##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140261_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25756509
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42272724149068	34.0	31.0	34.0	31.0	34.0
2	32.46128984327806	34.0	31.0	34.0	31.0	34.0
3	32.551652516262976	34.0	31.0	34.0	31.0	34.0
4	36.10398462773041	37.0	35.0	37.0	35.0	37.0
5	36.04299651012488	37.0	35.0	37.0	35.0	37.0
6	36.12037201936023	37.0	36.0	37.0	35.0	37.0
7	36.06264979465967	37.0	35.0	37.0	35.0	37.0
8	36.064177563815036	37.0	35.0	37.0	35.0	37.0
9	37.775314581646136	39.0	38.0	39.0	35.0	39.0
10-11	37.71011865389056	39.0	37.5	39.0	35.0	39.0
12-13	37.702234491483296	39.0	37.5	39.0	35.0	39.0
14-15	39.09622082324899	40.0	38.0	41.0	36.0	41.0
16-17	39.0935356379236	40.0	38.0	41.0	36.0	41.0
18-19	39.13417410333054	40.5	38.5	41.0	36.0	41.0
20-21	39.14921702778345	41.0	39.0	41.0	36.0	41.0
22-23	39.10526996823039	40.0	39.0	41.0	35.0	41.0
24-25	39.07066604902475	40.5	39.0	41.0	35.0	41.0
26-27	38.97494716454591	40.0	38.0	41.0	35.0	41.0
28-29	38.919827687487924	40.0	38.0	41.0	35.0	41.0
30-31	38.816842231729495	40.0	38.0	41.0	35.0	41.0
32-33	38.71703322472415	40.0	38.0	41.0	35.0	41.0
34-35	38.6647568109707	40.0	38.0	41.0	34.5	41.0
36-37	38.59842552188592	40.0	38.0	41.0	34.0	41.0
38-39	38.544883348927726	40.0	38.0	41.0	34.0	41.0
40-41	38.497897709804995	40.0	38.0	41.0	34.0	41.0
42-43	38.42551518767582	40.0	38.0	41.0	34.0	41.0
44-45	38.37397578175149	40.0	38.0	41.0	34.0	41.0
46-47	38.30019175824107	40.0	38.0	41.0	33.0	41.0
48-49	38.20815527051264	40.0	38.0	41.0	33.0	41.0
50-51	38.10027561154072	40.0	37.0	41.0	33.0	41.0
52-53	37.9995189851016	40.0	37.0	41.0	33.0	41.0
54-55	37.86007046250849	40.0	37.0	41.0	33.0	41.0
56-57	37.69852886600219	39.5	36.0	41.0	33.0	41.0
58-59	37.52615931349028	39.0	36.0	41.0	32.0	41.0
60-61	37.32621372063595	39.0	35.5	41.0	32.0	41.0
62-63	37.083549775509574	39.0	35.0	40.5	32.0	41.0
64-65	36.8484036691473	38.0	35.0	40.0	31.0	41.0
66-67	36.58279873744702	37.5	35.0	40.0	31.0	41.0
68-69	36.2653361378988	37.0	35.0	40.0	31.0	41.0
70-71	35.94375158768798	36.5	34.5	39.0	31.0	41.0
72-73	35.630672405062406	36.0	34.0	39.0	31.0	41.0
74-75	35.30221127847658	35.5	34.0	38.5	30.0	40.0
76-77	34.964331508373775	35.0	34.0	37.5	30.0	39.5
78-79	34.63465337460609	35.0	34.0	37.0	30.0	39.0
80-81	34.3338542944649	35.0	34.0	36.5	30.0	39.0
82-83	34.031309410187575	35.0	33.5	36.0	29.0	37.5
84-85	33.81746956572765	35.0	33.0	36.0	29.0	37.0
86-87	33.603402237098	35.0	33.0	35.0	29.0	37.0
88-89	33.45988604449347	35.0	33.0	35.0	29.0	36.0
90-91	33.33887519416682	35.0	33.0	35.0	29.0	36.0
92-93	33.21684272311997	35.0	33.0	35.0	29.0	36.0
94-95	33.42593911181011	35.0	33.0	35.0	30.0	36.0
96-97	33.476481097429385	35.0	33.0	35.0	30.0	35.5
98-99	33.81734788807097	35.0	34.0	35.0	31.0	35.0
100	33.61282497792108	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	24.0
20	103.0
21	669.0
22	3096.0
23	10713.0
24	28590.0
25	61323.0
26	110954.0
27	177793.0
28	256537.0
29	343932.0
30	440490.0
31	552100.0
