##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24780385
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34472858270765	33.0	31.0	34.0	31.0	34.0
2	32.38681049547858	34.0	31.0	34.0	30.0	34.0
3	32.47600011057133	34.0	31.0	34.0	31.0	34.0
4	36.04829021018035	37.0	35.0	37.0	35.0	37.0
5	35.97808064725387	37.0	35.0	37.0	35.0	37.0
6	36.05782480780666	37.0	35.0	37.0	35.0	37.0
7	35.99563646811783	37.0	35.0	37.0	35.0	37.0
8	35.99705856063173	37.0	35.0	37.0	35.0	37.0
9	37.691715282066845	39.0	37.0	39.0	35.0	39.0
10-11	37.62554754496348	39.0	37.0	39.0	35.0	39.0
12-13	37.61324238909121	39.0	37.0	39.0	35.0	39.0
14-15	38.98708603599177	40.0	38.0	41.0	35.0	41.0
16-17	38.98360249447295	40.0	38.0	41.0	35.0	41.0
18-19	39.01618663309711	40.0	38.5	41.0	35.0	41.0
20-21	39.02921695169843	40.0	39.0	41.0	35.0	41.0
22-23	38.988138992400984	40.0	38.5	41.0	35.0	41.0
24-25	38.950285601286836	40.0	38.0	41.0	35.0	41.0
26-27	38.845273010802146	40.0	38.0	41.0	35.0	41.0
28-29	38.786817080375926	40.0	38.0	41.0	35.0	41.0
30-31	38.6858725782866	40.0	38.0	41.0	34.0	41.0
32-33	38.58782190840055	40.0	38.0	41.0	34.0	41.0
34-35	38.532468785271334	40.0	38.0	41.0	34.0	41.0
36-37	38.46207033369565	40.0	38.0	41.0	34.0	41.0
38-39	38.40284888767313	40.0	38.0	41.0	34.0	41.0
40-41	38.35355978215184	40.0	38.0	41.0	34.0	41.0
42-43	38.28409478366087	40.0	38.0	41.0	33.0	41.0
44-45	38.22901182096984	40.0	38.0	41.0	33.0	41.0
46-47	38.14751485265151	40.0	37.5	41.0	33.0	41.0
48-49	38.05243628503064	40.0	37.0	41.0	33.0	41.0
50-51	37.93681226415604	40.0	37.0	41.0	33.0	41.0
52-53	37.83103495203321	40.0	37.0	41.0	33.0	41.0
54-55	37.67768010346052	40.0	36.0	41.0	32.5	41.0
56-57	37.51148639381465	39.0	36.0	41.0	32.0	41.0
58-59	37.32536174701042	39.0	35.5	41.0	32.0	41.0
60-61	37.117212312630016	39.0	35.0	41.0	31.5	41.0
62-63	36.85488426186793	38.0	35.0	40.0	31.0	41.0
64-65	36.596274830274204	38.0	35.0	40.0	31.0	41.0
66-67	36.327708578595704	37.0	35.0	40.0	31.0	41.0
68-69	36.006476621075734	37.0	34.5	39.5	31.0	41.0
70-71	35.68441055083695	36.0	34.0	39.0	30.5	41.0
72-73	35.361286768024506	36.0	34.0	39.0	30.0	40.5
74-75	35.04210361701077	35.0	34.0	38.0	30.0	40.0
76-77	34.714867096056125	35.0	34.0	37.0	30.0	39.0
78-79	34.40634574636766	35.0	34.0	37.0	29.5	39.0
80-81	34.12836713535689	35.0	33.5	36.0	29.0	39.0
82-83	33.85110727880826	35.0	33.0	36.0	29.0	37.0
84-85	33.651306805202694	35.0	33.0	35.5	29.0	37.0
86-87	33.455705341539314	35.0	33.0	35.0	29.0	36.5
88-89	33.331349456203796	35.0	33.0	35.0	29.0	36.0
90-91	33.206178906884105	35.0	33.0	35.0	29.0	36.0
92-93	33.10497446666243	35.0	33.0	35.0	29.0	36.0
94-95	33.3372248099231	35.0	33.0	35.0	29.0	35.5
96-97	33.40244390030184	35.0	33.0	35.0	30.0	35.0
98-99	33.751776272491306	35.0	34.0	35.0	31.0	35.0
100	33.56539474892762	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	17.0
20	132.0
21	760.0
22	3489.0
23	12009.0
24	31216.0
25	66643.0
26	119922.0
27	189964.0
28	269665.0
29	359826.0
30	456457.0
31	569671.0
32	713252.0
33	913537.0
34	1247938.0
35	1765502.0
36	2840529.0
37	5244556.0
38	6549022.0
39	3281594.0
40	144681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81235682980712	20.013203991786245	13.644106013687843	24.53033316471879
