788531492	27.81852816722379	27.10292959451877	22.62711544972594
70-71	22.543687786619653	27.343740131627147	27.19764400308613	22.914928078667067
72-73	22.43488696372632	27.481342128246578	27.15423627111623	22.929534636910876
74-75	22.379961912269376	27.717023781713007	27.18807466469757	22.714939641320044
76-77	22.784061735015857	27.369204991393996	26.97500528823747	22.87172798535268
78-79	22.449912789789064	27.437578157259416	27.175925262180513	22.936583790771007
80-81	22.497301697987236	27.65624523572922	26.99422139476405	22.852231671519494
82-83	22.69157473215257	27.280854801276032	27.100172817847007	22.927397648724387
84-85	22.555038442625715	27.42731026118404	27.234477911858672	22.783173384331576
86-87	22.513059399717132	27.642448337845337	27.17470866177053	22.669783600667
88-89	22.591688580574342	27.392144112248275	27.129622680519194	22.88654462665819
90-91	22.628045001063025	27.385164965448993	27.295764181983817	22.69102585150416
92-93	22.548819925725162	27.446584209702618	27.260937600914154	22.74365826365807
94-95	22.48563917776204	27.163910841651585	27.446238739552488	22.904211241033888
96-97	22.246156079396226	27.43391156421746	27.59944025493776	22.720492101448546
98-99	22.674187288602134	28.63021361505827	27.59377250674962	21.10182658958998
100-101	26.681860133010364	35.5232104418518	28.493163988131208	9.301765437006617
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1402.0
1	1441.5
2	2929.0
3	4536.0
4	6197.0
5	10164.5
6	12952.0
7	14437.5
8	15019.0
9	13879.5
10	12062.0
11	10253.5
12	11350.0
13	16031.5
14	27205.0
15	44449.0
16	58950.5
17	64919.0
18	63269.5
19	57603.5
20	50477.0
21	44095.0
22	40559.0
23	41626.0
24	47484.5
25	58000.0
26	71016.0
27	84224.0
28	98165.0
29	113215.5
30	128114.0
31	142572.5
32	157839.0
33	173744.5
34	188480.0
35	201676.5
36	217626.5
37	232976.0
38	245008.5
39	255408.5
40	263282.5
41	269600.0
42	274425.5
43	281315.5
44	289558.5
45	298580.5
46	308411.5
47	313809.0
48	316069.0
49	316329.5
50	314283.0
51	309030.5
52	297366.0
53	281467.0
54	264467.0
55	245577.5
56	226679.0
57	206520.5
58	184414.5
59	160146.5
60	134091.0
61	110120.0
62	88341.5
63	68699.0
64	51608.0
65	38565.0
66	29012.0
67	22044.0
68	16837.5
69	12617.0
70	9366.5
71	6766.0
72	4698.0
73	3188.5
74	2214.0
75	1520.5
76	1000.0
77	637.5
78	445.5
79	308.5
80	206.5
81	146.5
82	107.0
83	82.0
84	61.5
85	43.5
86	30.0
87	20.0
88	14.0
89	10.5
90	8.5
91	4.5
92	2.0
93	1.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011449646567130163
2	0.0022899293134260325
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	6.815265813767955E-6
16-17	2.726106325507182E-5
18-19	4.7706860696375685E-4
20-21	0.0016698631482400224
22-23	3.476854951542527E-4
24-25	3.409886420093233E-5
26-27	5.32201790449639E-4
28-29	0.0037213725733066015
30-31	4.241245897449684E-4
32-33	5.352318785753747E-4
34-35	0.004657954225126232
36-37	0.05884893969321473
38-39	0.031431689335529055
40-41	0.03489715319490561
42-43	0.0010969419608720802
44-45	0.0027235786035953113
46-47	0.001314089034788463
48-49	6.283175164870517E-4
50-51	4.48584480457305E-5
52-53	0.001178026898582909
54-55	0.0025689045764005942
56-57	0.0020796496575972373
58-59	0.0035755867350873495
60-61	0.0011674641314394225
62-63	1.837166207867688E-4
64-65	7.76069947831123E-4
66-67	3.6853364720388607E-4
68-69	3.237954539284317E-4
70-71	0.0011368236288875474
