FastQCFastQC Report
Fri 27 May 2016
SRR2140262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24780385
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA343700.051.7182121
GTATCAA650900.038.7967451
TCAACGC816800.030.2949684
ATCAACG821350.030.1323383
CAACGCA829100.029.850685
TATCAAC845550.029.5021212
AACGCAG853250.029.0566716
ACGCAGA972450.025.4642817
CGCAGAG1002600.024.6900128
GTACATG860000.022.5302181
TACATGG878150.021.6563112
GCAGAGT1157500.021.253239
ACATGGG877450.021.192063
GAGTACT642800.019.37777512-13
AGAGTAC1037350.019.3675510-11
CATGGGG642850.018.7948234
CAGAGTA1120250.018.55701810-11
GACCGAG263550.017.5853190-91
AGACCGA264450.017.5615488-89
CGAGACC262950.017.46816886-87
Produced by FastQC (version 0.11.5)