32	691856.0
33	888532.0
34	1216753.0
35	1737847.0
36	2821603.0
37	5327740.0
38	7119540.0
39	3774921.0
40	191391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0324159613401	20.16233255834477	13.726044938776447	24.079206541538685
2	14.488278488256745	21.809889468634896	41.17738092418649	22.52445111892187
3	17.316702352791676	27.99775388815309	30.903019504700737	23.782524254354502
4	11.973408352816758	18.635087542337356	39.62413539816285	29.76736870668304
5	12.374968182545851	38.95147303258612	35.76180092577238	12.911757859095651
6	28.339422862003545	39.26136496215384	19.404644472587492	12.994567703255127
7	25.339132721752005	33.43199577240844	24.183541333183005	17.045330172656552
8	24.565413736776208	36.42603506554401	21.525836440023763	17.482714757656016
9	26.316862273532486	15.963133823764702	21.002628888876206	36.717375013826604
10-11	23.958821438107158	26.68192145138924	29.492706096156123	19.86655101434748
12-13	25.208150297076365	24.509356062189948	29.38916721982781	20.89332642090588
14-15	22.399473857268468	25.354521064947118	27.12782427152686	25.118180806257556
16-17	21.572950744217703	28.49191829529382	27.575231565737425	22.35989939475105
18-19	21.71411117865391	27.59252234066348	29.46443363112602	21.228932849556593
20-21	22.875311946158277	26.680981790538866	29.136306515164183	21.30739974813867
22-23	22.47356817291645	26.638892504372354	29.02596144098053	21.861577881730668
24-25	22.06605211993373	26.883222524575075	29.159088746064395	21.891636609426804
26-27	22.05644073344484	26.95795016094008	29.053243149840775	21.932365955774305
28-29	22.101132131939096	26.92810491066229	29.071240459323633	21.899522498074976
30-31	22.304810150008702	26.862737770950034	28.92179125603482	21.910660823006445
32-33	22.12487095104795	27.072352471300487	28.850723801537566	21.952052776114
34-35	22.256421107502554	27.013324887238404	28.864392976553976	21.865861028705062
36-37	22.312131579497045	26.957837587188404	28.776229392024373	21.953801441290175
38-39	22.15102588341168	27.045796410052336	28.727737532330348	22.07544017420563
40-41	22.44388490401702	26.855314439445177	28.743775312694382	21.95702534384342
42-43	22.195414658153297	26.979475090693693	28.491304546467394	22.333805704685613
44-45	22.225617391294147	27.153976413860004	28.28172623536757	22.338679959478277
46-47	22.444572587703153	27.111822033551725	28.00730416574649	22.436301212998632
48-49	22.439634995891367	27.399238501147927	27.705252831427963	22.455873671532746
50-51	22.379491223919675	27.5212761598261	27.454396089459394	22.64483652679483
52-53	22.409505250650128	27.282730479904068	27.41326740784033	22.894496861605482
54-55	22.591725611022152	27.044525083295618	27.744572497666613	22.619176808015613
56-57	22.356040753104704	26.837084714633146	27.856981806853394	22.949892725408752
58-59	22.192273669473337	26.679214365922505	27.91004788931948	23.218464075284682
60-61	22.45256953836631	26.91365995391794	28.09146667763506	22.542303830080687
62-63	22.01469735790685	27.214839236228816	28.044425724269495	22.72603768159484
