FastQCFastQC Report
Fri 27 May 2016
SRR2140260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25523718
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA340150.049.7328641
GTATCAA649050.039.2990461
TCAACGC810200.030.7881744
ATCAACG817050.030.5613443
CAACGCA825700.030.1947385
TATCAAC841500.029.9214972
AACGCAG848550.029.503136
ACGCAGA974050.025.6231277
CGCAGAG997650.025.0383438
GTACATG856850.022.2541831
TACATGG860200.021.7514132
GCAGAGT1158500.021.5141359
ACATGGG869200.021.0653693
AGAGTAC1045700.019.54736510-11
CATGGGG630850.019.2640174
GAGTACT656000.018.97107312-13
CAGAGTA1132300.018.1896710-11
AGTACTT717150.017.64153312-13
GTACTTT743250.016.65636814-15
ATGGGGG370700.015.6903825
Produced by FastQC (version 0.11.5)