##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25523718
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.376230061780184	33.0	31.0	34.0	31.0	34.0
2	32.4194808530638	34.0	31.0	34.0	31.0	34.0
3	32.50850726371448	34.0	31.0	34.0	31.0	34.0
4	36.06987551735214	37.0	35.0	37.0	35.0	37.0
5	36.002426331461585	37.0	35.0	37.0	35.0	37.0
6	36.07964666433001	37.0	35.0	37.0	35.0	37.0
7	36.019262044816514	37.0	35.0	37.0	35.0	37.0
8	36.02090114770897	37.0	35.0	37.0	35.0	37.0
9	37.71984489093634	39.0	38.0	39.0	35.0	39.0
10-11	37.65634262218381	39.0	37.0	39.0	35.0	39.0
12-13	37.64545559154038	39.0	37.0	39.0	35.0	39.0
14-15	39.03036775441572	40.0	38.0	41.0	36.0	41.0
16-17	39.026836450708316	40.0	38.0	41.0	35.5	41.0
18-19	39.0595063579687	40.0	38.5	41.0	35.0	41.0
20-21	39.072028208693524	40.0	39.0	41.0	35.0	41.0
22-23	39.03301614088366	40.0	39.0	41.0	35.0	41.0
24-25	38.99734202142645	40.0	38.0	41.0	35.0	41.0
26-27	38.89504667061276	40.0	38.0	41.0	35.0	41.0
28-29	38.8393010243263	40.0	38.0	41.0	35.0	41.0
30-31	38.74030068803752	40.0	38.0	41.0	35.0	41.0
32-33	38.64352444228159	40.0	38.0	41.0	34.0	41.0
34-35	38.59094411994441	40.0	38.0	41.0	34.0	41.0
36-37	38.52481767541123	40.0	38.0	41.0	34.0	41.0
38-39	38.47026125087176	40.0	38.0	41.0	34.0	41.0
40-41	38.421455112925855	40.0	38.0	41.0	34.0	41.0
42-43	38.352417463465756	40.0	38.0	41.0	34.0	41.0
44-45	38.301687419597314	40.0	38.0	41.0	33.0	41.0
46-47	38.225822886308144	40.0	38.0	41.0	33.0	41.0
48-49	38.137520377649956	40.0	37.0	41.0	33.0	41.0
50-51	38.02859606257164	40.0	37.0	41.0	33.0	41.0
52-53	37.92449082431937	40.0	37.0	41.0	33.0	41.0
54-55	37.78038921337568	40.0	36.5	41.0	33.0	41.0
56-57	37.61820509303331	39.5	36.0	41.0	32.5	41.0
58-59	37.43667296793727	39.0	36.0	41.0	32.0	41.0
60-61	37.23404826027881	39.0	35.0	41.0	32.0	41.0
62-63	36.98301529531512	38.5	35.0	40.5	31.5	41.0
64-65	36.72788970837222	38.0	35.0	40.0	31.0	41.0
66-67	36.460448094318906	37.5	35.0	40.0	31.0	41.0
68-69	36.14290631005733	37.0	35.0	40.0	31.0	41.0
70-71	35.823909448678506	36.5	34.0	39.0	31.0	41.0
72-73	35.501199347207205	36.0	34.0	39.0	30.0	40.5
74-75	35.18054173751521	35.5	34.0	38.5	30.0	40.0
76-77	34.85143272738736	35.0	34.0	37.5	30.0	39.5
78-79	34.53186714250874	35.0	34.0	37.0	30.0	39.0
80-81	34.2524483678158	35.0	34.0	36.5	29.5	39.0
82-83	33.96797667942409	35.0	33.5	36.0	29.0	37.5
84-85	33.75234283930529	35.0	33.0	36.0	29.0	37.0
86-87	33.550714544419165	35.0	33.0	35.0	29.0	37.0
88-89	33.419044175716834	35.0	33.0	35.0	29.0	36.0
90-91	33.29197185266614	35.0	33.0	35.0	29.0	36.0
92-93	33.172285223088615	35.0	33.0	35.0	29.0	36.0
94-95	33.40365893380306	35.0	33.0	35.0	29.5	36.0
96-97	33.46241588075838	35.0	33.0	35.0	30.0	35.0
98-99	33.79420478460465	35.0	34.0	35.0	31.0	35.0
100	33.60393135824947	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	24.0
20	115.0
21	704.0
22	3398.0
23	11600.0
24	30667.0
25	65733.0
26	117921.0
27	186906.0
28	267309.0
29	354978.0
30	452466.0
31	565059.0
32	708985.0
33	911337.0
34	1243455.0
35	1756817.0
36	2825956.0
37	5273496.0
38	6884932.0
39	3691541.0
40	170315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60190533369786	20.197566827842245	13.782067330472778	24.41846050798712