64-65	21.82651451952335	27.469674042623627	27.72837379047749	22.97543764737554
66-67	21.929249818479697	28.087451918140243	27.255080993319435	22.72821727006063
68-69	22.032924871827785	27.971676491465974	27.133161961624587	22.862236675081654
70-71	22.05843375775675	27.387467092275745	27.108617954083574	23.44548119588393
72-73	22.28639197885462	27.012854080163585	26.988978849153806	23.711775091827985
74-75	22.263229623622472	27.068166652675384	27.310908967898268	23.357694755803873
76-77	22.65749510547894	26.972914181024905	26.668030114281034	23.70156059921512
78-79	22.777298991134703	27.112486719230038	26.16142823499658	23.948786054638678
80-81	22.698119318162714	27.45338311304823	26.300446646527664	23.54805092226139
82-83	22.702752562512526	27.163524480916045	26.465233247781413	23.668489708790013
84-85	22.67140221283682	27.567518562964118	26.39334509171425	23.36773413248481
86-87	23.12377604504089	27.49403867227505	26.504107365726753	22.878077916957306
88-89	22.861946359067186	27.637323722766528	26.772496274219588	22.728233643946698
90-91	22.876580398702913	27.22779675364301	26.674345560201573	23.2212772874525
92-93	22.94864875180871	26.75580810440451	26.901174664122117	23.394368479664656
94-95	22.07032557310289	27.274024975927443	27.55103014309665	23.104619307873016
96-97	22.2165681225102	27.170116036230375	27.69782874447269	22.915487096786737
98-99	23.440982274718998	29.05458902956787	27.942434123821513	19.56199457189162
100	22.50553293429662	30.117588873814206	21.68554180503206	25.691336386857117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4830.0
1	5152.5
2	9365.5
3	13453.0
4	17318.0
5	30915.0
6	42382.5
7	48291.5
8	49787.5
9	43862.0
10	34625.5
11	25327.0
12	25758.5
13	37639.0
14	70033.5
15	117756.0
16	156714.0
17	177276.5
18	178730.5
19	164227.0
20	140382.5
21	115952.0
22	100956.5
23	102808.0
24	120898.0
25	152938.5
26	199154.0
27	250589.0
28	301741.5
29	360089.5
30	413843.0
31	465111.5
32	522565.0
33	577278.0
34	630283.0
35	677781.5
36	733494.0
37	789177.5
38	823183.5
39	850744.0
40	883671.5
41	911609.0
42	930936.5
43	953225.0
44	978660.5
45	1014507.5
46	1040993.5
47	1050249.0
48	1059806.5
49	1059996.5
50	1047553.0
51	1027332.0
52	993197.0
53	938993.5
54	884896.5
55	832003.5
56	775316.5
57	710730.0
58	639593.0
59	563543.0
60	470700.5
61	378766.0
62	299022.0
63	232496.5
64	177754.0
65	133141.5
66	100231.5
67	75197.5
68	55925.0
69	41808.0
70	31086.5
71	22472.5
72	16167.5
73	11510.5
74	7997.5
75	5412.5
76	3661.0
77	2445.5
78	1695.0
79	1223.5
80	877.0
81	591.5
82	437.0
83	298.0
84	193.0
85	141.5
86	108.5
87	84.5
88	63.0
89	49.5
90	36.5
91	23.5
92	17.5
93	10.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.376776099587099E-5
3	0.0
4	0.0
5	0.03130470825840567
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.941367140248944E-6
22-23	2.3107109296355123E-4
24-25	7.575001399918529E-5
26-27	1.4572780575719264E-4
28-29	1.2055070673824009E-4
30-31	5.841816462199845E-6
32-33	1.756996183101492E-5