2	14.735530710345124	21.566471806728575	40.56278755884246	23.135209924083842
3	17.479433834462217	27.350975378308284	30.802241369534816	24.36734941769468
4	12.411066252602614	18.455100677410783	38.92015802014375	30.21367504984285
5	12.75830893114605	38.4888839982027	35.62918083531512	13.123626235336133
6	29.133857282685476	38.7386757711795	18.9424256322087	13.185041313926318
7	25.72793360555133	33.02556840823902	23.87888646604966	17.367611520159997
8	24.803468549822774	36.31027524390763	21.184380307247043	17.701875899022554
9	26.20999229834403	15.942839467586964	20.718281818462465	37.12888641560654
10-11	24.264493872875665	26.318299735859636	29.23390011898524	20.183306272279466
12-13	25.492325885977962	24.13119085922192	29.0495325234051	21.326950731395012
14-15	22.617140532723763	25.020194399723817	26.596102118671684	25.76656294888074
16-17	22.031217432658938	28.139195577469845	27.11116070230547	22.718426287565748
18-19	22.184774368921225	27.310449373567035	28.862892969580578	21.64188328793116
20-21	23.07785190766701	26.409576478419382	28.637895604559027	21.87467600935458
22-23	22.89666285185428	26.359372038178698	28.523013280907648	22.220951829059377
24-25	22.556497868898372	26.63068999935418	28.528455869027212	22.284356262720237
26-27	22.519222966620816	26.657688914003618	28.49335928372398	22.32972883565159
28-29	22.520041550294852	26.54997958861975	28.51841268173202	22.411566179353386
30-31	22.807747313843702	26.517758775400534	28.37000318508118	22.30449072567458
32-33	22.64270633990804	26.688589912265982	28.370668586919763	22.298035160906217
34-35	22.66564984762461	26.656144557154565	28.432352606580547	22.245852988640273
36-37	22.777819234756354	26.637945317281147	28.220776615677984	22.363458832284515
38-39	22.640251738718558	26.666051611855806	28.28840268999934	22.40529395942629
40-41	22.763113625633398	26.554389775074227	28.203351304759057	22.47914529453332
42-43	22.66454546870901	26.70922416793641	27.96292879162003	22.66330157173455
44-45	22.68967119732607	26.841585436022765	27.758361438456536	22.710381928194636
46-47	22.94351641148379	26.83592583937684	27.375126560381723	22.845431188757644
48-49	22.909451446231685	26.97747790592228	27.201594164197136	22.9114764836489
50-51	22.861722939036543	27.098822389155313	27.088350884424884	22.951103787383257
52-53	23.011069252097048	27.00477182048307	27.03812807529437	22.946030852125503
54-55	22.95734416210839	26.94629255667976	27.098239238745265	22.998124042466582
56-57	22.933901082893314	27.03972633244816	27.058062815381785	22.968309769276743
58-59	22.84945164643342	27.038318626680724	27.070728075228356	23.0415016516575
60-61	23.125483316485624	27.05424888694532	27.093529498000034	22.72673829856903
62-63	22.916633032161542	27.226046224985318	26.98552597253113	22.871794770322012
64-65	22.84158330637076	27.10263635928366	26.972313729729102	23.083466604616476
66-67	22.89127643469814	27.15527573838228	27.020609238872883	22.932838588046703
68-69	22.807072864905674	27.218961478543875	26.958758857574782	23.015206798975672
70-71	22.902720246814486	26.968755022273278	26.829972700749817	23.298552030162423