2	14.610081849450452	21.63698117317676	40.87073616706675	22.882200810306035
3	17.444613672663206	27.471377798485314	30.778517455803268	24.305491073048213
4	12.303509230120783	18.603480887854975	39.2128803491717	29.88012953285254
5	12.719509122897833	38.47517043951911	35.75342897395794	13.05189146362512
6	28.93161176596607	38.874579322651975	19.15065430514473	13.043154606237225
7	25.55668809693008	33.08044306084247	24.099753021875575	17.263115820351878
8	24.536711305147627	36.49981558329394	21.390719800304954	17.57275331125348
9	26.03329969403361	16.097819291060965	20.96455931694591	36.90432169795952
10-11	24.084710150770356	26.516736315610444	29.376335375590656	20.022218158028544
12-13	25.299725141924856	24.29078906137421	29.24252454129136	21.16696125540958
14-15	22.431890604652505	25.215826706751738	26.8575781161663	25.494704572429455
16-17	21.848509688126157	28.283060093361005	27.358288866849257	22.510141351663577
18-19	21.956924535837608	27.492221156807954	29.105130765039792	21.445723542314642
20-21	22.92393880599626	26.58977193057348	28.852023433005293	21.63426583042497
22-23	22.62880656823459	26.5696513074745	28.70465478558202	22.096887338708886
24-25	22.31674905099731	26.793170696923944	28.721858395907475	22.16822185617127
26-27	22.288986322746048	26.855871581608078	28.681790395883194	22.173351699762684
28-29	22.310413469517854	26.82422333748343	28.67561101493163	22.18975217806708
30-31	22.569400379300316	26.733532184735914	28.556887512441964	22.140179923521806
32-33	22.389310336284417	26.942813148465504	28.522192170322857	22.145684344927215
34-35	22.488304145659967	26.919114196674123	28.51006482824097	22.082516829424943
36-37	22.5304036436388	26.883215586588964	28.40781351929586	22.178567250476373
38-39	22.45008837861534	26.916576572671495	28.441913453896824	22.191421594816344
40-41	22.641761915754024	26.81482350476051	28.29611686765367	22.247297711831795
42-43	22.47008045847366	26.955599273613256	28.103158190843896	22.471162077069188
44-45	22.531278889293933	27.06588540112965	27.9278647546016	22.47497095497482
46-47	22.73295297123704	27.07984203309789	27.526540335796355	22.660664659868715
48-49	22.696317124854595	27.220428402068038	27.39028915168433	22.69296532139304
50-51	22.706539194534813	27.35097216287562	27.246064031415397	22.69642461117417
52-53	22.815021358908965	27.26614836722495	27.151592342265367	22.76723793160071
54-55	22.766300887159648	27.140485451018076	27.298070698130072	22.795142963692197
56-57	22.744006483398586	27.207686936953774	27.288652315499956	22.759654264147688
58-59	22.742061422547852	27.264016369433385	27.25075769482896	22.7431645131898
60-61	22.83754781766355	27.28723111385033	27.24518638223568	22.630034686250443
62-63	22.797375639393287	27.445486163739968	27.12262410414567	22.634514092721076
64-65	22.75097792374757	27.342460879895736	27.098776004023588	22.807785192333103
66-67	22.804415940651655	27.394809163086677	26.991634849649298	22.80914004661237
68-69	22.74067609588779	27.29510926889052	27.089314975627087	22.874899659594604
70-71	22.796795139819757	27.071149434966234	27.132191552161412	22.9998638730526
72-73	22.852042689133558	27.138357243669418	26.99042407943604	23.01917598776099