72-73	0.001486201515771801
74-75	5.281757062207062E-4
76-77	7.987149641706122E-4
78-79	2.1371732161972438E-4
80-81	0.00237048660932924
82-83	0.0032559223986766603
84-85	0.004729220799187845
86-87	0.015925530849444633
88-89	0.059481980279584665
90-91	8.104814625592652E-4
92-93	1.822145629118049E-4
94-95	0.0013848087933067714
96-97	0.006781640897512858
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	370.0
20-21	1313.0
22-23	2482.0
24-25	3206.0
26-27	4740.0
28-29	10400.0
30-31	20990.0
32-33	27704.0
34-35	39128.0
36-37	56542.0
38-39	63674.0
40-41	68203.0
42-43	76718.0
44-45	89665.0
46-47	87132.0
48-49	78513.0
50-51	69307.0
52-53	60847.0
54-55	66399.0
56-57	71466.0
58-59	78431.0
60-61	80678.0
62-63	74528.0
64-65	77370.0
66-67	83611.0
68-69	84005.0
70-71	85072.0
72-73	79763.0
74-75	78194.0
76-77	79499.0
78-79	87402.0
80-81	90990.0
82-83	87104.0
84-85	86380.0
86-87	93208.0
88-89	104304.0
90-91	116817.0
92-93	129491.0
94-95	168364.0
96-97	245392.0
98-99	882480.0
100-101	3544589.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.41581674810583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.62268405420525	73.0391088873666
2	7.5154670375627415	12.387867082884013
3	2.042177956246314	5.049232926270523
4	0.8117095878138136	2.6759083456777506
5	0.3711167901353276	1.5292946683969204
6	0.19833472971611857	0.9807551243441234
7	0.12156215166357588	0.7013050810507502
8	0.0795661502620577	0.524600740748
9	0.05010777773760734	0.37167060849097167
>10	0.1819308236070529	2.3848328780372747
>50	0.004283839484282471	0.23100479736848836
>100	0.0010591015659355706	0.12441885936459751
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.815265813767955E-5	0.0	1.363053162753591E-5	0.0
3	0.0	6.815265813767955E-5	0.0	1.363053162753591E-5	0.0
4	0.0	8.178318976521545E-5	0.0	1.363053162753591E-5	0.0
5	0.0	8.178318976521545E-5	0.0	1.363053162753591E-5	0.0
6	1.226747846478232E-4	8.178318976521545E-5	0.0	1.363053162753591E-5	1.363053162753591E-5
7	1.226747846478232E-4	9.541372139275136E-5	0.0	1.363053162753591E-5	1.363053162753591E-5
8	1.226747846478232E-4	9.541372139275136E-5	0.0	1.363053162753591E-5	1.363053162753591E-5
9	1.635663795304309E-4	1.9082744278550273E-4	0.0	2.726106325507182E-5	4.0891594882607725E-5
10-11	1.635663795304309E-4	1.9082744278550273E-4	0.0	2.726106325507182E-5	4.0891594882607725E-5
12-13	1.635663795304309E-4	1.9764270859927067E-4	0.0	2.726106325507182E-5	4.0891594882607725E-5
14-15	1.635663795304309E-4	2.1808850604057456E-4	0.0	3.4076329068839774E-5	6.13373923239116E-5
16-17	2.3171903766811046E-4	2.1808850604057456E-4	0.0	5.452212651014363E-5	6.815265813767955E-5
18-19	2.3171903766811046E-4	2.3171903766811046E-4	0.0	3.7483961975723747E-4	8.178318976521545E-5
20-21	2.3171903766811046E-4	2.3171903766811046E-4	0.0	0.0010018440746238893	8.178318976521545E-5
22-23	2.3171903766811046E-4	2.453495692956464E-4	0.0	0.0015675111371666296	1.1585951883405523E-4
24-25	2.3171903766811046E-4	2.5898010092318226E-4	0.0	0.002855596375968773	1.49935847902895E-4
26-27	2.3171903766811046E-4	2.726106325507182E-4	0.0	0.006849342142836794	1.49935847902895E-4
28-29	2.3171903766811046E-4	2.7942589836448613E-4	0.0	0.024180563107248705	1.49935847902895E-4
30-31	2.3171903766811046E-4	3.2031749324709386E-4	0.0	0.03441709235952817	1.49935847902895E-4