72-73	22.959324248107777	26.99245276461743	26.685867959381465	23.36235502789333
74-75	22.849491881352375	27.16005876994882	26.94382841284903	23.046620935849777
76-77	23.094891249586055	26.837493888237045	26.873196895044575	23.194417967132324
78-79	22.860181845352336	26.879381957405297	26.67366577923363	23.586770418008733
80-81	23.155797327754847	27.146924826927105	26.622598189511713	23.074679655806335
82-83	23.167951861670602	26.920169432428093	26.77248398362691	23.139394722274396
84-85	22.995772871910507	26.982911302834196	26.643461130835856	23.377854694419444
86-87	22.918484173995704	27.203144877966363	26.76853013426099	23.109840813776948
88-89	23.15837389734967	27.11832885545502	26.70830421028055	23.014993036914767
90-91	23.231695146237676	27.1325178079516	26.680072622420976	22.95571442338975
92-93	23.131279095923194	26.865198276972567	26.696723096784357	23.306799530319882
94-95	23.021824681746274	27.084552183671455	26.908742565007397	22.984880569574873
96-97	23.335963573812347	27.043835507042896	26.91212288761099	22.70807803153377
98-99	24.10997468473875	29.11654898584144	27.09908337228178	19.674392957138036
100	23.069664658700816	30.022915510043475	20.900887235837153	26.006532595418552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5248.0
1	5450.5
2	8679.5
3	11787.0
4	15192.0
5	27035.0
6	36941.0
7	42082.5
8	43475.0
9	38729.5
10	31115.5
11	23123.5
12	23175.0
13	33126.0
14	61027.0
15	102515.5
16	136778.5
17	154906.0
18	155990.5
19	143033.5
20	122219.5
21	100933.0
22	87397.5
23	88295.0
24	103470.0
25	130869.5
26	166938.5
27	206265.5
28	252199.0
29	306574.5
30	358548.5
31	408698.5
32	461416.0
33	510069.5
34	556841.0
35	600999.5
36	650371.5
37	707177.5
38	754183.0
39	789477.5
40	823106.5
41	855493.5
42	881803.0
43	913104.5
44	949802.5
45	988854.0
46	1023968.0
47	1054447.5
48	1075687.0
49	1071817.0
50	1061206.0
51	1046548.0
52	1013635.0
53	967356.0
54	919857.0
55	866812.5
56	806685.5
57	736271.0
58	660457.0
59	581220.0
60	487339.0
61	398148.5
62	319461.5
63	249239.0
64	190043.0
65	142033.0
66	106869.5
67	81159.0
68	61524.0
69	46090.5
70	34282.0
71	25234.5
72	18588.0
73	13483.5
74	9403.0
75	6519.5
76	4296.0
77	2710.5
78	1889.5
79	1339.0
80	906.5
81	643.5
82	493.0
83	358.0
84	243.5
85	165.0
86	125.5
87	101.0
88	65.5
89	36.5
90	27.0
91	23.0
92	18.0
93	11.0
94	5.5
95	4.0
96	3.5
97	3.0
98	3.0
99	4.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.877989587328849E-5
3	0.0
4	0.0
5	0.030968041860527994
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.035694914378697E-6
22-23	2.0384554828670138E-4
24-25	9.286584960735006E-5
26-27	1.4742988411425427E-4
28-29	1.21255577251322E-4
30-31	4.047374109210902E-6
32-33	1.4197852757314276E-5
34-35	2.0340173537072172E-5
36-37	5.634941422436022E-4
38-39	5.131155410998364E-5
40-41	0.0
42-43	4.160190376967955E-6
44-45	2.9365013876437306E-5
46-47	4.2372170365276924E-6
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.0387872302208967E-4
70-71	1.0491898925055993E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	1.022122904810946E-5
90-91	0.0
92-93	0.0
94-95	0.0012524623116539995
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	865.0
20-21	4243.0
22-23	6694.0
24-25	8319.0