74-75	22.692859305642045	27.303465947266336	27.06826126733101	22.935413479760605
76-77	22.94305915426009	27.193012646634724	26.978652912217306	22.885275286887882
78-79	22.770916221658688	27.240952574172532	26.911251200258114	23.076880003910667
80-81	22.87140785330433	27.263143633144676	26.914865196836917	22.95058331671408
82-83	22.852924246229808	27.231669728282444	27.05791663540352	22.857489390084226
84-85	22.923252114781917	27.302868718475082	26.91497685414851	22.8589023125945
86-87	22.772194474337514	27.304700710679363	27.058325555493308	22.86477925948982
88-89	22.79846938629296	27.251670814635165	27.00592514358693	22.94393465548495
90-91	22.89867306229606	27.271372608554195	26.999178447893396	22.830775881256343
92-93	23.000857576896898	27.224687089750887	26.97223769900677	22.80221763434545
94-95	22.75396076017044	27.301306806214864	27.137115175866374	22.807617257748326
96-97	22.813828436056145	27.387326694430698	27.154187054100227	22.644657815412934
98-99	23.890738392076972	29.328301603963858	27.29500825804167	19.4859517459175
100	22.93250744265665	30.228205498381012	21.106976168630194	25.73231089033215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5421.0
1	5618.0
2	9037.5
3	12366.0
4	15964.5
5	28356.5
6	38674.5
7	43752.0
8	44480.0
9	38930.0
10	30935.5
11	22825.0
12	23062.5
13	33460.5
14	62315.0
15	104809.0
16	139647.5
17	158545.0
18	160452.5
19	148171.5
20	128077.0
21	107786.0
22	95677.0
23	98870.5
24	116665.0
25	147852.5
26	189593.5
27	235911.5
28	287601.5
29	345803.5
30	401553.5
31	454090.0
32	509400.5
33	562216.0
34	611682.0
35	658132.0
36	709569.5
37	763882.5
38	807875.0
39	838940.0
40	872659.5
41	908494.5
42	935467.0
43	960736.5
44	988768.5
45	1025701.0
46	1051155.5
47	1060528.5
48	1070537.0
49	1071313.5
50	1060869.0
51	1046338.5
52	1017126.5
53	967181.0
54	914830.0
55	856973.5
56	797369.5
57	735296.5
58	662228.5
59	580577.0
60	485784.0
61	394052.0
62	314872.5
63	243171.5
64	180406.0
65	133785.5
66	100585.5
67	76014.5
68	57549.5
69	43321.5
70	32579.5
71	24324.0
72	17898.5
73	12998.0
74	9134.5
75	6172.0
76	4201.5
77	2910.5
78	2020.0
79	1394.5
80	973.0
81	676.0
82	501.5
83	358.5
84	244.5
85	175.0
86	128.0
87	89.5
88	63.5
89	42.5
90	28.5
91	20.5
92	15.0
93	11.5
94	7.5
95	4.0
96	3.0
97	2.5
98	2.0
99	2.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.0970188590862821E-4
3	0.0
4	0.0
5	0.031037797863148305
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.918156374208619E-6
22-23	2.1554422663809205E-4
24-25	8.820129684130771E-5
26-27	1.4705775375634033E-4
28-29	1.432307536912135E-4
30-31	1.964818278280042E-6
32-33	1.5755285750663543E-5
34-35	1.975133542729091E-5
36-37	5.829470846241281E-4
38-39	4.984634763648359E-5
40-41	0.0
42-43	8.08686734996248E-6
44-45	3.8741638152651064E-5
46-47	2.0597894252553087E-6
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	9.913769804923823E-5
70-71	1.38788772634458E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	7.47598675673802E-6
90-91	0.0
92-93	0.0
94-95	0.001195002163964873
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	861.0
20-21	4360.0
22-23	6960.0
24-25	8505.0
26-27	13952.0
28-29	27920.0
30-31	56535.0
32-33	69279.0
34-35	89927.0
36-37	129481.0
38-39	165427.0
40-41	173222.0
42-43	197099.0