34-35	2.3501990011419226E-5
36-37	5.369435767298567E-4
38-39	5.5336471438277895E-5
40-41	0.0
42-43	4.007675339503203E-6
44-45	3.0336070267075526E-5
46-47	4.089678302224036E-6
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.1190713656166112E-4
70-71	9.092054770449235E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0012389431321540698
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	882.0
20-21	4295.0
22-23	7059.0
24-25	8533.0
26-27	14163.0
28-29	30040.0
30-31	61607.0
32-33	77135.0
34-35	100089.0
36-37	142214.0
38-39	117357.0
40-41	191002.0
42-43	214970.0
44-45	267518.0
46-47	259523.0
48-49	230358.0
50-51	121718.0
52-53	29579.0
54-55	28153.0
56-57	26897.0
58-59	122423.0
60-61	223833.0
62-63	202998.0
64-65	206765.0
66-67	223626.0
68-69	233397.0
70-71	243388.0
72-73	217667.0
74-75	209604.0
76-77	220459.0
78-79	240160.0
80-81	251475.0
82-83	236116.0
84-85	223987.0
86-87	230783.0
88-89	253102.0
90-91	275652.0
92-93	279626.0
94-95	316895.0
96-97	884913.0
98-99	1675634.0
100-101	1.6850914E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.76995076991919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.37292695164793	64.6870247221027
2	9.076907530420701	13.755136734461704
3	2.6585945729089877	6.04324739719465
4	1.1270942996901272	3.415995184023099
5	0.601279575618826	2.277946192179817
6	0.33459234591096665	1.521122734659943
7	0.21621021401678495	1.1467566090403842
8	0.14024878781349803	0.8501314998535671
9	0.0926077601577582	0.6315196885259241
>10	0.36603736954449334	4.357541658665773
>50	0.009001363243556871	0.4635198246687833
>100	0.0041461838997936444	0.5748427524143453
>500	2.4015195793193812E-4	0.12640931661066818
>1k	1.1289316862305624E-4	0.1488056855987397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.49426236296231E-5	0.0	0.0	0.0
3	3.882513736624789E-6	3.49426236296231E-5	0.0	0.0	0.0
4	3.882513736624789E-6	1.0871038462549409E-4	0.0	0.0	0.0
5	7.765027473249578E-6	1.0871038462549409E-4	0.0	3.882513736624789E-6	0.0
6	7.765027473249578E-6	1.0871038462549409E-4	0.0	3.882513736624789E-6	0.0
7	7.765027473249578E-6	1.1259289836211888E-4	0.0	3.882513736624789E-6	0.0
8	7.765027473249578E-6	1.6306557693824113E-4	0.0	3.882513736624789E-6	0.0
9	7.765027473249578E-6	2.678934478271104E-4	0.0	3.882513736624789E-6	0.0
10-11	1.5530054946499155E-5	2.737172184320476E-4	0.0	1.1647541209874366E-5	0.0
12-13	1.5530054946499155E-5	2.814822459052972E-4	0.0	1.5530054946499155E-5	0.0
14-15	2.5236339288061127E-5	3.688388049793549E-4	0.0	1.9412568683123944E-5	0.0
16-17	3.3001366761310705E-5	3.824276030575417E-4	0.0	2.9118853024685916E-5	0.0
18-19	3.6883880497935494E-5	4.2125274042378955E-4	0.0	3.49426236296231E-5	0.0
20-21	3.882513736624789E-5	4.6784290526328707E-4	0.0	4.270765110287268E-5	0.0
22-23	3.882513736624789E-5	5.396694093908457E-4	0.0	7.959153160080816E-5	0.0
24-25	4.270765110287268E-5	6.037308860451546E-4	0.0	1.1453415523043128E-4	0.0
26-27	4.6590164839497466E-5	6.309084822015282E-4	0.0	1.8830191622630225E-4	0.0