26-27	13501.0
28-29	26836.0
30-31	52920.0
32-33	65594.0
34-35	84610.0
36-37	120156.0
38-39	153275.0
40-41	162616.0
42-43	186870.0
44-45	234362.0
46-47	226079.0
48-49	200682.0
50-51	169764.0
52-53	144156.0
54-55	152906.0
56-57	175988.0
58-59	111050.0
60-61	196686.0
62-63	181360.0
64-65	188039.0
66-67	202134.0
68-69	207556.0
70-71	218590.0
72-73	194052.0
74-75	190458.0
76-77	199095.0
78-79	215434.0
80-81	225205.0
82-83	215563.0
84-85	206585.0
86-87	213852.0
88-89	234787.0
90-91	254655.0
92-93	261037.0
94-95	304740.0
96-97	892737.0
98-99	1497772.0
100-101	1.6178562E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.65284173540856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.2308914818123	65.23611985976736
2	8.469973385048814	12.815551120044411
3	2.3946211210014696	5.434796780501724
4	1.076379951357809	3.257248084289563
5	0.566958980770599	2.1446029021353334
6	0.3423417397463128	1.5539475273871335
7	0.21745006632415942	1.151550081708301
8	0.14124458235816637	0.854844322810099
9	0.10661464520440504	0.7259130792293031
>10	0.43511822052268834	5.34333783059178
>50	0.01302619568586195	0.6700781021774759
>100	0.005211112375861308	0.7230958464201039
>500	1.3483008539926213E-4	0.06110955730142367
>1k	3.368770617057192E-5	0.027804905635927094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.035449812422204E-6
2	0.0	4.842539774906645E-5	0.0	0.0	4.035449812422204E-6
3	0.0	5.2460847561488653E-5	0.0	0.0	4.035449812422204E-6
4	0.0	1.0088624531055511E-4	0.0	0.0	1.2106349437266612E-5
5	0.0	1.0088624531055511E-4	0.0	0.0	1.2106349437266612E-5
6	1.0492169512297731E-4	1.0088624531055511E-4	0.0	0.0	2.4212698874533224E-5
7	1.0492169512297731E-4	1.0088624531055511E-4	0.0	0.0	2.4212698874533224E-5
8	1.0492169512297731E-4	1.089571449353995E-4	0.0	0.0	2.824814868695543E-5
9	1.0492169512297731E-4	2.945878363068209E-4	0.0	0.0	3.228359849937763E-5
10-11	1.2106349437266613E-4	2.96605561213032E-4	0.0	4.035449812422204E-6	3.228359849937763E-5
12-13	1.3316984380993275E-4	2.9862328611924313E-4	0.0	8.070899624844408E-6	3.4301323405588736E-5
14-15	1.3922301852856606E-4	4.2977540502296474E-4	0.0	8.070899624844408E-6	4.842539774906645E-5
16-17	2.0984339024595461E-4	4.4188175446023136E-4	0.0	1.2106349437266612E-5	5.447857246769975E-5
18-19	2.1186111515216573E-4	4.7416535295960894E-4	0.0	1.4124074343477713E-5	6.053174718633306E-5
20-21	2.138788400583768E-4	5.084666763651977E-4	0.0	2.017724906211102E-5	6.254947209254416E-5
22-23	2.158965649645879E-4	5.568920741142642E-4	0.0	2.8248148686955426E-5	7.667354643602187E-5
24-25	2.17914289870799E-4	6.053174718633306E-4	0.0	6.658492190496636E-5	8.877989587328849E-5
26-27	2.1993201477701012E-4	6.295301707378638E-4	0.0	1.1702804456024393E-4	9.68507954981329E-5
28-29	2.2194973968322123E-4	6.31547895644075E-4	0.0	5.629452488328975E-4	9.68507954981329E-5
30-31	2.2194973968322123E-4	7.183100666111524E-4	0.0	0.0022679227945812787	9.8868520404344E-5
32-33	2.2396746458943235E-4	7.243632413297856E-4	0.0	0.005500318094331464	1.0492169512297731E-4
34-35	2.3809153893291005E-4	8.454267357024518E-4	0.0	0.009275481393852435	1.089571449353995E-4
36-37	2.4212698874533225E-4	9.564016055440623E-4	0.0	0.01633550084068508	1.089571449353995E-4
