FastQCFastQC Report
Fri 27 May 2016
SRR2140261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25756509
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA394150.053.3001561
GTATCAA760100.040.0122871
ATCTCGT243900.038.16143894
TATCTCG297550.032.68955294
ATCAACG966300.030.8842643
TCAACGC967800.030.831974
TATCAAC991100.030.3794982
CAACGCA986300.030.266695
AACGCAG1011250.029.607046
TATGCCG144700.027.43600386-87
ACGCAGA1151850.025.9633227
CTCGTAT149350.025.93330882-83
TCTCGTA151050.025.92674482-83
ATGCCGT158850.025.86311788-89
CCGTCTT158550.025.687490-91
CGCAGAG1180850.025.3220718
TGCCGTC163050.024.8526988-89
GCCGTCT169750.024.59911390-91
TCGTATG165900.024.45091684-85
GTACATG1104000.023.7913741
Produced by FastQC (version 0.11.5)