44-45	244490.0
46-47	235387.0
48-49	210320.0
50-51	179713.0
52-53	155871.0
54-55	164002.0
56-57	181644.0
58-59	191255.0
60-61	199538.0
62-63	184473.0
64-65	187887.0
66-67	202521.0
68-69	210362.0
70-71	219628.0
72-73	198612.0
74-75	194766.0
76-77	202528.0
78-79	219993.0
80-81	230126.0
82-83	219019.0
84-85	209414.0
86-87	216651.0
88-89	236918.0
90-91	258982.0
92-93	266006.0
94-95	308019.0
96-97	832169.0
98-99	1540368.0
100-101	1.6679528E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.3427923352651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.09776832652888	66.59041816208487
2	8.76002594987028	13.55049735784701
3	2.5016889986332744	5.804628381261319
4	1.0422385004719255	3.224385436232729
5	0.5366843774212607	2.0754334176236804
6	0.31707178598339464	1.4713930379211189
7	0.2025453238796247	1.0965794646310578
8	0.13423746101770556	0.8305840056884516
9	0.08544267811848083	0.5947537779257905
>10	0.3104516222520566	3.7607899410060113
>50	0.008074551402214691	0.4315631760178439
>100	0.0036761789980340005	0.5062884201517759
>500	6.734729865259279E-5	0.035858981726540444
>1k	2.6898124212668233E-5	0.026826439881839574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1753773490210164E-5
2	0.0	1.5671697986946886E-5	0.0	3.9179244967367214E-6	1.1753773490210164E-5
3	7.835848993473443E-6	1.5671697986946886E-5	0.0	3.9179244967367214E-6	1.1753773490210164E-5
4	7.835848993473443E-6	5.093301845757738E-5	0.0	1.1753773490210164E-5	1.1753773490210164E-5
5	7.835848993473443E-6	5.876886745105082E-5	0.0	1.1753773490210164E-5	1.1753773490210164E-5
6	7.44405654379977E-5	5.876886745105082E-5	0.0	1.1753773490210164E-5	4.3097169464103934E-5
7	7.44405654379977E-5	5.876886745105082E-5	0.0	1.5671697986946886E-5	4.3097169464103934E-5
8	8.619433892820787E-5	7.44405654379977E-5	0.0	3.5261320470630495E-5	4.701509396084066E-5
9	8.619433892820787E-5	1.3320943288904852E-4	0.0	4.3097169464103934E-5	6.660471644452426E-5
10-11	1.1361981040536492E-4	1.3712735738578526E-4	0.0	9.403018792168131E-5	8.031745218310278E-5
12-13	1.1753773490210164E-4	1.3712735738578526E-4	0.0	1.1361981040536492E-4	9.598915017004967E-5
14-15	1.3320943288904852E-4	1.841424513466259E-4	0.0	1.351683951374169E-4	1.1753773490210164E-4
16-17	1.8218348909825754E-4	1.9197830034009935E-4	0.0	1.4104528188252198E-4	1.2145565939883837E-4
18-19	1.8610141359499428E-4	2.0373207383030952E-4	0.0	1.449632063792587E-4	1.2145565939883837E-4
20-21	1.9393726258846772E-4	2.1548584732051967E-4	0.0	1.6259386661457393E-4	1.2145565939883837E-4
22-23	2.1156792282378295E-4	2.6641886577809704E-4	0.0	2.1352688507215133E-4	1.292915083923118E-4
24-25	2.1156792282378295E-4	2.997212240003592E-4	0.0	2.546650922878869E-4	1.3908631963415363E-4
26-27	2.1156792282378295E-4	3.09516035242201E-4	0.0	3.565311292030417E-4	1.449632063792587E-4
28-29	2.1156792282378295E-4	3.1735188423567443E-4	0.0	7.992565973342912E-4	1.449632063792587E-4
30-31	2.1352688507215133E-4	3.604490536997784E-4	0.0	0.002730793374225495	1.4692216862762707E-4
32-33	2.1548584732051967E-4	3.604490536997784E-4	0.0	0.006580154192269324	1.7630660235315248E-4
34-35	2.1940377181725641E-4	4.152999966540925E-4	0.0	0.011242484343386022	1.9589622483683606E-4