38-39	2.4212698874533225E-4	9.745611296999623E-4	0.0	0.04444442650911194	1.089571449353995E-4
40-41	2.461624385577545E-4	0.0010855359995415729	0.0	0.07642536627255792	1.1299259474782172E-4
42-43	2.5625106308881E-4	0.001123872772759584	0.0	0.09205869884588153	1.1904576946645501E-4
44-45	2.643219627136543E-4	0.0012792375905378386	0.0	0.10990951109113115	1.3316984380993272E-4
46-47	2.7037513743228766E-4	0.0013962656350980827	0.0	0.1291969434696031	1.5334709287204376E-4
48-49	2.7037513743228766E-4	0.0014103897094415604	0.0	0.14767728588559056	1.6545344230931036E-4
50-51	3.006410110254542E-4	0.0016545344230931036	0.0	0.166405808465042	1.6545344230931036E-4
52-53	3.107296355565097E-4	0.0016747116721552147	0.0	0.23175386500250095	1.8764841627763248E-4
54-55	3.107296355565097E-4	0.001680764846873848	0.0	0.30617966589300366	2.1589656496458792E-4
56-57	3.107296355565097E-4	0.0017069952706545923	0.0	0.36846683374774036	2.2194973968322123E-4
58-59	3.107296355565097E-4	0.0017170838951856477	0.0	0.5462687524830627	2.2598518949564343E-4
60-61	3.3494233443104294E-4	0.001741296594060181	0.0	0.7119723926807433	2.3405608912048783E-4
62-63	3.8336773218010937E-4	0.0017735801925595589	0.0	0.7191312806479803	2.4212698874533225E-4
64-65	3.874031819925316E-4	0.0019814058578993023	0.0	0.7250855868462092	2.4212698874533225E-4
66-67	4.075804310546426E-4	0.002005618556773835	0.0	0.7307594292824748	2.4414471365154336E-4
68-69	4.075804310546426E-4	0.002041937605085635	0.0	0.7358642732951889	2.461624385577545E-4
70-71	4.2372223030433145E-4	0.0020661503039601688	0.0	0.7405010051296621	2.461624385577545E-4
72-73	4.3986402955402024E-4	0.0020802743783036462	0.0	0.7442015126076532	2.945878363068209E-4
74-75	4.4389947936644247E-4	0.0021609833745520903	0.0	0.7469012285321637	2.945878363068209E-4
76-77	4.479349291788647E-4	0.0022840645938309676	0.0	0.7495000582113636	2.945878363068209E-4
78-79	4.5197037899128686E-4	0.0023445963410173008	0.0	0.7513220638016722	2.945878363068209E-4
80-81	4.5197037899128686E-4	0.0023445963410173008	0.0	0.751989930745628	2.9862328611924313E-4
82-83	4.53988103897498E-4	0.0023445963410173008	0.0	0.7524883087974621	3.147650853689319E-4
84-85	4.6004127861613126E-4	0.0023587204153607782	0.0	0.7526416558903342	3.1678281027514303E-4
86-87	4.681121782409757E-4	0.002397057188578789	0.0	0.7527183294367703	3.2081826008756526E-4
88	4.761830778658201E-4	0.0025423333818259887	0.0	0.7527243826114889	3.309068846186207E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	34370	0.0	51.718212	1
GTATCAA	65090	0.0	38.796745	1
TCAACGC	81680	0.0	30.294968	4
ATCAACG	82135	0.0	30.132338	3
CAACGCA	82910	0.0	29.85068	5
TATCAAC	84555	0.0	29.502121	2
AACGCAG	85325	0.0	29.056671	6
ACGCAGA	97245	0.0	25.464281	7
CGCAGAG	100260	0.0	24.690012	8
GTACATG	86000	0.0	22.530218	1
TACATGG	87815	0.0	21.656311	2
GCAGAGT	115750	0.0	21.25323	9
ACATGGG	87745	0.0	21.19206	3
GAGTACT	64280	0.0	19.377775	12-13
AGAGTAC	103735	0.0	19.36755	10-11
CATGGGG	64285	0.0	18.794823	4
CAGAGTA	112025	0.0	18.557018	10-11
GACCGAG	26355	0.0	17.58531	90-91
AGACCGA	26445	0.0	17.56154	88-89
CGAGACC	26295	0.0	17.468168	86-87
>>END_MODULE