32-33	2.3171903766811046E-4	3.543938223159336E-4	0.0	0.0414300008818954	1.49935847902895E-4
34-35	2.3171903766811046E-4	4.089159488260773E-4	0.0	0.050535196009089386	1.7719691115796683E-4
36-37	2.3171903766811046E-4	4.56622809522453E-4	0.0	0.06514031064799411	1.7719691115796683E-4
38-39	2.3171903766811046E-4	4.975144044050608E-4	0.0	0.09476627114044342	1.840121769717348E-4
40-41	2.3171903766811046E-4	5.9974339161158E-4	0.0	0.14442229785955674	1.9764270859927067E-4
42-43	2.3171903766811046E-4	6.338197206804197E-4	0.0	0.16891636319423875	2.0445797441303863E-4
44-45	2.3171903766811046E-4	6.678960497492596E-4	0.0	0.20413765691979152	2.0445797441303863E-4
46-47	2.3171903766811046E-4	6.678960497492596E-4	0.0	0.24287562780524863	2.2490377185434252E-4
48-49	2.3171903766811046E-4	6.951571130043313E-4	0.0	0.275847883812258	2.726106325507182E-4
50-51	2.453495692956464E-4	7.360487078869392E-4	0.0	0.3104694341461992	2.726106325507182E-4
52-53	2.5898010092318226E-4	7.905708343970827E-4	0.0	0.35894641987953063	3.339480248746298E-4
54-55	2.6579536673695026E-4	8.110166318383866E-4	0.0	0.4428968641735243	3.952854171985414E-4
56-57	2.726106325507182E-4	8.246471634659225E-4	0.0	0.5245710096857195	4.021006830123093E-4
58-59	2.726106325507182E-4	8.314624292796905E-4	0.0	0.5889752716258266	4.0891594882607726E-4
60-61	2.726106325507182E-4	8.450929609072264E-4	0.0	0.6277677646377938	4.0891594882607726E-4
62-63	2.862411641782541E-4	8.450929609072264E-4	0.0	0.6510146363285563	4.634380753362209E-4
64-65	2.862411641782541E-4	8.9961508741737E-4	0.0	0.6660150363846595	4.634380753362209E-4
66-67	3.135022274333259E-4	8.9961508741737E-4	0.0	0.6753928421444042	4.634380753362209E-4
68-69	3.135022274333259E-4	9.200608848586739E-4	0.0	0.6848728768913555	4.634380753362209E-4
70-71	3.2031749324709386E-4	9.405066822999777E-4	0.0	0.6940530399425009	4.634380753362209E-4
72-73	3.8165488557100546E-4	9.473219481137457E-4	0.0	0.7018156277043827	5.179602018463645E-4
74-75	3.8165488557100546E-4	9.813982771825856E-4	0.0	0.708971656808839	5.179602018463645E-4
76-77	4.089159488260773E-4	0.001035920403692729	0.0	0.7153643761421533	5.179602018463645E-4
78-79	4.225464804536132E-4	0.001056366201134033	0.0	0.7212050589445524	5.179602018463645E-4
80-81	4.293617462673812E-4	0.001063181466947801	0.0	0.7250147925344488	5.520365309152043E-4
82-83	4.361770120811491E-4	0.0011040730618304085	0.0	0.7283065659224988	5.861128599840441E-4
84-85	4.49807543708685E-4	0.0011313341250854805	0.0	0.7309440737924269	5.861128599840441E-4
86-87	4.634380753362209E-4	0.0011313341250854805	0.0	0.7312984676147428	5.861128599840441E-4
88-89	4.7706860696375685E-4	0.00119948678322316	0.0	0.7313325439438116	5.9974339161158E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13490	0.0	39.61206	1
GTATCAA	22690	0.0	31.997921	1
ATCAACG	28425	0.0	25.064892	3
TCAACGC	29380	0.0	24.250154	4
AACGCAG	29560	0.0	24.130257	6
TATCAAC	29860	0.0	24.053082	2
ACGCAGA	29775	0.0	23.969799	7
CAACGCA	29720	0.0	23.94511	5
CGCAGAG	34785	0.0	20.541082	8
ACATGGG	27715	0.0	20.450096	3
GTACATG	30290	0.0	19.850286	1
TACATGG	30015	0.0	19.662695	2
GCAGAGT	39435	0.0	17.973269	9
AGAGTAC	35370	0.0	17.85748	10-11
GAGTACT	20090	0.0	17.006964	12-13
AGTACTT	21920	0.0	16.83223	12-13
CATGGGG	19315	0.0	16.53109	4
CAGAGTA	38675	0.0	15.78495	10-11
GCGCGAT	650	4.1478415E-8	15.632228	94-95
GTACTTT	22565	0.0	15.097763	14-15
>>END_MODULE