36-37	2.1940377181725641E-4	4.7210990185677493E-4	0.0	0.019685611633853656	2.0373207383030952E-4
38-39	2.1940377181725641E-4	5.034532978306686E-4	0.0	0.04634904679639541	2.252806585623615E-4
40-41	2.1940377181725641E-4	5.543863162882461E-4	0.0	0.07795886163606729	2.3115754530746656E-4
42-43	2.1940377181725641E-4	5.91606599007245E-4	0.0	0.0951761808369768	2.350754698042033E-4
44-45	2.3115754530746656E-4	6.660471644452427E-4	0.0	0.114481753794647	2.566240545362553E-4
46-47	2.4291131879767674E-4	7.130622584060833E-4	0.0	0.13497641683707678	2.703367902748338E-4
48-49	2.4487028104604506E-4	7.1698018290282E-4	0.0	0.15438385583166214	2.958032995036225E-4
50-51	2.5858301678462366E-4	8.090514085761331E-4	0.0	0.17369922360057416	3.0168018624872757E-4
52-53	2.6250094128136035E-4	8.188462198179748E-4	0.0	0.22610342270667622	3.3694150671935804E-4
54-55	2.6250094128136035E-4	8.247231065630798E-4	0.0	0.28316995196389494	3.7807971393509363E-4
56-57	2.6250094128136035E-4	8.345179178049217E-4	0.0	0.3334271284457852	3.898334874253038E-4
58-59	2.6250094128136035E-4	8.521485780402369E-4	0.0	0.35820800088764493	3.976693364187772E-4
60-61	2.683778280264654E-4	8.658613137788154E-4	0.0	0.376661425267275	4.1138207215735573E-4
62-63	3.0168018624872757E-4	8.776150872690255E-4	0.0	0.39083255817197166	4.192179211508292E-4
64-65	3.0168018624872757E-4	9.559735772037601E-4	0.0	0.39721093925265905	4.192179211508292E-4
66-67	3.0559811074546426E-4	9.71645275190707E-4	0.0	0.40317206137444395	4.231358456475659E-4
68-69	3.0559811074546426E-4	0.0010167014069031792	0.0	0.40914885519421584	4.2705377014430265E-4
70-71	3.153929219873061E-4	0.0010304141426417577	0.0	0.41401491741916285	4.3097169464103934E-4
72-73	3.6632594044488345E-4	0.0010441268783803362	0.0	0.41758610559793835	4.623150906149331E-4
74-75	3.682849026932518E-4	0.0010852650855960719	0.0	0.42038742161310516	4.642740528633015E-4
76-77	3.7220282718998857E-4	0.0011479518775438595	0.0	0.4230320206484024	4.7994575085024836E-4
78-79	3.7612075168672526E-4	0.001167541500027543	0.0	0.4247323998799861	4.936584865888269E-4
80-81	3.7612075168672526E-4	0.0011714594245242798	0.0	0.425418036666915	4.99535373333932E-4
82-83	3.7612075168672526E-4	0.0011851721602628583	0.0	0.42592148996474577	5.21083958065984E-4
84-85	3.7612075168672526E-4	0.001226310367478594	0.0	0.42609975552934726	5.328377315561941E-4
86-87	3.8003867618346195E-4	0.001271366499191066	0.0	0.42617811401928196	5.328377315561941E-4
88	3.839566006801987E-4	0.0013947811208382729	0.0	0.4261839909060271	5.406735805496676E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	34015	0.0	49.732864	1
GTATCAA	64905	0.0	39.299046	1
TCAACGC	81020	0.0	30.788174	4
ATCAACG	81705	0.0	30.561344	3
CAACGCA	82570	0.0	30.194738	5
TATCAAC	84150	0.0	29.921497	2
AACGCAG	84855	0.0	29.50313	6
ACGCAGA	97405	0.0	25.623127	7
CGCAGAG	99765	0.0	25.038343	8
GTACATG	85685	0.0	22.254183	1
TACATGG	86020	0.0	21.751413	2
GCAGAGT	115850	0.0	21.514135	9
ACATGGG	86920	0.0	21.065369	3
AGAGTAC	104570	0.0	19.547365	10-11
CATGGGG	63085	0.0	19.264017	4
GAGTACT	65600	0.0	18.971073	12-13
CAGAGTA	113230	0.0	18.18967	10-11
AGTACTT	71715	0.0	17.641533	12-13
GTACTTT	74325	0.0	16.656368	14-15
ATGGGGG	37070	0.0	15.690382	5
>>END_MODULE