28-29	4.6590164839497466E-5	6.386735096747777E-4	0.0	8.755068476088898E-4	0.0
30-31	4.6590164839497466E-5	7.473838943002718E-4	0.0	0.0034787323080158108	0.0
32-33	5.241393544443465E-5	7.570901786418338E-4	0.0	0.008450291147763852	0.0
34-35	5.629644918105944E-5	8.61918049530703E-4	0.0	0.014629311759602203	0.0
36-37	5.823770604937183E-5	9.667459204195723E-4	0.0	0.028647127605686003	0.0
38-39	5.823770604937183E-5	9.90041002839321E-4	0.0	0.1912856280328984	0.0
40-41	6.212021978599662E-5	0.0010987513874648152	0.0	0.36533289507518274	0.0
42-43	6.600273352262141E-5	0.0011278702404895011	0.0	0.3984216183955675	0.0
44-45	6.600273352262141E-5	0.0012191093133001837	0.0	0.4401411697524692	0.0
46-47	6.794399039093381E-5	0.0012851120468228051	0.0	0.4847143687057901	0.0
48-49	6.98852472592462E-5	0.0012928770742960546	0.0	0.5280218681809712	0.0
50-51	6.98852472592462E-5	0.0014792377336540446	0.0	0.5716516162962924	0.0
52-53	6.98852472592462E-5	0.0014947677886005437	0.0	1.0681746505320266	0.0
54-55	6.98852472592462E-5	0.0015044740729421057	0.0	1.6912191011600215	0.0
56-57	7.182650412755859E-5	0.0015180628710202925	0.0	2.364592577355883	0.0
58-59	7.376776099587099E-5	0.0015432992103083536	0.0	3.078947927298688	0.0
60-61	7.376776099587099E-5	0.0015879481182795386	0.0	3.2736986988415238	0.0
62-63	7.376776099587099E-5	0.0016364795399873484	0.0	3.2816753233134195	0.0
64-65	7.376776099587099E-5	0.0018480765386333995	0.0	3.287654394467822	0.0
66-67	7.376776099587099E-5	0.0018694303641848358	0.0	3.293119032552121	0.0
68-69	7.376776099587099E-5	0.001939315611444082	0.0	3.2983021883905153	0.0
70-71	7.376776099587099E-5	0.001976199491942017	0.0	3.302850553232971	0.0
72-73	7.376776099587099E-5	0.001993670803756829	0.0	3.3064147008431926	0.0
74-75	7.376776099587099E-5	0.0020557910235428256	0.0	3.309332409916266	0.0
76-77	7.376776099587099E-5	0.002170325178773257	0.0	3.312096759696743	0.0
78-79	7.376776099587099E-5	0.002240210426032503	0.0	3.3138011832271212	0.0
80-81	7.376776099587099E-5	0.002259622994715627	0.0	3.314468975589821	0.0
82-83	7.376776099587099E-5	0.002267388022188877	0.0	3.3149465247794256	0.0
84-85	7.376776099587099E-5	0.0022829180771353754	0.0	3.315103766585759	0.0
86-87	7.376776099587099E-5	0.002323684471369936	0.0	3.3151561805212033	0.0
88	7.376776099587099E-5	0.002496456332649739	0.0	3.315158121778072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	39415	0.0	53.300156	1
GTATCAA	76010	0.0	40.012287	1
ATCTCGT	24390	0.0	38.161438	94
TATCTCG	29755	0.0	32.689552	94
ATCAACG	96630	0.0	30.884264	3
TCAACGC	96780	0.0	30.83197	4
TATCAAC	99110	0.0	30.379498	2
CAACGCA	98630	0.0	30.26669	5
AACGCAG	101125	0.0	29.60704	6
TATGCCG	14470	0.0	27.436003	86-87
ACGCAGA	115185	0.0	25.963322	7
CTCGTAT	14935	0.0	25.933308	82-83
TCTCGTA	15105	0.0	25.926744	82-83
ATGCCGT	15885	0.0	25.863117	88-89
CCGTCTT	15855	0.0	25.6874	90-91
CGCAGAG	118085	0.0	25.322071	8
TGCCGTC	16305	0.0	24.85269	88-89
GCCGTCT	16975	0.0	24.599113	90-91
TCGTATG	16590	0.0	24.450916	84-85
GTACATG	110400	0.0	23.791374	1
>>END_MODULE